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Metagenomic Insight into Lignocellulose Degradation of the Thermophilic Microbial Consortium TMC7
( Yi Wang ),( Chen Wang ),( Yonglun Chen ),( Beibei Chen ),( Peng Guo ),( Zongjun Cui ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8
Biodegradation is the key process involved in natural lignocellulose biotransformation and utilization. Microbial consortia represent promising candidates for applications in lignocellulose conversion strategies for biofuel production; however, cooperation among the enzymes and the labor division of microbes in the microbial consortia remains unclear. In this study, metagenomic analysis was performed to reveal the community structure and extremozyme systems of a lignocellulolytic microbial consortium, TMC7. The taxonomic affiliation of TMC7 metagenome included members of the genera Ruminiclostridium (42.85%), Thermoanaerobacterium (18.41%), Geobacillus (10.44%), unclassified_f__Bacillaceae (7.48%), Aeribacillus (2.65%), Symbiobacterium (2.47%), Desulfotomaculum (2.33%), Caldibacillus (1.56%), Clostridium (1.26%), and others (10.55%). The carbohydrate-active enzyme annotation revealed that TMC7 encoded a broad array of enzymes responsible for cellulose and hemicellulose degradation. Ten glycoside hydrolases (GHs) endoglucanase, 4 GHs exoglucanase, and 6 GHs β-glucosidase were identified for cellulose degradation; 6 GHs endo-β-1,4-xylanase, 9 GHs β-xylosidase, and 3 GHs β-mannanase were identified for degradation of the hemicellulose main chain; 6 GHs arabinofuranosidase, 2 GHs α-mannosidase, 11 GHs galactosidase, 3 GHs α-rhamnosidase, and 4 GHs α-fucosidase were identified as xylan debranching enzymes. Furthermore, by introducing a factor named as the contribution coefficient, we found that Ruminiclostridium and Thermoanaerobacterium may be the dominant contributors, whereas Symbiobacterium and Desulfotomaculum may serve as “sugar cheaters” in lignocellulose degradation by TMC7. Our findings provide mechanistic profiles of an array of enzymes that degrade complex lignocellulosic biomass in the microbial consortium TMC7 and provide a promising approach for studying the potential contribution of microbes in microbial consortia.
Eigenvalues of Second-Order Vector Equations on Time Scales with Boundary Value Conditions
Yi Wang 한국전산응용수학회 2011 Journal of applied mathematics & informatics Vol.29 No.1
This paper is concerned with eigenvalues of second-order vector equations on time scales with boundary value conditions. Properties of eigenvalues and matrix-valued solutions are studied. Relationships between eigenvalues of different boundary value problems are discussed.
Ordinal Optimization Theory Based Planning for Clustered Wind Farms Considering the Capacity Credit
Yi Wang,Ning Zhang,Chongqing Kang,Qianyao Xu,Hui Li,Jinyu Xiao,Zhidong Wang,Rui Shi,Shuai Wang 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.5
Wind power planning aims to locate and size wind farms optimally. Traditionally, wind power planners tend to choose the wind farms with the richest wind resources to maximize the energy benefit. However, the capacity benefit of wind power should also be considered in large-scale clustered wind farm planning because the correlation among the wind farms exerts an obvious influence on the capacity benefit brought about by the combined wind power. This paper proposes a planning model considering both the energy and the capacity benefit of the wind farms. The capacity benefit is evaluated by the wind power capacity credit. The Ordinal Optimization (OO) Theory, capable of handling problems with non-analytical forms, is applied to address the model. To verify the feasibility and advantages of the model, the proposed model is compared with a widely used genetic algorithm (GA) via a modified IEEE RTS-79 system and the real world case of Ningxia, China. The results show that the diversity of the wind farm enhances the capacity credit of wind power.
