Porphyromonas gingivalis, Prevotella intermedia, 그리고 Prevotella nigrescens에서의 hemin 결합 단백질에 대한 연구

김성조,Kim, Sung-Jo 대한치주과학회 2006 Journal of Periodontal & Implant Science Vol.36 No.1

The results of this study confirm that the availability of hemin influences the expression of selected membrane proteins of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens. A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is hemin regulated was identified and purified in P. gingivalis. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when P. gingivalis cells were grown under hemin-limited conditions. A 50 kDa cell envelope associated protein, whose expression is hemin regulated, is considered to be a putative hemin binding protein from P. intermedia and P. nigrescens, respectively. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein from P. gingivalis revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins. N-terminal amino acid sequence of a 50 kDa putative hemin binding protein from P. intermedia was identical with Enolase from Streptococcus intermedia. Work is in progress to further characterize the molecular structure of these proteins.

치은퇴축의 처치에 있어 Subepithelial connective tissue graft의 치료효과

김성조 부산대학교 병원 암연구소 1998 부산대병원학술지 Vol.- No.4

치은퇴축의 빈도 및 구강내 분포에 대한 연구

김성조 부산대학교 병원 암연구소 2001 부산대병원학술지 Vol.- No.10

결명자 첨가식이가 흰쥐의 체내 카드뮴 축적에 미치는 영향

김성조,백승화,허종욱,김운성,이주돈,강경원,박성혜,한종현,정성윤,이승현 동아시아식생활학회 2002 東아시아食生活學會誌 Vol.12 No.6

The purpose of this study is to investigate the effect of raw Cassia tora L. powder added-diets on reducing cadmium accumulation in rats. The experimental animals were Sprague-Dawley family(♂ , 4 weeks) which was classified into normal group CN, compared group CS, Cd-added group C1 and groups C2, C3. C4 in which 0.5, 1.0 and 1.5% of the Cassia tora L. powder are added, respectively. The growth rate and food efficiency ratio, and the amounts of accumulated cadmium in rats for 8 weeks were measured and analyzed. The results are as follows; 1. The rates of weight gain decreased in the order of C3>C2>C4>Cn>Cs>C1 groups, and C1 group to which only cadmium water had been fed was the lowest among them. The correlation between groups C1 and C3 was significantly different at the 1% level. 2. Food efficiency ratio(FER) decreased in the order of C3>C2>Cs>Cn>C4>C1, and the FERs of C3, C2, CS, CN and C4 are greater than that of C1 by 22.87, 19.89, 18.54, 14.20 and 13.17%, respectively. 3. As fix the Cassia tora L. powder-added groups, the amounts of cadmium accumulated in organs and tissues that is, the brain, heart, spleen, liver, lungs, testicles, kidney, femoral muscle and leg bones were 0.45±0.04 to 0.83±.04, 1.68±0.02 to 2.16±0.02, 3.26±0.05 to 4.62±0.27, 37.82±0.09 to 47.71±0.73, 1.07±0.10 to 1.66±0.04, 1.04±0.06 to 1.24±0.08, 36.79±0.20 to 39.61±0.53, 0.87±0.02 to 1.00±0.02 and 0.65±0.17 to 1.27±0.06 ㎍/g respectively. 4. The accumulated Cd content for C4 was the lowest among Cassia tora L. powder-added groups. When the results for C4 are compared with those for C1, it is observed that each cadmium content accumulated in the brain, heart, spleen, liver, lungs, testicles, kidney, femoral muscle and leg bones is dropped by 49.03, 22.56, 36.02, 35.75, 41.75, 36.20, 37.00, 22.77 and 56.67%, respectively. On the other hand, the accumulated Cd content increased in the order of brain <leg bones <femoral muscle <testicles <lungs <heart <spleen <kidney <liver. 5. The average Cd accumulation rate in organs and tissues relative to the total Cd intake was 7.14% for C1, while they are 4.91, 4.81 and 4.509a for Cassia tora L. powder-added groups C2, C3 and C4, respectively. 6. The Cd content accumulated in the hair for C1 was the highest, and those for Cassia tora L. powder-added groups gradually increased until 6 weeks and decreased after that. 7. The Cd content accumulated in the feces for C4 was the highest, and those for the groups other than C3 and C4 gradually decreased until 6 weeks and increased after that.

