독소루비신을 함유하고 단백질로 수식된 양이온성 리포솜의 제조 및 혈장 단백흡착 특성

김성규,정순화,정석현,성하수,지상철,조선행,신병철,Kim, Sung-Kyu,Jung, Soon-Hwa,Jung, Suk-Hyun,Seong, Ha-Soo,Chi, Sang-Cheol,Cho, Sun-Hang,Shin, Byung-Cheol 대한화학회 2008 대한화학회지 Vol.52 No.1

나노 또는 마이크로 크기를 가지는 구형의 약물 전달체이다. 그러나 일반적인 리포솜은 혈류 순환시 혈장 단백질과의 흡착이 일어나 안정성이 떨어지고, 세망내피계의 대식세포에 의해 옵소닌작용이 일어나 혈중에서 쉽게 소실되는 단점이 있다. 이에 본 연구에서는 모델단백질로 소혈청 알부민(BSA)을 사용하였고, BSA의 등전점보다 높은 pH를 나타내는 수용액에 용해하여 BSA가 음이온성을 갖도록 제조하였으며 이를 양이온성 리포솜 표면에 정전기적 인력에 의해 결합시켰다. 그리고 리포솜 표면에 코팅된 알부민을 60oC 이상의 온도에서 변성시켜 알부민이 코팅된 리포솜을 제조하였다. 대조 리포솜과 양이온성 리포솜의 입자크기는 104±1nm를 나타내었고, 변성된 알부민이 결합된 리포솜은 109±1nm의 입자크기를 나타내었다. 모델약물로서는 독소루비신(doxorubicin, DOX)을 사용하였고, 이온구배에 의한 리모트 로딩 방법을 사용하여 리포솜 내부에 DOX를 봉입시켰다. 혈장 내에서 리포솜의 안정성을 평가한 결과, 알부민이 결합된 리포솜은 입자크기의 변화가 관찰되지 않았고, 대조 리포솜과 양이온 리포솜에 비해 단백질 흡착이 억제되어 변성된 알부민으로 코팅된 리포솜은 혈류 내에서 장기 순환이 가능한 약물전달체로서 유용할 것이라 사료된다. are nanometer or micrometer scale vesicles that can be used as drug delivery carriers. However, plain liposomes are plagued by rapid opsonization, making their circulation time in bloodstream be shortened. In this study, model protein, bovine serum albumin (BSA)-coated liposomes were prepared by coating cationic liposomes with BSA molecules at higher pH than isoelectric point of BSA. The BSA molecules coated on the liposomal surface were denatured by thermal treatment at above 60oC. While both plain and cationic liposomes had about mean particle diameter of 1041 nm, BSA-coated cationic liposomes (BCL) had mean particle diameter of 1091 nm. Encapsulation of model drug, doxorubicin (DOX), in liposomes were carried out by using remote loading method and the loading efficiency of DOX to liposomes was about 90%. The mean particle diameter of BCL did not increase in blood plasma and adsorption of plasma protein was much less than plain or cationic liposomes. These results suggest that BCL can be used as a long-circulating liposomes in bloodstream.

미국의 해기사 교육제도와 취업현황에 관한 연구

김성규,Kim, Seong-Kyu 한국항해항만학회 2001 한국항해학회지 Vol.25 No.2

This paper presents the educational methodology shipboard training methods of Maritime Colleges in U.S.A. and the fields of engagement in business of the graduates from them. There are two Maritime Academies, i.e. U.S. Merchant Marine Academy (USMMA) and U.S. Coast Guard Academy, which are supported by federal government and six Maritime Colleges operated by each State. Maritime Administration (MARAD) in the Department of Transportation (DOT) supports the training of merchant marine officers and crew members with a focus on safety in U.S. waterborne commerce. The shipboard training methods are not unique, but have various process. One is continuous sea term training onboard during sophomore (100 days) and junior year (200 days) in case of USMMA, the other is summer sea term training onboard every three months during freshman, sophomore and junior years in case of SUNY Maritime College. They offer also one month ship simulator training ashore. The employment status for the 1999 year graduates from USMMA shows 43 percent in the field of maritime afloat, 34 percent of maritime ashore, 22 percent of active duty military and 1 percent of others.

