Effects of HA and NA glycosylation pattern changes on the transmission of avian influenza A(H7N9) virus in guinea pigs

Park, S.,Lee, I.,Kim, J.I.,Bae, J.Y.,Yoo, K.,Kim, J.,Nam, M.,Park, M.,Yun, S.H.,Cho, W.I.,Kim, Y.S.,Ko, Y.Y.,Park, M.S. Academic Press 2016 Biochemical and biophysical research communication Vol.479 No.2

Avian influenza H7N9 virus has posed a concern of potential human-to-human transmission by resulting in seasonal virus-like human infection cases. To address the issue of sustained human infection with the H7N9 virus, here we investigated the effects of hemagglutinin (HA) and neuraminidase (NA) N-linked glycosylation (NLG) patterns on influenza virus transmission in a guinea pig model. Based on the NLG signatures identified in the HA and NA genetic sequences of H7N9 viruses, we generated NLG mutant viruses using either HA or NA gene of a H7N9 virus, A/Anhui/0½013, by reverse genetics on the 2009 pandemic H1N1 virus backbone. For the H7 HA NLG mutant viruses, NLG pattern changes appeared to reduce viral transmissibility in guinea pigs. Intriguingly, however, the NLG changes in the N9 NA protein, such as a removal from residue 42 or 66 or an addition at residue 266, increased transmissibility of the mutant viruses by more than 33%, 50%, and 16%, respectively, compared with a parental N9 virus. Given the effects of HA-NA NLG changes with regard to viral transmission, we then generated the HA-NA NLG mutant viruses harboring the H7 HA of double NLG addition and the N9 NA of various NLG patterns. As seen in the HA NLG mutants above, the double NLG-added H7 HA decreased viral transmissibility. However, when the NA NLG changes occurred by a removal of residue 66 and an addition at 266 were additionally accompanied, the HA-NA NLG mutant virus recovered the transmissibility of its parental virus. These demonstrate the effects of specific HA-NA NLG changes on the H7N9 virus transmission by highlighting the importance of a HA-NA functional balance.

