심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

서울지역 여대생들의 떡에 대한 인식 및 섭취 실태 조사

김영순,오순덕,김향미,신지홍,김현경,배유미,조규영,조성환 高麗大學校 倂設 保健大學 保健科學硏究所 2003 保健科學論集 Vol.29 No.1

This study conducted a survey of the improvement and popularization for Korea Rice Cakes, a Korean traditional food, on two hundred women students in five colleges in Seoul, Korea. It was investigated to get the information of the recognition, preference, and consumption patterns for the succession and improvement of Korean Rice Cakes. The results are as follows: 1. A portion of 41.5% among responders knows from three to six kinds of Korean Rice Cakes, and of 28% can make at least one among the kinds of Korean Rice Cakes. 2. A portion of 91% recognizes that Korean Rice Cakes are one of well-nourished food, but of 42% answers that Korean Rice Cakes cannot be replaced with the principle food. Also, there are no significant differences in the recognition of Korean Rice Cakes as the kinds of religions. 3. The frequency of purchase is about one or twice in a month (35.5%). Consumers (36.5%) intend to purchase Korean Rice Cakes for the commemoration of national holidays or anniversaries. 4. The reasons for the preference of other foods more than Korean Rice Cakes are the easiness of purchase (36.0%), the deliciousness (21.5%), the diverse kindness (17.5%), and the proper package (9.5%). It is important that constant and particular school / home education is need to improve and success Korean Rice Cakes.

항암화학요법 관련 오심과 구토 완화를 위한 비약물요법에 관한 연구동향

소향숙,최자윤,조인숙,김영재,김지영,김애숙,김옥미,김춘심,김현오,설영애,안정옥,이애리,이영자 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: Purposes of this study were to understand the current trends on complementary therapy in relieving chemotherapy-induced nausea and vomiting and to suggest the future research direction. Method: Subjects were selected on CINAHL, MEDLINE, Korean Academy Data Base from 1980 to 2001 which used nausea, vomiting, chemotherapy and complementary therapy as key words in experimental studies. Eight korean articles and twenty-one international articles were analyzed in terms of general characteristics, research methods, and types of complementary therapy. Data were analyzed by using descriptive statistical methods. Result: Since 2000, researchers have more actively used complementary therapy. In subject characteristics, mean age was 35.5 years old, 45% of the researchers were performed with high level of incidence of chemotherapy induced vomiting, 14% of them set limit of consecutive cycle during research, and 65% of them did not comment the selecting criteria of sample. About 60% of them were designed post-test only control group: 35% used INV by Rhodes, 31% used Likert scale, and 24% used VAS for dependent variable. Muscle relaxation therapy was mostly applied for relief of nausea and vomiting. Conclusion: Further studies will be needed to control extrinsic variables affecting nausea and vomiting in research design and to accumulate evidence with studies applying various complementary therapies.

포로 체험의 재구성과 기억의 공유

김미향 ( Kim Mi Hyang ) 아시아문화학술원 2017 인문사회 21 Vol.8 No.4

이 연구는 공적 기억인 역사 속에 숨어 있는 개인의 체험과 기억을, 문학을 통해 해석해 냄으로써 한국전쟁 포로 소설의 특징과 존재 방식을 재구해 내고자 했다. 이를 위해 Ⅱ장에서는 전쟁의 목적조차 잃은 채 과열된 갈등으로 폭력과 살육으로 폭주하는 포로들의 모습을 통해 이데올로기의 허구성을 증명하였고, Ⅲ장에서는 전쟁의 직접성에서 벗어나 일상이 전경화 된 상태에서 포로 체험의 기억을 타자와 공유하면서 공감을 불러일으키는 과정을 확인하였다. 포로들의 삶이 개인의 특별한 체험에 불과하다고 치부하거나 또는 `포로`라는 이름으로 이들의 삶을 일률적으로 규정짓지 않고 포로들 각각의 삶을 통해 그 함축된 의미를 고찰해낸다면 이데올로기 대결의 가장 중심에 놓인 핵심적 인물인 포로들이 전쟁으로 고통 받고 희생된 대표적 `비극적 삶의 주체`임을 증명하게 될 것이다. 그리고 이러한 과정을 통해 역사를 보는 시각은 더 다양해지고 우리 전쟁문학의 스펙트럼은 더욱 넓어질 것이다. In the situation tied to the immediacy of war, reorganization of repetitive captive experiences led to tragic ending by making captives` scars fixed. Also, fierce conflicts among captives in the prison camp resulted in cruel slaughter and violence, and Southern Government which had left them in the danger of murder and violence used them politically under the pretense of the release, never considering captives. Captives forgot the purpose of the war and rushed to the violence and slaughter without telling friends from enemies due to excessive conflicts among them, which obviously reveals unrealistic ideology. On the other hand, starting to share the memory of the captive experiences in a state that daily lives are in the foreground, the characters in Kim`s work who are free themselves from the immediacy of war arouse others` sympathy. However, whether the ending is tragic or reconciliatory, it has in common the fact that they are not courageous soldiers or vicious enemies who engaged in the battle field but are normal people and captives of Korean War. They show us that the violence in the prison camp wasn`t individual choice but inevitable result of the war, along with the tragic ending they chose. This makes us check that the captives, key characters placed in the center of ideology confrontation, may be actually representative victims by the ideology, all of whom were most irrelevant to the ideology.

