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김형국(Hyung Kuk Kim),이동규(Dong Gyu Lee),백종현(Jong Hyeon Peck),강채동(Chaedong Kang) 대한설비공학회 2013 설비공학 논문집 Vol.25 No.9
A numerical analysis of solid-liquid phase change was performed on a heat transfer module which consisted of circulating water path (BRINE), heat transfer plate (HTP) and phase change material (PCM) layers, such as high temperature PCM (HPCM, 78~79℃) and low temperature PCM (LPCM, 28~29℃). There were five arrangements, consisting of BRINE, HTP, LPCM and HPCM layers in the heat transfer module. The time and heat transfer rate for melting/solidification was compared to their arrangements, against each other. As results, the numerical time without convection was longer than the experimental one for melting/solidification. Moreover, the melting/solidification with the BRINEⅠ-LPCM-BRINEⅡ- HPCM arrangement was faster(10 hours) than the others; HPCM-BRINE-LPCM, BRINEⅠ-HPCM-LPCM-BRINEⅡ one.
생쥐 1-세포기배의 초급속 동결에 있어서 평형 온도와 노출시간의 영향
정덕수,김형국,박인국,Chung, Duk-Soo,Kim, Hyung-Kuk,Park, In-Kook 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.3
The present study was to assess the effect of ultrarapid freezing on the development of 1-cell mouse zygote using cryoprotectants, DMSO (dimethyl sulfoxide) or PROH (1,2-propanediol). We investigated the effect of the type and concentration of cryoprotectant, and of the temperature and time of prefreezing equilibration on their capacity to develop to the blastocyst stage in vitro. The concenration, the equilibration temperature, and the exposure time seemed to serve as an important factor in ultrarapid freezing of 1-cell mouse zygotes. In addition to the exposure time and the concentration of cryoprotectant appeared to playa key role in the development of the embryo. In general, the development of the embryo was more effective at $3^{\circ}C$ than $23^{\circ}C$ and 4.5 M than 3 M for 3 to 5 minutes. At $23^{\circ}C$ the development of the embryo was stimulated by DMSO while at $3^{\circ}C$ it was stimulated by PROH. Thus it has been suggested that there exists a correlation between the concentration of cryoprotectants and exposure time in the development of the embryo. In conclusion, we found that for ultrarapid freezing of mouse 1-cell embryos in DMSO, or PROH-based solution, viability shown optimum depending on the cryoprotectant, the concentration of the cryoprotectant and on the temperature and the duration of equilibration.