RNA m5C modification upregulates E2F1 expression in a manner dependent on YBX1 phase separation and promotes tumor progression in ovarian cancer

Liu Xiaoyi,Wei Qinglv,Yang Chenyue,Zhao Hongyan,Xu Jie,Mobet Youchaou,Luo Qingya,Yang Dan,Zuo Xinzhao,Chen Ningxuan,Yang Yu,Li Li,Wang Wei,Yu Jianhua,Xu Jing,Liu Tao,Yi Ping 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

5-Methylcytosine (m5C) is a common RNA modification that modulates gene expression at the posttranscriptional level, but the crosstalk between m5C RNA modification and biomolecule condensation, as well as transcription factor-mediated transcriptional regulation, in ovarian cancer, is poorly understood. In this study, we revealed that the RNA methyltransferase NSUN2 facilitates mRNA m5C modification and forms a positive feedback regulatory loop with the transcription factor E2F1 in ovarian cancer. Specifically, NSUN2 promotes m5C modification of E2F1 mRNA and increases its stability, and E2F1 binds to the NSUN2 promoter, subsequently reciprocally activating NSUN2 transcription. The RNA binding protein YBX1 functions as the m5C reader and is involved in NSUN2-mediated E2F1 regulation. m5C modification promotes YBX1 phase separation, which upregulates E2F1 expression. In ovarian cancer, NSUN2 and YBX1 are amplified and upregulated, and higher expression of NSUN2 and YBX1 predicts a worse prognosis for ovarian cancer patients. Moreover, E2F1 transcriptionally regulates the expression of the oncogenes MYBL2 and RAD54L, driving ovarian cancer progression. Thus, our study delineates a NSUN2-E2F1-NSUN2 loop regulated by m5C modification in a manner dependent on YBX1 phase separation, and this previously unidentified pathway could be a promising target for ovarian cancer treatment.

A FEEDBACK-DRIVEN BUBBLE G24.136+00.436: A POSSIBLE SITE OF TRIGGERED STAR FORMATION

Liu, Hong-Li,Wu, Yuefang,Li, JinZeng,Yuan, Jing-Hua,Liu, Tie,Dong, Xiaoyi IOP Publishing 2015 The Astrophysical journal Vol.798 No.1

<P>We present a multi-wavelength study of the IR bubble G24.136+00.436. The J = 1-0 observations of (CO)-C-12, (CO)-C-13, and (CO)-O-18 were carried out with the Purple Mountain Observatory 13.7 m telescope. Molecular gas with a velocity of 94.8 km s(-1) is found prominently in the southeast of the bubble, shaped as a shell with a total mass of similar to 2 x 10(4) M-circle dot. It was likely assembled during the expansion of the bubble. The expanding shell consists of six dense cores, whose dense (a few of 10(3) cm(-3)) and massive (a few of 10(3) M-circle dot) characteristics coupled with the broad linewidths (>2.5 km s(-1)) suggest that they are promising sites for forming high-mass stars or clusters. This could be further consolidated by the detection of compact H II regions in Cores A and E. We tentatively identified and classified 63 candidate young stellar objects (YSOs) based on the Spitzer and UKIDSS data. They are found to be dominantly distributed in regions with strong molecular gas emission, indicative of active star formation, especially in the shell. The H II region inside the bubble is mainly ionized by a similar to O8V star(s), of the dynamical age of similar to 1.6 Myr. The enhanced number of candidate YSOs and secondary star formation in the shell as well as the timescales involved, indicate a possible scenario for triggering star formation, signified by the 'collect and collapse' process.</P>

Promotional effect of Ce in NH3-SCO and NH3-SCR reactions over Cu-Ce/ SCR catalysts

Wenjie Liu,Yifei Long,Shinian Liu,Yongyan Zhou,XinTong,Yajie Yin,Xiaoyi Li,Kang Hu,Jiangjun Hu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-

Commercial V-W-Ti catalysts were modified with Cu and different contents of Ce to remove slip ammoniaand remaining nitrogen oxide from the end stage of SCR technology. The activities of NH3 oxidation byO2 (NH3-SCO) and NO reduction by NH3 (NH3-SCR) of these catalysts were examined from 150 to 400 ℃. The consequences indicated that the addition of Ce and Cu could considerably enhance NH3 oxidation andNO reduction capacity of the catalysts and Ce was conducive to reduce the detrimental effects of H2O andSO2. The characterization tests indicated that the surface active oxygen increased because of the interactionbetween V, Cu and Ce. Moreover, the loaded Ce and Cu contributed to enhance the surface acidityand redox property of SCR catalysts.