자궁경부 상피내 병변 환자에서 LEETZ 원추절제술 후 HPV DNA Test 추적 검사의 임상적 의의

김미향,이태화,오영림,이천준,김원규 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Objective: This study was performed to investigate the clinical significance and timing of HPV test for follow up of patients after LEETZ treatment in CIN lesion Methods: From January 2005 to December 2008, 105 patients with CIN were treated with LLETZ at Kosin university gospel hospital, Busan, Korea. After LEETZ, HPV test and Pap smear were done after 3months. Every HPV typing was done by HPV DNA chip. Results: A total of 105 cases were enrolled in this study. There were 25 patients with CINⅠ, 38 with CINⅡ, 42 with CINⅢ. Mean age was 41.95 years (range, 23-71 years) at the time of LEETZ. Positive HPV DNA was found about 72.4% before LEETZ procedure and subtype 16 was the most common type (10.5%), follow by subtype 18 (6.6%) and subtype 58 (6.6%). During follow up after LEETZ, 65.6% had negative result after 3 month, 72.1% after 6 month, 73.9% after 9 month, 77.1% after 12 month, 79.2% after 15 month, 79.6% after 18 month, and 80.4% after 24 month. There were 2 cases of abnormal pap smear among persistent positive HPV DNA cases and no abnormal pap smear results in negative HPV DNA cases during follow up. Conclusion: Positive HPV DNA was found about 72.4% before LEETZ procedure and subtype 16 was the most common type (10.5%), follow by subtype 18 (6.6%) and subtype 58 (6.6%). We suggest that HPV DNA test is recommended per 6 Months, and close observation is recommended in persistent positive HPV DNA test cases.

임신에 대한 간호계와 비간호계 여대생의 의식조사 연구

김미향 순천청암대학 1988 論文集-順川看護專門大學 Vol.12 No.-

During 1st to 15th June, 1987, I have collected data from 158 persons university's girl in Kwang Joo in order to grasp their knowledge about pregnancy. From the data, I have gained the following results: The distribution of the respondent' general education 98 persons and the professional nursing 60 persons. 1. General Characteristics 65.2 percent, the higest of the respondents wanted to marry by means of a love marriage being arrarged by a go-between. 26-30 day type menstruation cycle was 53.8 percent and menarche's mean was 15.2 years old. 2. Knowledge about pregnancy The results that show significant difference are summarized as follows: 83.7 percent of general education and 98.3% of proffssional nursing answered to the question of sex determination factor by male sex chromosome. This showed a significant difference between both group.(x^2=8.43, df=2, p<.05) There was just recognized is general education 48.0 percent and proffssional nursing 81.7% in the expected date of confinement accounts. (E.D.C : Last menstruation month-3 and menstruation first of beginning day+7) This showed a significant difference between both group. (x^2=20.08, df=3, p<.001) High pregnancy frequency give bad effect on mother's health. We found a significant difference between both group. (x^2=9.03. df=3, p<.05) To the question of how they came to know the methods of pregnancy knowledge, general education(62.2%) and professional nursing(83.3%) answered "from a technical books". This showed a considerable difference between both group. (x^2=14.17, df=5, p<.05)

한국인 여성의 자궁경부암에서 PCR을 이용한 Human papillomavirus 아형의 검출에 관한 연구

김향우,전호종,김윤신,장원재,이미숙,이미자 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The polymerase chain reaction (PCR) was used for detection of human papillomavirus (HPV) in paraffin-embedded tissue. Tissues stored as paraffin blocks were a potential source of DNA for retrospective clinicogenetic analysis. Sections in 6 ㎛ (three or five) thick cut from paraffin blocks of 29 uterine cervix dysplasia, 34 uterine cervix carcinoma in situ, 42 uterine cervix squamous cell carcinoma and 10 uterine cervix metaplasia samples were deparaffinized and incubated at 37℃ for 48hrs. in the lysis solution. Following organic extraction and alcohol precipitation, and extracted DNAs were identified by gel electrophoresis. These DNAs carried out PCR. After repeated cycles of specific oligonucleotideprimed extension of viral DNA with thermophilic DNA polymerase. the type of HPV present was then determined on the basis of the size of the ethidium-bromide stained band visible after agarose gel electrophoresis : for HPV 6 is 263 bp. for HPV 11 is 144 bp, for HPV 16 is 601 bp. and for HPV 18 is 360 bp. This study detected HPV DNA in four cases. which broke down to HPV 16 in two case of uterine cervix carcinoma in situ and two case of uterine cervix squamous cell carcinoma, no HPV type in the remainder. Histologically, HPV types 6, 11 are mainly detected in benign genital warts and lowgrade cervical dysplasias, squamous cell carcinoma frequently contained HPV 16, whereas, HPV 18 was present in adenocarcinoma, adenosquamous cell carcinoma and small cell carcinoma of the cervix. These results indicate that cervical cancers might be clinically influenced by the differences in sutible of the infecting HPV.