해운물류정보(海運物流情報)시스템의 활성화요인(活性化要因)이 물류성과(物流成果)에 미치는 영향(影響)에 관한 연구(硏究)

김성규,조성무,이용호,안기명,Kim, Seong-Kyu,Cho, Sung-Mou,Lee, Yong-Ho,An, Ki-Myung 한국항해항만학회 2001 한국항해학회지 Vol.25 No.2

The information technology(IT) development of telecommunication, along with the information analyzation over the world, enables the firms to process their inner and outer operations through Electronic Data Interchange (EDI) system. And the concept of logistics has become more important to the enterprises in strategic sense because it had aggrandized itself to more comprehensive and integrative concept. Also the world shipping market is more competitive than any other market. In these environment, to maintain competitive power, shipping firms process operations by Logistics Information system(LIS), And then, reduce the working processes, working time and its costs. So the LIS has become more important in developing our national logistics system and industry. Therefore, this study purposes on empirically analyzing the activation factors of shipping LIS and the relationship these, factors with logistic performances in the shipping industry. Considering environments of Shipping logistics information system, this study sets up the research model and hypothesis to examine the activation factors of shipping LIS and the relationship these factors with logistic performances. We used the field data on the 163 numbers of firms(29 overseas shipping companies and 133 forwarding Agency) to get the empirical evidence. According to the study results, The shipping LIS activation factors which the sample firms recognized as important: were top managers support, well structured communication channel, strategic plans connection and inner information analyzation level. And IS organization & human resources, IS discipline & training, software development, IS organizational alignment, database management system, information standardization, competitive advantage, financial support and government support represent partially significant. Also, the current shipping LISs main problems are expensive system cost, standardization of working processs, institutions imperfection and systems interaction defect. So these problems must be improved to activate shipping LIS.

Propofol 마취유도 시 기관내 삽관에 따른 심혈관계 변동을 최소화하기 위한 Remifentanil의 효과처 농도는?

김성규 ( Seong Kyu Kim ),김덕규 ( Deok Kyu Kim ),손지선 ( Ji Seon Son ),고성훈 ( Seong Hoon Ko ),이준례 ( Jun Rae Lee ) 대한마취과학회 2008 Korean Journal of Anesthesiology Vol.54 No.1

중세국어의 형태 분석과 성조

김성규(Seong Kyu Kim) 한국어학회 2007 한국어학 Vol.37 No.-

향가의 구성 형식에 대한 새로운 해석

김성규(Kim, Seong-kyu) 국어국문학회 2016 국어국문학 Vol.- No.176

향가는 형식면에서 4구체, 8구체, 10구체로 분류되어 왔다. 그러나 이러한 분류는 土田杏村(1929), 小倉進平(1929) 등이 편의상 붙인 명칭을 답습한 것이다. 향가 자체는 기원적으로는 그렇게 구분되어 있지는 않았을 수도 있다. 여기서는 10구체 향가의 제9구 앞에 기록되어 있는 “後句, 後言, 隔句, 落句”라는 시 형식에 대한 표현을 문제 삼아, ‘隔句’ 등으로 표현된 부분이 後斂이었을 가능성을 제시하였다. 그리고 ‘後句, 隔句, 落句’에 보이는 ‘句’의 길이를 시 형식 설정에 적극적으로 이용하여, 두 개의 句로 나뉘었던 단위를 하나의 句로 이해하였다. 이러한 재해석을 통해 기존에 ‘4+4+2(後句)’로 이해되던 10구체 향가가 원래는 ‘3(2+후렴)+3(2+후렴)’의 구성으로 이루어져 있었을 가능성을 타진하였다. Hyangga (鄕歌) has been classified as four, eight and ten line-forms. This classification is the scholarly interpretation of the later researchers, however, it would not have been so from the outset. Considering the expressions hwukwu (後句, literally post-phrase), hwuen (後言, literally post-word), kyekkwu (隔句, literally isolated phrase), and nakkwu (落句, literally fallen phrase), which are believed to indicate the form of the poem, written in front of the beginning of the line nine, I present the possibility that they, in fact, may have represented the chorus. In addition, actively taking advantage of the length of each kwu (句) in setting up the form of poems, I interpret the two consecutive lines as one. Through the reconstruction, I argue that ten-line Hyangga form which consists of two four-line stanzas and one concluding two-line hwukwu ‘4+4+2’ could be rearranged into two three-line stanzas where each stanza comprises a two-line verse and a single-line chorus ‘3(2+chorus)+3(2+chorus)’.

