http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Microarray와 Network 분석을 통한 병원균 및 스트레스 저항성 관련 주요 유전자의 대량 발굴
김형민,문수윤,이진수,배원실,원경호,김윤경,강권규,류호진,Kim, Hyeongmin,Moon, Suyun,Lee, Jinsu,Bae, Wonsil,Won, Kyungho,Kim, Yoon-Kyeong,Kang, Kwon Kyoo,Ryu, Hojin 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.3
브라시노스테로이드는 식물의 생장과 발육 과정에 있어서 중요한 역할을 담당 할 뿐 아니라 생물학적/ 비 생물학적 스트레스에 대한 복합 저항성을 보인다고 알려져 있다. 따라서 본 연구에서는 브라시노스테로이드와 광범위스트레스 내성을 연결하는 중요한 생물학적 네트워크를 이해하기 위해, Agilent Arabidopsis $4{\times}44K$ oligo chip을 이용하여 브라시노스테로이드 신호가 강화된 bes1-D 계통의 전 전사체 비교분석을 수행하였다. 그 결과 bes1-D 계통에서 DEGs (Differentially Expressed Genes)를 1,091 (562 up-regulated, 529 down-regulated) 개 선발하였다. 또한 선발된 유전자들의 GO 와 단백질 상호작용 네트워크 분석을 통해 대사, 발달, 스트레스, 면역, 방어 반응에 관련된 주요 브라시노스테로이드 신호전달과 연결된 스트레스 관련 유전자군을 분리하였다. 선발된 유전자중 NB-ARC와 FLS2는 bes1-D 계통이 야생형 En-2 계통에 비해 약 6배 정도의 발현량이 증가되었으며, TIR1, TSA1, OCP3 유전자등은 bes1-D 계통이 야생형 En-2 계통에 비해 발현이 감소되었다. 또한 브라시노스테로이드 활성형 계통이 야생형 식물체 계통에 비해 가뭄 스트레스 및 병원균에 대해 저항력이 향상되었다. 따라서 microarray 분석을 통한 유전자 간 발현 네트워크와 유전체 정보를 결합하여 대단위 주요 기능 유전자들을 동정할 수 있는 방법을 고안하여 실험에 사용하였다. 이를 통해 기능 획득 돌연변이 bes1-D가 식물들이 다양한 스트레스 환경에 적응할 수 있는 반응을 조절한다는 사실을 보여주고 있다. Brassinosteroid (BR), a plant steroid hormone, plays key roles in numerous growth and developmental processes as well as tolerance to both abiotic and biotic stress. To understand the biological networks involved in BR-mediated signaling pathways and stress tolerance, we performed comparative genome-wide transcriptome analysis of a constitutively activated BR bes1-D mutant with an Agilent Arabidopsis $4{\times}44K$ oligo chip. As a result, we newly identified 1,091 (562 up-regulated and 529 down-regulated) significant differentially expressed genes (DEGs). The combination of GO enrichment and protein network analysis revealed that stress-related processes, such as metabolism, development, abiotic/biotic stress, immunity, and defense, were critically linked to BR signaling pathways. Among the identified gene sets, we confirmed more than a 6-fold up-regulation of NB-ARC and FLS2 in bes1-D plants. However, some genes, including TIR1, TSA1 and OCP3, were down-regulated. Consistently, BR-activated plants showed higher tolerance to drought stress and pathogen infection compared to wild-type controls. In this study, we newly developed a useful, comprehensive method for large-scale identification of critical network and gene sets with global transcriptome analysis using a microarray. This study also showed that gain of function in the bes1-D gene can regulate the adaptive response of plants to various stressful conditions.
