전주산업단지 및 주변지역 VOCs 및 악취물질 모니터링

김용문,박비오,고영삼,심재정,송병식,양고수 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2002 工學硏究 Vol.33 No.-

Important main odorous compounds and VOCs(volatile organic compounds) possibly emitted from the Jeon-Ju Industrial Sites were identified and measured in amount through making air sampling over Jeon-Ju Industrial Sites and analyzing them with the sate of the art analytical instrument. Sampling sites were chosen 8 points by considering the industrial characteristics for the 1,2 Industrial Sites and 2 points for the 3 Industrial. Site Sampling times were two times, the first one was March. 2002 - April. 2002, and the second was May. 2002 - July. 2002. Canister sampling units and polyester bags were used to collect 6 liters air samples during 1hour or 3minutes for VOCs and polyester bags were used to sample 10 - 50 liters of air for 3 minutes depending on analytic characteristics of odor compounds. The important main VOCs were measured as acetone, ethyl benzene, n-hexane, m,p-xylene, toluene, M.E.K.(methyl ethyl ketone), isopropanol, ethyl acetate, o-xylene, n-butyl acetate, ethanol, benzene as in order of average concentration for the Industrial Sites. The main odorous compounds were COS, H2S, CS2, MM(methyl mercaptan), form aldehyde, acetaldehyde for the 1,2 Industrial Sites and COS, H2S, form aldehyde, acetaldehyde were measured as main species for the 3 Industrial Site. NH3 was measured as below the detection limit of the instrument. The concentrations of sulfide compounds for the 1,2 Industrial Sites were relatively higher than ones for the 3 Industrial Site. Among aldehydes, form aldehyde, acetaldehyde were detected very highly as 0.1- 1ppm over all the Industrial Sites.