Transcriptomic analyses reveal novel genes with sexually dimorphic expression in Takifugu rubripes brain during gonadal sex differentiation

Xufang Shen,Hongwei Yan,Lei Zhang,Zhen Yuan,Wenlei Liu,Yumeng Wu,Qi Liu,Xiaoyi Luo,Ying Liu 한국유전학회 2020 Genes & Genomics Vol.42 No.4

Background Quantification of mRNAs in gonads and other tissues at the early critical development stage of sex differentiation may help to provide a global view of regulatory mechanisms underlying sex differentiation. We have recently reported the transcriptomic profiling of fugu gonad associated with sex differentiation. Objectives This study attempted to identify the genes in the brain that are involved in gonadal differentiation and development. Methods In this study, a transcriptomic scan of potential candidate genes involved in sex differentiation was conducted in the brains of fugu larvae at 30 and 40 dah (morphological gonadal sex differentiation had not yet occurred). The dimorphic expression patterns of several candidate genes were verified using quantitative PCR. Results A total of 28.24 Gb of clean reads were obtained and 22,337 genes were identified in the brains of fugu larvae. These included 1008 novel genes that provide abundant data for functional analysis of sex differentiation. 229 genes were identified in the 30 dah larvae that were abundant in the XY brain and 21 that were abundant in the XX brain. In the 40 dah larvae, 325 genes were identified abundant in the XY brain and 174 were identified abundant in the XX brain. Conclusion This is the first investigation into the transcriptome of the fugu larvae brain at the early sex differentiation stage. The results obtained here will enhance the understanding of molecular mechanisms that underly fugu sex differentiation.

MOPSO-based Data Scheduling Scheme for P2P Streaming Systems

( Pingshan Liu ),( Yaqing Fan ),( Xiaoyi Xiong ),( Yimin Wen ),( Dianjie Lu ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.10

In the Peer-to-Peer (P2P) streaming systems, peers randomly form a network overlay to share video resources with a data scheduling scheme. A data scheduling scheme can have a great impact on system performance, which should achieve two optimal objectives at the same time ideally. The two optimization objectives are to improve the perceived video quality and maximize the network throughput, respectively. Maximizing network throughput means improving the utilization of peer’s upload bandwidth. However, maximizing network throughput will result in a reduction in the perceived video quality, and vice versa. Therefore, to achieve the above two objects simultaneously, we proposed a new data scheduling scheme based on multi-objective particle swarm optimization data scheduling scheme, called MOPSO-DS scheme. To design the MOPSO-DS scheme, we first formulated the data scheduling optimization problem as a multi-objective optimization problem. Then, a multi-objective particle swarm optimization algorithm is proposed by encoding the neighbors of peers as the position vector of the particles. Through extensive simulations, we demonstrated the MOPSO-DS scheme could improve the system performance effectively.

Mobile Manufacturer or Service provider? An Empirical Study on Consumers’ Adoption Intention

Feng Hu,Xiaoyi Du,Yong Liu 보안공학연구지원센터(IJUNESST) 2010 International Journal of u- and e- Service, Scienc Vol.3 No.2

Exploring the Creeping Landslide Characteristics Based on the Time Series InSAR in the Xianshuihe Fault Zone, Southwest China

( Zhihua Yang ),( Xiaoyi Liu ),( Changbao Guo ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

The Xianshuihe Fault in the southwest China presents strong activities Since the Holocene. Under the joint action of strong earthquakes, fault activities and heavy rainfall, the landslides have shown high development intensity in the Xianshuihe fault zone and bring a serious threat to the safety of human life and property. In this study, the SBAS-InSAR method and ALOS PALSAR data (15 scenes) are used to calculate and analyze the deformation characteristics of the creeping landslide between 2007 and 2011. The results show that the active effect of the Xianshuihe Fault has an important influence on the landslide development and occurrence. The closer distance to the Xianshuihe Fault is, the more stable coherent points is, the greater landslide cumulative displacement is. A total of 98 creeping landslides in the Xianshuihe Fault zone are identified, which develop as a linear distribution on both sides of the Xianshuihe Fault. And, the surface deformation and development characteristics of several typical creeping landslides are analyzed in-depth. So, the InSAR method can be used to effectively analyze the slow surface deformation, change laws and development characteristics of creeping landslide in the active fault zone. The study results have a certain guiding role on the landslide prevention and mitigation along the Xianshuihe Fault zone and the similar tectonic activity area.

Ultrafast dynamics control on ablation of Cu using shaped femtosecond pulse trains

Deng Jiannan,Qi Hongxia,Liu Xinyi,Li Xiaoyi,Tong Qiunan,Lian Zhenzhong,Li Juan,Bo Jinqiu,Fei Dehou,Chen Zhou,Hu Zhan 한국물리학회 2021 Current Applied Physics Vol.26 No.-

The ablation processes of Cu film are investigated using temporal shaped femtosecond pulse trains. The depth is modulated by changing the number and interval of the sub-pulses. The underlying ultrafast dynamic processes are discussed based on plasma shielding and electron multiple heating mechanisms. When the sub-pulse interval is less than 0.4 ps electron multiple heating is the dominant mechanism, while the plasma shielding dominates the subsequent ablation processes when the sub-pulse interval is larger than 0.4 ps. The curve of depth obtained by three pulse trains shows more significant oscillation as the function of sub-pulse interval under the lowfluence. We propose that the oscillation of depth is due to the coherent phonon oscillation excited by the pulse train. The study provides a basis for giving insight into the ultrafast dynamics for improving micromachining and nano-fabrications using shaped femtosecond pulse trains.