비선형 연립방정식의 해를 이용한 캡슐형 무선 내시경 모듈의 위치 추적 방법

남혜원,이종학,이정현,박희준,원철호,조진호 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

임신중 난소종양의 임상적 병리학적 고찰

조태호,이권해,남계현,이정재,이해혁 순천향대학교 1990 논문집 Vol.13 No.1

순천향대학병원 산부인과에서 1974년부터 1988년까지 경험한 임신중 난소종양 54예에 대한 임상적병리학적 고찰을 하여 다음과 같은 결과를 얻었다. 1. 임신중 난소종양의 빈도는 같은 기간의 총분만수에 대해 1 : 391.9였고 전부 양성종양이었다. 2. 임신중 난소종양의 연령분포는 20∼42세였고 평균연령은 26.9세였다. 3. 출산력은 평균 분만횟수가 0.99회이고 75.9%에서 0∼1회의 분만횟수를 갖고 있었다. 4. 난소종양의 초진시 주소, 증상및 증후로는 하복통이 19예(35.2%)로 제일 많았으며, 질점상출혈과 성기출혈이 4예(7.4%), 종양촉진이 14예(25.9%)등이었다. 증상및 증후가 없었던 17예(31.5%)는 산전관리시 진단되었다. 5. 종양의 진단시기는 임신초기에 31예(57.4%), 임신중기에 16예(29.6%), 말기에 7예(13%)이었다. 6. 난소종양의 병리조직학적 소견에서 양성기형종이 19예(35.2%)로 제일 많았으며, 장액성낭선종이 6예(11.1%), 점액성낭선종과 단순낭종이 각 4예(7.4%)였으며 병리학적 결과를 알 수 없는 경우도 4예가 없었다. 7. 종양의 크기를 보면 직경 6cm미만이 17예 6∼10.9cm가 30예로 대부분을 차지하였으며 11∼14.9cm이 7예였다. 8. 임신초기에 합병증으로 수술한 12예중 9예는 염전으로 인해 수술을 시행하였다. 9. 난소종양의 발생부위는 좌측이 많아 31예(57.4%), 우측이 18예(33.3%)였으며 5예(9.3%에서 양측성이었다. 10. 수술방법은 보존적 낭종절제술, 난소절제술, 난소난관절제술등을 시행하였으며, 보조적 요법으로는 안정및 황체호르몬, 아편팅크, 완화제 등을 투여하였다. 11. 임신의 경과를 보면 만기분만 17예중 정상분만이 7예 제왕절개가 10예였고 수술후 자연 유산된 경우는 3예였다. Clinical and pathologic review of ovarian tumors in pregnancy was presented. During the 15 years from 1974 to 1988 there were 54 patients at the Department of Obstetrics and Gynecology, Soonchunhyang University Hospital. The result obtained follows: 1) The incidence of tumors in pregancy was 1 in 391.9 pregnancies and they were all benign. 2) Mean age was 26.9 years old and the range was 20∼42 years old. 3) Mean parity was 0.99. 4) As to the initial chief complaints or the mode of presentation, low abdominal pain was found in 19 cases(35.2%), vaginal spotting or bleeding in 4 cases(7.4%), palpable mass in 14 cases(25.9%). In the 17 cases(31.5%), free of symptoms or signs were detacted at antenatal care. 5) 31 cases(57.4%) were operated in first trimester, 16 cases(29.6%) in the second trimester, 7 cases(13%) in the third trimester. 6) Histopathology of those cases were benign cystic teratoma in 19 cases(35.2%), serous cystadenoma in 6 cases(11.1%), simple cyst in 4 cases(7.4%), mucinous cystadenoma in 4 cases(7.4%). 7) Size of tumor was under 6cm in 17 cases, 6∼10.9cm in 30 cases, 11∼14.9cm in 7 cases. 8) Complications of tumor were occurred in 12 cases, being 9 cases of torsion, 3 cases of rupture. 9) The left side tumor was more common being 31 cases(57.4%), and the right was 18 cases(33.3%) and 5 cases(9.3%) of tumor were bilateral. 10) Treatment was surgical being conservative cystectomy, salpingoophorectomy, adjuvant treatment were bed rest and administration of opium tincture, petrolatum and progesterone injection during the hospital day. 11) Outcome of pregnancy and mode of delivery; Spontaneous abortion occurred in 3 cases, full term delivery was noted in 17 cases, and Cesarean section in 10 cases.

우리나라 1개 농촌지역사회 주민의 질병실태에 관한 조사

김경한,남형자,송혜섭,조현실 이화여자대학교 의과대학 1975 梨花醫學誌 Vol.- No.7

A morbidity survey was carried out in order to collect the health data of people in a Korean rural community, Su-dong Myun, Yang-ju Gun, from August 12 to 16, 1974 through interview with 207 patients, and the following results were obtained: 1. Prevalance rate was 150 per 1000 for male, 157 for female, and average prevalance rate was 150 per every 1000. 2. 55∼59 age group was 263 as the highest, and 10∼14 age group was 100 as the lowest per every 1000. 3. Following was th order of five major diseases which were classified with I.C.D.: 1) Disease of the Digestive System (ⅠⅩ) 43.0% 2) Disease of the Skin and Subcutaneous Tissue (ⅩⅡ) 12.6% 3) Disease of the Musculoskeletal System (ⅩⅢ) 12.6% 4) Disease of the Respiratory System (Ⅷ) 11.1% 5) Accidents, Poisoning and Violence (ⅩⅦ) 7.7% 4. Following was the order of three major disease for 98 patients in short term illness which were classified with I.C.D.: 1) Disease of the Digestive System (Ⅸ) 51.0% 2) Disease of the Skin and Subcutaneous Tissue (ⅩⅡ) 19.8% 3) Accidents, Poisoning and Violence (ⅩⅦ) 12.2% 5. For short term illness, the recovery times were 3 days in 40.8% and 7 days in 72.4%. 6. In 265 cases, the treated were 146. 7. Uses of medical facilities were Su-Dong Myun 48.3%, Hwa-Do Myun 27.6%, Seoul 12.4%, etc, 12.4%, respectively. 8. The average medical cost was 6458 Won in Herb drug store, 3240 won in Hospital, 967 won in drug store.