미만성 대형 B-세포 림프종에서의 예후인자에 관한 연구

김삼용,곽승근,박상은,윤환중,조덕연,김진만 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

미만성 대형 B-세포 림프종은 가장 흔한 악성 림프종의 한 종류로서 우리나라에서는 전체 비호지킨 림프종의 50%를 차지한다. 비호지킨 림프종에서 병기는 호지킨병과는 달리 예후 인자로서의 유용성이 적다. 따라서 병기만을 예후 인자로서 평가하는 한계를 극복하기 위해 국제예후지표(International Prognostic Index)가 도입되었다. 특정 환자에서 IPI 점수와 함께 그 환자의 림프종의 생물학적 특성을 분석한다면 예후 예측에서 정확도가 높아질 것이다. 본 연구에서는 미만성 대형 B-세포 림프종에서 국제예후지표의 유용성을 확인하고자 하였으며, 각종 생물학적 지표의 면역화학염색을 통하여 이들을 배세포 중심 아형(germinal center subgroup : 이하 GC양 아형으로 약함)과 활성화 B-세포 아형(post-germinal center subgroup; 이후 pGC양 아형으로 약함)으로 분류하고 이들의 예후 예측에 대한 적절성을 평가하였다. 결과는 다음과 같다. 1. 연령분포는 37세에서 69세로 중앙치는 56세이었으며, 남자는 17예(65.4%), 여자가 9예(34.6%)였다. 병기는 Ⅰ, Ⅱ, Ⅲ, Ⅳ기가 각각 2예(7.7%), 11예 (42.3%), 5예(19.2%), 8예(30.8%)였다. 'B'증상은 5예(19.2%)에서 양성이었다. 진단 당시 혈청 LDH치가 상승한 경우가 11예(42.3%)였고, 거대종양(직경이 10㎝이상)을 갖는 경우가 5예(19.2%)이었다. ECOG 수행등급은 0이 2예(7.7%), 1이 18예(69.2%), 2가 5예(19.2%), 3이 1예(3.8%)이었다. 2. 면역조직화학염색 상 bcl-2의 양성은 17예(65.4%), bcl-6의 양성이 20예(76.9%)이었다. CD10이 양성인 경우가 5예(19.2%)이었다. IRF-4가 양성인 경우는 8예(30.8%)였다. 26명의 환자에서 bcl-6, CD10과 IRF-4를 이용하여 GC양 아형과 pGC양 아형으로 분류 하였는데, GC양 아형은 14예(53.8%)이었고, pGC양 아형은 7예(26.9%), 분류가 되지 않는 경우가 5예(19.3%)이었다. 3. 평균 48개월 동안 추적한 결과 중앙생존기간은 44개월이었고 생존기간은 1~100+개월에 분포하였다. Kaplan-Meier법에 의한 5년 누적 생존률은 32%이었다. 4. 병기, 'B' 증상, 혈청 LDH 농도, ECOG 수행등급, 거대종양유무가 통계학적으로 의미있는 임상적 예후 인자였다. 5. 면연조직화학염색에서는 bcl-2만이 통계학적으로 유의한 예후인자였다. 6. GC양 아형은 pGC양 아형에 비해 생존기간은 좋았으나, 통계학적 유의성은 없었다. 7. 다변량 분석결과 bcl-2 발현과 혈청 LDH치만이 예후에 영향을 미쳤다. Clinical Significanceof Expression of Biomarkers in Patients with Diffuse Large B-cell Lymphoma Diffuse large B-cell lymphoma(DLBL) is the most common type of lymphoma in Korea, representing about 50% of non-Hodgkin's Lymphomas. Although DLBL is usually considered as a specific category, the diversity in clinical presentation, morphology, genetic and molecular alterations strongly suggest that these tumors represent a heterogeneous group of neoplasia rather than a single clinicopathological entity. Clinical prognostic systems, including the International Prognostic Index (IPI), although useful to assess overall prognosis, embraces patients with heterogeneous prognoses. It is likely that the prognostic assessment of patients with DLBL might be improved by using biological features. During the last decade, most studies dealing with the heterogeneity of DLBL have focused on individual protein expression and molecular alterations. The expression of individual antigens related to different stages of B-cell differentiation, including CD10, bcl-6, and IRF-4, may help to define groups of tumors with different clinical and pathological characteristics. To determine the clinical significance and prognostic value of individual biomarker expression and the biomarker expression patterns according to germinal center subgroup or post-germinal center subgroup of DLBL as defined by a relatively small number of single antigens, we studied 26 patients with de novo DLBL, whose archival pathology specimen were available for immunohistochemistry studies, atChungnam National University Hospital from September 1992 to December 2000. Archival specimens from each patient were immunostained with respective antibodies for CD10, bcl-6, IRF-4, bcl-2 antigens. Two immunophenotyping profiles were distinguished according to the pattern of differentiation; germinal center(GC;CD10/Bcl-6+/IRF-4-) subgroup or postgerminal center (pGC ; CD10/bcl-6/IRF4+) subgroup. The results are as follows; 1) Baseline characteristics of patients were ; median age of patients was 56(range ; 37-69). 17 patients(65.4%) were male. 5 patients(l9.2%) had 'B' symptoms. Stages were as follows ; stage Ⅰ2 patients(7.7%), stage Ⅱ 11 patients(42.3%), stage Ⅲ 5 patients(l9.2%), stage Ⅳ 8 patients(30.8%). 11 patients (42.3%) had elevated LDH levels. 25 patients(96.2%) had ECOG performance grade 0-Ⅱ and 1 patient had grade Ⅲ. 5 patients(l9.2%) bad bulky disease. 2) The results of immunohistochemical study were as follows ; positive bcl-2 staining in 17 patients(65.4%), positive bcl-6 in 20 patients(76.1%), positive CD10 in 5 patients(l9.2%), positive IRF-4 staining in 8 patients (30.8%). 3) After a median follow up duration of 48 months, the median survival duration was 44 months with a range of survival of 1-100+ months. 5-year overall survival rate was 32% by Kaplan-Meier method. 4) The clinical factors affecting survival were elevated serum LDH level, B symptoms, bulky disease, stage at diagnosis and ECOG performance status 5) Among the biomarkers, only bcl-2 expression affected survival of DLBL patients 6) The germinal center like subgroup had superior survival than post-germinal center like subgroup but had no statistical significance. 7) Multivariate analysis showed that bcl-2 and serum LDH level had siginificance on survival Conclusion In addition to the known clinical prognostic factors, immunohistochemically defined characteristics such as bcl-2 expressionin DLBL is important and subgroups(germinal center or post-germinal center subgroup) of DLBL may have importance in predicting prognosis of DLBL patients