Prevotella intermedia와 Prevotella nigrescens의 세균내독소에 대한 연구;화학적 분석 및 면역생물학적 활성 평가

김성조,Kim, Sung-Jo 대한치주과학회 2004 Journal of Periodontal & Implant Science Vol.34 No.2

The purpose of this study was to assess some biological activities of lipopolysaccharides (LPSs) from P. intermedia and P. nigrescens. LPS was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supernatants. $TNF-{\alpha}$ production was determined by enzyme-linked immunosorbent assay. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. LPS from P. intermedia demonstrated higher KDO content than those from two stains of P. nigrescens. LPSs from P. intermedia and P. nigrescens were mitogenic for spleen cells of BALB/C mouse. The present study clearly shows that LPSs from P. intermedia and P. nigrescens fully induced iNOS expression and NO production in RAW264.7 cells in the absence of other stimuli. Moreover, LPSs from P. intermedia and P. nigrescens clearly induced $TNF-{\alpha}$ production in RAW264.7 cells. The biological activities of LPS from P. intermedia was found to be comparable to those of P. nigrescens LPS. The ability of LPSs from P. intermedia and P. nigrescens to promote the production of NO and $TNF-{\alpha}$ may be important in the pathogenesis of inflammatory periodontal disease.

흰쥐 腦에서의 Cholecystokinin Octapeptide의 分布

김성조,홍해숙,주강 慶北大學校 醫科大學 1986 慶北醫大誌 Vol.27 No.1

흰쥐 뇌에서 cholecystokinin-octapeptide(CCK-8)의 분포를 관찰하기위하여 간접면역형광법을 행하여 관찰해 본 결과 다음의 많은 부위에서 CCK-8양성세포 및 양성섬유를 관찰할 수 있었다. 대뇌피질 : 신피질, 전후핵, 이상엽, 후내야, 해마형성에서 공히 CCK-8양성세포 및 양성섬유가 분포하였다. 중격야 : CCK-8양성섬유가 외측중격핵, n. of accumbens, 분계조의 기저핵, 브로카의 대각핵에서 관찰되었다. 선조체 : n. caudatus putamen에서 CCK-8양성섬유가 존재하였다. 간뇌 : 시상의 경우 전내측시상핵, n. reuniens, n. periventricularis rotundocellularis, 외측슬상체, 내측슬상체에서 CCK-8양성섬유가 관찰되었고 시상하부의 경우 CCK-8양성세포가 시색상핵, 실방핵, 배내측시상하부핵 및 유두상핵에 존재하였고 양성섬유는 내측시속전야, 배내측시상하부 및 복내측시상하부에 분포하였다. 편도체 : CCK-8양성세포는 외측, 기저 및 피질편도핵에서 관찰되었고 양성섬유는 내측, 외측, 기저, 피질편도핵에서 관찰되었다. 중뇌 : 흑질, 복측피개야, n. linea rostralis, 중심회백질, n. parabrachialis colliculi posterioris, 하구 및 외측모대핵에서 CCK-8양성세포가 관찰되었고 양성섬유의 경우 각간핵, n. linea rostralis, 복측피개야, 흑질, 상구, 중심회백질, n. parabrachialis colliculi posterioris, parabigeminal n., 하구, 외측모대핵 및 배측봉선핵에서 관찰되었다. 후뇌 : CCK-8양성세포는 n. parabrachialis lateralis, n. of group O, 고속핵, 최후야 및 삼차신경척수로핵에서 분포하였고 양성섬유는 교봉선핵, 교핵, n. parabrachialis lateralis, 청반핵, n. parolivaris superior, 와우신경핵, 고속핵, 미주신경배측핵, inferior olivary complex, 박속핵, 설상속핵, 삼차신경척수로핵 및 망향체의 일부등에서 존재하였다. In order to investigate the distribution of immunoreactive cholecystokinin octapeptide (CCK-8) containing neurons in the brain of the rat, brains of the rats perfused with fixative were extirpated, frozen sectioned and processed for indirect immunofluorescent technique. The results obtained were as follows. Cortex CCK-8 containing cells and fibers were found in the neocortex, anterior olfactory nucleus (n.) piriform cortex, entorhinal cortex and hippocampal formation. Septal Area CCK-8 containing fibers were present in the lateral septal n., n. of accumbens, basal n. of stria terminalis and n. of diagonal band. Corpus Striatum CCK-8 containing fibers were demonstrated in the n. caudatus putamen. Diencephalon In the anterior medial thalamic n., n. reuniens, n. periventricularis rotundocellularis, lateral geniculate body and medial geniculate body, CCK-8 containing fibers were found. In the hypothalamus, CCK-8 containing cells were present in the supraoptic n., paraventricular n., dorsomedial hypothalamic n. and supramammillary n., and fibers were observed in the medical preoptic n., dorsomedial hypothalamic n. and ventro medical hypothalamic n. Amygdaloid Complex CCK-8 containing cells were distributed in the lateral, basal and cortical n., and fibers were present in the medial, lateral basal and cortical n.. Mid Brain CCK-8 containing cells were found in the ventral tegmental area, substantia nigra, n. linea rostralis, substantia grisea centeralis, n. parabrachialis colliculi posterioris, inferior colliculus and lateral lemmiscus, and fibers were distributed in the interpeduncular n., n. linearostralis, ventral tegmental area, substantia nigra, superior colliculus. substantia gricea centralis, n. parabrachialis colliculi posterioris, parabigeminal n., inferior colliculus, lateral lemniscus, and n. raphe dorsalis. Pons, Medulla Oblongata CCK-8 containing cells were detected in the n. parabrachialis lateralis, n. of group O, n. tractus solitarii, area postrema and n. tractus spinalis nervi trigemini, and fibers were found in the n. centralis superior, pontine n., n. parabrachialis lateralis, locus coeruleus, n. Parolivaris superioris, ocochlear n., n. tractus solitarii, dorsal n. of vagus nerve, inferior olivary complex, n. gracilis, n. cuneatus, n. tractus spinalis nervi trigemini and reticular formation.

