Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

농장 사육되는 타조에서 나타난 다리 기형의 증례 보고

최종윤,손화영,전무형,조성환,김혜성,류시윤,박배근,이영원,윤원기 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

Two farmed young ostrich chicks were applied to pathology laboratory at Chungnam National University. Grossly, one ostrich showed rotated tibiotarsus, the other showed rolled toes. Case 1 ostrich was hypertrophy of fibualis longus and tibial cranialis tendon and gastrocnemius tendon in crus, inflammation of flexor digitorum longus. In histopathological views, myocytes of fiburalis longus were showed hypertrophy and hyperplasia. It is not found any specific feature in X-ray of the case 2 ostrich. Limb deformities were detected frequently in farmed ostrich chicks in 2- to 3 weeks old. Limb deformities were an important cause of mortality in farmed ostrich chicks during the first 10weeks after hatch. Cause of limb deformities were considered a nutrition problem.

運動種目別로 본 PWC_(170)時에 發現되는 運動能力에 관한 比較硏究

최영근,김상국,김정주 경희대학교 체육대학 한국체육과학연구소 1984 體育學論文集 Vol.12 No.-

With two Gymnastics players, three Basketball players, four Baseball players, four Football players as the Subjects, this study was made in order to reveal the degree of Physical Work Capacity (PWC) 170 by measuring how Heart Rate would change when they run on the Treadmill. The Results are as following: 1. In max Heart Rate, Baseball players were the highest as 209.00±4.90 beats/min, Gymnastics players were 195.50±3.54 beats/min, Football players were 192.00 ± 7.02 beats/min and Basketball players were the lowest as 187.00 ± 8.89 beats/min. 2. In max Running time and Running distance, Football players were the highest, 11.66 ± 0.59 minutes as Running time, 2450.3 ± 151.28m as Running distance, Basketball players were 11.21 ± 1.73 minutes and 2358.9 ± 381.77m, Baseball players were 10.69 ± 0.47 minutes and 2256.9 ± 104.60m and Gymnastics Players were the lowest, 8.50 ± 0.03 minutes and 1721.80 ± 9.90m. 3. In Heart Rate increase while they run on Treadmill, it was totally the highest in Baseball players, and was appeared orderly in Gymnastics players, in Football players, and in Basketball players. % HR max was appeared generally that Gymnastics players were appeared the highest, and was appeared orderly Baseball players, Basketball players, Football players. 4. In Heart Rate change while the Recovery, the Basketball players were appeared generally low Heart Rate at the Sometime, and was appeared orderly Football players, Gymnastics players, Baseball players. % H.R. max, Basketball players was recovered the fastest speed. Within 4min recovery it was appeared quickly that was orderly Basketball players, Baseball players, and after 4min was appeared orderly Basketball players, Gymnastics players, Baseball players, Football players. 5. In PWC 170 test, Basketball players were appeared superior that 9.09 min in Running time and 1781.0m in Running distance, and 240m/min in load and 6% in slope. And next Football players were appeared 7.27 min in Running time, 1463.4m in Running distance, 220m/min in load, and 5% in slope, Gymnastics players were appeared 5.53 min in Running time, 968.0m in Running distance, 200m/min in load and 4% in slope. Baseball players were appeared inferior that was 12 min in Running time, 704.0m in Running distance, 200m/min in load, and 4% in slope.

여드름의 원인과 치료

최광성,김영근 한국의학사 1999 診斷과治療 Vol.19 No.8

돼지 신장세포(PK15)의 이종이식 거부반응에 대한 재조합 IL-18의 영향

최연실,김은미,김영관,박상연,심정현,윤도영,강형식,명평근 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

Various cytokines including IL-1β, IL-2, IL-4, IL-10, IL-12p40, IFN-r, and TNF-α have been recently reported to play important roles in both host and donor cell activations during xenotransplantation. Interleukin (IL)-18, a proinflammatory cytokine, can induce IL-4 and IL-13 production in T cells, NK cells, B cells, mast cells, and basophils. IL-18 has the capacity to stimulate innate immunity and both Th1-and Th2- mediated responses, but little is known to involve in the xenograft rejection. In order to investigate the role of IL-18 in xenograft rejection, we transplanted the pig kidney (PK15) cells to C57BL/6 mouse with or without intraperitoneal injection of mouse recombinatant IL-18, It was analyzed the population of T cell, B cell and NK cell in the mice transplanted with PK 15 cells and recombinant IL-18 by flow cytometry. We found that splenic CD3+ T cells were increased in mice injected PKl5 cells with recombinant IL-18. These results suggest that recombinant IL-18 is critical in the xenograft rejection by increasing T cell population of the recipients.

