지주막하 출혈을 동반한 용혈성 요독 증후군 1례

김웅흠,김우택,이창연,김동욱,박성기,장무환 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

Hemolytic uremic syndrome is characterized by Coombs-negative microangiopathic hemolytic anemia, thrombocytopenia, and acute renal failure in infant and children. At present, E. coli 0157 : H7 and other verotoxin-producing E. coli(VTEC) seem to be a major cause of this illness. The majority of patients have a prodrome of bloody diarrhea, and acute renal failure develops several days to weeks later. Although an optimal treatment remains unknown, the general management of hypertention and renal failure with early and frequent dialysis is the mainatay of treatment, and intravenous administration of immunoglobulin G is like to be effective. We experienced a case composed of bloody diarrhea, seizures, coma, and anuria in 1 year old girl who was diagnosed as HUS and recovered renal function completely with slight impairment of cognitive ability and behavioral function. We presented this case with a review of literatures.

흰쥐에 있어서 납과 카드뮴이 Hepatic Microsomal Electron Transport System 활성도에 미치는 영향

김주봉,장성근,김기웅,송용범,김무호 순천향대학교 1994 논문집 Vol.17 No.3

In rats treated with lead nitrate and cadmium chloride we observed a change of he level of hepatic microsomal protein, cytochrome P-450 and ??, and electron transport system for P-450 dependent reaction. The contents of microsomal protein, P-450 and b5, and activity of NADPH-cytochrome C(P-450) reductase were decreased according to the dosage of lead nitrate and cadmium chloride. On the other hand, the activity of NADH-cytochrome ?? reductase was increased according to the dosage of lead nitrate and cadmium chloride. These results suggest that the content and activity of cytochrome dependent mono-oxygenase may be dependent on specificity of substrates, and electron transport occurred through two pathway, that is, NADPH-cytochrome C(P-450) reductase and ?? reductase but NADH-cytochrome ?? reductase was mainly act as a mobile carrier so-called second electron carrier.

환경디자인을 통한 범죄예방에 관한 기초적 연구

鄭武雄,金善弼 단국대학교 1993 論文集 Vol.27 No.-

This study, as one subject of E·B·S(Environmental Behavior Study) to find out relationship between environment and behavior, is a scientific implementation to control or prevent crime through environmental design. This study takes the hypotesis that environment give cues of crime, when latent criminals percept it, raise crime. Also, by the hypotesis, this study takes the theory that can prevent crime with seeking environmental elements to promote crime, changing or improving it. The purpose of this study is to seek design guideline applicable to our houses and site plan by consideration of the theory and the case study of crime prevention through environmental design.

韓國住居文化의 連續性과 變化性에 관한 硏究 : 改良韓屋의 生活樣式과 空間適應性을 中心으로

鄭武雄,金善弼,李容賑 단국대학교 1992 論文集 Vol.26 No.-

This is a study on the cultural change and continuity of Korean residential settlement. The relationship between residential environment and life style is essential issue of this study. 1. There is a recyclic relationship influencing each other between residential environment and life style. 2. The adaptation process between host oulture and migrant culture is conflict → sublimation → assimilation and the relative strength between wants and needs is a very essential element in cultural settlement. 3. The continuity factors in the reformed house are family room of Anbang, floor panel heating system(Ondol), inner court of life space, veranda floor, place hierarchy in daily life and preference of main floor(Maru) and texture. 4. The changing factors in the reformed house are influenced by daily life needs and reconstruction, extension, equipment improvement, material exchange and some addition facilities. 5. Soccio-cultural control system is necessary to control the speed, frequency and scale of changing tendency.

다발성 말초신경병증 증상으로 관찰된 폐소세포암의 척수내 전이

박종규,김태웅,송인욱,안무영 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.2S

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Paraquat 중독환자의 초기검사로서 sodium dithionite를 이용한 소변내 paraquat검출의 임상적 의의

윤갑준,임경수,이진웅,김영식,이부수,박덕우,김선만,이강현,황성오,안무업 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Mortality from paraquat intoxication depends upon plasma paraquat concentration. To know the severity of paraquat intoxication is important for directing therapeutic modality and predicting prognosis. Sodium dithionite test for urinary paraquat provides an easy and simple method to determine the severity of paraquat intoxication in emergency department. purpose : To determine whether the result urinary paraquat test by sodium dithionite can predict outcome in patients with paraquat intoxication in emergency department. Subjects : 48 patients(male 31, female 17, mean age 37 years) who had exposure to paraquat and presented within 24 hours after exposure. Result : Thirty five patients were positive in paraquat urine test and thirteen patients were negative. Clinical manifestations were more severe in positive patients than in negatives. Complication was much more in positives than in negatives. 28 of 35 patients(80%) in positives and 2 of 13 patients(15%) in negatives died. Conclusion : Positive test for urinary paraquat is associated with high mortality and morbidity from paraquat intoxication, and qualitative test for urinary paraquat by sodium dithionite is an useful method to determine the severity of paraquat intoxication in emergency department.

