지속적 및 간헐적 가압력이 MC3T3-E1세포의 Alkaline phosphatase 활성도에 미치는 영향

송혜섭 慶北大學校 齒科大學 1994 慶北齒大論文集 Vol.11 No.2

지속적 및 간헐적 가압력이 조골양세포의 활성도에 미치는 차이를 알아보기 위하여, MC3T3-E1세포를 24well 배양접시에 배양한 후 밀생상태가 되었을 때, incubator 속의 특수 제작된 사각상자에 배양 접시를 넣고 타이머에 연결된 Diaphram pump를 이용하여 300g/㎠의 압력을 10분간 가압후 10분간 가압이 중지되도록 한 간헐적 가압군과 지속적으로 가한 가압군으로 하여 각각 실험 24시간, 48시간, 72시간후의 alkaline phosphatase활성도를 대조군과 비교하여 다음과 같은 결과를 얻었다. . 가압 24시간 후에서는 각 군간에 alkaline phosphatase 활성도에 유의한 차이가 나타나지 않았다. . 가압 48시간 후에서는 간헐적 가압군과 지속적 가압군 모두 대조군 보다 alkaline phosphatase 활성도가 증가 되었다. . 가압 72시간 후에서는 간헐적 가압군의 alkaline phosphatase 활성도 증가가 대조군에 비하여 더욱 현저하였으나 지속적 가압군에는 대조군에 비하여 유의한 차이가 나타나지 않았다. . 가압 72시간 후에서는 간헐적인 가압군이 지속적인 가압군에 비하여 alkaline phosphatase의 활성도가 증가되었다. . 가압에 의한 세포의 뚜렷한 형태학적인 변화는 관찰되지 않았다. The propose of this study was to evaluate the difference of cellular activity dependent on intermittent compressive force by determining the alkaline phosphatase activity. Alkaline phosphatase activity was measured on control and exprerimental groups every 24, 48, 72 hours. Experimental groups consisted of continous and intermittent compressive group which were compressed by 300g/㎠ of diaphram pump. The intermittent compressive group was connected by timer which was worked on 10 minutes an off 10 minutes. Th results were as follows; . The alkaline phosphatase activity between control and experimental groups showed not significant difference at compressed 24 hours. . The alkaline phosphatase activity of experimental groups were more increased than control group at compressed 48 hours. . The alkaline phosphatase activity of intermittent compressive group showed significant increased to control group. Whereby continuous compressive group showed not significant difference to control at 72 hours. . The alkaline phosphatase activity of intermittent compresssive group were stringly increased than continuous compressive groups. . Between experimental groups and control group no other morphologic changes were detected by microscopic findings.

지속적 및 간헐적 가압력이 MC3T3-E1세포의 Alkaline phosphatase 활성도에 미치는 영향

송혜섭,경희문 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.4

교정력에 의한 치아이동은 세포반응의 결과로 나타난다. 이에 힘의 양상에 따른 세포의 반응을 알아보고자 MC3T3-E1세포를 24well 배양접시에 배양한 후 밀생상태가 되었을 때, incubator 속의 특수 제작된 사각상자에 배양 접시를 넣고 타이머에 연결된 Diaphram pump를 이용하여 300 gm/㎠의 압력을 10분간 가압후 10분간 가압이 중지되도록 한 간헐적 가압군과 지속적으로 가한 가압군으로 하여 각각 실험 24시간, 28시간, 72시간후의 alkaline phosphatase 활성도를 대조군과 비교하여 다음과 같은 결과를 얻었다. 1. 가압 24시간 후에서는 각 군간에 alkaline phosphatase 활성도에 유의한 차이가 나타나지 않았다. 2. 가압 48시간 후에서는 간헐적 가압군과 지속적 가압군 모두 대조군 보다 alkaline phosphatase 활성도가 증가 되었다. 3. 가압 72시간 후에서는 간헐적 가압군의 alkaline phosphatase 활성도 증가가 대조군에 비하여 더욱 현저하였으나 지속적 가압군에는 대조군에 비하여 유의한 차이가 나타나지 않았다. 4. 가압 72시간 후에서는 간헐적인 가압군이 지속적인 가압군에 비하여 alkaline phosphatase의 활성도가 증가되었다. 5. 가압에 의한 세포의 뚜렷한 형태학적인 변화는 관찰되지 않았다. The propose of this study was to evaluate the difference of cellular activity dependent on intermittent compressive force by determining the alkaline phosphatase activity. Alkaline phosphatase activity was measured on control and experimental groups every 24, 48, 72hours. Experimental groups consisted of continous and intermittent compressive group which were compressed by 300g/㎠ of diaphram pump. The intermittent compressive group was connected by timer which was worked on 10 minutes an off 10minutes. The results were as follows ; 1. The alkaline phosphatase activity between control and experimental groups showed not significant difference at compressed 24 hours. 2. The alkaline phosphatase activity of experimental groups were more increased than control group at compressed 48 hours. 3. The alkaline phosphatase activity of intermittent compressive group showed significant increased to control group. Whereby continuous compressive group showed not significant difference to control at 72 hours. 4. The alkaline phosphatase activity of intermittent compressive group were stringly increased than continuous compressive groups. 5. Between experimental groups and control group no other morphologic changes were detected by microscopic findings.

