녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

저질에 따른 바지락(Ruditapes philippinarum)의 염분내성

한경호,오성현,장선익,이우범,이원교 國立 麗水大學校 環境問題硏究所 2000 環境硏究論文集 Vol.3 No.-

Salinity tolerance of the manila clam, Ruditapes philippinarum in Gang-jin Bay on the from May to June was investigated in two different types of sedimentary composition. In the experimental groups of sandy-mud, the survival and infiltration rate were good at 20~40ppt, but all the manila clam, Ruditapes philippinarum died at more than 50ppt and less than 10ppt before the experiment finish. In the experimental groups of muddy, the survival and infiltration rate were the best at 25~35ppt and then 20ppt, 40ppt. All the manila clam, Ruditapes philippinarum died at more than 45ppt and less than 15ppt. The survival rate in the experimental groups of sandy-mud was higher than muddy ones, and the infiltration rate was the highest at 20~35ppt in the both of two experimental groups. In the sand-mud, the manila clam, Ruditapes philippinarum infiltrated half of their shells but in the muddy, they infiltrated more then half of their shells.

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

정전 열 접합을 이용한 Multi-Substrate Bonding

이덕중,주병권,최우범,한정인,조경익,이광배,장진,오명환 경북대학교 센서기술연구소 1997 센서技術學術大會論文集 Vol.8 No.1

We performed silicon-to-glass bonding using silicon direct bonding followed by anodic bonding(SDAB). Initial bonding between glass and silicon was caused by the hydrophilic surfaces of silicon and glass ensemble using silicon direct bonding(SDB) method. We found that the bonded specimen using SDAB process had higher strength than one using anodic bonding process only. We performed multiple layer bonding by SDAB, which is glass- silicon -glass as sandwich structure. In the silicon wafer, the (1mm x 2mm x 500μm)-sized cavity was formed by the anisotropic etching of the silicon substrate in EPW(Ethylendiamin-Pyrocatechol-Water). And, the cavity was sealed with glass wafers by SDAB method.

한국어 판 구조화 임상면담도구 개발 : 신뢰도 연구

한오수,안준호,송선희,조맹제,김장규,배재남,조성진,정범수,서동우,함봉진,이동우,박종익,홍진표 대한신경정신의학회 2000 신경정신의학 Vol.39 No.2

연구목적: 정신장애의 정확한 진단과 평가는 임상에서뿐만 아니라 신뢰성 있는 연구를 위해서도 매우 중요하다. Structured Clinical Interview for DSM-IV(SCID)는 임상전문가들이 사용하는 진단도구로서, 비교적 짧은 시간에 정확한 진단을 내릴 수 있다. 본 연구는 한국어판SCID를 개발하고 그 신뢰도를 평가하기 위해 수행되었다. 방법: 국문학자가 포함된 번역위원회를 통하여 연구용판 SCID를 번역한 후, 정신과 의사 2인과 임상심리학자 1인에게 한국어판SCID 실시방법을 교육시킨 뒤 한국어판SCID를 이용한 면담의 평가자간 신뢰도 (interrater reliability)를 검증하였다. 면담 대상은 1999년 2월에서 3월까지 2개 병원 정신과에 치료중인 환자 90명(남:41명, 여:29명)이었다. 결과: 현재 장애(current disorder) 평가에서 주요 우울장애, 기분부전장애, 정신분열병, 알코올 남용 및 의존, 기타 물질 남용 또는 의존, 여러 불안장애들 및 섭식장애 등과 같은 대부분 장애의 kappa값은 .70이상으로 매우 높았다. 그 이외의 양극성 장애, 망상장애, 광장공포증, 감별 불능 신체화 장애 및 건강염려증의 kappa값도 .69에서 .40사이로 수용할 수 있는 정도였다. 평생 장애(lifetime disorder)에서는 양극성 장애(k=.69)와 감별 불능 신체화장애(k=.59)를 제외한 다른 모든 장애의 kappa값이 .70이상이었다. K-SCID 면담시 Ⅰ축 질환에 대하여 흔하게 다중 진단이 내려졌으며, 평균 진단 수는 1.5∼1.7개로 나타났다. 결론: 한국어판 SCID는 신뢰도가 높은 진단도구로 생각되며, 향후 정신질환의 정확한 진단과 임상연구에 유용하게 사용될 수 있다. Objectives: Accurate diagnosis and assessment for psychiatric disorders is crucial for research, as well as for clinical practice. Structured Clinical Interview for DSM-Ⅳ(SCID-RV) is a less time-consumimg and more accurate structured diagnostic interview form. It can be used by clinical professions and is known for a reliable diagnostic tool. Present study was conducted to develop Korean version of SCID-RV and to test the inter-rater reliability. Methods: The authors have translated original SCID-RV into Korean, and revised in parallel with sociocultural background of Korea. Ninety patients from two psychiatric hospitals, both outpatient and inpatient, were interviewed and rated independently by three raters. Results: The kappa coefficients for most of illnesses, such as major depressive disorder, dysthymia, schizophrenia, alcohol abuse and dependency, anxiety disorder and eating disorder were excellent(>0.70) in the evaluation of current disorders. And the kappa coefficients for bipolar disorder, delusional disorder, agoraphobia, undifferentiated somatoform disorder, and hypochondriasis were acceptable(>0.40) in the evaluation of current disorders. In the evalua-tion of lifetime disorders, the concordant rates of all the diagnoses except bipolar disorder and undifferentiated somatoform disorder were excellent. Lack of hierarchy in DSM-Ⅳ allows for multiple Axis I diagnoses. Mean numbers of Axis I diagnoses per subject assigned by the three raters were 1.5-1.7. Conclusions: Our findings confirm that SCID-RV yields highly reliable diagnoses. SCID-RV is recommended for accurate diagnosis in clinical practice and research on psychiatric disorders.

