초음파 기관지 내시경으로 진단한 기관기관지 골연골형성증 1예

이천우,옥철호,정만홍,장태원,임성경,조은주,이신준,이혜원,구상건,천봉권 고신대학교 의과대학 2011 고신대학교 의과대학 학술지 Vol.26 No.2

Tracheobronchopathia osteochondroplastica (TO) is a rare benign disease of unknown etiology characterized by accumulation of calcium phosphate in the submucosa of large airways and benign proliferation of bone and cartilage resulting in nodular formation. We report a case of tracheobronchopathia osteochondroplastica diagnosed by Endobronchial ultrasonography in a 56-year-old man. Chest Computed Tomography revealed thickening of tracheal and bronchial wall, and multiple nodules through whole trachea. Endobronchial ultrasonography showed numerous submucosal nodules with hetero-echogenecity in the third and fourth layers. Histopathological examination revealed nonspecific bronchitis with squamous metaplasia and metaplastic ossification. We confirmed tracheobronchopathia osteochondroplastica. The patient's symptoms were successfully treated with antibiotics and oxygen supplyment. endobronchial ultrasonography can helpful diagnosis in tracheobronchopathia osteochondroplastica.

열안정형 카테콜-O-메틸전이효소 유전자의 클로닝

홍경만,최용복,정갑용,지은정,장현신,박현,백문기 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Catechol-O-methyltransferase (COMT; EC 2.1.1.6) is the enzyme which catalyzes the transfer of methyl group to the catecholamine neurotransmitters from its methyl donor S-adenosyl-L-methionine. Partially purified COMT from rat liver has been used to measure the concentration of catecholamines in the blood through solvent extraction and thin layer chromatography after converting ^3H-methyl derivatives of catecholamines. To improve this inconvenient and fluctuating method, an attempt was made to use COMT gene for the measurement of catecholamines. Specific primers, COMT5P (5'-TGC TCA GAG GTG CTT TGA AG-3') and COMT3P (5'-GGA GCC GCA GAA GGT CA G-3'), were used to amplify COMT gene from human placenta cDNA library. The amplified COMT gene through 35 cycles of polymerase chain reaction was cloned into T-vector and the nucleotide sequences are determined by automatic sequencer. Human COMT has two common variants, a thermostable high activity form which has valine at amino acid 158 and a thermolabile low activity form which Has methionine at the same position. The cloned COMT gene in this study has both variants. Clone 4 and 5 have valine at amino acid 158 (guanine at nucleotide 472) and clone 1-3 have methionine at this site (adenine at nucleotide 472). In addition to this, there are other DNA polymorphisms in COMT gene at nucleotide 101, 102 (amino acid 34, a structural mutation cysteine/serine) and nucleotide 186 (a silent mutation). All of the clones have cysteine at amino acid 34 (guanine and adenine at nucleotides 101 and 102) and cytosine at nucleotide 186 except clone 3 which has thymine at this nucleotide. Clone 4 and 5 are thermostable high activity variants, suggesting being more useful for the measurement of catecholamines after expressing the gene.

