CD34+ 조혈 모세포 이식 2례

김정아,정현식,김원석,윤성수,이홍기,박찬형,박성규,김동욱,이종욱,한치화,민우성,김춘추,김동집 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

Background: In most solid tumors, the CD34 antigen has not been detected, so positive selection of CD34+ cells may reduce tumor cell contamination and the CD34+ cells are capable of reconstituting hematopoiesis. We tried CD34+ cell transplantation in two patients. Method: CD34+ cells from chemotherapy + G-CSF mobilized PBPCs or bone marrow were positively selected with an avidin-biotin immunoadsorption column (CEPRATE SC system). Case 1. One course of chemotherapy using cyclophosphamide(200㎎/㎡) and etoposide (4.2g/㎡), combined with G-CSF(5㎍/㎏) S.C. was used in a relapsed lymphoma patient. This patient responded to the induction chemotherapy. CD34+ cells from harvested bone marrow were selected by the CellPro immunoadsorption column. The total number of mononuclear cells loaded onto the CellPro was 2.4×10^(8)/㎏, with 1.1% CD34+ cells. After column separation, the total number of positively selected cells was 5.16×10^(6)/㎏. The number of CFU-GM was 76.8×10⁴/㎏. This patient was treated with melphalan (140㎎/㎡) and TBI (1200cGy) and the positively selected CD34+ cells were infused. The time to neutrophil recovery greater than 0.5×10^(9)/L was 19 days and the time to platelet recovery greater than 50×10^(9)/L was 21 days. Case 2. Two courses of mobilizing chemotherapy were given 4 weeks apart using taxol(210㎎/m2) and adriamycin(60㎎/m2), combined with G-CSF(5㎍/㎏) S.C. in a breast cancer patients with 7 axillary node metastasis. CD34+ cells from each single leukapheresis product were selected by the CellPro immunoadsorption column. In the first collection, the total number of nucleated cells was 4.4×10^(8)/kg, with 0.42% CD34+ cells. In the second collection, the total number of nucleated cell was 2.8× 10^(8)/㎏ with 0.43% CD34+ cells. After colum separation, the total numbers of collected cells were 4.0×106/kg and 4.8×10^(6)/kg, the total number of CD34+ cells were 1.2×10^(6)/㎏ and 0.82×10^(6)/㎏. Colonogenic assays of positively selected CD34+ cells gave rise to myeloid erythroid, and multilineage colonies, with a median of 190 CFU-GM, 190 BFU-E, and 164 CFU-GEMM per 1×10³ adsorbed cells, respectively. High-dose chemotherapy with cumulative doses of 40mg/㎡ mitoxantrone, 750mg/㎡ thioptepa, and 1000mg/㎡ carboplatin was administered. Positively selected CD34+ cells were rapidly infused 24 hours after the end of high-dose chemotherapy. The time to neutrophil recovery greater than 0.5×10^(9)/L was 16 days and the time to platelet recovery greater than 50×10^(9)/L was 20 days.

인체 혈장에서 염산시프로플록사신(시프로플록사신으로서 250㎎) 정량을 위한 HPLC 분석법의 유효성검토

하용화,조성희,천성국,서성훈,류재환,최영욱,이경태 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.4

A simple HPLC method with ultraviolet detection of ciprofloxacin in human plasma was developed and validated. After protein precipitation with trichloroacetic acid, chromatographic separation of ciprofloxacin in plasma was achieved at 50℃ with a C_18 column and methanol-phosphate mixture (pH 2.5), as mobile phase. Quantitative determination was performed by ultraviolet detection at 278 nm. The method was specific and validated with a limit of quantification of 100 ng/ml. The infra- and inter-day coefficients of variation were between 1.67% and 10.55% and accuracy between 92.01% and 106.09%. The method has been successfully applied in a bioavailability study of 250 ㎎ ciprofloxacin hydrochloride tablet.

