Phenolic components from the stem of Acanthopanax koreanum and their inhibitory effects on NF-kappa B.

Sun, Ya Nan,Li, Wei,Yan, Xi Tao,Yang, Seo Young,Song, Seok Bean,Kim, Young Ho Japan Society for Bioscience, Biotechnology, and A 2014 Bioscience, Biotechnology, and Biochemistry Vol.78 No.3

<P>Two novel phenolic glucosides (1-2), as well as seven known compounds (3-9), were isolated from the stem of Acanthopanax koreanum; their chemical structures were determined by chemical and spectroscopic methods and subsequently compared with previously reported data. Their inhibition of nuclear factor kappa B (NF-κB) was measured in human embryonic kidney (293T) cells by using an NF-κB luciferase assay.</P>

Chemical constituents from the root of Polygonum multiflorum and their soluble epoxide hydrolase inhibitory activity

Ya Nan Sun,Wei Li,김장훈,Xitao Yan,김지은,양서영,김영호 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

Fourteen compounds were isolated from amethanol extract of Polygonum multiflorum roots, and theirstructures were elucidated by comparing spectroscopic datato published spectra. The inhibitory effects of the isolatedcompounds on soluble epoxide hydrolase (sEH) were thenevaluated. Compounds 1–7 inhibited sEH activity potently,with IC50 values ranging from 6.2 ± 0.5 to 48.6 ±3.1 lM. Moreover, a kinetic analysis of compounds 1–7revealed that the inhibitory actions of compounds 1, 3 and4 were non-competitive, whereas those of compounds 2and 5–7 were mixed-type.

Trypanosoma brucei Inhibitory Activities of Vietnamese Medicinal Plants

Ya Nan Sun,Seo Young Yang,Hong Xu Li,Xiang Dong Su,Gyongseon Yang,Joo Hwan No,Jong Seong Kang,Young Ho Kim 충남대학교 약학대학 의약품개발연구소 2016 藥學論文集 Vol.31 No.-

A large number of extracts from the medicinal plants growing in Vietnam were screened for their in vitro antitrypanosomal activity toward Trypanosoma brucei. Among them, eighty-seven extracts showed the significant antitrypanosomal activity. Especially, the extracts from Neocinnamomum delavayi, Mallotus cuneatus, and Mallotus api exhibited the highest inhibitory activity against T. brucei (IC50: 0.16, 0.49 and <0.05 μg/mL). Cephalotaxus mannii, Pittosporum floribundum, Cleistanthus aff. annamensis, Wendlangia tinctoria subsp. barbatus, Maesa parvifolia, Alpinia malaccensis, Glycosmis scandens, Mallotus yunnanensis, and Maesa sp also presented potent T. brucei inhibitory activity, with IC50 value ranging from 1.14 to 4.98 μg/mL. Based on these results of screening study, the extracts from N. delavayi, M. cuneatus, M. api, C. mannii, P. floribundum, C. aff. annamensis, W. tinctoria subsp. barbatus, M. parvifolia, A. malaccensis, G. scandens, M. yunnanensis, and Mallotus sp. might be the rich source of antitrypanosomal components. Therefore, further phytochemistry and pharmacological study of these medicinal plants should be researched and developed.

NF-κB Inhibitory Activities of Phenolic and Lignan Components from the Stems of Acanthopanax divaricatus var. albeofructus

Ya Nan Sun,Wei Li,Seo Young Yang,Young Ho Kim 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Nuclear Factor Kappa B Activation and Peroxisome Proliferator-activated Receptor Transactivational Effects of Chemical Components of the Roots of <i>Polygonum multiflorum</i>

Sun, Ya Nan,Li, Wei,Song, Seok Bean,Yan, Xi Tao,Yang, Seo Young,Kim, Young Ho Medknow PublicationsMedia Pvt Ltd 2016 Pharmacognosy magazine Vol.12 No.45