다발성 연조직 종괴를 동반한 급성 림프구성 백혈병의 증례 보고

장정용,허경회,이원진,허민석,이삼선,최순철 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.2

A 15-year-old patient, who had been diagnosed and treated as Burkitt cell type acute lymphoblastic leukemia (ALLL3) already, visited our department. He complained of gingival enlargement and loosening teeth 1 month ago. The clinical examination revealed anterior open bite, gingival enlargement, and nontender swelling particularly in molar regions of both jaws. Deep periodontal pockets and severe mobility was shown on most of the teeth. The panoramic radiographs showed severe bone destruction and extrusion of the molars. The contrast enhanced CT showed multiple enhanced mass and bone marrow obliteration in both jaws. Chemotherapy was done and the swelling was subsided at 1 month later. In conclusion, radiologic findings of leukemia with soft tissue mass, known as chloroma or granulocytic sarcoma, mimic those of lymphoma, so blood test may be needed for the final diagnosis.

림프절 종창의 임상적 관찰

김삼용,김현수,김종숙,박상준,최지영,윤환중,조덕연 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

Objectives : Clinical management of patient with lymphadenopathy requires judicious judgement of clinicians. Careful evaluation of lymph node enlargement by various clinical parameters will help to disclose the etiology of lymph node enlargement. This study was conducted to identify the clinical manifestations and etiology of lymphadenopathy in korean patients. Methods : A total 192 patients with lymph node enlargement were studied retrospectively. All patients who underwent excisional lymph node biopsy during the period from January 1992 to July 1994 were included. The lymph node biopsy and clinical records were reviewed and patient characteristics were analyzed by various clinical parameters. Results : A female predominance was noted in lymphadenopathy of tuberculosis (58.5%), Kikuchi's syndrome (70%). In lymphadenopathy of nonspecific, tuberculosis, kikuchi syndrome, per centage of patients younger than 40 years of age were 84.5%, 75.6%, 100%, respectively. In lymphadenopathy of tuberculosis and metastatic cancer, the duration of lymphadenopathy below 30days were 51.2%, 73.9%, respectively. The two most common etiology of cervical lymphadenopathy were nonspecific lymphadenitis (47.7%) and tuberculous (26.8%). The two most frequent etiology of supraclavicular lymphadenopathy were nonspecific lymphadenitis (46.3%) and metastatic lymphadenopathy (26.8%) and the most common etiology of axillary lymphadenopathy was nonspecific lymphadenopathy (34.5%). The most common frequent of lymph node enlargement with size below 1㎠ was nonspecific lymphadenitis, while above 2.25㎠ it was metastatic lymphadenopathy. Conclusion : Careful evaluation regarding patient age, sex, size of lymph node, consistency, location of lymph node, presence or absence of systemic symptoms should be pursued before performing a biopsy and management.

Luis Barragan의 후기건축에 나타난 지역주의 표현경향에 관한 연구

이용재,이정훈,김희규,문영삼 순천향대학교 교수학습개발센터 2005 순천향 산업기술연구소논문집 Vol.11 No.1

The purpose of this study is to define the architectural expression of regionalism in the late period of Luis Barragan, the representative architect of Modernism in Mexico. The view point of architectural expression, the essential quality of regionalism be related to the sensitivity, passionate, and purity. We select the works of L. Barragan in the late period : Barragan House(Tacubaya, 1947), Chapel for the Capuchinas(Tlalpan, 1952) and Gilardi House(Tacubaya, 1976). The result of this study shows that the architectural expression of regionalism on the his works in the late period are the passionate sensitivity, regional commonness and human being experience in Mexico.

