Resveratrol Alters microRNA Expression Profiles in A549 Human Non-Small Cell Lung Cancer Cells

배승희,이은미,차화준,김가람,윤영민,이현진,김종란,김유정,이홍기,정회경,민유홍,안성관 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.3

Resveratrol is a plant phenolic phytoalexin that has been reported to have antitumor properties in several types of cancers. In particular, several studies have suggested that resveratrol exerts antiproliferative effects against A549 human non-small cell lung cancer cells; however, its me-chanism of action remains incompletely understood. Deregulation of microRNAs (miRNAs), a class of small, non-coding, regulatory RNA molecules involved in gene expression, is strongly correlated with lung cancer. In this study, we demonstrated that resveratrol treatment altered miRNA expression in A549 cells. Using microarray analysis, we identified 71 miRNAs exhibiting greater than 2-fold expression changes in resveratrol-treated cells relative to their expression levels in untreated cells. Furthermore, we identified target genes related to apoptosis, cell cycle regulation, cell proliferation, and differentiation using a miRNA target-prediction program. In conclusion, our data demonstrate that resveratrol induces considerable changes in the miRNA expression profiles of A549 cells, suggesting a novel approach for studying the anticancer mechanisms of resveratrol.

A Case of Sebaceous Carcinoma of the Submandibular Gland

배승희,김민재,이성민,박혜상 대한이비인후과학회 2018 대한이비인후과학회지 두경부외과학 Vol.61 No.5

Sebaceous carcinoma of the salivary gland is an extremely unordinary malignancy occurringmainly in the parotid gland. Sebaceous carcinoma arising in the submandibular gland is exceptionaland only three cases have been reported in the literature. We herein present a uniquecase of sebaceous carcinoma of the submandibular gland, the first to be reported in literaturein South Korea. An 85-year-old man visited our hospital complaining of a progressively enlargingsubmandibular mass with tenderness and ipsilateral facial palsy. We performed a wideexcision of the mass with superficial parotidectomy and modified neck dissection. Microscopicand immunohistochemical examinations demonstrated sebaceous carcinoma of low gradedifferentiation. Further adjuvant radiotherapy was rejected and the patient was followed up forfive months. The optimal treatment of sebaceous carcinoma of the salivary gland is still notestablished due to the rarity of the disease. When more cases are reported, the clinicopathologiccharacteristics will be better understood.

현장Essay-조화

배승희,Bae, Seung-Hui 한국단미사료협회 2006 피드저널 Vol.4 No.4

구강내 장치를 사용한 폐쇄성 수면무호흡증 환자에서 장기간 사용 시 나타나는 합병증 분석

배승희,김민재,이성민,이호준,박혜상,이준호,박찬흠,김동규 대한이비인후과학회 2017 대한이비인후과학회지 두경부외과학 Vol.60 No.9

Background and Objectives Recently, mandibular advancement device (MAD) has been used widely used for the treatment of obstructive sleep apnea (OSA) because it can effectively reduce the collapsibility of upper airway during sleep. Although MAD is widely prescribed by otorhinolaryngologists, several studies described its complications arising from the anterior placement of the mandible with long-term use. However, there is still a lack of studies on long-term complications in Korean patients. Subjects and Method Retrospectively, we included a total of 57 OSA patients in the study. In this study, all enrolled OSA patients had used MAD over two years with more than 4 hours/day. Dental consulting and cephalometric analysis were conducted to identify the change of dental and skeletal findings at two different times (baseline and after 2 year). Results The dental findings showed that the overbite and overjet were significantly decreased. Additionally, the proclination of the lower incisors were increased significantly, whereas there was no significant change on the retroclination of the upper incisors. Three patients showed a change of malocclusion type. On the analysis of skeletal findings, we found a significant increase in the sella turcica central point-nasion-supramental point angle and a decrease in the subspinal point-nasion-supramental point angle. Moreover, the anterior lower facial height and anterior facial height were significant increased. Conclusion Consistent with Western studies, our findings suggest that the long-term use of MAD could induce changes in dental and skeletal morphologies in Korean OSA patients. Therefore, clinicians should thoroughly evaluate potential changes in dental and skeletal morphologies when they prescribe MAD to patients with OSA. Korean J Otorhinolaryngol-Head Neck Surg 2017;60(9):449-53

Notch Signaling in Hair Follicle Development

배승희,이기헌,이인호,김미경,이재호 한국피부과학연구원 2017 아시안뷰티화장품학술지 Vol.15 No.3

Many studies have represented that Notch activity is critical to determine cell fate decision during hair follicle development. Also, growing results have revealed the pivotal role of Notch signaling pathway in controlling of hair follicle stem cell fates, regulation of hair cycles and resuscitation of injured hair organs. Furthermore, in hair bulb, Notch signaling pathway regulates hair follicle stem cell differentiation. The purpose of this review is to summarize considerable recent evidences in our understanding of Notch signaling and their regulating factors in hair follicle development to maintain their hair follicle homeostasis.