<P><B>Background:</B></P><P><I>Polygonum multiflorum</I> is well-known as “Heshouwu” in traditional Chinese herbal medicine. In Northeast Asia, it is often used as a tonic to prevent premature aging of the kidney and liver, tendons, and bones and strengthening of the lower back and knees.</P><P><B>Objective:</B></P><P>To research the anti-inflammatory activities of components from <I>P. multiflorum</I>.</P><P><B>Materials and Methods:</B></P><P>The compounds were isolated by a combination of silica gel and YMC R-18 column chromatography, and their structures were identified by analysis of spectroscopic data (1D, 2D-nuclear magnetic resonance, and mass spectrometry). The anti-inflammatory activities of the isolated compounds 1−15 were evaluated by luciferase reporter gene assays.</P><P><B>Results:</B></P><P>Fifteen compounds (1–15) were isolated from the roots of <I>P. multiflorum</I>. Compounds 1−5 and 14−15 significantly inhibited tumor necrosis factor-α-induced nuclear factor kappa B-luciferase activity, with IC<SUB>50</SUB> values of 24.16-37.56 μM. Compounds 1−5 also greatly enhanced peroxisome proliferator-activated receptors transcriptional activity with EC<SUB>50</SUB> values of 18.26−31.45 μM.</P><P><B>Conclusion:</B></P><P>The anthraquinone derivatives were the active components from the roots of <I>P. multiflorum</I> as an inhibitor on inflammation-related factors in human hepatoma cells. Therefore, we suggest that the roots of <I>P. multiflorum</I> can be used to treat natural inflammatory diseases.</P><P><B>SUMMARY</B></P><P><P>This study presented that fifteen compounds (1-15) isolated from the roots of Polygonum multiflrum exert signifiant anti inflmmatory effects by inhibiting TNF α induced NF κB activation and PPARs transcription.</P></P> >[FIG OMISSION]</BR><P>Abbreviation used: NF κB: Nuclear factor kappa B, PPARs: Peroxisome proliferator activated receptors, PPREs: Peroxisome proliferator response elements, TNF α: Tumor necrosis factor α, ESI-MS: Electrospray ionization mass spectrometry, HepG2: Human hepatoma cells</P>

Antioxidant and anti-osteoporotic effects of anthraquinones and related constituents from the aqueous dissolved <i>Aloe</i> exudates

Sun, Ya Nan,Li, Wei,Lee, Sang Hyun,Jang, Hae Dong,Ma, Jin Yeul,Kim, Young Ho Taylor & Francis Health Sciences 2017 NATURAL PRODUCT RESEARCH Vol.31 No.23

<P>.</P>

Chemical Components from <i>Aloe</i> and their Inhibition of Indoleamine 2, 3-dioxygenase

Sun, Ya Nan,Li, Lin Ying,Li, Wei,Kang, Jong Seong,Hwang, Inkyu,Kim, Young Ho Medknow PublicationsMedia Pvt Ltd 2017 Pharmacognosy magazine Vol.13 No.49

