Disruption of rsmA Gene of Pectobacterium carotovorum subsp. carotovorum LY34 and Effect on Pathogenicity

Kim, Min Keun,Kang, Tae Ho,Kim, Sung Kyum,Jeong, Yu Seok,Yun, Han Dae,Kim, Hoon The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.6

The rsmA gene was cloned from soft-rot bacterium Pectobacterium carotovorum subsp. carotovorum LY34 (Pcc LY34), and its role in pathogenicity was investigated by marker exchange mutagenesis. From a cosmid library of Pcc LY34 genomic DNA, a positive clone carrying the rsmA gene was selected, and the gene was cloned by polymerase chain reaction (PCR) amplification. The gene is 186 bp in size and encodes a protein of 62 amino acids with a predicted molecular mass of 6,839 Da. The calculated pI of the RsmA is 8.16. The phylogenetic tree showed that the RsmA of Pcc LY34 appeared genetically identical to the CsrA of Pectobacterium atrosepticum SCRI1043 (100% identity) and similar to the CsrA of Yersinia pestis KIM10+(98.3%). The gene was disrupted by the $Km^r$ gene, and the cells became mutated (i.e., $RsmA^-$ mutant). The pathogenicity test revealed that the disease rating of the $RsmA^-$ mutant only differed slightly from that of the wild type on a slice of potato tuber and a Chinese cabbage stalk. These results suggest that RsmA is not an essential factor for the pathogenicity of Pcc LY34 and that the rsmA gene of Pcc LY34 is not completely derepressed in the $RsmA^-$ mutant for virulence-related genes, contrary to the results of Erwinia carotovora subsp. carotovora $RsmA^-$ mutant, which proved hypervirulent for celery petioles. These results showed that the microenvironmental conditions of the host and/or strain of pathogen are important for the coordination of virulence gene expression.

Proteomic Approach to Analyzing Flooding Stress in Soybean (Glycine max(L.) Merr.)

Kim,Tae-Seon,Cho,Seong-Woo,Lee,Jae-Eun,Kim,Yook-Han,Cho,Kun,Choi,Jong-Soon,Chung,Keun-Yook,Song,Beom-Heon,Jong,Seung-Keun,Kim,Hong-Sig,Woo,Sun-Hee 한국작물학회 2005 한국작물학회 학술발표대회 논문집 Vol.2005 No.-

Misorientation Characteristics at the Growth Front of Abnormally-Growing Goss Grains in Fe–3%Si Steel

Tae‑Young Kim,Tae‑Wook Na,Hyung‑Seok Shim,Yong‑Keun Ahn,Yong‑Kwon Jeong,Heung Nam Han,Nong‑Moon Hwang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

Penetrating morphologies at the growth front of abnormally-growing Goss grains in Fe–3%Si steel was examined in view ofthe possibility that they are the two-dimensional section of the solid-state wetting (SSW) along the triple junction line. Toobtain the statistically meaningful data, 102 penetrated grain and 204 penetrating abnormally-growing Goss grain morphologieswere examined. The misorientation angles of penetrating and penetrated grains were measured by electron backscattereddiffraction. Among the 102 examined penetrated grain boundaries, none has low misorientation angles less than 15°,whereas 17.2% of the 204 penetrating grain boundaries have low misorientation angles, and 23.5% of them have coincidencesite lattice boundaries. Besides, boundary energies of penetrating Goss grains, which were estimated from misorientationangles of the three grains in the penetrating morphology, satisfied the energetic condition for SSW along the triple junctionline. These results imply that the abnormal grain growth of Goss grains in Fe–3%Si steel occurs by the mechanism of subboundaryenhanced SSW.