Predicting the Interactome of <i>Xanthomonas oryzae </i> pathovar oryzae for target selection and DB service

Kim, Jeong-Gu,Park, Daeui,Kim, Byoung-Chul,Cho, Seong-Woong,Kim, Yeong Tae,Park, Young-Jin,Cho, Hee Jung,Park, Hyunseok,Kim, Ki-Bong,Yoon, Kyong-Oh,Park, Soo-Jun,Lee, Byoung-Moo,Bhak, Jong BioMed Central 2008 BMC bioinformatics Vol.9 No.-

<P><B>Background</B></P><P>Protein-protein interactions (PPIs) play key roles in various cellular functions. In addition, some critical inter-species interactions such as host-pathogen interactions and pathogenicity occur through PPIs. Phytopathogenic bacteria infect hosts through attachment to host tissue, enzyme secretion, exopolysaccharides production, toxins release, iron acquisition, and effector proteins secretion. Many such mechanisms involve some kind of protein-protein interaction in hosts. Our first aim was to predict the whole protein interaction pairs (interactome) of <I>Xanthomonas oryzae </I>pathovar oryzae (Xoo) that is an important pathogenic bacterium that causes bacterial blight (BB) in rice. We developed a detection protocol to find possibly interacting proteins in its host using whole genome PPI prediction algorithms. The second aim was to build a DB server and a bioinformatic procedure for finding target proteins in Xoo for developing pesticides that block host-pathogen protein interactions within critical biochemical pathways.</P><P><B>Description</B></P><P>A PPI network in Xoo proteome was predicted by bioinformatics algorithms: PSIMAP, PEIMAP, and iPfam. We present the resultant species specific interaction network and host-pathogen interaction, XooNET. It is a comprehensive predicted initial PPI data for Xoo. XooNET can be used by experimentalists to pick up protein targets for blocking pathological interactions. XooNET uses most of the major types of PPI algorithms. They are: 1) Protein Structural Interactome MAP (PSIMAP), a method using structural domain of SCOP, 2) Protein Experimental Interactome MAP (PEIMAP), a common method using public resources of experimental protein interaction information such as HPRD, BIND, DIP, MINT, IntAct, and BioGrid, and 3) Domain-domain interactions, a method using Pfam domains such as iPfam. Additionally, XooNET provides information on network properties of the Xoo interactome.</P><P><B>Conclusion</B></P><P>XooNET is an open and free public database server for protein interaction information for Xoo. It contains 4,538 proteins and 26,932 possible interactions consisting of 18,503 (PSIMAP), 3,118 (PEIMAP), and 8,938 (iPfam) pairs. In addition, XooNET provides 3,407 possible interaction pairs between two sets of proteins; 141 Xoo proteins that are predicted as membrane proteins and rice proteomes. The resultant interacting partners of a query protein can be easily retrieved by users as well as the interaction networks in graphical web interfaces. XooNET is freely available from .</P>

Effect of a PMR1 Disruption on the Processing of Heterologous Glycoproteins Secreted in the Yeast Saccharomyces cerevisiae

Kim, Moo-Woong,Ko, Su-Min,Kim, Jeong-Yoon,Sohn, Jung-Hoon,Park, Eui-Sung,Kang, Hyun-Ah,Rhee, Sang-Ki The Korean Society for Biotechnology and Bioengine 2000 Biotechnology and Bioprocess Engineering Vol.5 No.4

The Saccharomyces cerevisiae PMR1 gene encodes a Ca2+-ATPase localized in the Golgi. We have investigated the effects of PMR1 disruption in S. cerevisiae on the glycosylation and secretion of three heterologous glycoproteins, human ${\alpha}$1-antitrypsin (${\alpha}$1-AT), human antithrombin III (ATHIII), and Aspergillus niger glucose oxidase (GOD). The pmr1 null mutant strain secreted larger amounts of ATHIII and GOD proteins per a unit cell mass than the wild type strain. Despite a lower growth rate of the pmr1 mutant, two-fold higher level of human ATHIII was detected in the culture supernatant from the pmr1 mutant compared to that of the wild-type strain. The pmr1 mutant strain secreted ${\alpha}$1-AT and the GOD proteins mostly as core-glycosylated forms, in contrast to the hyperglycosylated proteins secreted in the wild-type strain. Furthermore, the core-glycosylated forms secreted in the pmr1 mutant migrated slightly faster on SDS-PAGE than those secreted in the mnn9 deletion mutant and the wild type strains. Analysis of the recombinant GOD with anti-${\alpha}$1,3-mannose antibody revealed that GOD secreted in the pmr1 mutant did not have terminal ${\alpha}$1,3-linked mannose unlike those secreted in the mnn9 mutant and the wild type strains. The present results indicate that the pmr1 mutant, with the super-secretion phenotype, is useful as a host system to produce recombinant glycoproteins lacking high-mannose outer chains.

