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- 저자 : ( Qing Yu He ) (검색결과 39 건)
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He, Feng,Wen, Hai-Shen,Li, Ji-Fang,Yu, Da-Hui,Ma, Rui-Qin,Shi, Dan,Mu, Wei-Jie,Zhang, Yuan-Qing,Hu, Jian,Liu, Miao,Han, Wei-Guo,Zhang, Jia-Nan,Wang, Qing-Qing,Yuan, Yu-Ren,Liu, Qun Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.4
Gonadotropin-releasing hormone receptor (GnRHR) gene is expressed at the anterior pituitary gland and plays a key role in gonad development. This study aimed to investigate molecular genetic characteristics of the GnRHR gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of GnRHR gene on sex steroid level in Japanese flounder (Paralichthys olivaceus). We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the GnRHR gene in 75 individuals. We identified three SNPs in the GnRHR gene: P1 locus (C759A and C830T) in the coding region of exon2 which were both linked together and P2 locus (G984T) in the coding region of exon3, which added a new transcript factor (ADR1) and a new methylation site (CG). Only C830T of P1 leads to amino acid changes Thr266Ile. Statistical analysis showed that P1 was significantly associated with $17{\beta}$-estradiol ($E_2$) level (p<0.01) and gonadosomatic index (GSI) (p<0.05). Individuals with genotype BB of P1 had significantly higher serum $E_2$ levels (p<0.01) and GSI (p<0.05) than those of genotype AA or AB. Another SNP, P2, synonymous mutation, was significantly associated with GSI (p<0.05). Individuals with genotype AB of P2 had significantly higher GSI (p<0.05) than that of genotype AA. In addition, there was a significant association between one diplotype based on three SNPs and reproductive traits. The genetic effects for both serum $E_2$ level and GSI of diplotype D4 were super diplotypes (p<0.05). These results suggest that the SNPs in Japanese Flounder GnRHR are associated with $E_2$ level and GSI.
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Zhou Qing-Qing,Zhang Wei,Yu Yu-Sheng,Li Hong-Yan,Wei Liang,Li Xue-Song,He Zhen-Zhen,Zhang Hong 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.4
Objective: To compare the reproducibility and performance of quantitative metrics between ZOOMit and conventional intravoxel incoherent motion (IVIM) magnetic resonance imaging (MRI) in the diagnosis of early- and mid-stage Sjögren’s syndrome (SS). Materials and Methods: Twenty-two patients (mean age ± standard deviation, 52.0 ± 10.8 years; male:female, 2:20) with early- or mid-stage SS and 20 healthy controls (46.9 ± 14.6 years; male:female, 7:13) were prospectively enrolled in our study. ZOOMit IVIM and conventional IVIM MRI were performed simultaneously in all individuals using a 3T scanner. Quantitative IVIM parameters - including tissue diffusivity (D), pseudodiffusion coefficient (D*), and perfusion fraction (f) - inter- and intra-observer reproducibility in measuring these parameters, and their ability to distinguish patients with SS from healthy individuals were assessed and compared between ZOOMit IVIM and conventional IVIM methods, appropriately. MR gland nodular grade (MRG) was also examined. Results: Inter- and intra-observer reproducibility was better with ZOOMit imaging than with conventional IVIM imaging (ZOOMit vs. conventional, intraclass correlation coefficient of 0.897–0.941 vs. 0.667–0.782 for inter-observer reproducibility and 0.891–0.968 vs. 0.814–0.853 for intra-observer reproducibility). Significant differences in ZOOMit f, ZOOMit D*, conventional D*, and MRG between patients with SS and healthy individuals (all p < 0.05) were observed. ZOOMit D* outperformed conventional D* in diagnosing early- and mid-stage SS (area under receiver operating curve, 0.867 and 0.658, respectively; p = 0.002). The combination of ZOOMit D*, MRG, and ZOOMit f as a new diagnostic index for SS, increased diagnostic area under the curve to 0.961, which was higher than that of any single parameter (all p < 0.01). Conclusion: Considering its better reproducibility and performance, ZOOMit IVIM may be preferred over conventional IVIM MRI, and may subsequently improve the ability to diagnose early- and mid-stage SS.
