골개조와 LED(Light Emitting Diode) 광 조사의 효과

김옥준 대한치과의사협회 2006 대한치과의사협회지 Vol.44 No.10

한중관계에 있어서의 북한의 변수

김옥준 啓明大學校 社會科學硏究所 1999 한국사회과학연구 Vol.18 No.2

Analysis of Inflammatory Cytokines by Exposure of Hyperbaric Oxygen on P.gingivalis LPS Treated Human Gingival Fibroblast

김옥준,임원봉,김인애,김지선,고영종,권혁일,김상우,차현록,김서연,최홍란 대한구강악안면병리학회 2010 대한구강악안면병리학회지 Vol.34 No.4

Human gingival fibroblasts (hGFs) were reported to play an important role in inflammatory reactions to lipopolysaccharide (LPS) from P.gingivalis in the periodontal connective tissue. Although the biostimulatory effects of hyperbaric oxygen therapy, such as anti-inflammatory activity, have been reported, the pathological mechanism is not completely understood. This study examined the changes in the inflammatory cytokine profiles, which are produced after exposure to hyperbaric oxygen in P.gingivalis LPS-treated human gingival fibroblasts, and subsequently to examine the mitogen activated protein kinase (MAPK) pathway involved in cytokine production. Gingival fibroblasts with or without P.gingivalis LPS were exposed to hyperbaric oxygen, and the cytokine profiles in the supernatant were observed using a human inflammation antibody array. The expression of cyclooxyginase-2 (COX-2) protein, phosphorylation of extracellular signal-regulated kinase (ERK1/2), p38, and c-Jun-N-terminal kinase (JNK) MAPK by western blot analysis, and the amount of prostaglandin E2 (PGE2) in the supernatant by an enzyme-linked immunoassay were determined. COX-2 protein expression and PGE2productionwereincreasedsignificantlyintheP. gingivalis LPS-treated group, and were decreased by treating P. gingivalis LPS with hyperbaric oxygen. Treatment of P. gingivalis LPS in the gingival fibroblasts led an increase in the amount of pro-inflammatory-related cytokines interleukin-6 (IL-6) and IL-8 released, whereas hyperbaric oxygen inhibits the irrelease. Ananalysis of the MAPK signal transduction showed that hyperbaric oxygen induced a significant decrease in the level of P38 phosphorylation regardless of the presence or absence of LPS. In addition, hyperbaric oxygen promoted JNK phosphorylation, significantly in the presence of LPS. Hyperbaric oxygen can inhibit pro-inflammatory cytokines and mediate the MAPK signal pathway, and appears to be useful as an anti-inflammatory tool.

경주(慶州)망간광산(鑛山) 조사보고(調査報告)

김옥준,Kim, Ok Joon 대한자원환경지질학회 1970 자원환경지질 Vol.3 No.2

위에서 상논(詳論)한것을 요약(要約)하면, 1) 본(本) 지성(地城)을 구성(構成)하는 지질(地質)은 신나통(新羅統)의 퇴적암류(堆積岩類), 이를 관입(貫入)하는 불국사순(佛國寺純)의 화강록암(花崗綠岩) 및 암맥(岩脈)으로 되어 있다. 2) 광구일대(鑛區一帶)의 퇴적암류(堆積岩類)는 원내(原來)의 퇴적상(堆積相)과 화강섬록암(花崗閃綠岩)의 관입등(貫入等)으로 규화(珪化)되 어 규암(珪岩) 또는 규화(珪化)된 혼펠스로 변(變)하여 있다. 3) 지질구조(地質構造)는 단순(單純)한 배사(背斜)를 나타내 며 퇴적암(堆積岩) 및 화강섬록암중(花崗閃綠岩中)에 수(數) sets의 절이(節理)가 발달(發達)하여 이들에 따라 광화작용(鑛化作用)이 이루어졌다. 4) 광상(鑛床)은 퇴적암(堆積岩) 및 화강섬록암중(花崗閃綠岩中)의 열하에 연(沿)하여 배태(胚胎)하며 연(軟)망간광(鑛) 광맥(鑛脈)과 금은광맥(金銀鑛脈)의 2종(種)이 발달(發達)된다. 5) 금은광맥(金銀鑛脈)은 맥폭(脈幅)이 좁고 주향방향(走向方向)의 연장(延長)이 단속(斷續)되 며 분석결과(分析結果) 금(金)은 없고 은(銀)은 23.3g/t 내지(乃至) 913.3g/t 이여서 가행가치(稼行價直)가 없다. 6) 망강광맥(鑛脈)은 수조(數條)있으나 주목(注目)만한 것은 제(第)4갱맥(坑脈)과 제(第)5갱맥(坑脈)으로서, 전자(前者)는 연장(延長) 120m, 평균맥폭(平均脈幅) 0.5m 추정(推定)되며, 평균품위(平均品位)는 Mn 약(約) 17.5%이다. 후자(後者)도 연장(延長) 120m, 평균맥폭(平均脈幅) 0.6m 추정(推定)되며, 평균품위(平均品位)는 15.7%이다. 7) 광맥(鑛脈)은 후화강섬록암시기(後花崗閃綠岩時期)의 열하의 방위(方位)에 지배(支配)받으며, 주향(走向) 및 경사방향(傾斜方向)으로 전변(轉變)한다. 그러나 규암질모암(珪岩質母岩)의 성질(性質)로 보아 광상(鑛床)이 팽대(膨大)하여질 가능성(可能性)은 없다. 8) 망간 및 금은광맥(金銀鑛脈)은 열수광상(熱水鑛床)에 속(屬)한다.