HCV, Acute, LT : The Clinico-Pathological Features of Drug Induced Liver Injury

( Woong Cheul Lee ),( Jae Young Jang ),( Jin Nyoung Kim ),( Soung Won Jeong ),( Eui Ju Park ),( Byoung Moo Lee ),( Yun Nah Lee ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background: Drug-induced liver injury (DILI) has recently increased and is one of major cause of acute hepatitis. We investigated the clinico-pathological findings in patients with DILI whom liver biopsy was conducted. Methods: A retrospective analysis was performed using medical records of sixty five cases of DILI that were selected based on a modified RUCAM (Roussel Uclaf Causality Assessment Method) score of 7 or above. Results: The incidence was higher in women (n=41) than men (n=24). The age (mean±SD) of the patients was 48.2±13.1 years. The major causes of the DILI included the use of herbal medications (43.2%), prescription medications (21.6%) and traditional therapeutic preparations and dietary supplements (35%). At the time of admission, various symptoms (anorexia and abdominal discomfort (46.2%), jaundice and itching (40%), myalgia and fatigue (32.3%), fever and chill (16.9%), headache and dizziness (6.2%)) were presented. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, alkaline phosphatase (ALP) and γ-glutamyl transpeptidase peak levels were 650.1±685.2 IU/L, 855.7±780.9 IU/ 10±9.5 mg/dL, 371±454 IU/L and 247.9±227.2 IU/L, respectively. The mean modified RUCAM score of the patients was 8.01±0.75. The types of liver injury according to R ratio ([ALT/ UNL]/[ALP/UNL]) were hepatocellular type (67.7%), mixed type (10.8%) and cholestatic type (21.5%). The main pathologic findings were zone 3 necrosis and swelling (53.8%), increased eosinophilic infiltration (43.1%) and bridging necrosis (21.5%). Herbal medications and traditional therapeutic preparations were associated with hepatocellular type`s liver injury compared to prescription medications (P<0.002). All patients were recovered without complication. Conclusions: The clinico-pathologic findings of DILI were diverse. Zone 3 necrosis and swelling was most common pathologic feature. The type of liver injury with herbal medications and traditional therapeutic preparations revealed hepatocellular type.

LC : Renal Function Predicts 14 Days Mortality in Cirrhotic Patient with Spontaneous Bacterial Peritonitis; Multicenter Retrospective Study

( Woong Cheul Lee ),( Jae Young Jang ),( Young Don Kim ),( Yun Nah Lee ),( Young Sin Cho ),( Jin Nyoung Kim ),( Eui Ju Park ),( Byoung Moo Lee ),( Soung Won Jeong ),( Sae Hwan Lee ),( Sang Gyune Kim ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background: Spontaneous bacterial peritonitis (SBP) is one of severe complications in liver cirrhosis. Early detection of high-risk patients is essential for prognostic improvement. We investigate the predictive factors related with 14 days mortality in patient with SBP. Methods: We conducted a retrospective study of two hundred seventy five SBP patients admitted in four tertiary referral hospitals between August 2002 and February 2013. We collect patients` laboratory and radiologic data from medical record. We calculated Child-Turcotte-Pugh score (CTP), Model for End- Stage Liver Disease-Na (MELD-Na) score using the laboratory data at the time of SBP occurrence. Results: The mean age of the patients was 56.9 years (213 male, 62 female). The cause of liver cirrhosis was hepatitis B (44%), alcohol (41.8%) and hepatitis C (6.9%). Mean score of MELD-Na and CTP score were 27.44 and 10.67, respectively. The patients who die from SBP within 14 days were 17.8% (n=49). The statistically significant factor of multivariate analysis were baseline MELD-Na (P=0.002) and GFR (P=0.034). Two hundred thirty three patients who could follow-up were analyzed. Baseline MELD-Na (P=0.027) and maximal creatinine level during the follow-up (P=0.02) were significantly associated with 14 days mortality. The prognostic accuracy of baseline MELD-Na, initial GFR and maximal creatinine during the follow-up were 75.6%, 74.0% and 78.0%, respectively (P<0.001). The cut-off values were 27 (baseline MELD-Na), 35.9 mL/min (initial GFR) and 2 mg/dL (maximal creatinine), respectively (P<0.001). Conclusions: Renal function was important predictive factor of 14 days mortality in cirrhotic patients with SBP. The patients with SBP and renal insufficiency should be treated more intensively.