Serratia liquefaciens AL-11이 생산하는 Alkaline Lipase의 특성 및 작용양상

최청,김태완,안봉전,김영활,손준호,김성,최희준 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

본 효소의 최적 반응온도는 약 45℃이고, 최적 pH는 10.0 정도였고, pH 7.0~10.0 범위와 30~50℃의 범위에서 안정하였다. 금속이온중 Mn^2+, Ca^2+ 등에 의하여 활성이 증대되었으나 Fe^2+, Pb^2+와 Zn^2+ 등에 의해서는 효소 활성이 저해되었고, 효소활성 저해제 중 ethylenediaminetetraacetic acid(EDTA)에 의해 강한 저해작용을 나타내어 본 효소는 효소분자 중 금속이온이 관여하는 것으로 추정되었다. 효소반응 처리한 olive oil 가수분해물을 박충크로마토그래피 분석한 결과 Serratia liquefaciens AL-11이 생산하는 지방분해효소는 기질특이성이 비특이적이었으며, sodium cholate, sodium edoxychol-ate, sodium taurocholate 등의 담즙산염에 의해 효소활성이 증대되었다. The optimum temperature and pH for the enzyme activity were 45℃ and 10.0, respectively. The enzyme was stable in a pH range of 5 to 10, and 62% of its activity was lost on heat treatment at 60℃ for 20 min. The activity of the purified enzyme was inhibited by Fe^2+, Zm^2+ and Pb^2+, and slightly activated by Mn^2+ and Ca^2+. γ-Chloromercuribenzoic acid, 2,4-dinitrophenol and H_2O_2 did not show inhibitory effect on the lipolytic activity of the alkaline lipase but ethylenediaminetetraacetic acid inhibited the enzyme activity. This suggested that the enzyme have metal group in its active site. Sodium salts of bile acids stimulated the enzyme activity. Analysis of hydrolyzates of olive oil after the lipase reaction revealed that Serratia liquefaciens AL-11 produced non-specific lipolytic enzyme.

21C 신지식인 양성을 위한 EQM 시스템 구축

최병태,이형민,전상봉,서승교,이우언,백종현 대구산업정보대학 2001 논문집 Vol.15 No.-

The aim of this paper is to form and to operate EQM, to say education quality management. In order to achieve the above stated purpose, the paper hypothesizes that there are similarities between enterprises and colleges in quality management. The basic method of the paper is systematic approach, modifies TQM, which is total quality management. The result is that EQM program must form and operate the feedback system for teaching and learning effect, the PSMT(project, self-study, media, team unit style teaching and learning) as a new teaching and learning method, and the digital system as an advanced mechanism, in education quality management.

지역사회 엘리트의 행태에 관한 연구 : 대구지역 엘리트의 정책참여태도를 중심으로

조용상,최봉기,김옥준 계명대학교 사회과학연구소 2000 한국사회과학연구 Vol.19 No.1

熱處理 硬化層을 고려한 有限要素解析의 試圖

최윤용,조상봉,김태규 慶南大學校 附設 工業技術硏究所 2003 硏究論文集 Vol.21 No.1

표면을 열처리한강은 어떤 깊이에 대하여 일반적으로 경도의 변화층을 가지고 있다고 잘 알려져 있다. 이것은 기계적 특성이 다른 두 재료를 붙여 놓은 것과 같은 이종재료간 접합구조물로 생각할 수 있을 것이다. 열처리 후에는 재료의 성질이 변화하는 것으로 알려져 있고, 경도 및 인장강도 등이 변화하는 물성치의 예이다. 그런데 재료의 탄성계수와 푸아송의 비가 열처리 후에 달라지는지에 대한 연구 결과는 찾기가 어렵다. 본 연구에서는 SM45C강 환봉의 열처리한 후에 탄성계수와 푸아송 비의 변화에 대하여 실험적으로 규명하고 열처리 경화층을 이종재료의 층으로 고려하여 탄성 응력해석해야 할지를 한 가지의 해석모델을 설정하여 검토하였다. In general, it is well known that the surface heat-treated steel has a certain depth of hardened layer with different hardness. The surface heat-treated steel can be thought as a dissimilar structure bonded by two materials which have different mechanical properties. Material properties are changed by heat treatment and hardness, tensile strength etc. are examples of changing material properties. To author's knowledge, it is difficult to find out the results of study for elastic modulus and Poisson's ratio of heat-treated materials. In this paper. Elastic modulus and Poisson's ratio for the heat-treated SM45C steel rod were investigated experimentally. Elastic finite element analysis was also performed to investigate that the surface heat-treated hardened layer must be considered as a dissimilar structure.

