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Antifreeze protein detection using Rhodamine B as photoluminescence label in porous silicon
Hongyan Zhang,Zhenhong Jia,Xiaoyi Lv,Junwei Hou,Xiaojing Liu,Ji Ma,Jun Zhou 한국물리학회 2013 Current Applied Physics Vol.13 No.4
A novel method is demonstrated to detect Antifreeze proteins (AFPs) based on photoluminescence (PL)using porous silicon (PS) coated with silver as a substrate. Ag/PS substrate is obtained through immersion of PS in silver nitrate (AgNO3) solutions and is incubated with Rhodamine B (RB) as PL label. This substrate is easy to be fabricated and the pore size of PS is large enough for biological molecules to infiltrate, which is an ideal platform for biological molecule detection. Through functionalization used glutaraldehyde (GTA) and 4-(N-Maleimidomethyl) cyclohexane-1-carboxylicacid (Sulfo-SMCC) as crosslinkers separately, we test the role of the AFPs antibodies in selective capturing the AFPs antigen and explain the reason of the enhancement of PL intensity. The result shows a significant enhancement of the PL intensity of RB at around 590 nm due to the interaction of antibodyeantigen competitive binding with AFPs. Therefore, the PL corresponding to RB was selected to detect the target AFPs and the PL intensity of RB proportional to the AFPs concentration. The detection limit was found to be 1.65 mg/ml for AFPs when GTA was used as cross-linker, and the detection limit was 16.5 ng/ml with Sulfo-SMCC as cross-linker
Hongyan Zhang,Zhenhong Jia,Xiaoyi Lv 한국물리학회 2015 Current Applied Physics Vol.15 No.8
A technique is demonstrated to detect DNA hybridization based on surface layer of Au/porous silicon microcavity (Au/PSM) substrate for very small amount of biomolecules. Simulations show that the increase of effective refractive index for the first layer of PSM will cause a blue shift for its reflectance spectrum, and the blue shift becomes less with the increase of refractive index for one more layers. In experiments, such a blue shift of reflectance spectrum of PSM comes from the increase of refractive index by DNA hybridization on the surface. The detection limit of Au/PSM biosensor is 15.15 nM for 19-base pair DNA, which is comparable to that of reported biosensors based on porous silicon (PS). Therefore such an Au/PSM could be very useful to develop simple, rapid and sensitive optical biosensors when the amount of target is very small.
Hongyan Zhang,Changqing Bai,Yijun Mao 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.8
Random properties and random loads are highly important in rotor dynamic analysis because they cause system dynamic responses tobehave randomly. In this paper, a stochastical finite element of rotating shaft based on Timosheko beam theory is proposed for rotor systemmodeling, in which material and geometric random properties are considered one-dimensional stochastic field functions. A randomresponse analytical method is developed to determine the statistics of the dynamic responses of stochastical rotor systems under randomloads. The numerically obtained whirl speed of a turbopump rotor system is compared with the test data to validate the proposed model,and good agreement is observed. Linear and nonlinear turbopump rotor systems are employed to compare the results obtained from theproposed model and the Monte Carlo simulation. The numerically predicted results, which coincide well with Monte Carlo simulationdata, demonstrate the feasibility and efficiency of the proposed stochastic model and method for actual rotor system analysis and design.