한약재가 Aspergillus parasiticus R-716의 生育과 aflatoxin 生成에 미치는 影響

구성회,이용욱,정덕화,김종규 한국식품위생학회 1988 식품위생학회지 Vol.3 No.2

한의원에서 오래 前부터 사용되어 오고 있는 몇 종의 한약재를 구입하여 이들을 chloroform으로 추출하여 추출물을 조제한 다음 공시균(Aspergillus parasiticus R-716)의 生育과 aflatoxin 生成에 미치는 影響을 조사한 결과 다음과 같은 結論을 얻었다. 1. 목단 추출물 첨가군에서 가장 현저하게 공시균의 生育이 저해되었고 그외의 첨가군에서도 강황, 봉출, 향부자, 백작액, 백출의 順으로 저해되었다. 2. Aflatoxin 生成은 백출과 강황 추출물 첨가군에서만 저해되었고, 나머지 첨가군에서는 오히려 증가되거나 효과가 적었다. 3. 백출 추출물 0.2ml첨가시 3일째부터 菌體 生成을 시작하여 9일째에 0.953g/30ml이었고, aflatoxin 함량은 792㎍으로서 대조군에 비해 약 50%가 감소되었다. 菌體 g當 aflatoxin 함량은 992㎍으로서 대조군의 1,467㎍에 비해 역시 크게 감소되었으며 NADPH oxidase 活性은 대조군보다 높아 백출 추출물이 공시균의 生育과 aflatoxin 生成에 저해 효과가 있는 것으로 나타났다. The possible effects of some oriental herbs, which have been used to treat cancerlike disease in Korea, on growth and aflatoxin production by Aspergillus parasiticus R-716 were investigated. Zedoaria rhizoma, Curcuma longa, Cyperus rotundus, Angelica gigas, Paeonia albiflora, Paeonia mountan, Atractylis ovata, and Pulsatilla koreana were extracted with chloroform. Among them the extract of Paeonia mountan was remarkably effective on the growth inhibition, and Curcuma longa, Zedoaria rhizoma, Cyperus rotundus, Paeonia albiflora, Atractylis ovata also inhibited the growth. The extract of Atractylis ovata and Curcuma longa, also inhibited the aflatoxin production but the others showed no effect at all or sometimes stimulated effect. With the addition of 0.2ml extract of Atractylis ovata in 30ml SLS medium, the growth was delayed for about 2 days, and after 9 days, mycelium weight was 0.953g, and total aflatoxin was reduced 50%(792㎍)of that produced in the control(1547㎍). Aflatoxin per mycelium weight was decreased 32%(992㎍)of that produced in the control(1467㎍), but NADPH oxidase was higher as compared to the control. The extract of Atractylis ovata appeared to have a inhibitory effect on the growth and aflatoxin production of Aspergillus parasiticus R-716.

골격성 Ⅲ급 부정교합자의 전치부 수직피개도에 영향을 미치는 요소 - 개교군과 피개군간의 비교

정정화,남형진,강상욱,손우성 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

12주간의 유산소성 운동이 정액 특성에 미치는 영향

박원화,김태욱,오상덕 한양대학교 체육과학연구소 2003 體育科學 Vol.23 No.23

The purpose of this study was to investigate the change of seminal characters by Aerobic Exercise. Subject divided into systemic trained by composed of treadmile running or walking by 70% of HRmax for 12 weeks in Aerobic Exercise group(n=5) and not participate in systematic training program in none training group(n=5) by the department of physical education in H University. The result were as follows: Seminal Characters 1)Concentration showed no significant differences, in aerobic exercise group and none training group decrease among training duration. 2)Motility showed significant differences in aerobic exercise group increase(p<.05) and no significant differences in none training group among training duration. 3)Repid Cell showed significant differences in aerobic exercise group increase(p<.05) and no significant differences in none training group among training duration. 4)VSL showed no significant differences in aerobic exercise traing group and none training group among training duration. 5)VCL showed no significant differences in aerobic exercise trains group and none training group among training duration. 6)STR showed no significant differences, in aerobic exercise trains group and none training group among training duration. 7)BCF showed no significant differences, in aerobic exercise trains group and none training group among training duration.

Azoloquinoxaline類의 간편한 合成에 관한 硏究 : 第1報

郭美玉,南順花,金恩慶,金眞姬,韓盛旭,李萬佶,朴義煥,金浩植 고신대학교 자연과학연구소 1993 고신대학교 자연과학연구소 논문집 Vol.3 No.-

The reaction of 2, 6-dichoroquinoxaline with m-chloroperbenzoic acid gave 2, 6-dichloroquinoxaline 4-oxide, whose reaction with sodium azide provided 7-chlorotetrazolo[1, 5-a]quinoxaline 5-oxide and not the isomeric azide. The structures of the synthesized compounds were confirmed on the basis of IR, ¹H-NMR, elemental analysis and mass spectral data.