Rh 음성 산모의 문제점

송혜섭(HS Song),장애숙(AS Chang),김정혜(JH Kim),양석기(SK Yang),민보은(BE Min) 대한산부인과학회 1982 Obstetrics & Gynecology Science Vol.25 No.7

1976년 1월 1일부터 1980년 7월 31일까지 일신부인병원 산부인과에서 분만한 Rh 음성 산모 43명과 53회의 임신으로 출생된 55명의 신생아를 대상으로 Rh 음성이 산모와 태아에 미치는 영향을 비교 관찰한 결과 다음과 같은 결과를 얻었다. 1.28,249명의 산모중 Rh 음성은 43명이었고 53회의 임신으로 그 빈도는 0.19%였다. 2.Rh 면역된 임신 5예에서 그 원인이 수혈에 의한 것으로 생각되는 것이 3예로 가장 많았고 이전의 인공유산에 의한 것으로 생각되는 것이 2예였다. 3.산후 Rhogam을 투여했던 9예에서는 모두 Rh면역이 일어나지 않았다. 4.Rh 음성 산모의 산전관리에서 Rh 항체역가치에만 의존하지 말고 양수천자에 의한 빌리루빈치의 측정이 필요한 것으로 생각되었다. 5.Rh 음성이 산모에 미치는 영향으로써 산후출혈 후 혈액공급이 어려운 것이었고, 분만전에 가족의 광범위한 혈액형 검사로 Rh 음성 혈액의 준비가 필요한 것으로 사료되었다. 6.Rh 면역된 신생아에서 주산기 사망은 없었으나 빠른 빌리루빈치의 증가가 나타남으로 제대혈액으로 혈액형, 혈색소치, 빌리루빈치, 직접쿰수치의 측정이 필요하고 출생 후 면밀한 관찰과 적극적인 처치가 요구된다. 53 pregnancies in 45 Rh neg. women who were delivered between Jan. 1st 1976 and July 30th 1980 in Il Sin Women`s Hospital were reviewed and following results were obtained. 1.The incidence of Rh neg. women among a total 28,249 patients was 0.19%. 2.5 patients were immunized. In 3 patients the immunization was probably due to previous transfusion of Rh positive blood and in 2 cases the immunization followed previously induced abortions. 3.The use of Rhogam after delivery, abortion, ectopic or possible placental trauma in the non-immunized patient prevents immunization and reduces the neonatal problem.In this series 9 patients who had been given Rhogam after previous delivery Rh immunization was not present. 4.In the management of pregnancy in a Rh negative women not only the Rh antibody titer but also amniocentesis with bilirubin absorption curve is required. 5.Danger to the mother was difficulty in obtaining Rh negative blood for transfusion after severe P.P.H. It is important to check blood grouping of many of the relatives as possible in order to have rapid collection of Rh negative blood when necessary. 6.There were no stillbirths or neonatal deaths, but 4 babies required exchange transfusion due to rapid increase of serum bilirubin. To lower the perinatal morbidity the cord blood needs to be examined in detail followed by close observation with active treatment if jaundice develops to a certain level.