<P><B>Background:</B></P><P>In Korea, <I>Aloe</I> is routinely ingested as a traditional medicine or as a component of health beverages.</P><P><B>Objective:</B></P><P>To research the inhibition of indoleamine 2, 3-dioxygenase (IDO) activities of components from <I>Aloe</I>.</P><P><B>Materials and Methods:</B></P><P>the compounds were isolated by a combination of silica gel and YMC Rp-18 column chromatography, and their structures were identified by analysis of spectroscopic data (1D, 2D-NMR, and MS). All of the isolated compounds were examined for their ability to inhibit IDO, which actively suppresses immune functions by catalyzing the rate limiting reaction in the conversion of tryptophan to kynurenine.</P><P><B>Results:</B></P><P>In this phytochemical study, 18 known compounds were isolated from aqueous dissolved <I>Aloe</I> exudates. All of the isolated compounds were examined for their ability to inhibit IDO activities for a series of anthraquinone derivatives (1-7) isolated from the <I>Aloe</I> extract; the IC<SUB>50</SUB> values of these compounds ranged from 39.41 to 53.93 µM. Enzyme kinetic studies of their modes of inhibition indicated that all of the compounds were uncompetitive inhibitors.</P><P><B>Conclusion:</B></P><P>The aqueous dissolved <I>Aloe</I> exudate can be used as a source of novel natural IDO inhibitors and merit testing as therapeutic agents in the treatments of cancer and immunopathologic diseases, such as autoimmune, inflammatory, and allergic disorders.</P><P><B>SUMMARY</B></P><P><P>In this study, 18 known compounds were isolated from aqueous dissolved Aloe exudates. All of the isolated compounds were examined for their ability to inhibit indoleamine 2, 3-dioxygenase (IDO) activities for a series of anthraquinone derivatives (1−7) isolated from the Aloe extract.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviation used:</B> IDO: inhibit indoleamine 2, 3-dioxygenase, TMS: tetramethylsilane, HMQC: heteronuclear multiple quantum correlation, HMBC: heteronuclear multiple bond correlation, COSY: 1H-1H correlation spectroscopy, ESI-MS: Electrospray ionization mass spectrometry, DMSO: dimethyl sulfoxide</P>

아파트 주변 공원녹지의 속성 가치

손아남(Sun Ya nan),정경수(Kyeong Soo Jeong) 건국대학교 경제경영연구소 2014 商經硏究 Vol.39 No.1

이 연구에서는 비시장재화의 가치추정법인 헤도닉가격기법을 이용하여 중국 정저우시 아파트 시장을 대상으로 아파트 가격에서 차지하는 주택 특성 변수들의 잠재가격을 추정하였다. 특히 최근 들어 중국 주민들의 환경가치 선호경향이 높아짐에 따라 아파트 구입 시 단지 주변에 공원녹지가 있는 아파트를 선호하는 추세를 감안하여 아파트 주변 공원녹지의 경제적 가치를 추정하였다. 설명력이 가장 높은 것으로 나타난 준로그 함수형태의 헤도닉가격 방정식을 추정한 결과, 준공연도가 1% 최근 년도일수록 아파트 가격은 11.15% 상승하고, 아파트 크기가 1% 증가할 때 아파트 가격은 0.87% 상승하였다. 공원면적은 통계적으로 유의적인 변수로 증명되었으며, 지역 평균값보다 1% 증가할 경우 총편익은 2847.50위안(50만원)으로 계산되었다. 공원면적이 최대 20%까지 증가할 경우를 가정할 때의 총편익이 55,670.31위안(969만원)으로 계산되었다. 이는 표본아파트의 평균가격인 82.69만 위안(1억4 천만 원)의 6.73%에 해당된다. 이 연구는 정저우와 같은 중국 중소도시에서 공원면적과 같은 환경요소가 아파트가격을 결정하는데 중요한 요인임을 밝혔다. This study investigates the implicit marginal price of the environmental characteristics which affect the market price of apartments in Zhengzhou. The hedonic price method is applied to estimate the implicit value of the adjacent green spaces which is assumed to be beneficial to promote the health of apartmental residents. To estimate the hedonic function, four functional forms are applied and the semi-log model was selected as the best one. The estimation result shows that the construction year and the apartment space were highly significant factor to affect the price of Zhengzhou s apartments. The area of the adjacent green spaces was proved to be the statistically significant influential variable. As the area increases 20% from average, the welfare increases about 9,690 thousand Won, or 6.7% of the average apartment price in Zhengzhou. This result implies that the environmental factor such as adjacent green spaces is an important determinant of housing price in medium size cities in China.