유산소성 운동 강도가 혈중 알코올 농도에 미치는 영향

오승렬,오상덕,김태욱,박원화 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This study was to examine the most effective exercise intensity to decrease blood alcohol level under the condition of the same exercise volume after alcohol ingestion The subjects of the study were 7 students who enrolled in physical education major at H University, The blood alcohol levels of subjects were recorded and analyzed in different exercise condition, which were without exercise, HRmax 50% aerobic exercise, HRmax 70% aerobic exercise at each exercise situation(before, immediately after, 65rnins after, and 120mins after exercise). The effects of aerobic exercise on blood alcohol level and the changes in blood alcohol concentration level according to aerobic exercise intensity were as follows : 1. The blood alcohol levels in non-exercise group, HRmax 50% exercise group, and HRmax 70% exercise group illustrated significant difference(p<.05), In post hoc test, there was significant difference between non-exercise group and HRmax 50% exercise group (p<.05). In addition, there was significant difference between non-exercise group and HRmax 70% exercise group(p<.05). However, the blood alcohol levels in HRmax 50% exercise group and HRmax 70% exercise group did not show significant difference(p>.05). 2. The levels of decreasing blood alcohol concentration in the exercise situation(before, immediately after, 60mins after, and 120mins after exercise) showed significant difference(p<.001). 3. The interactive effect between exercise group and exercise situation illustrated significant difference(p<.001). As mentioned above, aerobic exercise was effective to decrease blood alcohol level after alcohol ingestion but under the condition of the same exercise volume the change of blood alcohol levels according to exercise intensity did not show significant difference. In conclusion, the most effective and fastest way to decrease blood alcohol level was HRmax 50% aerobic exercise, that is low intensity, rest after the exercise, and slight physical activity.

Streptozotocin 유도 당뇨쥐에서의 Phospholipase A₂, Cyclooxygenase 활성과 Thromboxane 및 Prostacyclin합성

양정아,김성옥,최정화,곽오계,이순재,장현욱 영남대학교 약품개발연구소 1998 영남대학교 약품개발연구소 연구업적집 Vol.8 No.-

당뇨병에서의 혈전생성 기전에 관련된 주된 요인을 관찰코져 흰쥐를 4주간 사육한 후 streptozotocin(STZ)으로 당뇨를 유발한 후 6일째에 희생하여 당뇨쥐에 있어서의 phospholipase A₂ 활성변화에 따른 조직의 과산화적 손상과 혈소판 응집능 등의 변화를 관찰한 결과는 다음과 같다. 체중증가는 STZ를 투여한 후에는 STZ 투여군에서 현저하게 감소하였다. 식이섭취에 있어 STZ injection 후, DM군은 16% 증가하였다. 식이효율은 STZ injection 후 DM군은 정상군에 비해 유의적으로 감소하였다. 혈청 중의 총 지질과산화물가는 정상군에 비해 DM군은 2.5배 높았다. HDL 중의 TBARS는 정상군에 비해 DM군은 약 1.6배 정도 높았고 LDL 중의 TBARS는 정상군에 비해 DM군에서 4.8배의 높은 증가를 보였다. 혈소판 phospholipase A₂ 활성은 DM군은 정상군에 비해 42% 증가하였다. 혈소판 cyclooxygenase 활성은 정상군에 비해 DM군이 2배 정도 높은 수준이었다. 혈소판 중의 TXA₂ 농도는 정상군과 비교하여 DM군에서 169%로 현저하게 높았고 대동백 PGI₂의 농도는 정상군에 비해 DM군은 낮은 수준을 보였으며 대동맥 PGI₂와 혈소판 TXA₂의 비는 DM군에서 정상군에 비해 55% 낮았다. 결론적으로 STZ 유발 당뇨쥐에 있어서는 혈청 지질과산화물 수준의 증가와 더불어 phospholipase A₂ 활성이 증가되었으며 따라서 cyclooxygenase가 유도하는 AA cascade 활성화에 의해 TXA₂ 생성증가 및 PGI₂/TXA₂ ratio의 감소가 관찰되었다. 이러한 결과는 당뇨병 질환에서 나타나는 동맥경화증, 심혈관계 질환 등의 여러 혈관계의 병리적 현상들이 당뇨 상태에서의 지질대사 이상으로 인한 지질과산화물의 증가와 그의 독성으로 인한 여러 혈소판 관련 인자들의 활성화와 밀접하게 연관되어 나타나는 것을 시사하는 것이라 하겠다. The relation between lipid peroxidation and thrombotic reaction were investigated in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats weighing 100±10gm were randomly assigned to normal and STZ-induced diabetic group(DM). Diabetes was experimentally induced by intravenous injection of 55mg/kg of body weight of STZ in citrate buffer(pH 4.3) after 4 weeks feeding of basal diet. Animals were sacrificed at the 6th day of diabetic states. Body weight gains were lower in diabetic group after STZ injection. Serum levels of thiobarbituric acid reacting substances(TBARS) that were markedly increased in DM group compared with of normal group. TBARS levles of HDL and LDL were similar patterns to total TBARS of serum. Activities of platelet phospholipase A₂(PLA₂) were higher in diabetic group than those of normal group. Activities of platelet cyclooxygenase were 106% in DM group than normal group. Platelet thromboxane A₂(TXA₂) formation was increased in DM group than normal group. Production of aortic prostacyclin(PGI₂) was lower in diabetic group than that of normal group. PGI₂/TXA₂ ratios were decreased by 55% in DM groups than those of normal group. The present results indicate that STZ-induced diabetic rats are more sensitive to oxidative stess which leads to acceleration of lipid peroxidation and platelet aggregability. In conclusion, accelerating effect of lipid peroxidation and thrombogenesis in diabetic state is regarded to be resulted from enhancement of PLA₂ activity and arachidonic acid metabolism, inhibition of antiaggregating agent and aortic PGI₂ formation.