임플란트와 연조직의 종양

김옥준 대한치과의사협회 2008 대한치과의사협회지 Vol.46 No.2

뇌졸중후 우울증에서 호모시스테인의 역할

김옥준 대한뇌졸중학회 2003 Journal of stroke Vol.5 No.1

Department of Neurology, College of Medicine, Pochon CHA University Institute for Clinical Research, College of Medicine, Pochon CHA University* Department of Preventive Medicine, College of Medicine, Pochon CHA University**Background : The risk of depression after stroke is influenced by various factors such as age, severity of disability, the location of the brain lesion, etc. We examined whether plasma homocysteine and MTHFR genotypes are associated with depression after stroke. Methods : 173 patients with cerebral infarction whose Barthel’s index improved over 90 points were included. Beck Depression Inventory (BDI) was used to evaluate depression, and the patients were divided into two: depression (DG) and non-depression (NDG) groups according to their BDI score (cut off=21). We then analyzed factors, including plasma homocysteine and MTHFR genotype. Results : The DG was 49 patients (28.3%) and the NDG were 124 patients (71.7%). The genotype distribution consisted of 22.4%(CC), 57.2%(CT), 20.4%(TT) in DG and 27.4%(CC), 55.5%(CT), 16.1%(TT) in NDG, the frequency of which was not different from that in DG. Age, sex, lesion location (left/right/bilateral , anterior/posterior/lenticulostriatal/ multiple), an interval from the stroke onset to the evaluation, the presence of diabetes mellitus, hypertension, hyperlipidemia, cardiac disease, and family history were not different between the two groups. The plasma homocysteine level was significantly higher in DG (14.70 mmol/L) than NDG (11.51 mmol/L) (OR 1.094;95% CI 1.013-1.180) after controlling of the factors described above. Conclusions : Our results suggest that homocysteine may play a role in the pathogenesis of post-stroke depression and support a vascular depression theory. Early identification of this risk factor may lead to effective therapeutic intervention.

충주문경간(忠州聞慶間)의 옥천계(沃川系)의 층서(層序)와 구조(構造)

김옥준,Kim, Oku Joon 대한자원환경지질학회 1968 자원환경지질 Vol.1 No.1

Some of geologists in Korea recently postlated that Okchon system previously known to be precambrian age was the metamorphosed sediments of post-Chosen (Ordovician and pre-Kyeongsang (late Jurassic to Cretaceous) periods, or even definitely of Triassic period simply on the basis of the fact that Okcheon system overlies the Great Limestone series of Chosen system of Camber-ordovician age, and of other few assumptions of minor importance. As a result of such correlation, thick series of metasediments and Okcheon system of unknown age were established in this particular region and vaguely correlated to Paleozoic and Mesozoic sediments. Recent study done by the author reveled that: 1) only the upper Okcheon bed of S. Nakamura was true Okcheon system, and the middle and lower Okcheon beds were excluded, because they were correlated to Cambrian and Permian sediments resfectively, 2) Sangnaeri, Seochangri, and rengam formations of unknown age, and Baekhwasan, Jobong, and Ihwaryeong formations of Okcheon system of also unknown age were the metamorphosed Yangdeok system of Cambrian age, all of these formations were differentiated by the previous workers and were equivalent to the middle Okcheon system of S. Nakamure, and. 3) These metamorphosed Yangdeok system overlaid apparently the Great Limestone series in forms of overthrust and klippe which were produced by the orogeny took place during post-Daedong and pre-Kyeongsang period (probably middle to the Jurassic). The Sobaeksan Range, folded mountain Chains was also formed by this orogeny. Thus, Okcheon system newly defined by the author is precambrain age and consists in ascending order of Kemyenogsan, Hyangsan dolomite, and Daehangsan quartzite formation which were previously classified into metasediments of unknown age, and Munjuri, and Hwangkanri, formations which were differentiated into Okcheon system unknown age by the previous workers, but are of reversed sequence. Myeongori and Bukrori formations of Okcheon System are regard by the author as part of Hwangkanri formation. Few other assumption of minor important taken by the previous workers as their positive evidences are carefully explained that they were misinterpreted.