地域社會開發에서의 成人敎育의 位置와 展開問題에 관한 硏究

崔成鳳 건국대학교 행정대학원 1976 建國大學校 行政大學院 硏究論叢 Vol.4 No.-

매실박, 당근박, 감귤박 급여가 산란계 난황의 Vitamin E, K 함량에 미치는 영향

최승현,나재천,권오상,박상미,이봉덕,안길환 충남대학교 농업과학연구소 2008 농업과학연구 Vol.35 No.2

This experiment is a quantitative experiment where insoluble Vitamins E and K are extracted from the yolk of the spawning which was fed by by-products of tangerine, carrot, or Japanese apricot. Finally the purpose is to raise contents of the insoluble Vitamins E and K inside the yolk of the spawning. The saponification method through α, α - dipyridyl was used to determine the content of Vitamin E. Diethyl dithiocarbamate assay was used to examine the content of Vitamin K. The contents of Vitamin E in egg yolks of chicken fed by by-products of tangerine, carrot, or Japanese apricot increased 2 - 3 times compared to control egg, while the contents of Vitamin K almost no changed. 본 실험에서는 감귤, 매실, 당근의 껍데기로 감귤박, 매실박, 당근박을 먹여 산란계의 난황에 들어있는 Vitamin E와 K 함량을 높이고자 했다. Vitamin E 추출방법에는 검화방법인 α, α -dipyridyl에 의한 비색정량법을 사용하였으며 전처리 과정 중 석유 ether를 가하여 층을 분리하는 과정과 증류수를 가하여 층을 분리하는 과정에 있어서 신중을 가하였고 신속한 시료 전처리과정이 요구되었다. Vitamin K 추출에는 Diethyl dithiocarbamate에 의한 방법이 사용되었다. 실험결과 일반난황에서의 Vitamin E 함량보다 실험난황에서의 Vitamin E 함량이 2배 이상 높아졌음을 알 수 있었던 반면 Vitamin K의 함량은소량 증가하여 큰 변화가 없었다. 위의 결과를 통해서 난황에 이전된 Vitamin E의 종류가 무엇이며 어떠한 형태의 Vitamin E가 이전이 많이 되었는지를 확인하는 실험이 필요할 것이다. 이러한 추가적인 실험이 이루어졌을 때 상업적으로 이용한 가치가 있는 가를 고려하여야 할 것이다.

태권도 우수선수들의 정신력 특성

최상진,조옥성,백우석,이갑철,고봉수 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

The purpose of this study was to investigate the mental toughness of Taekwondo players and to suggest way of performance enhancement using Loehr's Psychological Performance Inventory. Subjects were 188 Taekwondo elite players. Each factor's mean and standard deviation of Mental Toughness were calculated, and ANOVA were used for statistical analyses of the difference of mean. The results are as follow;: 1. On the level of self-confidence, it shows that the man players is higher than the woman players (school career F=5.846, P<.05: sex distinction F=8.876, P<.01). 2. On the level of attention control, it shows that the woman players is higher than the man players (school career F=4.483, P<.05: sex distinction F=9.814, P<.01). 3. On the level of visual and imagery control, it shows that the man players is higher than the woman players (school career F=7.790, P<.01: sex distinction F=5.000. P<.05). 4. On the level of arousal control, motivation, positive energy and attitude control, it was not significant difference according to the sexual and school career.

허혈성 심질환에 있어서 아데노신 급속 정맥투여후 관동맥압의 변화에 관한 연구

봉종대,오종용,배성한,신원용,김철현,이광희,최태명,현민수,김성구,권영주 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

Background: Adenosine interacts with A1 receptors present on the extracellular surface of cardiac cells, activating K+channels in a fashion. It produces coronary vasodilatation and AV nodal block. The half-time is one to six second and has ultra-short action. In ischemic heart disease, fractional flow reserve(FFR) can be achieved safely with intravenous adenosine infusions at a rate of 150㎍/kg/min which cause maximal coronary hyperemia. Purpose: We investigated the patients who have had ischemic heart disease(n=8). After adenosine intravenous administration(150㎍/kg/min), the effect cause maximal hyperemia of coronary artery. During peak maximal hyperemia, we reported clinical findings, coronary hemodynamics and electrocardiogram findings. Methods: After diagnostic coronary angiography and left ventriculography, catheter was advanced into the ostium of coronary artery. We investigated coronary hemodynamics such as systolic coronary artery pressure, diastolic coronary artery pressure, mean coronary artery pressure and heart rate. To assess the use of adenosine for determination of fractional flow reserve, hemodynamics and electrocardiogram were measured at basal state and during peak maximal hyperemia. Results: Symptoms developed 1.5-2 minute after adenosine intravenous administration and each symptom disappeared 2-2.5 minute after adenosine administration. Two patients represented asymptomatic appearance and six patients complained of flushing, chest pain, palpitation and headache. In eight patients with ischemic heart disease, 1.5 minute after venous administration of 150㎍/kg/min of adenosine, coronary artery pressure decreased and heart rate increased significantly. Conduction abnormalities after administration of 150㎍/kg/min of adenosine were transient 2nd degree AV block 2 cases(25%). Conclusions: Adenosine is a potent ultra short-acting vasodilator. Although coronary artery pressure decreased and heart rate increased and mild cardiovascular symptoms occurred after continuous infusion of 150㎍/kg/min of adenosine, it is useful as an agent for determination of fractional flow reserve.

메타인지 전략을 활용한 수업이 실과의 사고 기능에 미치는 영향

최지연,정성봉 韓國實科敎育硏究學會 2002 實科敎育硏究 Vol.8 No.1

The purpose of this study was to apply three-staged metacognition strategy(plan-monitor-control) based teaching on chair making task with corrugated cardboard in an elementary practical course to verify the effect of metacognition strategy-adopted teaching on thinking skill. 80 6th grade students from B elementary school in A city, Kyonggi province were enrolled to verity the hypothesis. A post inspection control group design was used among the designs which use the existing class structure. The metacognition strategy-adopted teaching (self-plan, self-monitoring, and self-controlling) was used on the experimental group and classic teaching method was applied on the control group. The effect was verified by post inspection method. To verify the effect of post evaluation results, t-score method was used and significance level was set as p<0.5 For statistical analysis SPSS/WIN 10.0 was used for statistical treatment. The result showed that the group which used the metacognition strategy-applied teaching came up with better performance on thinking skill in a practical arts than the control group. The conclusion derived from the above study is that metacognition strategy-adopted teaching was more effective in thinking skill in a practical arts than the class method. The study suggests that a better learning efficiency can be achieved by applying metacognition strategy in the thinking skill of a practical arts.

中等農業敎師에 要求되는 敎師像

崔成鳳 건국대학교 농과대학 농업교육학과 1975 農業敎育硏究 Vol.2 No.-