근육세뇨관 수분 및 이온 이동에 대한 모델

양훈모,송혜섭,민영기 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

본 연구는 세뇨관 벽을 통하여 물과 이온이 이동하는 현상을 수학적 모델에 근거하여 수행하였다. 세포측막, tight junction, 기저막으로 구성된 측간질 공간으로의 염분이동은 삼투현상에의해 동시에 수분이동을 유발한다. 이로인한 수분에 공간내 증가는 정수합을 증가시켜 Na, Cl, HCO3 에 대한 반사계수가 0 이고 투과성이 매우 높은 기저막을 통해 용액이 유출된다. 동반이동 가능성과 비평형 열역학 이론을 포함한 본 모델을 이용하여 tight junction의 이온투과성과 반사계수가 물과 이온 이동에 미치는 영향을 조사해 본 결과는 다음과 같다. 1. 수분의 전도성(Lp)은 100배 이상하여야 삼투압에 의한 수분이동이 전체 수분이동에 영향을 미칠 수 있다. 2. 관강 측과 기저측 세포막은 반사계수가 1이기 때문에 세포를 관통하는 convective ion flux에는 영향이 없었다. 3. 관강내 액과 측공간 액사이에는 높은 HCO3 농도 경사가 유지되고 있기 때문에 HCO3에 대한 높은 반사계수는 삼투적 수분 흡수에 필수적이다. 4. Cl의 반사계수의 감소는 수분흡수를 증가시켜 Cl 재흡수도 증가시켰다. 동시에 전기적 확산에 의해 Na 재흡수도 증가하였다. 5. 전반적인 반사계수의 감소는 수분 재흡수는 감소 시켰으나 용질 재흡수는 증가시켰다.

Prenatal diagnosis of DMD by multiplex PCR with chorionic villi

이교원,송혜섭,최익준,최재호,이숙환,김성도,한종설 대한산부인과학회 1998 대한산부인과학회 학술대회 Vol.82 No.-

만삭분만의 예측지표로서의 태아 Fibronectin과 유도분만시 Misoprostol의 효용성 연구

김성도,한종설,송혜섭,최재호,이교원,김범영,신봉식,고염규 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.9

결론적으로 태아 fibronectin 정성 검사와 Bishop 점수를 병행하여 유도분만의 시기를 결정하는데 사용한다면 제왕절개 수술률을 감소시키면서 유도분만을 성공적으로 수행할 수 있을 것으로 사료된다. Objective: To evaluate the efficacy of misoprostol induced induction of term labor according to the presence fo fetal fibronectin in cervicovaginal secretion. Methods: 90 women scheduled for induction of labor were examined for Bishop score and were undergone for intravaginal application of 50 ㎍ of misoprostol at 6 hours interval. Misoprostols were administered continuously unless any of regular labor, spontaneous rupture of membrane or fetal distress occurred. In our study, 49 cases showed fibronectin positive cervicovaginal secretion, 41 cases showed fibronectin negative cervicovaginal secretion, 15 cases undergone for cesarean section. Results: 1. No significant difference were observed in Bishop`s score between the two groups. (4.72±0.98 versus 4.47±1.05). 2. Mean numbers of misoprostol administration were 1.33±0.57 and 2.03±1.03 in positive group and negative group respectively. Mean times required from labor induction to vaginal delivery were 470±388 min and 861±513 min respectively. Vaginal delivery in 12 hours were 79% and 50% in positive group and negative group respectively. 3. Dividing the cases according to the high and low Bishop score with cutoff level Bishop score of 5, mean numbers of misoprostol administration were 1.11±0.32 and 2.86±0.86 in high score group and low score group respectively. Mean times required from labor induction to vaginal delivery were 380±227 min and 1266±443 min in high score group and low score group respectively. Vaginal delivery in 12 hours were 89.9% and 14.3% in high score group and low score group respectively. Conclusion: Above study results suggest that fetal fibronectin qualitative immunoassay along with the evaluation of Bishop score might play important role in determining the appropriate timing of labor induction.

조기진통진단에 있어 혈중 부신피질 자극호르몬 방출인자의 유용성에 관한 연구

김성도,한종설,송혜섭,최병익,이교원,조필제,김범영,최익준 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.9

Human placenta synthesizes bioactive corticotropin releasing factor(CRF), a 41 amino acid peptide, which displays identical immunological, biological, and chemical characteristics to hypoth- alamic CRF. Placental CRF enters the maternal circulation and stimulates release of local placental prostaglandin E2 and F2α that have a central role in the mechanisms controlling uterine con- tractility and cervical softening. A large number of clinical investigators has suggested that place- ntal CRF may be involved in mechanisms leading to labor. As well as CRF`s rises in term preg- nancy, maternal serum CRF levels are also elevated in pregnancies complicated by preterm labor. So the clinical use of maternal serum CRF levels as a predictor of preterm labor was proposed. Therefore, we have carried out a prospective study on the efficacy of maternal serum CRF levels in diagnosis of preterm labor. In this study, the subjects consisted of 30 women admitted for the treatment of preterm labor and another 30 women with normal pregnancies between 28 and 36 completed weeks of gestation, and their serum CRF levels were assessed. The results were as follows: 1. In both groups, serum CRF levels were increased as being associated to gestational ages, especially in late pregnancy. 2. In a comparative study between two groups, the serum CRF values of pregnancies complicated by preterm labor were significantly higher than their values of control group(p$lt;0.05). 3. In pregnancies complicated by preterm labor, the serum CRF values of preterm delivery were not significantly elevated comparing with their values of other pregnancies complicated bypreterm labor(p$gt;0.05). 4. In pregnancies complicated by preterm labor, the serum CRF values of infection group were significantly lower than their values of non-infection group(p$lt;0.05).