Antifreeze protein detection using Rhodamine B as photoluminescence label in porous silicon

Hongyan Zhang,Zhenhong Jia,Xiaoyi Lv,Junwei Hou,Xiaojing Liu,Ji Ma,Jun Zhou 한국물리학회 2013 Current Applied Physics Vol.13 No.4

A novel method is demonstrated to detect Antifreeze proteins (AFPs) based on photoluminescence (PL)using porous silicon (PS) coated with silver as a substrate. Ag/PS substrate is obtained through immersion of PS in silver nitrate (AgNO3) solutions and is incubated with Rhodamine B (RB) as PL label. This substrate is easy to be fabricated and the pore size of PS is large enough for biological molecules to infiltrate, which is an ideal platform for biological molecule detection. Through functionalization used glutaraldehyde (GTA) and 4-(N-Maleimidomethyl) cyclohexane-1-carboxylicacid (Sulfo-SMCC) as crosslinkers separately, we test the role of the AFPs antibodies in selective capturing the AFPs antigen and explain the reason of the enhancement of PL intensity. The result shows a significant enhancement of the PL intensity of RB at around 590 nm due to the interaction of antibodyeantigen competitive binding with AFPs. Therefore, the PL corresponding to RB was selected to detect the target AFPs and the PL intensity of RB proportional to the AFPs concentration. The detection limit was found to be 1.65 mg/ml for AFPs when GTA was used as cross-linker, and the detection limit was 16.5 ng/ml with Sulfo-SMCC as cross-linker

유도 기원설 진원윤(陳元贇)을 통한 중·일 무술교류 비교연구

차윤선(Cha, Yoon-Sun),LIU XIAOYI,조성균(Cho, Sung-Kyun) 한국무예학회 2022 무예연구 Vol.16 No.4

본 연구는 중국과 일본에서 이루어진 진원윤과 관련된 실제적인 문헌분석을 통하여 유도 기원설에 대한 진위를 파악하고, 유도의 근대적 발전과정에서 진원윤의 영향력에 대해 연구를 위한 기초자료를 제공하는 데에 그 목적이 있다. 연구방법은 문헌고찰을 통해 기술된 자료를 정리하고 해석하여 다음과 같은 결론을 얻었다. 첫째, 유술의 시조설에서 공통된 점은 유술을 에도시대에 확립된 새로운 유형의 격투기로 정의하고 있는 점이다. 이 부분에 대해서는 일본 고유의 무술이었던 스모에서 비롯되어 시대적 배경에 따라 격투술의 모양새를 갖다가 에도시대에 각 유파가 등장한 것으로 해석할 때에 왕도원(王道元) 시조설을 제외한 각 시조설은 나름대로의 유파에서 원조격에 해당한다고 할 수 있다. 둘째, 진원윤은 1587년 중국 절강성 여항현에서 명문가의 아들로 태어난 중국학자이며, 1626년에 일본에 가서 중국 권법을 전수한 것으로 알려져 있다. 그의 생애사를 살펴보았을 때 무인보다는 문인의 삶을 영위했으며 무도보다는 예술과 한문에 뛰어났음을 알 수 있었다. 셋째, 지금까지 대부분의 진원윤과 관련된 선행연구를 살펴보면 실증적인 사실에 입각했다기 보다는 구전과 단순 인용에 근거한 재생산 자료들이 많아 실질적으로 그가 과연 권법에 얼마나 정통했으며 구체적으로 어떤 영향을 주었는지 등의 자료를 찾아보기가 어려웠다. 이러한 점에 미루어보았을 때, 일본 유도사에서 진원윤의 존재는 유술발전 과정의 중요한 시기에 등장한 근대 유술의 중흥의 조력자였다고 볼 수 있다. In order to understand the development process and origin of Judo, this research compares Chinese and Japanese materials about “Chen Yuan-Yun”, a Chinese scholar who is said to have had a great influence on the development process of Jujutsu. The purpose of this study is to understand the truth of the founder theories Judo through the analysis of documents related to him conducted in China and Japan, and to provide basic materials for studying the influence of him in the development process of Judo. After comparing the materials so far, we were able to obtain the following conclusions. First, what is common to the founder theories of Jujutsu is that Jujutsu is defined as a new form of martial arts established in the Edo period. However, considering that Sumo wrestling, which was a martial art unique to Japan, developed into a combative martial art through the changes of the times, and then, and that various schools were born on the Edo period, it can be said that each founder theory is worthy of being the originator of each school. Second, Chen Yuanyun was a Chinese scholar born in 1587 as the son of a prestigious family, and went to Japan in 1626. And it is said that he taught Chinese martial arts there. Looking back on his life, we find that he lived a life of a literary artist rather than a martial artist. Third, most of the research that has been done on him so far is not based on proven facts, and much of the material has been reproduced by word of mouth or simple quotations. As a result, I have found very little documentation on how proficient he was in martial arts and specifically what kind of influence he had on Jujutsu. Therefore, it can be said that Chen Yuanyuns position is the assistant of the modern Jujutsu revival, which appeared in an important period of development of Judo.