황지우 시의 서술과 해체, 그리고 현실의 전복

김성규(Kim, Seong-kyu) 충남대학교 충청문화연구소 2013 충청문화연구 Vol.11 No.-

The focus of this paper is to examine his attitude of composing poems and sense of direction shown in the first collection of poems of Hwang, Ji-woo, Birds Also Leave The World. ‘Anatomy of Criticism’, written by Northrop Frye, and his work of criticism is the material of intertextuality chosen to study and advance this paper. The combination of Frye and Hwang seems rather strange, unfamiliar and disparate like Hwang’s poems. However, I confirmed a possibility of the combination of both parties as follows: First, the gloomy reality in their literary works is described as the same image of winter. Second, although his poetry shows twisted language structure, the narrative method of Hwang can be explained by Frye’s view of irony and satire. Third, both authors pursue a liberal, classless and civilized society through either literature or criticism. Hwang, Ji-woo’s early poems reveal negative reality frankly through the narration depart from the standard pattern. It is composed in the way ingeniously twisted and disintegrated by irony and satire. This can be a series of process to move towards the utopia of spring, free from the painful awareness of reality, that is to say, Frye’s image of winter. Hwang’s poetry has great significance that he added his flexible intelligence to the rigidity of Korean popular literature in the 1980’s, studded with the poetry of work or job site, furthermore, his poetry was the indelible imprint of Korean literature that tried to straighten the distorted and heart-rending Korean history committed by a certain institution.

음성 언어 층위와 문자 언어 층위의 위상에 대한 연구

김성규(Seong Kyu Kim) 사단법인 한국언어학회 2001 언어학 Vol.0 No.30

15세기 국어와 표준어의 음운론적 유사도 측정 시론

김성규 ( Kim Seong-kyu ) 국어학회 2015 국어학 Vol.75 No.-

“15세기에 살던 사람과 현대인이 만난다면 어느 정도 의사소통을 할 수 있을까?” 이 질문에 대해 우리가 줄 수 있는 답변은 “중세국어와 현대국어를 비교해 보니 ‘음운, 어휘, 형태, 문법, 의미’에서 이러이러한 점이 차이가 난다.” 정도일 것이다. 그러나 이러한 진술은 복잡하기도 하거니와, 일반인이 듣고 싶어 하는 속 시원한 답변이 아니다. 이 상황에서 제시되어야 할 답변은 좀 더 간단하고 명료한 진술이다. 명료한 진술은 차이를 수치화하는 것이므로, 이 논문에서는 ‘방언 측정법(Dialectometry)’을 원용하여 중세국어와 현대국어의 음운론적인 유사도를 수치화해 보고자 하였다. 서로 다른 언어 체계 사이의 유사도를 재는 방법은 다양하게 고안될 수 있겠지만, 여기서는 두 시기의 단어들이 보이는 형태 유사도를 기반으로 중세국어와 현대국어의 음운론적인 유사도를 제시하였다. “To how much degree can 15th century Korean and present day Korean communicate with each other?” The answer we can give would be that “the results of the comparison show that there are such and such differences in phonology, vocabulary, morphology, grammar and semantics.” This sort of statement is, however, not only a complicated answer, but also a dubious one that can not answer laymen’s inquiry; a simpler and clearer statement is necessary. Since the clearer statement is to present the specific figures, I use the dialectometrical method to measure the phonological similarity between Middle Korean and Present Day Korean. Although the linguistic distance between different language systems can be measured in a number of ways, this study presents the phonological distance between Middle Korean and Present Day Korean based on the morphological similarity of words between the two periods.

표준문법 정립을 위한 음운 분야의 논점 탐색

김성규 ( Seong Kyu Kim ),김소영 ( So Young Kim ) 한국문법교육학회 2013 문법 교육 Vol.19 No.-

This paper aims to analyze phonological issues of school grammar and grammar for establishing standard grammar. This work is needed because both of them has become developed differently, and differences of them are very huge. In this paper I analyze two kinds of school grammar textbooks: <munbeop> and <dokseowa munbeop>, argue that phonological knowledge and pronunciation education should be regarded as distinguishable things in education fields. Furthermore contents of school grammar should be comprised vocal language data with synchronic perspectives. Moreover I present main phonological arguments which are found among 16 korean phonology textbooks, that are composed of position of diphthong / / in phonological system and the number of monophthongs and so on.