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Yan-Qing Duan,Song-Tao He,Qing-Qing Li,Ming-Feng Wang,Wen-Yuan Wang,Wei Zhe,Yong-Hong Cao,Ming-He Mo,Yu-Long Zhai,Wen-Jun Li 한국미생물학회 2013 The journal of microbiology Vol.51 No.3
A Gram-positive, catalase- and oxidase-positive, strictly aerobic, endospore-forming rod bacterium, designated K3514T, was isolated from the leaves of Nicotiana tabacum. The strain was able to grow at temperatures of 8–40°C, pH 5.0–10.0 and NaCl concentrations of 0–7%. The predominant quinones (>30%) of this strain were MK-7(H2) and MK-7. Phylogenetic analysis of 16S rRNA gene sequence showed that strain K3514T was affiliated to the genus Lysinibacillus, with its closest relatives being Lysinibacillus mangiferihumi (98.3% sequence similarity), Lysinibacillus sphaericus (97.9% sequence similarity), Lysinibacillus fusiformis (97.4% sequence similarity), and Lysinibacillus xylanilyticus (97.3% sequence similarity). However, low levels of DNA-DNA relatedness values suggested that the isolate was distinct from the other closest Lysinibacillus species. Additionally, based on analysis of morphological, physiological, and biochemical characteristics, the isolate could be differentiated from the closest known relatives. Therefore, based on polyphasic taxonomic data, the novel isolate likely represents a novel species, for which the name Lysinibacillus tabacifolii sp. nov. and the type strain K3514T (=KCTC 33042T =CCTCC AB 2012050T) are proposed.
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Qing-Yong Wang,He-Rui Zhang,Yu Gao,Rong-Hai Li,Xiao-Hong Shang 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.5
Dear Editor, Persistent infection with human papillomavirus (HPV) is the main cause of cervical cancer. Screening of cervical specimens for high-risk human papillomaviruses (HR-HPV) has become a routine part of patient care in most countries. The Roche cobas 4800 system (Roche Molecular Systems Inc., Pleasanton, CA, USA), a fully automated system that uses in vitro qualitative real-time PCR technology, displays high sensitivity and specificity for HR-HPV detection [1]. However, a small proportion of invalid results may occur in specimens showing mucopurulent discharge because of pipetting errors, clot formation, or failed amplification, which could cause a delay in the results and lead to additional waiting time for patients and extra work hours for staff, although most cases are resolved by vortexing the specimens and retesting. To the best of our knowledge, no researcher has tried to overcome this problem by pretreating specimens with an appropriate concentration of Sputasol (Oxoid Ltd., Basingstoke, United Kingdom); Sputasol (dithiothreitol 1.4%) has been used to aid culturing and DNA extraction from sputum specimens in some laboratories [2]. Here, we propose a standard method to improve HR-HPV detection without affecting the performance of the cobas 4800 HPV system.
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Control Strategy for Buck DC/DC Converter Based on Two-dimensional Hybrid Cloud Model
Qing-Yu Wang,Ren-Xi Gong,Li-Wen Qin,Zhao-He Feng 대한전기학회 2016 Journal of Electrical Engineering & Technology Vol.11 No.6
In order to adapt the fast dynamic performances of Buck DC/DC converter, and reduce the influence on converter performance owing to uncertain factors such as the disturbances of parameters and load, a control strategy based on two-dimensional hybrid cloud model is proposed. Firstly, two cloud models corresponding to the specific control inputs are determined by maximum determination approach, respectively, and then a control rule decided by the two cloud models is selected by a rule selector, finally, according to the reasoning structure of the rule, the control increment is calculated out by a two-dimensional hybrid cloud decision module. Both the simulation and experiment results show that the strategy can dramatically improve the dynamic performances of the converter, and enhance the adaptive ability to resist the random disturbances, and its control effect is superior to that of the current-mode control.