Hongyan Zhang,Shoko Hosoi-Tanabe,Shinichi Nagata,Syuhei Ban,Satoshi Imura 한국미생물학회 2010 The journal of microbiology Vol.48 No.2
A novel Gram-negative, aerobic, moderate halophilic, and psychrotolerant bacterium, designated as strain H7T, was isolated from a hypersaline lake located in Skarvsnes, Antarctica. Cells were filaments with varying lengths. Coccoid bodies developed in old cultures. Growth occurred with 0.5-15% (w/v) NaCl (optimum, 5.8-7.0%), at pH 6.0-10.0 (optimum, pH 7.0-8.0), and at 10-28°C (optimum, 25°C). The strain had a G+C content of 34.9 mol%, which is within the range of 32-36 mol% reported for the genus Psychroflexus. Chemotaxonomic data (major respiratory quinone: MK-6; major fatty acids: aC15:0, iC16:0 3-OH, and aC15: 1 A) supported the classification of strain H7T within the genus Psychroflexus. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain H7T should be assigned to the genus Psychroflexus and has a homology with Psychroflexus salinarum (98.2%), P. sediminis (96.1%), P. torquis (95.2%), P. tropicus (95.8%), and P. gondwanense (92.2%). Strain H7 is not identified as P. salinarum because that DNA-DNA hybridization data were 8.5% between strain H7T and P. salinarum. The combination of phylogenetic analysis, DNA-DNA hybridization data, phenotypic characteristics, and chemotaxonomic differences supported the view that strain H7T represents a novel species of the genus Psychroflexus. The name Psychroflexus lacisalsi is proposed,and the type strain is H7T (=JCM 16231T =KACC 14089T).
Zhang Ling,Zhang Hongyan,Chen Cao,Hu Ziyan,Wang Jun 한국물리학회 2023 Current Applied Physics Vol.45 No.-
Based on ZnO and WO3⋅H2O composite (ZW), a high performance ammonia (NH3) gas sensor was synthesized by hydrothermal method. Compared with WO3⋅H2O, ZW sensor has good selectivity and sensitivity for NH3 gas detection at room temperature, with fast response/recovery time of 2.4 s/1.2 s, low detection limit of 0.76 ppm, excellent endurance to humidity and good stability for a long time. With smaller size in it, ZW provides more active sites to absorb NH3 molecules. More free oxygens on surface of ZW will react with more NH3 molecules, thus to improve the sensitivity of ZW. Furthermore, the heterojunction structure of ZW compound can enhance resistance modulation at the interface between ZnO and WO3⋅H2O, which further improves the sensitivity of WO3⋅H2O for NH3 detection. This study shows the combination of ZnO can significantly improve the performance of NH3 gas sensor based on WO3⋅H2O at RT.
Ping Hongyan,Wang Degang,Fu Maxian,Chen Kaihong,Zhang Jiuhong,Li Ke,Jiang Xuewu,Duan Shouxing,Zhang Xuan 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.4
Background Diethylstilbestrol (DES) has been shown to disrupt the morphology and proliferation of gubernaculum testis cells. Objective This study aimed to elucidate the mechanism underlying DES induced damage to gubernaculum testis based on proteome analysis. Results Neonatal mice were exposed to DES or control vehicle and the gonads were collected for isobaric tags for relative and absolute quantitation based proteomics analysis. We found that at the early and late stages of gubernaculum development, treatment with different concentrations of DES upregulated the expression of proteins which were generally involved in several function categories including single-organism process, cellular process, binding, catalytic activity, cell part and organelle. KEGG pathway analysis showed that cardiac muscle contraction, oxidative phosphorylation, calcium signaling and cGMP-PKG signaling pathway were the most dysregulated pathways associated with DES in the late stage of gubernaculum development, while steroid biosynthesis was the only enriched pathway in the early stage of gubernaculum development. Conclusion Proteomics profi ling and functional analysis reveal new clues on how DES interferes with cellular processes and disrupts the development of gubernaculum. These findings have potential applications in the treatment of genitourinary diseases such as cryptorchidism.
Dust-ion Acoustic Shock Waves in a Dusty Multi-ion Plasma with Negatively Dust-charge Fluctuation
Hongyan Wang,Kaibiao Zhang 한국물리학회 2015 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.66 No.2
The nonlinear propagation of dust-ion acoustic shock waves in a collisionless, unmagnetizedmulti-ion dusty plasma contains Botlzemann-distributed electrons, negative and positive ions withextremely massive and stationary negative charge dust grains with dust charge fluctuations is investigated. By employing the reductive perturbation method, we obtain a Burgers equation thatdescribes the two-ion fluid dynamics. The dust charge variation is found to play an important rolein the formation of such dust-ion acoustic shock structures. The viscosity only affects the thicknessof the shock waves. The dependences of the shock wave’s velocity, height and thickness on thesystem parameters are investigated.