우리나라 1개 농촌지역사회 주민의 질병실태에 관한 조사

김경한,남형자,송혜섭,조현실 梨花女子大學校 醫科大學 1974 梨花醫學誌 Vol.- No.7

우리나라 1개 농촌지역사회 주민의 질병실태에 관한 조사

김경한,남형자,송혜섭,조현실 이화여자대학교 의과대학 1975 梨花醫學誌 Vol.- No.7

A morbidity survey was carried out in order to collect the health data of people in a Korean rural community, Su-dong Myun, Yang-ju Gun, from August 12 to 16, 1974 through interview with 207 patients, and the following results were obtained: 1. Prevalance rate was 150 per 1000 for male, 157 for female, and average prevalance rate was 150 per every 1000. 2. 55∼59 age group was 263 as the highest, and 10∼14 age group was 100 as the lowest per every 1000. 3. Following was th order of five major diseases which were classified with I.C.D.: 1) Disease of the Digestive System (ⅠⅩ) 43.0% 2) Disease of the Skin and Subcutaneous Tissue (ⅩⅡ) 12.6% 3) Disease of the Musculoskeletal System (ⅩⅢ) 12.6% 4) Disease of the Respiratory System (Ⅷ) 11.1% 5) Accidents, Poisoning and Violence (ⅩⅦ) 7.7% 4. Following was the order of three major disease for 98 patients in short term illness which were classified with I.C.D.: 1) Disease of the Digestive System (Ⅸ) 51.0% 2) Disease of the Skin and Subcutaneous Tissue (ⅩⅡ) 19.8% 3) Accidents, Poisoning and Violence (ⅩⅦ) 12.2% 5. For short term illness, the recovery times were 3 days in 40.8% and 7 days in 72.4%. 6. In 265 cases, the treated were 146. 7. Uses of medical facilities were Su-Dong Myun 48.3%, Hwa-Do Myun 27.6%, Seoul 12.4%, etc, 12.4%, respectively. 8. The average medical cost was 6458 Won in Herb drug store, 3240 won in Hospital, 967 won in drug store.

Methotrexate로 치료 실패한 자궁경부 임신의 보존적 수술 치료 1 예

김성도,한종설,송혜섭,이교원,이상준,최익준 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.4

자궁경부 임신은 생명을 위협하는 자궁외 임신의 드문 종류로써 수정란이 자궁경관내구 이하의 경부 점막에 착상한 것이다. 대부분의 환자들이 낮은 출산률을 갖고 있으므로 최근 치료의 동향은 그들의 임신 기능을 보존하는 것이다. 보존적 치료시에 가장 주의해야 할 것은 임신산물 제거후의 생명을 위협할 정도의 대량출혈이다. 우리는 태아 심박동이 관찰되는 상태에서 보존적 치료인 methotrexate와 leucovorin을 사용하였으나 치료에 실패하고 소파수술및 도뇨관을 사용 하여 자궁경부 임신을 성공적으로 치료했음을 보고한다. 착상부위에서의 출혈은 자궁 경부내의 Foley 도뇨관으로 조절하였다. 계속적인 임신을 원하는 자궁경부 임신 환자의 간단하면서 효과적인 치료는 소파수술 후에 자궁경부내에 도뇨관을 넣어서 확장시켜 두는 것이다. Cervical pregnancy is a rarely life-threatening form of ectopic pregnancy in which the blastocyst implants within the cervical mucosa below the internal os of the uterine cervix. The majority of patients with a cervical pregnancy are women with low parity, thus, the current treatment trend is to preserve their reproductive function. The main problem of conservative surgical treatment is life-threatening hemorrhage after pregnancy evacuation. We report a case of cervical pregnancy that fail to respond to methotrexate and leucovorin. The fetal heart beat was still detectable, thereafter we successfully treated the cervical pregnancy by dilatation and curettage and Foley catheter ballooning. Hemorrhage from the implantation site was controlled by intracervical placement of a Foley catheter balloon. Placement and inflation of Foley catheter in the cervix after cervical dilatation and curettage appears to be a simple and effective means of managing cervical pregnancy in patient who wishes to retain future fertility potential.