냉동 제대혈 세포의 체외 증폭

김삼용,김철희,배광봉,김현수,박상준,김종숙,윤환중,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Background : Cord blood(CB), which has no HLA restriction, is an alternative to bone marrow for hematopoietic stem cell transplantation. The use of cord blood, however, is limited by the number of progenitor/stem cells necessary to reconstitute the older child or adult. Therefore, ex vivo expansion of CB could have tremendous impact on diverse clinical settings. We studied the ex vivo expansion of isolated population of CD34_(+) cells from cryopreserved CB cells. Methods : CD34 cells were isolated from cryopreserved CB mononuclear cells. Purified cells were cultured with various combinations of hematopoietic growth factors including erythropoietin(EPO), stem cell factor(SCF), granulocyte-colony-stimulating factor(G-CSF), gra-nulocyte, macrophage-colony-stimulating factor(GM-CSF), interleukin-1β(IL-1β), 1L-3, and IL-6. After 7, 10 or 14 days of culture, the fold increases of colony-forming unit- granu-locyte, macrophage(CFU-GM), burst-forming unit-erythroid(BFU-E), colony-forming unit-mix (CFU-Mix), and high proliferative potential colony-forming cell(HPP-CFC) were evaluated. Results : Ten-day culture with the combination of EPO, SCF, G-CSF, IL-1β, and IL-3 resulted in a median of 60-fold increase of CFU-GM, which was greater than those with the combinations of less than 5 growth factors. The addition of IL-6 or GM-CSF to this combination did not enhance CFU-GM expansion. Ten-day culture was significantly superior to 7-day culture for CFU-GM expansion. Prolongation of culture to 14 days, however, revealed decreased expansion of CFU-GM compared to 10 days. BFU-E and CFU-Mix were expanded to 2~5 folds in 7-day culture with the combination of EPO, SCF, and G-CSF. Further expansion was not achieved in 10-day culture and colonies disappeared in 14-day culture. HPP-CFC was expanded to a median of 7.5 folds in 7-day culture with the combination of EPO, SCF, G-CSF, IL-1β, IL-3, and IL-6. Neither 10-day or 14 day-culture enhanced expansion of HPP-CFU. Conclusion : Cryopreserved cord blood cells maintain ex vivo expansion potential. In our system, 10-day culture with the combination consisting of EPO, SCF, G-CSF, IL-1β, and IL-3 seems to be adequate for hematopoietic progenitor/stem cell expansion from cryopreserved cord blood cells.

혈관중심성 T세포/자연살상세포 림프종의 임상적 고찰

이정찬,박수진,박상은,곽승근,신현영,김성은,이정호,윤환중,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Although the Working Formulation is commonly used to classify NHL in Korea, it has been recognized as imperfect for primary extranodal lymphoma, expecially for patients with nasal, paranasal disease because of their histological characteristics. Angiocentric T/NK cell lymphoma remains rare clinical presentation in North America and Europe but is more common in Asia and Latin America. The optimal mode of treatment has also not been decided on, probably because of their rarity and limited understanding of its natural course. The aim of this study was to analyze our experience with 14 patients with angiocentric lymphoma seen at the Chungnam Natioanl University Hospital over the last 5 years. We reviewed the records of 14 patients who were treated at the Chungnam National University Hospital between January, 1996 and December, 2000. The angiocentric T/NK cell lymphoma accounted for about 8.2% of NHL. The median age was 52.5 (range 36-71) years. Sex ratio was equal. According to the Ann Arbor staging system, disease was classified as stage Ⅰin 3 patients, stage Ⅱ in 8 patients and stage Ⅳ in 3 patients. Systemic B symptoms were present in only four patients in only four patients. All patients has extranodal involvement. The most frequently involved extranodal sites were nasal cavity followed by paranasal sinuses and orpharynx. The most common presenting symptoms were nasal obstruction with purupent rhinorrhea followed by dysphagia. According to the International prognostic index (IPI), most patients was in low-risk group (11 patients). Nine patients responded completely. Seven patients were received combination chemotherapy and 2 patients had radiation therapy as a initial treatment. Five patients were relapsed. After a median follow-up of 660.5 days, the overall survival and disease-free survival rates at 3 years was 56.8% and 42.9% respectively. The median survival time was not yet reached. The median overall survival time was 1338.5 (range 1212-1564) days for non-relapsed and 733.4 (range 302-1008) days for relapsed patients. The median time from CR to relapse was 100.5 (range, 39-159) days. There were limitations to know enough about the characteristics of angiocentric T/NK cell lymphoma because of a small number of patients. In the future, multicenter trials will be neccesary to identify clinical characteristics, optimal treatment mordalities and their responses, and prognostic factors.

