Identification and expression patterns of 20-hydroxyecdysoneresponsive genes from Procambarus clarkii

Bao‑Jian Zhu,Lin Tang,Ying‑Ying Yu,Dao‑Jun Wang,Chao‑Liang Liu 한국유전학회 2017 Genes & Genomics Vol.39 No.6

Ecdysteroids play an important role in the regulation of molting process in crustaceans. The suppression subtractive hybridization (SSH) technique was employed to search for 20-hydroxyecdysone-responsive genes in the hepatopancreas of the crayfish Procambarus clarkii. Ninety-three putative expressed sequence tags were identified by SSH, including three immune response-related genes, two cell cycle and apoptosis genes, four respiration and energy metabolism genes, four transport-related genes, six metabolism-related genes, two stress response genes, and eight transcription and translation regulation genes. The expression levels of the examined genes were regulated by 20-hydroxyecdysone and varied in the hepatopancreas during the molting stages; data were confirmed by real-time PCR. RNAi of the ecdysteroid receptor had significant effects on the expression levels of 20-hydroxyecdysone- responsive genes. These differentially expressed genes identified by SSH will provide insight into the ecdysteroid signaling pathway in P. clarkii.

Genetic Diversity and Evolutionary Relationships of Oryza Species with the B- and C-Genomes as Revealed by SSR Markers

( Ying Bao ),( Hai Fei Zhou ),( De Yuan Hong ),( Song Ge ) 한국식물학회 2006 Journal of Plant Biology Vol.49 No.5

內蒙古牧區鄂溫克族婚姻家庭與民族認同

( Ying Hua Bao ) 전북대학교 인문학연구소 2013 건지인문학 Vol.10 No.-

Based on the previous research and the investigative data avail-able in the pasture, the paper expounds that the marriage and fam-ily of the Ewenki ethnic group are tending to the sense of national identity, and delineates that the distinction of the Ewenki ethnic group is gradually confused because of the overwhelming trends of modernization, in addition, socialization has changed the mode of ethnic group identity obtained through marriage and family relations.

Baicalein Induces Programmed Cell Death in Candida albicans

Bao Di Dai,Ying Ying Cao,Shan Huang,Yong Gang Xu,Ping Hui Gao,Yan Wang,Yuan Ying Jiang 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.8

Effects of Hovenia species on memory and neurotransmitter levels in ethanol-treated mice

Bao Hai Ying,Chen Quan Cheng,Zhang Jing,Kim Young Ho,Cho Jungsook,Kang Jong seong 대한약학회 2004 大韓藥學會 總會 및 學術大會 Vol.2004 No.1

Extensive reprogramming of cytosine methylation in Oryza allotetraploids

Ying Bao,Qing Xu 한국유전학회 2015 Genes & Genomics Vol.37 No.6

Cytosine methylation is a prominent epigenetic modification in plants and it has been proposed to as causative for silencing some duplicates derived by polyploidization. Several synthetic polyploids have shown there is a dynamic epigenetic change following hybridization and genome doubling. Relatively few studies, however, have focused on wild natural polyploids. In this study, using a methylation-sensitive amplified fragment length polymorphism method, we investigated the levels and types of cytosine methylation in three Oryza CCDD genome allotetraploids and their related diploids with CC and EE genomes. We show an overall minor increase in methylation level in the allopolyploids, but extensive methylation reprogramming (70 %), with almost equivalently increased (22–26 %) and decreased methylation (25–27 %). Our study reveals a complicated epigenetic change in natural polyploids. The information presented in the study will be of some utility to molecular taxonomists and also rice breeders working on wide-hybridization.

Memory Enhancing and Neuroprotective Effects of Selected Ginsenosides

Hai Ying Bao,Jing Zhang,여수정,Chang-Seon Myung,Hyang Mi Kim,Jong Moon Kim,박정일,조정숙,Jong Seong Kang 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.3

The effects of ginsenosides Rg3(R), Rg3(S) and Rg5/Rk1 (a mixture of Rg5 and Rk1, 1:1, w/w), which are components isolated from processed Panax ginseng C.A. Meyer (Araliaceae), on memory dysfunction were examined in mice using a passive avoidance test. The ginsenosides Rg3(R), Rg3(S) or Rg5/Rk1, when orally administered for 4 days, significantly ameliorated the memory impairment induced by the single oral administration of ethanol. The memory impairment induced by the intraperitoneal injection of scopolamine was also significantly recovered by ginsenosides Rg3(S) and Rg5/Rk1. Among the three ginsenosides tested in this study, Rg5/Rk1 enhanced the memory function of mice most effectively in both the ethanoland scopolamine-induced amnesia models. Moreover, the latency period of the Rg5/Rk1-treated mice was 1.2 times longer than that of the control (no amnesia) group in both models, implying that Rg5/Rk1 may also exert beneficial effects in the normal brain. We also evaluated the effects of these ginsenosides on the excitotoxic and oxidative stress-induced neuronal cell damage in primary cultured rat cortical cells. The excitotoxicity induced by glutamate or Nmethyl- D-aspartate (NMDA) was dramatically inhibited by the three ginsenosides. Rg3(S) and Rg5/Rk1 exhibited a more potent inhibition of excitotoxicity than did Rg3(R). In contrast, these ginsenosides were all ineffective against the H2O2- or xanthine/xanthine oxidase-induced oxidative neuronal damage. Taken together, these results indicate that ginsenosides Rg3(S) and Rg5/Rk1 significantly reversed the memory dysfunction induced by ethanol or scopolamine, and their neuroprotective actions against excitotoxicity may be attributed to their memory enhancing effects.