Bentazon 連用에 의한 沓多年生 雜草의 防除

金成朝 圓光大學校 1982 論文集 Vol.16 No.2

Bnetazon의 效果的인 使用方法을 究明하기 위하여 劑型, 使用時間, 湛水 및 落水條件 等으로 나누어 連 3年間에 걸쳐 單用, 組合 및 混合處理하여 實驗을 實施하였다. 1. Bentazon의 落水條件下 處理는 너도방동산이, 올방개, 올미 等의 多年生雜草에 對하여 固定된 優秀한 防除效果가 있음을 連用實驗에서 認定되었다. 2. Bentazon은 處理年度에 따라 防除效果에 若干의 變動이 있었다. 3. Bentazon粒劑處理는 移秧 15日後 落水處理가 바람직 하였다. 4. Bentazon單劑 1回 處理로서는 피를 비롯한 一年生雜草의 一部를 防除할 수 없으므로 avirosan 4.4/1.1g, 3kg/10a를 移秧 10日 後에 處理하고 뒤이어 移秧25日後에 Bentazon 48% 液劑 300cc/10a의 處理나 10Gk 3g/10a를 處理하는 것이 3年間의 繼續實驗에서 가장 效果的이었다. To establish the effective weed control methods with bentazon(3-isopropyl-1H-2,1,3,-benzothiadizine-(4)3H-one 2,2-dioxide) in paddy rice, the field experiment was conducted at different formulations, different application time, and flooded and drainage conditions by the continuous use of bentazon alone and /or combination and incorporation with other chemicals during three years. 1. The herbicidal activity of bentazon solution on perennials such as Cyperus serotinus Rottb, Sagittaria pygmaea Mig. and Eleocharis kuroguwai Ohwi was very high when was applied once a year from 1977 to 1979 under drainage condition. 2. The weeding effect of bentazon alone was somewhat shift according to the applied years. 3. Bentazon granule was most desirable when applied 15 days after transplanting(DAT) under drainage condition. 4. As a few annuals uncluding barnyard grass (Echinochloa crusgalli Beav.) cannot be controlled by a single treatment of bentazon granule in the experiments of the continuous use over three years, piperophos+dimethametryn [avirosan;n-(0,0-dipropyl-dithiophosphoryl-acetyl)-2-methyl piperizine+2-methylthio-4-(1,2-dimethyl propyl-amino)-6-ethylamino-s-triazine] 4.4/1.1G at 3kg/10a was most effective on annual and perennial weeds at the time of 10 DAT.

보철 및 Implant와 관련된 치주과 영역의 처치

김성조 대한치과의사협회 1996 대한치과의사협회지 Vol.34 No.10

「SUPERCOMPUTING」특집을 내면서

김성조,유관종,유승화 한국정보과학회 1991 정보과학회지 Vol.9 No.4

포스터 ( 식품 및 미생물 ) : Chlorella 섭취가 Rat 의 납 ( Pb ) 독성에 미치는 영향

김성조,백승화,문광현 한국응용생명화학회(구 한국농화학회) 1996 한국응용생명화학회 학술발표회 Vol.1996 No.1