보이스피싱 대화의 대화 원형 연구: 수사기관 사칭형을 중심으로

배승희,최민경,이혜용 한국사회언어학회 2023 사회언어학 Vol.31 No.4

This study examined the dialog pattern in the structure and progress of conversations by analyzing actual voice phishing conversations. The study aimed to reconstruct the Dialog Pattern by synthesizing the structure of the conversation and the pattern of the development process found in several actual conversations. The dialog pattern of voice phishing is divided into six functional phases: approaching the victim, threatening criminal involvement, social isolation, vertical relationship enhancement, induction of withdrawal, and check withdrawal. The process of the voice phishing criminal exercising power over the victim and attempting financial fraud by unilaterally leading the conversation was demonstrated in each derived phase. This study provides basic data for the study of voice phishing conversations and can be used in education related to the prevention of voice phishing damage in the future.

초등학교 영어수업에서 문자지도의 병행이 학습효과에 미치는 영향

배승희 한국영어언어과학학회 조기영어교육연구소 1999 English Linguistic Science Vol.- No.2

H2AX의 BRCA1 NLS domain과 BARD1 BRCT domain 각각과의 in vitro 상호 결합

배승희(Seunghee Bae),이선미(Sun-Mi Lee),김수미(Sumi Kim),최태부(Tae-Boo Choe),김차순(Cha Soon Kim),성기문(Ki-Moon Seong),진영우(Young-Woo Jin),안성관(Sungkwan An) 한국생물공학회 2009 KSBB Journal Vol.24 No.4

본 연구에서는 H2AX의 생리학적인 기능 및 분자세포 생물학적 기전 해석에 대한 보다 명확한 정보를 제시하고자, H2AX 관련 단백질들을 literature review 및 생물정보학적인 기술을 이용하여 최적의 결합 단백질체를 40개를 예측하고, 이들 가운데 상호작용 가능성이 높은 BRCA1 와 BARD1 단백질을 선별하여 in vitro 결합실험을 통해 이를 증명하였다. 이들 두 가지의 유전자를 발굴하여, 클로닝하였다. 클로닝된 유전자를 이용하여 두 가지 단백질을 발현 및 정제하였으며, 단백질들의 자체적인 구조에 의한 결합능력을 판단하기 위해 in vitro binding assay법을 실시하였다. 단백질의 구조적 안정과 비특이적 결합을 억제하는 detergent만이 포함된 상태에서, 구조학적 및 물리학적 상호 결합의 유무를 판정할 수 있게 하였으며, BRCA1과 BARD1은 모두 H2AX에 결합함을 확인하였다. 이런 실험 결과를 바탕으로 각각의 단백질에 대해 H2AX와의 최적 결합 부위를 알아내기 위해 각 유전자의 domain을 생물정 보학적으로 분석하였다. 이에 RING domain, NES, NLS 및 BRCT domain에 해당하는 유전자 부분을 새로 클로닝하여, 다시 in vitro 결합실험 및 실험결과에 대한 literature review를 통한 분석을 실시한 결과, H2AX는 BRCA1의 NLS, BARD1의 BRCT domain 부분과 결합하는 것을 확인하였다. H2AX에 대한 BRCA1과 BARD1과의 결합은 DNA repair에 있어 BRCA1의 NLS와 BARD1의 BRCT domain을 통해 H2AX foci의 관련 세포 신호전달 기전에 중요한 역할을 하여 전체적으로 genomic stability에 영향을 미칠 가능성이 농후할 것으로 사료된다. H2AX, a crucial component of chromatin, is implicated in DNA repair, cell cycle check point and tumor suppression. The aim of this study was to identify direct binding partners of H2AX to regulate cellular responses to above mechanisms. Literature reviews and bioinformatical tools were attempted intensively to find binding partners of H2AX, which resulted in identifying two potential proteins, breast cancer-1 (BRCA1) and BRCA1-associated RING domain 1 (BARD1). Although it has been reported in vivo that BRCA1 co-localizes with H2AX at the site of DNA damage, their biochemical mechanism for H2AX were however only known that the complex monoubiquitinates histone monomers, including unphosphorylated H2AX in vitro. Therefore, it is important to know whether the complex directly interacts with H2AX, and also which regions of these are specifically mediated for the interaction. Using in vitro GST pull-down assay, we present here that BRCA1 and BARD1 directly bind to H2AX. Moreover, through combinational approaches of domain analysis, fragment clonings and in vitro binding assay, we revealed molecular details of the BRCA1-H2AX and BARD1-H2AX complex. These data provide the potential evidence that each of the BRCA1 nuclear localization signal (NLS) and BARD1 BRCA1 C-terminal (BRCT) repeat domain is the novel mediator of H2AX recognition.