Differential Expression of Extracellular Matrix-Associated Genes Between Dermal and Gingival Fibroblasts in Response to Arachidonic Acid

김옥준,임원봉,장미선,김지선,고영종,김인애,권혁일,김상우,최홍란 대한구강악안면병리학회 2010 대한구강악안면병리학회지 Vol.34 No.2

The pattern of wound healing process differs markedly according to the cell types. Gingival wounds heal more rapidly without scar, however dermal wounds show collagen laid down in thick disorganized patterns and keloid formation. This h as b een s uggested t o be d ue t o the presence of d ifferent E C M components a nd c ytokines a s well a s growth factors. The purpose of this study was to examine the differential expression of genes in connection with keloid formation in gingival fibroblasts (hGFs) and dermal fibroblasts (hDFs) in response to inflammation. In this study, we investigated the differences between hGFs and hDFs in the expression and production of cyclooxygenase (COX-2), prostaglandins E2 (PGE2), transforming growth factor (TGF)-β, collagens, matrix metalloproteinases (MMPs), and tissue inhibitors of matrix metalloproteinases (TIMPs) which play important roles in collagen deposition in wound healing. The hGFs and hDFs were primary cultured and allocated to arachidonic acid (AA) treatment group and control group. Protein and mRNA were extracted right after (0 hr) and 24 hr after AA treatment. At a defined concentration of AA in hGFs and hDFs, MTT assay was performed. The mRNA and protein expression levels of COX-2, TGF-β, collagen 1and 3, MMP 1 and TIMP 1 were examined by Real-time PCR and Western blots. The amounts of PGE2 were measured by enzyme-linked immunosorbent assay (ELISA).The expression of COX-2 and TGF-β exhibited reduced levels in hGFs , but were increased in hDFs at 24 hr after AA treatment. Production of PGE2 was increased in hGFs and hDFs at right after AA treatment but, not changed at 24 hr after AA treatment. The protein and mRNA expression of collagen 1 and 3 were decreased in hGFs , whereas increased in hDFs at 24 hr AA treatment. Expression of MMP-1 protein was increased in hGFs at 24 hr but, was decreased in hDFs at 24 hr compared with that of control. The protein expression of TIMP-1 was decreased in hGFs but, was increased in hDFs at 24 hr compared with that of control. These observations demonstrate differential expression between gingival and dermal fibroblasts in regulation of collagenolytic capacity by extracellular matrix-associated genes in keloid formation associated with wound repair.

응급센터 체류시간 단축을 위한 6-sigma 활동

김옥준,김재화,박민정,이광호,이혜경,김현식,이창주,최상문,신현수 한국의료QA학회 2007 가을학술대회 Vol.2007 No.3

수산리(水山里)-황강리지역(黃江里地域)의 지질구조(地質構造)와 암석학적(岩石學的) 연구(硏究)

김옥준,김규한,Kim, Ok Joon,Kim, Kyu Han 대한자원환경지질학회 1974 자원환경지질 Vol.7 No.3

The study area is located in between Susanri and Hwanggangri where the formations of Okcheon group and Chosun group supposedly come in contact so that the area is structurally very import. Present study reveals that the meta-volcanic rocks distribute from south to north along contact zone of Okcheon and Chosun groups in the center of the area. Meta-volcanic rocks seem to be originated from the andesite or andesitic basalt rocks which was known to be Surchangri formation consist of phyllite and black slate by previous workers. The meta-volcanic rocks intruded along the fault zone one existed between Okcheon and Chosun groups but obliterated at present by the intrusion of volcanic rocks. The fault seems to be overthrust, and one of the positive evidences of thrust fault is the Yamisan nappe structure in Yamisan near Susanri. This interpretation coincides with O.J. Kim's work which states that the Precambrian Okcheon group is largely overturned and thrusted over the Chosun group. The relation between the Surchangri and the Majeonri formation marks facies change. This fact together with northpluging anticlinal structure made it possible that both formation came into contact along direction without fault. Yongam formation is not overlain unconformably used to be believed by previous workers, but interbed in the Great Limestone series of Chosun goup. It is also clarified that the rock formerly designated as limesilicate rock was meta-liparite. The origin of amphibole pebbles in the Kunjasan formation is of primary and secondary ones; the secondary pebbles were formed by metamorphism of the fragments of limestone or dolomite.