[논문] trn L-trn F에 의한 한국 족도리풀속 식물 종 분류

성혜경,허만규,허홍욱 釜山大學校 師範大學 科學敎育硏究所 2011 科學敎育硏究報 Vol.38 No.-

Asarum consists low-growing herbs and is a genus in the Aristo-lochiaceae family with species found in the north temperate zones with most species in Asia. Any species with a highly structured variation pattern must show different distributions of phenotypic characteristics at different locations. I analysed intra- and interspecific phylogenic relationships within genus Asarum in Korea. I evaluated a representative sample of the fourteen taxa (eight Korean taxa and six obtained taxa from GenBank) with the trn L intron and the trn L - trn F spacer region of the chloroplast genome to estimate genetic relationships within genus Asarum. No variable positions were found within the trn L 5` exon and trn L 3` exon. Variable positions within the intron region were mostly distributed outside the structural elements required for intron processing, i.e., not within the first 140 bp at the 5` end and not within the 90 bp at the 3` end of the intron region. Within the spacer region, variable positions were evenly distributed. Alignment of the DNA sequences required adding numerous gaps. Sequence variation within the Asarum is mostly due to nucleotide substitutions, although several small indels and 20 inserts can be found. Another source of sequence divergence is length variation due to stretches of short repeats that occur at the intergenic spacer region ITS2 in all the Asarum. A + T content for eight Korean species of genus Asarum ranged between 64.3% and 64.6%. The base furtherance did not showed the significant difference to the by a total taxon. These values are similar to those (64.5%~67.1%) for the angiosperm alignments of the total trn L and trn F region. The main phylogenetic analysis revealed two distinct clades. The first clade includes ten species (eight Korean species, A. nipponicum, and A. tamaensis). The second clade includes four species (A. canadense , A. cautatum, A. chingchengense , and A. yakusimense). However, some internal nodes are poorly supported. Within Korean Asarum, four species were unsolved portions.

Expression of a Protein-Arginine N-Methyltransferase, HRMT1L1

Hong, Kyeong-Man,Park, Hyun,Paik, Moon-Kee 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Protein methylase Ⅰ (or protein-arginine N-methyltransferase) catalyzes the methylation of arginine residues in glycine-arginine-rich (GAR) domains of eucaryotic proteins. Members of this family of methyl accepting substrates include hnRNP A1, nucleolin and fibrillarin Mammalian protein methylase Ⅰ genes, PRMT1, HRMT1L1 and PRMT3, were cloned recently. But the biological function of these enzymes is not clear yet, so it would be necessary to produce monoclonal antibodies against protein methylase Ⅰ enzymes for the study of their function. In this study, HRMT1L1, a human protein methylase Ⅰ gene, was cloned and was expressed in bacteria and the recombinant protein was purified for the production of monoclonal antibody. Total human HRMT1L1 cDNA was amplified by PCR from human placental cDNA library. The BamHI site was included in 5' sequence of the 5' primer. The amplified PCR product was cloned to T-vector and sequenced to confirm the nucleotide sequence. The HRMT1L1-T-vector was digested with BamHI and EcoRI and the HRMT1L1 cDNA was cloned to Trx fusion expression vector and was sequenced again. Transformed bacteria with Trx-HRMT1L1 vector was induced to Trx-HRMT1L1 fusion protein, The induced fusion protein was purified with His-bind chromatography and was digested with recombinant enterokinase. The digested proteins were passed through His-bind column to remove TrxA protein. The purified HRMT1L1 recombinant protein will be used to produce monoclonal antibody.

Cloning and Expression of Protein-arginine Methyltransferase Ⅰ

Hong, Kyeong-Man,Choi, Yong-Bock,Chang, Yoo-Jung,Kim, Han-Soo,Lee, Sang-Do,Pak, Gun-Suk,Kim, Jeong-Kyu,Rhee, Kang-Ill,Park, Hyun,Kim, Kyung-Suk,Park, Moon-Kee 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.2

Purpose: PRMT I gene was cloned recently, but the biological function of these enzymes has not been clear yet. This study was designed to produce recombinant PRMT1 in Escherichia coli for the future production of monoclonal antibody. Methods: Partial human PRMT1 cDNA was amplified by PCR from human placental cDNA library. The hPRMT1 was cloned to T-vector, again to Trx fusion vector for expression. The transformed bacteria with Trx-hPRMT1 vector were induced. The induced fusion protein was purified with His-bind chromatography and was digested with recombinant enterokinase. Digested proteins were passed through His-bind column to remove TrxA protein. Results: The recombinant fusion protein, Trx-hPRMT1, was expressed in E.coli. And the pure recombinant hPRMT1 was purified from His-bind chromatography and the digestion with enterokinase. Conclusions: The purified recombinant hPRMT1 protein was adequate to use for the ^production of monoclonal antibody.