목재가 선적된 선창에서 발생한 산소결핍에 의한 질식사

김동훈,김기권,장태정,김정란,이구,김수근,임현술 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

Oxygen deficiency has been frequent in a clouted space. Wood consumes oxygen and discharges carbon dioxide Instead of photosynthesis in closed space without light, so do some microorganisms on the surface. We experienced a case that a healthy Insect-proofer fell down and died of asphyxia on stair-board at 7 m below the hatch of the cargo-hold shipping wood Analysis of gases in cargo-hold revealed O2; 12.3%, CO; 105 ppm, CH4; 2.7%, and H2S; 1.9% at 1 m below the hatch, and then O2; 6.1%, CO; 220 ppm, CH4; 2.9%, and H2S, 2.3 ppm at 2.5 m below the hatch. Autopsy findings were unremarkable. We justiced the cause of death asphyxia due to oxygen deficiency. As seen in this case, the serious oxygen deficiency was accounted for oxygen consumption by wood and microorganisms.

AHP를 이용한 CT 및 MRI의 비용편익분석

조근태,김성조,김성민,김용우,김성재,조용곤 한국경영과학회 2004 經營 科學 Vol.21 No.2

The imports of used medical equipment such as CT, MRI have been rapidly increased every year. How to manage those medical equipment has been one of critical issues in the government sector. In this study, we demonstrate how benefit/cost analysis using the Analytic Hierarchy Process (AHP) can be applied to the comparison of used CT/MRI and new CT/MRI. The final results show that the new CT/MRI is more attractive than the used CT/MRI.

Pollen Viability of Common Buckwheat (Fagopyrum esculentum Moench.) in in vitro Germination and its Application to Male Gametophyte Isolation

Woo, Sun-HeeㆍKeun-Yook ChungㆍHeung-Tae, KimㆍYong-Gu, ChoㆍHong-Sig, KimㆍBeom-Heon, Songㆍ Chul-Won, Lee and Seung-Keun Jong 忠北大學校 農業科學硏究所 2004 農業科學硏究 Vol.21 No.-