DAF 공정에서 무기 고형입자의 유체역학적 충돌효율과 부상특성

곽동희,김성진,이화경,정흥조,이재욱,정팔진 대한상하수도학회 2002 상하수도학회지 Vol.16 No.6

Separation characteristics of inorganic particles occurred during heavy rainwater were investigated in DAF (dissolved air flotation) process. In order to remove the inorganic particles effectively, the collision and flotation efficiencies were examined from a hydrodynamic point of view. Generally, the collision efficiency increased with floc size under the variation of fluid dynamic conditions including inertial force. However, more precise model should be required to analysis the collision efficiency expressed both the physical properties for inorganic particles and hydrodynamic conditions for a reactor.

농업인 교육 프로그램 분석

김진모,김진군,마상진,전영욱,이진화,주현미 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.1

이 연구는 농업인을 대상으로 한 교육 프로그램의 내용 및 방법을 살펴봄으로써 향후 농업인 교육을 위한 기초자료를 제공하는 데 목적이 있었다. 농업인 교육프로그램 분석을 위한 개념적 틀은 관련문헌에 대한 고찰과 수집 자료에 대한 귀납적 분석을 통해 개발되었다. 그 결과, 8가지의 기관 유형, 17가지의 프로그램 내용 영역, 5가지의 교육방법, 3가지의 교육인원, 3가지의 교육기간이 도출되었다. 분석대상은 총 173개 기관의 2,483개 프로그램이었다. 이 연구의 주요 결과는 다음과 같다. 첫째, 우리나라의 농업인 교육은 국가기관 및 기술센터 등의 정부기관 주도로 이루어지고 있었다. 둘째, 재배/사육기술, 생활개선 및 농촌건강관리, 정보획득 등의 내용이 주요 교육내용으로 다루어지고 있었다. 셋째, 각 기관 유형별로 비중 있게 다루는 주요 교육내용에 있어서 다소 차이가 있었다. 넷째, 농업인 교육프로그램에서 활용되는 교육방법은 대체적으로 강의에 편중되어 있었다. 다섯째, 교육인원에 있어 중규모와 대규모 인원의 비중이 상대적으로 높았으며, 1 대 1 개별 교육은 전혀 없는 것으로 나타났다. 여섯째, 1일 혹은 3일 이상 이루어지는 교육의 비중이 상대적으로 높았으며, 2일 동안 이루어지는 교육의 비중은 비교적 낮은 편이었다. The purposes of this study were to analyze the farmer education programs in Korea, and to get implications for further program development. The framework for the analysis was developed through extensive literature review and an inductive method. As a result, eight types of organizations for farmer education, seventeen content domains of programs, five instructional methods, three categories of program participant size, and three categories of program period were derived. The subjects of analysis were 2,483 programs of 173 farmer education organizations. The major findings were as follows: 1) Farmer education programs of Korea were led by government agencies such as national organizations and extension agencies. 2) The content domains of cultivating/raising techniques, improvement of life quality and farmer health care, and information acquisition were relatively highly dealt with. 3) Main contents which were dealt with by each type of farmer education organization were differentiated. 4) In terms of instructional method, a lecture had the highest portion in most contents domains. 5) The portion of the middle size and large size of participants was relatively higher than others. And there are no programs which used one by one teaching method. 6) The portion of programs for 1 day or 3days was relatively higher, and the portion of programs for 2 days was lower.