Inactivation of LLC1 gene in nonsmall cell lung cancer

Hong, Kyeong-Man,Yang, Sei-Hoon,Chowdhuri, Sinchita Roy,Player, Audrey,Hames, Megan,Fukuoka, Junya,Meerzaman, Daoud,Dracheva, Tatiana,Sun, Zhifu,Yang, Ping,Jen, Jin Alan R. Liss, Inc 2007 International journal of cancer Vol.120 No.11

<P>Serial analysis of gene expression studies led us to identify a previously unknown gene, c20orf85, that is present in the normal lung epithelium but absent or downregulated in most primary nonsmall cell lung cancers and lung cancer cell lines. We named this gene LLC1 for Low in Lung Cancer 1. LLC1 is located on chromosome 20q13.3 and has a 70% GC content in the promoter region. It has 4 exons and encodes a protein containing 137 amino acids. By in situ hybridization, we observed that LLC1 message is localized in normal lung bronchial epithelial cells but absent in 13 of 14 lung adenocarcinoma and 9 out of 10 lung squamous carcinoma samples. Methylation at CpG sites of the LLC1 promoter was frequently observed in lung cancer cell lines and in a fraction of primary lung cancer tissues. Treatment with 5-aza deoxycytidine resulted in a reduced methylation of the LLC1 promoter concomitant with the increase of LLC1 expression. These results suggest that inactivation of LLC1 by means of promoter methylation is a frequent event in nonsmall cell lung cancer and may play a role in lung tumorigenesis. © 2007 Wiley-Liss, Inc.</P>

Clinical Characteristics and Treatment Outcomes in Patients with Relapsing Polychondritis involving Airways

( Gooh Yeon Hong ),( Kyung Jong Lee ),( Kyeong Man Jeon ),( Won Jung Koh ),( Gee Young Suh ),( Man Pyo Chung ),( O Jung Kwon ),( Ho Joong Kim ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Objectives: To investigate the clinical characteristics, management modalities and outcomes in patients with relapsing polychondritis (RP) involving airways. Methods: Medical records of RP patients involving airways were collected at the Samsung Medical Center from August 2004 to December 2011. Clinical manifestations including rheumatologic record, diagnostic tests, treatment modalities and clinical outcomes were retrospectively investigated. Results: Twelve patients (5 women and 7 men) with a median age of 48 yr (IQR, 44-60 yr) were included. All patients had airway involvement including trachea (100%), main bronchi (83%) and larynx (25%). Rheumatologic manifestation were frequent including inflammatory arthritis (50%), auricular chondritis (42%), keratoconjuntivitis(42%), nasal chondritis (42%), saddle nose (25%) and sensorineural hearing loss (17%). All patients were treated with high-dose corticosteroids (1,000 mg per day) for acute exacerbation and maintained with oral prednisolone (5-40 mg per day) with weekly methotrexate (2.5-15 mg per week) during follow up. Among the 12 patients, mechanical ventilation was required in 1 patient. Currently, 9 patients have survived without ventilator support and 8 patients without tracheostomy. Two patients underwent tracheostomy with endobronchial stenting. During follow up (median, 24months [IQR 7-50]), clinical outcome was favorable in 9 patients, but 3 patients died of pneumonia and respiratory failure. Conclusions: High doses of corticosteroids on acute exacerbation followed by maintenance-dose prednisolone with methotrexate could be recommended as a therapeutic option in patients with RP involving airways. Airway intervention is sometimes required in experienced hands.

고요산혈증을 보이는 한국인 건강검진 내원자들에서의 통풍의 유병률과 관련 인자들에 관한 연구

김응호 ( Eung Ho Kim ),전경만 ( Kyeong Man Jeon ),박건우 ( Keon Woo Park ),김형진 ( Hyung Jin Kim ),안중경 ( Joong Kyong Ahn ),전찬홍 ( Chan Hong Jeon ),차훈석 ( Hoon Suk Cha ),고은미 ( Eun Mi Koh ) 대한류마티스학회 2004 대한류마티스학회지 Vol.11 No.1