가막만에 분포하는 대형저서동물의 계절적 분포 및 출현

최상덕,문성용,안치영,윤호섭,정형택,이삼노,서호영 國立 麗水大學校 환경문제硏究所 2001 環境硏究論文集 Vol.4 No.-

This study was coducted to describe the distribution and appearance pattern of macrobenthos in Kamak Bay, the southern coastal of Korea. Sampling was perfound seasonally using Van Veen grab(0.1㎡) from April 2001 to December 2001. A total of 22 macrobenthic species occured: Bivalvia accounted for 12 species(55%), and Mollusca 5 species(23%). The mean density of macrobenthos was 153.9ind./㎡, a major dominant species were a Bivalvia, Paphia undulata(28.3ind./㎡), and a Cirripedia, Chthamalus challengeri(5ind./㎡), which frequency occur in the costral area. These species showed very low density at station 1 in the Kamak Bay.

酸化鐵의 缺乏構造에 關한 硏究

片茂實,姜參龍,徐廷善 명지대학교 1972 明大論文集 Vol.5 No.-

The defect form of the iron oxide system is studied by analyzing the gravimetric analysis of a sample measured by using a quartz microbalance(in a temperature range from O℃ to 1,200℃ under oxygen Pressures from 10^2mmHg to 10^-4 mmHg The Y Values of the formula FeO_1+y, are corretly obtained by this means for various condition of temp erature and pressure The plots of log Y v, s log P0_2 (or log Y=1/nlog Po_2) show linearity and calculates from the slope of the plot is about 1/10 at 1,000℃ The most of physical properties of iron oxide system such as electrical conductivity oxidation mechanism and catalytic effect is also discussed with respect to the defect form or the Y values.

항암제 처리한 백혈병 세포주에서의 Apoptosis 발현

김삼용,윤소현,김현수,김종숙,윤환중,김진경,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

The bcl-2 proto-oncogene encodes a 26 kD protein that promotes cell survival by blocking apoptosis. The bax protein is a member of the bcl-2 familly, now known to form heterodimers with the bcl-2 protein. The ratio of bax to bcl-2 is be critical in determining the fate of the cell in response to stimuli that can induce apoptosis. Extract of Pulsatilla Koreana (SB-31) showed promising antitumor activity in vitro with Topo I inhibitory action. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax were investigated in human leukemic cell lines HL-60, U-937 and CEM-CM3. All anticancer drugs(adriamycin, etoposide, camptothecin, SB-31) induced extensive apoptosis in HL-60, U-937 cells and CEM-CM3 cells. The expression of bcl-2 and bax protein were determined in cell lines by western blotting before and after incubation with anticancer drugs at different time points. 1) In HL-60 or U-937 cell lines, down regulation of bcl-2 and up-regulation of bax were found after incubation with ADR, VP-16 or camptothecin. 2) In HL-60 or U-937 cell lines, no significant change in bcl-2 or bax protein expression resulted after incubation with SB-31. 3) In CEM-CM3 cells, virtually no change was noted in bcl-2/bax expression after incubation with ADR, VP-16, camptothecin or SB-31. It is suggested that different leukemic cell lines use different pathways of apoptosis activation and a given cell may utilize different pathways of apoptosis activation in response to different cytotoxic agents.