Molecular identification and sex distribution of two chemosensory receptor families in Athetis lepigone by antennal transcriptome analysis

Ya-Nan Zhang,Ji-FangMa,Liang Sun,Zhi-PingDong,Zhao-Qun Li,Xiu-Yun Zhu,YiWang,LuWang,Dao-Gui Deng,Jin-Bu Li 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3

Chemosensory receptors play central roles in insect behavior and can potentially be used as new targets for pest control. Athetis lepigoneMöschler (Lepidoptera: Noctuidae) is a polyphagous insect pest found in many countries in Europe and Asia. However, systemic identification of the chemosensory receptors in the antennae has not been reported. In the present study, we obtained the antennal transcriptome of A. lepigone using Illumina sequencing technology and identified 80 candidate chemosensory receptors, including 61 transcripts encoding for odorant receptors (ORs) and 19 for ionotropic receptors (IRs). This result is similar to that reported for other lepidopteran species, suggesting that our approach successfully identified ORs and IRs of A. lepigone. RT-PCR was used to determine the sex distribution patterns of all OR and IR genes. Some OR and IR genes showed male- or femalespecific/ predominant expression. Our results help understand the functions of chemosensory receptor genes in A. lepigone as well as other insects, and suggest that these genes could be potential targets for developing environment-friendly behavioral antagonists and pesticides against A. lepigone.

De novo assembly and characterization of antennal transcriptome reveal chemosensory system in Nysius ericae

Ya-Nan Zhang,Xiu-Yun Zhu,Qian Zhang,Cai-Yun Yin,Zhi-PingDong,Ling-Hua Zuo,Dao-Gui Deng,Liang Sun,Xiao-Ming Li 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.4

Chemosensory system plays important roles in insect behaviors, some key genes governing chemosensation can potentially be used as novel targets for pest control. Nysius ericae (Hemiptera: Lygaeidae) is a polyphagous insect pest found throughout Eurasia countries which causes damage to many important crops. Little is known about the chemosensory mechanisms of host location and mate choice in N. ericae at themolecular level. Here, we assembled the antennal transcriptome of N. ericae using Illumina sequencing technology. Analysis of the transcriptome data identified 148 candidate chemosensory genes, including 28 for odorant binding proteins (OBPs), 16 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 83 odorant receptors (ORs), 12 ionotropic receptors (IRs), and 7 gustatory receptors (GRs). Our results greatly improve the gene inventory for N. ericae and provide valuable resources for analyzing the functions of some key genes. This information will aid in the development of better understanding of chemosensation in N. ericae, and indicate novel targets for managing its impact on agriculture. Chemosensory system plays important roles in insect behaviors, some key genes governing chemosensation can potentially be used as novel targets for pest control. Nysius ericae (Hemiptera: Lygaeidae) is a polyphagous insect pest found throughout Eurasia countries which causes damage to many important crops. Little is known about the chemosensory mechanisms of host location and mate choice in N. ericae at themolecular level. Here, we assembled the antennal transcriptome of N. ericae using Illumina sequencing technology. Analysis of the transcriptome data identified 148 candidate chemosensory genes, including 28 for odorant binding proteins (OBPs), 16 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 83 odorant receptors (ORs), 12 ionotropic receptors (IRs), and 7 gustatory receptors (GRs). Our results greatly improve the gene inventory for N. ericae and provide valuable resources for analyzing the functions of some key genes. This information will aid in the development of better understanding of chemosensation in N. ericae, and indicate novel targets for managing its impact on agriculture.Chemosensory system plays important roles in insect behaviors, some key genes governing chemosensation can potentially be used as novel targets for pest control. Nysius ericae (Hemiptera: Lygaeidae) is a polyphagous insect pest found throughout Eurasia countries which causes damage to many important crops. Little is known about the chemosensory mechanisms of host location and mate choice in N. ericae at themolecular level. Here, we assembled the antennal transcriptome of N. ericae using Illumina sequencing technology. Analysis of the transcriptome data identified 148 candidate chemosensory genes, including 28 for odorant binding proteins (OBPs), 16 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 83 odorant receptors (ORs), 12 ionotropic receptors (IRs), and 7 gustatory receptors (GRs). Our results greatly improve the gene inventory for N. ericae and provide valuable resources for analyzing the functions of some key genes. This information will aid in the development of better understanding of chemosensation in N. ericae, and indicate novel targets for managing its impact on agriculture.