조리 방법에 따른 호박 중의 클로로필과 카로틴 함량 변화

김동석,小机信行,김미향 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.6

This study was performed to investigate changes of chlorophyll and carotene contents by cooking methods and parts of pumpkin(zucchini, green pumpkin, and sweet pumpkin). The highest chlorophyll content of 3-parts(peel, cortex, and seed), all of pumpkin was peel part for chlorophyll contents by cooking methods of all kinds of pumpkin. It was the highest when cooked by non-batter frying. Carotene was relativity stable with cooking. But in case of sweet pumpkin, steaming cooking method was reduced carotene contents. β-carotene of zucchini, green pumpkin, and sweet pumpkin was higher than standard contents.

쑥이 흰쥐의 Collagen 합성에 미치는 효과

김미향 신라대학교 자연과학연구소 2004 自然科學論文集 Vol.13 No.-

폐경을 앞둔 여성에게는 폐경을 전후하여 지속되는 골 손실 가속화 등의 문제점을 가지고 있다. 그러므로 본 연구에서는 인위적 폐경을 유발시킬 수 있는 난소 절제 쥐에서의 골손실의 유무를 결합조직 중의 collagen 함량 변화를 통하여 그 효과를 검토하였다. 생화학적 골 대사 지표인 혈 중 ALP 활성은 난소 절제로(OVX-control)로 인하여 증가하는 반면, 난소절제 후 쑥 추출물 투여(OVX-AP)로 혈 중 ALP 활성은 감소하는 경향을 보여 골 흡수를 저하시킬 수 있는 가능성을 나타내었다. 한편 난소 절제 후 쑥 추출물 투여에 의해 피부 조직과 골·연골 조직에서 collagen 함량이 증가하는 경향을 보였고, 쑥 추출물 투여군 모두에서 collagen 합성량이 유의적으로 증가하였다. 쑥 추출물이 에스트로겐 감소로 인한 collagen 합성저하를 회복시킨 본 실험 결과로 미루어보아 쑥 중의 phytoestrogen이 에스트로겐 유사효과를 나타냄을 알 수 있었고, 외인성 에스트로겐 투여로 인한 부작용을 줄여 줄 수 있을 것으로 기대된다. 이상과 같이 폐경기 여성에게 쑥 추출물의 섭취는 폐경으로 인한 장애를 감소시키기 위하여 에스트로겐의 장기간 복용에 의한 부작용을 쑥 중의 phytoestrogen이estrogen 대체 작용을 함으로써 그 부작용을 감소시켜 줄 것으로 사료되며, 이들의 구조 및 기전에 대해서는 앞으로 연구가 더 필요한 것으로 생각된다. The aim of this study was to evaluate the effects of Artemisia Princeps Var. Orientalis (AP) on the collagen content of the connective tissues in ovariectomized rats. From day 2 until day 37 after the ovariectomy, Sprague-Dawley female rats were randomly assigned to the following groups : sham-operated rats (Sham), ovariectomized control rats (OVX-control), and ovariectomized rats supplemented with the AP 50 mg/kg bw/day (OVX-AP50). The AP ethanol extracts were orally administrated 1 mL per day. The OVX rats were significantly heavier than the sham-operated rats at all time points, but supplementation with the AP extracts tended to gain weight less than OVX-control. Supplementation with the AP extracts prevented a decrease in the collagen level in bone and cartilage tissues. These results are consistent with the conclusions based on the estrogenic activities of AP. Therefore, it may be used to possibly improve the quality of life in menopausal women.

샐러드 드레싱에 대한 소비자의 기호도와 이용 실태 조사 연구 : 대구지역을 중심으로

김미향,이수진,김향희 동아시아식생활학회 2001 동아시아식생활학회지 Vol.11 No.5

This study was conducted to evaluate the consumers' preferences and perceptions on the salad and its dressings, via a total of 401 subjects in the Taegu area on the basis of 5-point Likert scale. Anthropometric data included the total subjects consisted of 66 females and 335 males: categorized by age, 20's (103), 30's (135). 40's (83), over 50's (80). The average size of family was 4.2 persons. According to BMI, 64.8% of the subjects belonged to the normal group. The age group that liked salad most was the 20's group, which was also the group that ate salad most frequently. The salad was perceived as food item which is convenient to eat(3.83), healthy(3.63), easy to prepare(3.63) and delicious(3.61) but rarely as a expensive item(2.44). The ingredients, used for salad preparation included fruits and vegetables (3.19) such as tomato and cucumber, Among the salad dressings, the fruit dressing was preferred the most(4.59), while the mayonnaise the least(3.59). The mayonnaise was perceived as an item with highest calories(4.1). and hardly perceived as a luxury item(2.78). The preference for the fruits dressing was highly correlated with the degree of education (p<0.05), age(p<0.001) and BMI(p<0.001). The higher the degree of education, the lower the level of age group, and the lower the BMI, the higher preference for the fruit salad dressing was demonstrated.