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Yu, Qing,Liu, Shan-Ling,Wang, He,Shi, Gang,Yang, Pei,Chen, Xin-Lian Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11
In cervical cancer, one of the most common malignant tumors in women worldwide, miR-126 has been reported to exhibit decreased expression. However, its role in cervical cancer cell proliferation and drug sensitivity has remained relatively unexplored. Here, we compared the expression of miR-126 in cervical cancer tissues (n = 20) with that in normal cervical tissue (n = 20) using quantitative RT-PCR. The viability of Siha cervical cancer cells was further measured by MTT assay after transfection with miR-126 mimic (Siha-miR-126 mimic) or microRNA mimic negative control (Siha-miR mimic NC) and after treatment with various concentrations of bleomycin (BLM). IC50s were calculated, and the survival rates (SRs) of Siha cells were calculated. miR-126 expression in cervical cancer tissue was significantly decreased compared with that in normal cervical tissue (P < 0.01). The relative SRs of Siha-miR-126 mimic cells were also significantly decreased compared with those of Siha-miR mimic NC cells at 24-96 h after transfection. The IC50 of BLM in Siha-miR-126 mimic cells ($50.3{\pm}2.02{\mu}g/mL$) was decreased compared with that in Siha-miR mimic NC cells ($70.5{\pm}4.33{\mu}g/mL$) at 48 h after transfection (P < 0.05). Finally, the SRs of Siha-miR-126 mimic cells were significantly lower than those of SihamiR mimic NC cells after cultured in medium containing 40 ${\mu}g/mL$ BLM for 24-96 h (P < 0.05). These results suggest that miR-126 is expressed at low levels in cervical cancer. Upregulation of miR-126 inhibited cervical cancer cell proliferation and enhanced the sensitivity to BLM. Thus, miR-126 may represent a novel approach to cervical cancer treatment.
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Zhao-He Feng,Ren-Xi Gong,Qing-Yu Wang 대한전기학회 2014 Journal of Electrical Engineering & Technology Vol.9 No.4
The state transition matrix are obtained by solving state equations in terms of Laplace inverse transformation and Cayley-Hamilton theorem, and an establishment of a precise discreteiterative mapping of the voltage-fed buck-boost converter operating in discontinuous conduction mode is made. On the basis of the mapping, the converter bifurcation diagrams and Lyapunov exponent diagrams with the input voltage, the resistance, the inductance and the capacitance as the bifurcation parameters are obtained, and the effect of the parameters on the system stability is deeply studied. The results obtained show that they have a great influence on the stability of the system, and the general trend is that the increase of either the voltage-fed coefficient, input voltage or the load resistance, or the decrease of the filtering inductance, capacitance will make the system stability become poorer, and that all the parameters have a critical value, and when they are greater or less than the values, the system will go through stable 1T orbits, stable 2T orbits, 4T orbits, 8T orbits and eventually approaches chaos.
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Control Strategy for Buck DC/DC Converter Based on Two-dimensional Hybrid Cloud Model
Wang, Qing-Yu,Gong, Ren-Xi,Qin, Li-Wen,Feng, Zhao-He The Korean Institute of Electrical Engineers 2016 Journal of Electrical Engineering & Technology Vol.11 No.6
In order to adapt the fast dynamic performances of Buck DC/DC converter, and reduce the influence on converter performance owing to uncertain factors such as the disturbances of parameters and load, a control strategy based on two-dimensional hybrid cloud model is proposed. Firstly, two cloud models corresponding to the specific control inputs are determined by maximum determination approach, respectively, and then a control rule decided by the two cloud models is selected by a rule selector, finally, according to the reasoning structure of the rule, the control increment is calculated out by a two-dimensional hybrid cloud decision module. Both the simulation and experiment results show that the strategy can dramatically improve the dynamic performances of the converter, and enhance the adaptive ability to resist the random disturbances, and its control effect is superior to that of the current-mode control.
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