tun-우언적 구문(1) - 현대 (표준)독일어의 tun-우언적 구문
김형민 ( Kim Hyeongmin ) 한국독일언어문학회 2023 독일언어문학 Vol.- No.100
Der vorliegende Aufsatz beschäftigt sich mit der tun-Periphrase in der modernen deutschen (Standard)sprache, in der das Verb tun als Hilfsverb/Auxiliar funktioniert. Die periphrastische tun-Konstruktion ist die sprachliche Ausdrucksweise, die in den regionalen Varietäten der modernen deutschen Sprache und kommunikativen Situationen mit starker Mündlichkeit oft auftritt. In den Verbzweitsätzen der modernen deutschen (Standard)sprache lässt sich die tun-Periphrase in drei Typen gliedern: 1. [V<sub>INF</sub>]<sub>VF</sub> + [TUN<sub>Ind. FIN</sub>]<sub>LSK</sub> + [...], 2. [[TUN<sub>Ind. FIN</sub>]<sub>LSK</sub> + [...] + [V<sub>INF</sub>]<sub>RSK</sub>]<sub>SK</sub>, 3. [[TUN<sub>Konj.Ⅱ FIN</sub>]<sub>LSK</sub> + (...) + [V<sub>INF</sub>]<sub>RSK</sub>]<sub>SK</sub>. Der erste Typ der tun-Periphrase, nämlich [V<sub>INF</sub>]<sub>VF</sub> + [TUN<sub>Ind. FIN</sub>]<sub>LSK</sub> + [...], wird als Konstruktion mit Verbtopikalisierung bezeichnet, die für standardsprachliche Ausdrucksweise gilt. Den zweiten Typ der tun-Periphrase, d.h. [[TUN<sub>Ind. FIN</sub>]<sub>LSK</sub> + [...] + [V<sub>INF</sub>]<sub>RSK</sub>]<sub>SK</sub>, bezeichnet man als Konstruktion mit Verbrhematisierung. Diese tun-Periphrase mit nachgestelltem Verb wird als ‘substandardlich’ oder ‘nicht korrekt’ bewertet. Dabei wird das Verb in normativen Grammatiken als ‘überflüssiges Hilfsverb’ bezeichnet. Der dritte Typ der tun-Periphrase, genau gesagt, [[TUN<sub>Konj.Ⅱ FIN</sub>]<sub>LSK</sub> + (...) + [V<sub>INF</sub>]<sub>RSK</sub>]<sub>SK</sub> oder periphrastisches ‘täte + [...] + V<sub>INF</sub>’, kann als alternative Konstruktion von den konjunktivischen Präteritalformen des Vollverbs und dem analytischen ‘würde + [...] + V<sub>INF</sub>’ verstanden werden. Da diese periphratische tun-Konstruktion häufig in den regional begrenzten Varietäten des Deutschen vorkommt, wird sie als ‘landschaftlich’ eingeordnet.
학문 목적 한국어 발표 교육을 위한 세종구어말뭉치 발표 담화 분석
김형민(Kim Hyeongmin) 학습자중심교과교육학회 2019 학습자중심교과교육연구 Vol.19 No.9
본 연구의 목적은 세종구어말뭉치의 발표 담화를 분석하여 한국인 대학생의 발표 에서 많이 쓰이는 어휘와 표현을 추출하고 이를 교육에 활용하는 방안을 모색하는 것이다. 이를 위해서 세종구어말뭉치의 발표 담화 10개를 연구 대상으로 정하고 말뭉치 분석 프로그램 Antconc를 이용하여 단어 및 표현의 빈도, 빈도가 높은 표현인 ‘-(으)ㄹ 수 있-’과 결합하는 동사 및 어미의 형태, 발표 담화와 일상 대화의 차이 등을 분석하였다. 분석 결과, 한국인의 발표에서 지시사와 간투사, 담화표지의 빈도가 높게 나타났고, 가장 많이 나타난 표현은 ‘-(으)ㄹ 수 있-’이었다. 또한, 발표 담화와 일상 대화의 차이를 분석한 결과, 발표 담화에서는 격식체를 사용하고 축약과 조사 생략이 일상 대화보다 많이 나타나지 않으며 발표의 응집성을 위한 담화표지와 주제를 나타내는 표현을 많이 사용한다는 것을 알 수 있었다. The purpose of this study is to analyze the presentation discourse of the Sejong Spoken Corpus, to extract vocabulary and expressions commonly used in the presentation of Korean college students and to find ways to utilize them in Korean education. For this purpose, ten speeches of Sejong Spoken Corpus were selected as research subjects and the corpus analysis program Antconc was used to analyze the frequency of words and expressions, the forms of verbs and suffixes combined with ‘-(으)ㄹ 수 있-’ the most frequent expression, and the differences between academic oral presentation and daily conversation. As a result of the analysis, the characteristics of the Korean presentation were seen. The analysis showed a high frequency of indicators, interjections, Discourse markers, and the most frequent expression is ‘-(으)ㄹ 수 있-’. In addition, the analysis of the differences between oral presentation and daily conversation indicated that formal speech was used, abbreviations and omissions were not shown more than daily conversation. Lastly, it was found that discourse markers are used for the coherence of the presentation and the expressions to express the topic are used a lot.