중국 호텔 인터넷 이용자의 재구매 의도에 미치는 인과관계

포형(Ying Bao),김영태(Young Tae Kim),최현정(Hyun Jung Choi) 한국관광연구학회 2012 관광연구저널 Vol.26 No.5

본 연구의 목적은 호텔 홈페이지를 이용한 경험이 있는 중국인 고객을 대상으로 인터넷 서비스품질, 인터넷 고객만족, 고객 신뢰, 그리고 재구매 의도의 구조적인 관계를 분석하고, 이에 따른 효과적인 대책을 논하는 것이다. 실증적인 분석을 위한 표본추출을 위하여 중국의 북부와 남부, 그리고 중심부에 각각 위치한 대표적인 도시와 호텔을 선정하여 약2주 동안 호텔 고객을 대상으로 설문조사를 실시하였고, 이에 따라 362개의 유효 표본이 본 분석에 활용되었다. 본 연구의 자료 분석을 위하여 SPSS(12.0)과 AMOS(5.0)이 활용하였으며, 빈도분석, 신뢰도 분석, 확인적 요인 분석 그리고 구조적 모형 분석이 진행되었다. 분석 결과, 인터넷 서비스품질의 5개 요인 중 4개 요인(정보품질, 고객개인화, 편리성, 반응성)이 인터넷 고객만족에 유의한 영향을 미치는 것으로 나타났다. 또한 인터넷 고객만족은 고객 신뢰와 재구매 의도에 유의한 영향을 미치는 것으로 나타났으며, 고객 신뢰는 재구매 의도에 긍정적인 영향을 미치는 것으로 확인되었다.

The Reflux Symptoms Before and After Peroral Endoscopic Myotomy of Chinese Patients With Achalasia

( Ying Xu ),( Yan Wang ),( Yun Bao ),( Ting Yu ),( Yurong Tang ),( Lin Lin ),( Liuqin Jiang ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2021 Journal of Neurogastroenterology and Motility (JNM Vol.27 No.3

Background/Aims Achalasia is a rare disease, but the incidence is increasing recently. Peroral esophageal myotomy (POEM) is an effective treatment. Regurgitation is a common symptom before and after POEM. Our aim is to investigate the factors related to preoperative and postoperative reflux symptoms. Methods Our study was retrospective. The achalasia patients diagnosed by high-resolution manometry and gastroscopy were divided into reflux group and non-reflux group before and after POEM, respectively. General information, symptoms, POEM information, and manometric results were compared. Results (1) Ninety-six of 130 patients had reflux symptoms before POEM. The lower esophageal sphincter pressure (LESP) in the reflux group was significantly higher than the non-reflux group (P = 0.023), while integrated relaxation pressure (IRP) was similar. The reflux group had longer esophagus than the non-reflux group (P = 0.006). Reflux symptoms were not related to subtypes of achalasia. (2) Twenty-five of 84 patients had reflux symptoms after POEM. Postoperative Eckardt scores, LESP, and 4-second IRP (4sIRP) were significantly lower than the preoperative values (P < 0.001). The preoperative values and POEM information were similar between the postoperative 2 groups and there was no significant difference in the presence of preoperative reflux symptoms between 2 groups. The postoperative LESP and 4sIRP were similar between the 2 groups, however, the postoperative UESP was significantly higher in the reflux group than the non-reflux group (P = 0.042). The non-reflux group had more declines in Eckardt scores and LESP than the reflux group. Conclusions The reflux symptoms of achalasia patients without treatment were mainly due to food retention. The postoperative reflux symptoms were not the sign of the excessive relaxation of lower esophageal sphincter. (J Neurogastroenterol Motil 2021;27:377-389)

Self-care Experiences of Adolescents with Spinal Muscular Atrophy

Bao-Huan Yang,Chia-Ying Chung,Wen-Chin Weng,Kao-Wen Lo,Yuh-Shiow Li 한국간호과학회 2021 Asian Nursing Research Vol.15 No.4

Purpose: We examined the self-care experiences of adolescents with spinal muscle atrophy (SMA) and their perceptions of the interactions between their body and the environment. Methods: We interviewed ten adolescents with SMA aged 13-18 years regarding personal care practices. Purposive sampling was conducted in two medical centers in northern Taiwan. Data were analyzed using the Giorgi analysis method. Results: Four constitutions were identified: (1) limited space for independent development, (2) multiple reconstructions of self-image to improve physical ability, (3) self-care of disease, and (4) developing activity styles to accommodate social culture. Conclusion: The self-care lived experiences of patients reflect dynamic changes in the body and environment. Self-existence was exhibited by adjustment, practice, and creativity of physical activity to integrate into society. Nursing staff should understand the self-care experiences and needs of adolescents with SMA to develop a database of self-care skills. This study recommended that nursing staff improve their ability to guide patients in taking care of themselves by developing body awareness self-care courses and individual care plans in response to various stages of disability to help patients delay deterioration, realize their physical potential, and promote independence and social development.