fac-Tris(L-serinato)chromium(Ⅲ) 화합물의 분광학적 연구 및 리간드장 분석

최영근,서정선,이규왕 명지대학교 자연과학연구소 1995 자연과학논문집 Vol.12 No.-

중심금속chromium(III)와 리간드인.amino acid(L-serine)[serine=HOCH₂CH(NH₂)CO₂H]사이의 결합의 특성을 파악하기 위하여 저온(13K)에서의 luminescence 스펙트럼과 excitation 스펙트럼을 측정하였다.Tris (L-serinato)chromium(III)착화합물은 산소 원자 3개와 질소원자 3개로 이루어진 facial 형태의 geometry를 가진 착화합물이다.Amino acid 를 리간드로 가진 다른 착화합물과는 달리, serine 착화합물은 β탄소위치에 hydroxy group이 치환되어 있어서 electronic effect 와 steric dffect의 효과를 기대할 수 있다. 산소원자의 π비등방성 결합을 포함한 ligand field analysis로 부터, serine 착화합물의 자세한 electronic structure와 결합특성을 살펴보기로 하겠다. Low temperature (13K)luminescence and excitation spectra were measured to anlayze the bonding characteristics between Cr(III)and amino acid(L-serne) ligand. Tris(L-serinato)chromium(III) complex has three oxygen and three nitrogen ligand atoms in facial geometry. Unlike oter complexes with amino acids, additional hydroxyl group in β-carbon tom is expected to have electronic an steric effects. From the ligand field analysis including anisotropic πbonding on oxygen atom, we found that both oxygen and nitrogen atom is weak σdonor. The splitting of electronic lines and bonding characteristics in the title compound are explained by considering the geometric effect, especially anisotropic πbonding.

인천 인현동 호프집 화재 피해자 분석

최정태,안무업,안희철,최영미,정재봉,서정열,유기철,이삼우,박석현,조준휘,김성환,김아진,송근정 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Purpose : This study was conducted to develop field triage, transportation, distribution, and prehospital care at a fire disaster by analyzing the victims of the fore that broke out at a bar in Incheon. Method : We analyzed the cases of the victims of a fire in Incheon in Oct. 1999. We determined the primary care hospital, the arrival time, the burn size, the outcome, and the injury type from the medical records, the concerned organ records, and interviews with concerned persons. Result : The total number of victims was 137: 56 prehospital deaths, 1 hospital death, and 80 survivals. The Pearson correlation coefficient between the burn size and the severity was -0.175. There were 121(89.6%) cases of inhalation injury, 59 (43.7%) cases of flame burns, 66 (48.9%) cases of hypoxic brain damage, and 16 (11.9%) cases involving other types of injury. Conclusion : The causes of death of the fire victims were inhalation injury and hypoxic brain damage due to CO poisoning and other toxic inhalants. We propose the use of a simple triage and rapid treatment(START) system and a reassessment the delayed category in fire disasters.

Videodensitometer를 이용한 치은연하소파술후 치간골 골밀도의 변화

최진근,이만섭,권영혁 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.1

The purpose of this study was to evaluate the changes of interproximal bone density by means of videodensitometer and to examine the clinical applicability of videodentitometer to assess the periodontal disease activity: Twelve interproximal sites, with periodontal pockets deeper than 5mm and vertical Loss of bone on standard dental radiograph, were treated by subgingival curettage. The papilla bleeding index, the plaque index, the degree of mobility, the depth of pockets, and the level of attachment were measured. Standardized reproducible radiographs were taken by using the occlusal stent with parallelling film holder. The density of the interdental bone was measured on the radiographs by a videodensitometer at three levels : the most 'superficial' level; the 'deep' level, arbitrarily 1.5mm below; and the `apical' level, where no bony changes were to be expected. The clinical parameter and the radiographical change were measured at initial, and 1 month, 3 months, and 6 months after treatment. The results were as follows 1. The papilla bleeding index and the degree of mobility decreased significantly until 3 months after subgingival curettage and showed the same level in the remaining experimental periods. 2. The pocket depth mainly decreased due to the gingival recession until 1 month after treatment, but to the attachment gain after 1 month. 3. The density of the interdental bone did not show a significance increase until 1 month after treatment, but showed a steady increase throughout the 6 months of observation. 4. The close relationships were shown between the decrease in pocket depth and the gain of attachment and the improvement of bone density at 6 months after treatment.