김형민 ( Hyeongmin Kim ),이선영 ( Sunyoung Lee ),박영지 ( Youngji Park ) 이중언어학회 2020 이중언어학 Vol.80 No.-
The purpose of this study is to examine the trends of web-based research in the field of Korean language education in order to suggest the direction of future research. This study analyzed 282 web-based Korean education studies based on three criteria: research general, research subject, and research topic. First of all, in ‘research general’, web-based Korean education research has grown quantitatively and qualitatively since the late 90s. In particular, as of 2010, when the use of smart devices became common, web-based Korean education research showed marked increase. Next, in ‘research subjects’, there were an overwhelmingly large number of studies for general purpose learners and all levels. Lastly, in ‘research topics’, the ‘teaching-learning method’ of research using the web was most frequently conducted. Also, in the teaching-learning tool, studies regarding MALL were rapidly increasing. Through this analysis, this study suggested the direction of future research as follows. First, future research should be able to verify their educational effectiveness. Second, in order to verify them, commercialization is needed through collaboration with educational engineering, computer engineering, and AI-related businesses. Third, research is required based on various learning purposes, needs, and variables of Korean learners. Fourth, it is necessary to study ways to increase the learning motivation of self-learning users. Fifth, there is a need for research on ways to activate the interaction between teachers and learners on the web. Sixth, it is necessary to study application development that fully reflects the specificity and advantages of MALL. (Korea University, The Cyber University of Korea, Korea University)
한국어 읽기 텍스트 분류를 위한 오픈소스 인공지능 기술 성능 평가
김형민 ( Kim Hyeongmin ),고현준 ( Ko Hyunjun ) 국제한국어교육학회 2022 한국어 교육 Vol.33 No.2
The purpose of this study is to evaluate the level of technical completeness of classifying text by subject through analysis of Korean natural language processing(NLP) open-source software related to the currently developed text classification. To this end, after searching for the released NLP software, the accuracy was evaluated using Naver news articles. As a result, it was analyzed that Simple RNN had an accuracy of 78% and LSTM of 80%. In addition, the model was evaluated using < Cyber Korean Intermediate 1&2 >, and it was difficult to determine the accuracy because the training data was different from the evaluation data. However, if text classification technology is applied to Korean language education based on this study, the significance of this study can be found in that it is possible to efficiently manage vast amounts of Korean reading materials and provide texts tailored to learners’ interests. (Korea University·Igalia)
실시간 화상 한국어 수업에서의 상호작용 활성화를 위한 온라인 도구 활용 방안
김형민 ( Kim Hyeongmin ),이선영 ( Lee Sunyoung ) 한국화법학회 2021 화법연구 Vol.- No.52
This study aims to examine the diverse types of available online tools in synchronous Korean language classes and propose practical application methods. Research on synchronous Korean classes in Korean language education began at the start of the COVID-19 pandemic, and studies have demonstrated that active interactions are necessary for successful online classes. Applications such as Zoom, Google Meet, and Webex are used by default in online classes, but Google Docs, Google Forms, Padlet and Mentimeter can also be used to promote interaction. In this paper, these tools were utilized for small group conversations, simple response displays, data retrieval and sharing, memo boards, questionnaires/ statistics, mind maps, and quizzes. To demonstrate each tool’s application in the Korean classes, a section of “Sejong Korean Conversation” was used as an example. Before the class, Zoom’s annotation function or Padlet’s memo function was used to enhance familiarity and bring attention to the teacher’s presence. During the introduction phase, Mentimeter’s survey, wordcloud, and mind-mapping function were used to generate interest in the topic and activate background knowledge. At the activity/task level, Google Docs was used for engaged discussion. In the final phase, Padlet and Google Survey were used to receive feedback about the lesson or perform a self-assessment.