Evaluation of Citrus junos Essential Oil as a Promising Antiaging Ingredient: In Vitro and Clinical Studies on Skin Health improvement

김별,배승희 한국피부과학연구원 2023 아시안뷰티화장품학술지 Vol.21 No.3

Purpose: This study assessed Citrus junos essential oil (CJEO) as an anti-aging agent for improving skin health. Methods: CJEO was extracted from authenticated C. junos fruit peels, and its chemical composition was analyzed using a gas chromatographymass spectrometry (GC-MS). In vitro experiments were conducted on human epidermal keratinocytes (HEKs) and human dermal fibroblasts (HDFs) to evaluate CJEO’s impact on cell viability, anti-inflammatory activity, collagen synthesis, and collagen degradation. The expression of TNF-α , IL-1β , IL-6 , NF-κB , MMP-1 , MMP- 3 , COL1A1 , and COL3A1 was quantified using RT-qPCR. A dermal patch test was performed on human participants to assess potential skin irritation, and clinical evaluations were conducted on 20 female participants to analyze CJEO’s effects on skin elasticity. Results: CJEO effectively inhibited nitric oxide production and significantly suppressed the expression of inflammatory markers, including TNF-α , IL-1β , IL-6 , and NF-κB , as well as collagen degradation markers MMP-1 and MMP- 3 . Furthermore, CJEO treatment upregulated the expression of genes associated with collagen synthesis, specifically COL1A1 and COL3A1 . Notably, significant improvements in skin elasticity were observed in the 20 female participants. The dermal patch test indicated excellent tolerance of the CJEO emulsion without causing irritation. Conclusions: CJEO exhibits promising potential as a bioactive ingredient in anti-aging cosmeceuticals. Its anti-inflammatory properties, ability to stimulate collagen synthesis, suggest efficacy in reducing skin aging and promoting overall skin health. Additionally, the dermal patch test confirms CJEO’s safety, supporting its suitability for use in cosmetic formulations. These findings endorse CJEO as an effective and safe component in anti-aging skincare products.

빅데이터를 활용한 색조화장품의 구매 요인에 관한 연구: 토픽모델링과 Concor 분석을 중심으로

이은희,배승희 한국응용과학기술학회 2023 한국응용과학기술학회지 Vol.40 No.4

본 연구에서는 코로나 이후 색조화장품 시장의 소비자들의 온라인 관심 정보에 대한 자료 수집을 통하여 색조화장품 정보 검색의 특성과 텍스트 마이닝 분석 결과에 나타난 코로나 이후 색조화장품 시장의 주요 관심정보들을 분석하고자 하였다. 실증분석에서는 “색조화장품” 이라는 단어를 포함하는 뉴스, 블로그, 카페, 웹페이지 등의 모든 문서들을 분석 대상으로 텍스트 마이닝을 수행하였다. 분석 결과 코로나이후 색조화장품에 대한 온라인 정보 검색은 주로 구매 정보와 피부와 마스크 관련 화장법 등에 관한 정보와 관심 브랜드와 행사 정보 등의 주요 토픽이 주를 이루고 있었다. 결과적으로 코로나 이후 색조화장품구매자들은 적극적인 온라인 정보 검색을 통하여 제품 가치와 안전성, 가격 혜택, 매장 정보 등의 구매 정보에 더욱 민감하게 될 것이므로 이에 대한 대응전략이 요구된다.