-본문참고-

벼 大單位 增産團地栽培에 關한 經營實態調査 : 慶南地方의 5個 團地를 中心으로

金厚根,金正敎,尹泰圭,河湖成,張權烈 慶尙大學校 1975 論文集 Vol.14 No.-

Recently large scale cooperative rice production system has been promoted. This cooperative rice production system is intended to promote introducing a set of new technology which may not be suitable for individual small scale farms to adopt. And this system is believed to be one of important public programs to achieve productivity growth policy goal in Korea. Nevertheless, there seem to exist several problems for this public program to widely introduce to over-all nation. This is one of main objectives that this study intends to investigate. In order to study comparative characteristics of the large scale rice cooperative farm in terms of resource base and organization 99 farms which are member farms and 100 farms which are non-member individual farms have been selected and interviewed from five large scale cooperative farm areas in Gyeong-Nam Province in 1974. The important findings can be summarized as follows: 1) Most of operators af both classes belong to age cohert 40∼50, but the average educational level for member farm operators is higher than that for non-member. 2) The average scale Of rice paddy for member farms, 13.1 tanbO, is larger than that for non-member, 10.6, and the same thing is true for average size of rice paddy plots which is, respectively, 805 pyeong and 690. 3) Completely irrigated paddy for member farms is 99 percent of total paddy owned whereas that for non-member is 81, and consolidated paddy for member farms is 67 percent while that for non-member is 26.4. On the other hand, technically improved paddy other than indicated above for member farms is 32 percent, but that for non-member is only 8.3. 4) Number of rice varieties adopted is 9 for both classes. The most popular variety for member farm is Akibare(36%) whereas that for non-member is Sadominori(29.3%) and Akibare (28.4%). 5) Labor used is 13.5 man per tanbo for member farms and 12.4 for non-member. More labor is used for improving soil quality and protection for member farms whereas less labor is used for preparatory works such as seed bed, sterilization, and water management in the case of the member farm. 6) In the case of member farms, the type of work which is achieved cooperatively by more than 70 percent is purchasing or exchaging seeds, sterilization, and plant protection. Despite seed bad, water management, transplanting and the like being more or less suitable for cooperative work, the proportion of those works done cooperatively is relatively small as compared to what we expected. 7) According to farmers interviewed, variety selection, plant protection, seed bed, transplanting fertilization, water management, and harvesting works are more easier for cooperation, in order importance. 8) Cooperative farmers were cultivating a leading variety, Tongil, which showed higher yields than ordinary varieties and had a desire to be released a new high yielding variety which has a characteristics of highly resistance to dsease and insect. 9) Cooperative farmers were forced to begin the nusery bed earlier than the individual farmers and to emphasize on the disease and insect control with more frequencies. 10) Cooperative farmers had benefits in the seedling transplanting earlier and higher planting density than individual farmers. 11) Cooperative farmers had an intention to apply a deep fertilization method arid to dress heavier nitrogen with a reasonable ratio of the basal fertilization to the top dressing. 12) Cooperative farmers had a convenient irrigation system and were available to adopt a summer drainage method. 13) Cooperative farmers controlled the weed with agricultural chemicals arid recognized the control effect of weedcides. 14) In general, it was recognized that there happened somewhat benefit result in disease control in the cooperative farm while a reasonable control effect was not found in heavy prevailing case. The dominant diseases found in the cooperative farm were sheath bright, strip disease and they were to he prevented with a strong efforts. 15) Rice stem borer and grass leaf roller were effelctivey controlled in the cooperative farm, however, plant hoppers was not completely controlled by the usual method because of its unexpected prevalence. 16) The most difficult thing to cooperate they indicate is to supply an adequate amount of labor at appropriate time in tire labor peak season. In this sense, it seems necessary to introduce field machinary in order to achieve the objective of large scale cooperative rice production. 17) Farmers interviewed believe that technology concerning yield increase is most important. in this respect, it is important to develop such new technology, to diseminate its results and to train good quality extension workers. 18) 42 percent of member farm operators indicate that the cooperative system was successful and 25 percent indicate failed. 29 percent of non-member farmers think that the cooperative farm system was successful and 7 percent of them think failed. Thus 55 percent of non-member farmers indicate they are willing to join to the system in the next year, 7 percent of them are not and others have not decided yet. 19) 60 percent of member farmers think that the scale and number of members of the system were too much large to successfully cooperate and 67 percent of them believe that cooperation among members were not very developed, and 42 percent of members suppose what the leader of individual active farms were not very well functioning. 20) Number of member farmers who believe that the yield level in this year is increased as compared that before joining to the system is 3,4 percent, and no one indicated that the yield level is decreased. On the other hand, 22 percent of them believe that by participating to the system the same quality of paddy could produce more rice. 21) About 93 percent of member farmers believe that the system may not lie survived without support of government and i priority must be given to yield technology. 22) About 80 percent of member operators indicate that they would partioipate to the program in the next production year and 23 percent of them indicate that they would participate with the same amount of paddy land as this year. 23) The rice yield level for member and non-member farms is, respectively, 406kg and 346 per tanbo, and the former is 17.3 percent higher than the latter. 24) The cash expenses per tanbo for member and non-member farms is, respectively, 12,311 won and 10,398. 25) The gross revenud per tanbo for both classes is, respectively, 60,024 won, and 51,303 when evaluated by the official price level, and the net income is, respectively, 47,713 won and 40,905. As seen above, the fact that the rice yield level of member farmers, hence, the income level turns out to be higher seems to stem from two basic factors; that is, on the average, the member farmers are operating farm with an improved resource base and they seem to use more the so called conventional inputs with a better yield technology and management. Thus we may conclude that it is important to invest to improve resource quality, to innovate new yield technology and diseminate this innovation. At the same time, in order to more promote the cooperative system, it seems desirable to solve the following problem areas: 1) Number of member farms and paddy area covered by one cooperaitve unit seems better not to he very large. This seems so because there is on much scale economies owing to the nature of technology available to adopt, whereas a large member unit may restrict opportunity of individual members to deeply involve in the process of decision making. Thus we recommend to continue to study finding an optimum size of the unit. 2) As implied already, quality as well as quantity of extension workers and other local officers who lead this program area crucial factor to expand number of unit of the system and hence to achieve the objective of the program. 3) In order to get rid of labor deficit, especially in the labor peak season farm mechanization seems necessary. For this matter, it is worthwhile to promote, first of all, to invent suitable field machinery, secondly, land consolidation and other land improvement projects, and thirdly to innovate a new technology system suitable to mechanized farming. At any rate, it is also necessary for the government to supply an adequate amount of credit and other administrative support.