Objective: To investigate the prevalence of gout among hyperuricemic Korean people who visited Health Screening Center and to study related factors in the development of gout. Methods: The medical records of 6,461 people who visited Health Screening Center from March to May in 2002 were reviewed. Five hundred twenty one out of 603 hyperuricemic individuals were interviewed by telephone. Gout was diagnosed according to the clinical criteria of Wallace. Basic demographic and lifestyle variables as well as biochemical data were collected. Results: The prevalence of hyperuricemia was 14.3% in men and 2.2% in women. The prevalence of gout among hyperuricemic population was 16.6% for men and 6.7% for women. The mean age was higher in gout patients compared to the hyperuricemic people without gout, and serum uric acid and creatinine levels were increased in gout patients. The mean systolic blood pressure and the alcohol consumption amount were also increased in gout patients. In the multiple logistic regression analysis, statistically significant related factors for the development of gout among hyperuricemic population were age, uric acid level and alcohol consumption amount. Conclusion: The prevalence of gout among hyperuricemic Korean people who visited Health Screening Center was 16.6% for men and 6.7% for women. The possible related factors in the development of gout among hyperuricemic population were age, uric acid level and alcohol consumption amount.

Preparation of Mesoporous CuCe-Based Ternary Metal Oxide by Nano-Replication and Its Application to Decomposition of Liquid Monopropellant

Hong, Sunghoon,Heo, Sujeong,Li, Chengbin,Jeon, Bo Kyeong,Kim, Ji Man,Jo, Young Min,Kim, Wooram,Jeon, Jong-Ki American Scientific Publishers 2018 Journal of nanoscience and nanotechnology Vol.18 No.2

<P>Mesoporous CuCe-based ternary metal oxides were synthesized using KIT-6 as a hard template through a nano-casting method. The mesoporous CuCe-based metal oxides were applied to the catalytic decomposition of the ammonium dinitramide-based liquid monopropellant. The decomposition onset temperature over the meso-CuCe ternary metal oxides was much lower than that over the CuCeOx catalyst prepared by conventional precipitation method. Higher activity of the meso-CuCe ternary metal oxides is attributed to higher surface area and larger pore size of the meso-CuCe ternary metal oxides than those of the conventional CuCe oxide. The highest activity of meso-CuCeZr catalyst among the meso-CuCe ternary metal oxide catalysts is likely due to the highest mesoporosity.</P>

Cdk1-cyclin B1-mediated phosphorylation of tumor-associated microtubule-associated protein/cytoskeleton-associated protein 2 in mitosis.

Hong, Kyung Uk,Kim, Hyun-Jun,Kim, Hyo-Sil,Seong, Yeon-Sun,Hong, Kyeong-Man,Bae, Chang-Dae,Park, Joobae American Society for Biochemistry and Molecular Bi 2009 The Journal of biological chemistry Vol.284 No.24

<P>During mitosis, establishment of structurally and functionally sound bipolar spindles is necessary for maintaining the fidelity of chromosome segregation. Tumor-associated microtubule-associated protein (TMAP), also known as cytoskeleton-associated protein 2 (CKAP2), is a mitotic spindle-associated protein whose level is frequently up-regulated in various malignancies. Previous reports have suggested that TMAP is a potential regulator of mitotic spindle assembly and dynamics and that it is required for chromosome segregation to occur properly. So far, there have been no reports on how its mitosis-related functions are regulated. Here, we report that TMAP is hyper-phosphorylated at the C terminus specifically during mitosis. At least four different residues (Thr-578, Thr-596, Thr-622, and Ser-627) were responsible for the mitosis-specific phosphorylation of TMAP. Among these, Thr-622 was specifically phosphorylated by Cdk1-cyclin B1 both in vitro and in vivo. Interestingly, compared with the wild type, a phosphorylation-deficient mutant form of TMAP, in which Thr-622 had been replaced with an alanine (T622A), induced a significant increase in the frequency of metaphase cells with abnormal bipolar spindles, which often displayed disorganized, asymmetrical, or narrow and elongated morphologies. Formation of these abnormal bipolar spindles subsequently resulted in misalignment of metaphase chromosomes and ultimately caused a delay in the entry into anaphase. Moreover, such defects resulting from the T622A mutation were associated with a decrease in the rate of protein turnover at spindle microtubules. These findings suggest that Cdk1-cyclin B1-mediated phosphorylation of TMAP is important for and contributes to proper regulation of microtubule dynamics and establishment of functional bipolar spindles during mitosis.</P>