An optically efficient full-color reflective display with an electrochromic device and color production units

Ik Jang Ko,Jin Hwan Park,Gyeong Woo Kim,Raju Lampande,Jang Hyuk Kwon 한국정보디스플레이학회 2019 Journal of information display Vol.20 No.3

In this paper, we report a full-color reflective display device by combining reflective electrochromic device (ECD) and different color production units (conventional color filter (CF) and transmittance controllable electrochromic color filter (TCECF)). A full-color reflective device with TCECF showed an excellent diffuse reflectance of 47.2% in the white state owing to the high transmittance of TCECF in the bleached state than that of CF (19.5%). This device structure can easily provide various colors with high brightness and saturation with a broad grayscale. Particularly, in the colored state, TCECF and CF-based full-color reflective device displayed color coordinates of (0.59, 0.34), (0.31, 0.66), (0.24, 0.30) and (0.65, 0.33), (0.27, 0.61), (0.15, 0.06), for red, green and blue, respectively, and alongside also exhibited color gamut of 36.8% (for TCECF) and 73.1% (for CF) for full color reflective devices.

웹 기반 가상 현실 스마트 팜 서비스

장우석(Woo-seok Jang),오승택(Seung-taek Oh),이동익(Dong-ik Lee),진성호(Sung-ho Jin),안형태,오병우(Byung-woo Oh) 한국정보기술학회 2022 Proceedings of KIIT Conference Vol.2022 No.6

전 세계적으로 채식의 수요량이 증가함에 따라 다양한 공간에서 식물을 재배할 수 있는 스마트 팜 기술이 주목받고 있다. 스마트 팜 운영이 활성화되며 스마트 팜의 관심이 높아졌으나 개인이 신기술이 적용된 농업에 대한 정보를 입수하기에 어려움이 크다. 본 논문에서는 스마트 팜 구축을 위한 개인들을 대상으로 반응형 웹 기술과 WebGL 기술을 활용하여 가상공간에서 사용자에게 스마트 팜 운영에 필요한 설비와 식물의 생장에 필요한 요소에 대한 정보를 제공하고 이를 시험해 볼 수 있는 서비스를 제안한다. As the demand for vegetables increases around the world, smart farming technology that can grow plants in various spaces is drawing attention. Although smart farming have been activated and interest in smart farms has increased, it is difficult for individuals to obtain information on agriculture with new technologies. In this paper, we propose a service that allows individuals to use reactive web technologies and WebGL technologies to provide users information on the facilities and plant growth necessary for smart farm operation in a virtual space and test them.

Development of an optimal protocol to induce capacitation of boar spermatozoa in vitro

Seung-Ik Jang,Jae-Hwan Jo,Eun-Ju Jung,Woo-Jin Lee,Ju-Mi Hwang,Jeong-Won Bae,Woo-Sung Kwon The Korean Society of Animal Reproduction and Biot 2022 한국동물생명공학회지 Vol.37 No.4

In 1951, Colin Russell Austin and Min Chueh Chang identified "capacitation", a special process involving ejaculated spermatozoa in the female reproductive tract. Capacitation is a phenomenon that occurs in vivo, but almost all knowledge of capacitation has been obtained from in vitro studies. Therefore, numerous trials have been performed to establish in vitro capacitation methods for various studies on reproduction. Although a series of studies have been conducted to develop an optimal protocol for inducing capacitation, most have focused on identifying the appropriate chemical compounds to induce the capacitation of boar spermatozoa in vitro. Therefore, the purpose of this study was to identify the optimal incubation time for inducing capacitation in vitro. Duroc semen was incubated for various periods (60, 90, and 120 min) to induce capacitation. Sperm function (sperm motility, motion kinematic parameters, and capacitation status) was evaluated. The results showed that total sperm motility, rapid sperm motility, progressive sperm motility, curvilinear velocity, and average path velocity significantly decreased in a time-dependent manner. However, the capacitation status did not show any significant changes. Taken together, these results indicate that an incubation time of more than 60 min suppresses sperm motility and motion kinematic parameters. Therefore, we suggest that 60 min may be the best incubation time to induce capacitation without negative effects on sperm motility and motion kinematics in boar spermatozoa in vitro.

Breeding of Late Bolting and New Dark Red Leaf Lettuce ‘Chunpung’

Suk Woo Jang,Youn Young Hur,Mi Ja Choi,Young Seok Kwon,Jeom Sun Kim,Jong Nam Lee,Eung Ho Lee,Myeong Hun Seo,Jae Ho Park,Ik Jang,Mi Hyang Jang,Sun Bo Ko 한국원예학회 2010 한국원예학회 학술발표요지 Vol.2010 No.10

Breeding of Late Bolting and High Yield Lettuce ‘Hyesun’

Suk-Woo Jang,Seoung-Ryong Cheong,Dae-Young Kim,Hye-Sun Shin,Jong-Nam Lee,Myeong Hee Cheon,Jae Ho Park,Ik Jang,Hee-Dae Kim,Sun-Bo Ko 한국원예학회 2014 한국원예학회 학술발표요지 Vol.2014 No.5