3상 유도전동기운전에 대한 전압고조파영향의 분석

김태한,좌종근 제주대학교 공과대학 첨단기술연구소 2005 尖端技術硏究所論文集 Vol.16 No.1

In this paper an attempt is made to analyze the time harmonic effects on a three phase induction motor operation. The T-type equivalent circuit is used to calculate the current distortion factor and efficiency of the motor under the nonsinusoidal voltage source which is consisted of the fundamental combined with the 2nd to 13th order harmonic. The current distortion factors and efficiencies are computed and analyzed at various voltage distortion factors with load variation. The results show that the low order harmonics have more impacts on the motor than the higer order harmonics.

초등학교 학생의 가정학습기술의 구성요소에 대한 탐색

김명철,정태근 부산대학교 학생생활연구소 2001 學生指導硏究 Vol.20 No.-

There has been going on a dispute about the effectiveness of homework. There were a lot of results from studying about it but researchers have been from unanimous. So for the study particularly has been performing about quantity of homework and the time of homework, parental involvement, teacher reinforcement and feedback, attitude, the kinds of homework, the grade level, sex, and so on, but no study on the effect of homework skill on homework performance(homework completion and homework quality) and achievement has been investigated. Before study on it, I felt a need to study about a definition and components of homework. For this study I looked over the study skill and the program of Byun Chang Gin, Kim Seong Hoi, Mun Su Back(1994), Jun Hoa Chun(1998), Kim Eun Ju(1998) developing through an analysis and a synthesis of study skill within and without the country, and various strategies, programs and guides connected with homework, factors influencing the effect of homework, homework style and behavior scale. Through this analysis homework skills was divided into skills for managing school materials, skills for maintaining a home study area, skills for following directions, time management skills, and self―management skills. In future, We need a study to examine that scale development of homework skills, and the extent of that homework skills correlate with the effectiveness of homework, that is homework performance and academic achievement.

유도경기상황의 심판의식 및 판단장애에 관한 연구

김관현,조용철,조문근,김태훈 龍仁大學校 武道硏究所 2002 武道硏究所誌 Vol.13 No.1

The purpose of this study is to produce fair judo judges by examining and analyzing the judicial consciousness during the authentic judo games as well as to attribute to consistent and clear judgment. In accordance with the result of the study I suggest: First, we must try to make judo judges participate continuously in the training program for judo judges and offer them some helpful programs to judge modern judo which is changing right now and to adapt to the new skills or the changed judicial rules so that they may enhance their accurate positions. Second, the judo judges are required to perceive their concrete judicial criteria by repeated practice considering when to give the positive scores and when to give the negative scores after being given the information. Third, more organized training project is needed especially before judo competition, in addition, simulated judicial practice by analyzing some real games on video is helpful to relieve the judo judges' anxiety. Fourth, the judo judge who commited misjudgment or made mistakes on account of his/her circumstances such as his/her academical, regional connections and the teal leaders must be got cold and definite punishment. Lastly, more qualified judo judges, who are distributed fairly in order to be offered more experience are the resources toward he equally high-leveld-judo judges-state. Therefore, we need to study more on the specific elements which influence on the judo judges' judgment and the giving scores.