임신중 난소종양의 임상적 병리학적 고찰

이해혁,이정재,남계현,이권해,조태호 순천향대학교 1990 Journal of Soonchunhyang Medical Science Vol.13 No.1

우리나라 1개 농촌지역사회 주민의 질병실태에 관한 조사

김경한,남형자,송혜섭,조현실 梨花女子大學校 醫科大學 1974 梨花醫學誌 Vol.- No.7

Investigation on Antibacterial and Antioxidant Activities, Phenolic and Flavonoid Contents of Some Thai Edible Plants as an Alternative for Antibiotics

Lee, J.H.,Cho, S.,Paik, H.D.,Choi, C.W.,Nam, K.T.,Hwang, S.G.,Kim, Soo-Ki Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.10

This study was aimed to examine the antibacterial and antioxidative properties of seven edible plants from Thailand to develop alternative antibiotics as feed additives. The plants include Citrus aurantifolia Swingle (Lime) fruits and its leaves, Sesbania grandiflora L. (Agati sesbania) leaves, Piper sarmentosum Roxb (Wild betal) leaves, Curcuma domestica Valeton (Turmeric) roots, Morinda citrifolia L. (Beach mulberry) leaves, Cassia siamea britt (Siamea cassia) leaves, and Cocos nucifera L. (Coconut) peels. The plants were extracted by methanol, n-hexane, chloroform, ethyl acetate, butanol and water. Antibacterial activities with minimum inhibitory concentration (MIC) were determined by agar diffusion assay against Escherichia coli, Burkholderia sp., Haemopilus somnus, Haemopilus parasuis, and Clostridium perfringens that were considered pathogenic strains in livestock infection. Methanol extracts of C. aurantifolia Swingle fruits and leaves showed the broadest spectrum of antibacterial activities except for C. perfringens. Butanol extract of S. grandiflora L. leaves showed the strongest activity against Burkholderia sp. with MIC, $135{\mu}g/mL$. P. sarmentosum Roxb leaves showed antibacterial activities against E. coli, Burkholderia sp. and H. parasuis. Ethyl acetate and water extracts from C. domesitca Valeton roots showed MIC of $306{\mu}g/mL$ and $183{\mu}g/mL$, respectively against only C. perfringens. Antioxidative activity was determined by 2-diphenyl-2-picryl hydrazyl photometric assay. The methanol extracts of C. aurantifolia Swingle fruits and P. sarmentosum Roxb leaves showed the highest antioxidant activity among all the extracts with 3.46 mg/mL and 2.70 mg/mL effective concentration 50% ($EC_{50}$) values, respectively. Total contents of phenolics and flavonoids were measured from the plant extracts. Methanol extracts of S. grandiflora L. and chloroform extracts of C. domestica Valeton were found to have the highest amount of total phenolics, 41.7 and $47.8{\mu}g/mL$, respectively. Flavonoid content of methanol extracts in S. grandiflora L. T was $22.5{\mu}g/mL$ and the highest among plant extracts tested. These results indicated that C. aurantifolia Swingle, S. grandiflora L., P. sarmentosum Roxb, and C. domestica Valeton have antibacterial and antioxidant activities and can be used as alternative antibiotics or potential feed additives for the control of animal pathogenic bacteria.

수소화 처리된 InAs/GaAs 양자점 적외선 수광소자의 전기적 특성

남형도,송진동,최원준,조운조,이정일,최정우,양해석,Nam H.D.,Song J.D.,Choi W.J.,Cho W.J.,Lee J.I.,Choe J.W.,Yang H.S. 한국진공학회 2006 Applied Science and Convergence Technology Vol.15 No.2