Yi Wang,Hanhong Jiang,Pengxiang Xing 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.1
To enable a master–slave control independent microgrid system (MSCIMGS) to supply electricity continuously, the microgrid inverter should perform mode transfer between grid-connected and islanding operations. Transient oscillations should be reduced during transfer to effectively conduct a seamless mode transfer. This study uses a typical MSCIMGS as an example and improves the mode transfer strategy in three aspects: (1) adopts a status-tracking algorithm to improve the switching strategy of the outer loop, (2) uses the voltage magnitude and phase pre-synchronization algorithm to reduce transient shock at the time of grid connection, and (3) applies the hybrid-sensitivity H∞ robust controller instead of the current inner loop to improve the robustness of the controller. Simulations and experiments show that the proposed strategy is more practical than the traditional proportional–derivative control mode transfer and effective in reducing voltage and current oscillations during the transfer period.
Three New Non-reducing Polyketide Synthase Genes from the Lichen-Forming Fungus Usnea longissima
( Yi Wang ),( Juan Wang ),( Yong Hwa Cheong ),( Jae Seoun Hur ) 한국균학회 2014 Mycobiology Vol.42 No.1
Usnea longissima has a long history of use as a traditional medicine. Several bioactive compounds, primarily belongingto the polyketide family, have been isolated from U. longissima. However, the genes for the biosynthesis of these compounds areyet to be identified. In the present study, three different types of non-reducing polyketide synthases(UlPKS2, UlPKS4, andUlPKS6) were identified from a cultured lichen-forming fungus of U. longissima. Phylogenetic analysis of product templatedomains showed that UlPKS2 and UlPKS4 belong to group IV, which includes the non-reducing polyketide synthases with anmethyltransferase(MeT) domain that are involved in methylorcinol-based compound synthesis; UlPKS6 was found to belong togroup I, which includes the non-reducing polyketide synthases that synthesize single aromatic ring polyketides, such as orsellinicacid. Reverse transcriptase-PCR analysis demonstrated that UlPKS2 and UlPKS4 were upregulated by sucrose; UlPKS6 wasdownregulated by asparagine, glycine, and alanine.
The basic study of methanol to gasoline in a pilot-scale fluidized bed reactor
Yi Wang,Fengzhen Yuan 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.3
A fluidized bed reactor, used for methanol to gasoline (MTG), was designed followed the theory of gas–solid two-phase flow, and the effects of some factors, such as temperature, space velocity and theregeneration process, on the performance of MTG catalyst were systematically examined. The resultsshow that: heat and mass transfer can be effectively conducted in the fluidized bed reactor; with thereaction temperature was increased, the methanol conversion rate maintained at 100% and the yield ofgasoline gradually increased, then reached its highest value of 25.22% at 410 ℃, after that it began todecline; and the C5 aromatics content increased with temperature and reached its maximum value of49.86% at 430 ℃. With the weight space velocity was increased, the yield of gasoline firstly increased andthen decreased, while the C5 aromatics content was decreased; In addition, the effect of inner-regenerated process for used catalyst is very good. Low temperature can help to generate lighter olefinpolymer, the higher extent of hydrogen transfer and cracking of large molecules at middle temperature,the carbon deposition reaction and aromatization reaction of low carbon olefin occurred at highertemperature, all of these contributed the above mentioned rules. While the weight space velocity acts onthe performance of catalyst mainly via influencing the contact time and the carbon deposition reaction.
Yi Wang,Kai Wang,Jiajun Hu,Kang Si,Hongyan Xia 대한금속·재료학회 2023 ELECTRONIC MATERIALS LETTERS Vol.19 No.6
Thermal interface materials with high thermal conductivity are the best choice to solve the current overheating problem of electronic devices, and the choice of thermal conductive fi ller is a key factor aff ecting the performance of thermal interface composites. Hexagonal boron nitride sheet is considered to be ideal thermal conductive fi ller, but the thermal conductive eff ect of single fi ller is still insuffi cient to meet actual needs. Here, a new type of hybrid fi ller mixed with boron nitride sheet and boron phosphide particles is used to prepare epoxy based composites. When the fi ller volume fraction is 40%, the thermal conductivity of composites reaches 3.18 W m −1 K −1 . Under the same content, the thermal conductivity of the composites with mixed fi llers outperforms those with boron nitride sheet alone. At the same time, the composite material shows high breakdown strength of 46 kV mm −1 . This work provides a new way to prepare thermal interface materials with high thermal conductivity for modern electronic packaging.