InGaAs/GaAs 양자 우물 내에 삽입된 InAs 양자점으로 구성된 5층의 흡수층과 $Al_{0.3}Ga_{0.7}As/GaAs$ SL (superlattice) 암전류 장벽층을 갖는 QDIP (quantum dot infrared photodetector) 구조에 대한 수소 RF 플라즈마에 의한 수소화 처리가 QDIP의 전기적. 광학적 특성에 미치는 영향에 대해 연구하였다. RF 플라즈마 수소화 처리는 양자점의 밴드구조에 영향을 미치지 않았으며 $Al_{0.3}Ga_{0.7}As/GaAs$ SL 암전류 장벽층 내의 결함 제거 및 QDIP 구조 내 결함 생성을 동시에 유도함으로써 QDIP의 전기적 특성 향상은 수소 플라즈마 처리시간의 함수임을 알았다. 20 W의 수소 RF 플라즈마를 사용했을 때, 10분간의 플라즈마 조사가 가장 좋은 전기적 특성을 제공하여 높은 암전류 때문에 원시료에서는 측정 할 수 없었던 광전류 신호를 측정 할 수 있었다. In this paper, we investigated the effect of hydrogen-plasma (H-plasma) treatment on the electrical and optical properties of a quantum dot infrared photodetector (QDIP) with a 5-stacked InAs dots in an InGaAs/GaAs well structure and $Al_{0.3}Ga_{0.7}As/GaAs$ SL (superlattice) current blocking layer. It has been observed that H-plasma treatment didn't affect the band structure of QDIP. It has been also observed that the H-plasma treatment on the QDIP not only enhance the electrical property of QDIP by curing the defect channels in $Al_{0.3}Ga_{0.7}As/GaAs$ SL but also introduce defects in QDIP structure. The H-plasma treatment for 10 min with 20 W of RF power provided the lowest dark current, which made it possible to measure the photo-current (PC) of QDIP whose PC was not detectable without the H-plasma treatment due to the high dark current.

Comparison of CpG island hypermethylation and repetitive DNA hypomethylation in premalignant stages of gastric cancer, stratified for Helicobacter pylori infection

Park, Seog-Yun,Yoo, Eun J,Cho, Nam-Yun,Kim, Nayoung,Kang, Gyeong H John Wiley Sons, Ltd. 2009 The Journal of pathology Vol.219 No.4

<P>CpG island hypermethylation and genomic DNA hypomethylation are found not only in gastric cancers but also in associated premalignant lesions. Helicobacter pylori infection induces aberrant CpG island hypermethylation in gastric mucosae. However, little is known about the relationship between H. pylori infection and aberrant methylation in premalignant lesions. The present study characterized methylation changes in a subset of genes and repetitive DNA elements (ALU, LINE-1, SAT2) and examined their relationship with H. pylori infection in premalignant lesions of gastric cancers. We performed MethyLight analysis of 25 genes and SAT2 and COBRA analysis of ALU and LINE-1 in 212 gastric tissue samples. H. pylori infection was closely associated with enhanced hypermethylation of CpG island loci in chronic gastritis samples, but this association was not found among intestinal metaplasias, gastric adenomas and gastric cancers. The number of methylated genes was greater in intestinal metaplasia and gastric adenoma samples than in chronic gastritis samples, regardless of H. pylori infection. Methylation of repetitive DNA elements in gastric lesions generally decreased with progression of the gastric lesion along the multistep carcinogenesis. No difference was noted in the number of methylated genes in chronic gastritis or intestinal metaplasia between gastric cancer patients and non-cancer subjects. In conclusion, we found that there was no enhanced CpG island hypermethylation in gastric cancer and premalignant lesions in association with H. pylori infection and our findings suggest that CpG island hypermethylation and repetitive DNA hypomethylation are enhanced with progression of the gastric lesion through the multistep carcinogenesis, regardless of the status of H. pylori infection. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</P>

소셜 데이팅 서비스 어플 사용에 따른 대학생의 성 지식, 성 태도와 성적 충동성에 관한 연구

김경미,김선영,김지은,김하영,김혜원,남소희,박수빈,옹희선,정한솔,조은주,박효정,이인영 이화여자대학교 간호학회 2018 이화간호학회지 Vol.- No.52

Purpose: The purpose of this study was to compare college students' sexual knowledge, sexual attitude, and sexual impulsivity according to their use of social dating service application. Method: The subjects were 197 college students. Data were analyzed using frequency, percentage, average, standard deviation, t-test, ANOVA, Chi-square test, Scheffe test and Pearson correlation using the SPSS program. Results: Among 197 subjects, 26.9% of the participants had answered to use the application. As the frequency of using applications increases, the level of sexual knowledge is low, sexual attitude is open, and sexual impulsivity is high. Significant association was observed between sexual knowledge, sexual attitude and sexual impulsivity. Conclusions: It was revealed that the use of social dating service application is highly relevant to the factors used in the study. Therefore the sex education should include new contents related to the social dating service application. Further research is needed on the other factors that influence the use of a social dating service application.