Comparison of Proliferative Activity in Each Histological Subtypes of Benign and Atypical Intracranial Meningiomas by PCNA and Ki-67 Immunolabeling

최승진,장은덕,권성오,계대곤,박춘근,이상원,강준기,Choi, Seung Jin,Chang, Eun Deok,Kwon, Seung Oh,Kye, Dae Kon,Park, Choon Keun,Lee, Sang Won,Kang, Joon Ki The Korean Neurosurgical Society 2000 Journal of Korean neurosurgical society Vol.29 No.9

목 적 : 양성 뇌수막종에 비하여 이형성 및 악성 뇌수막종이 나쁜 임상적인 예후 및 양상을 보이는 것은 잘 알려져 있으나, 양성 뇌수막종에 있어서 각각의 병리조직학적 아형에 따른 생물학적 양상의 차이에 대해서는 잘 알려지지 않거나 일부 논란이 되고있다. 본 연구에서는 이형성 뇌수막종 및 양성 뇌수막종의 각각의 병리조직학적 아형에 따른 증식능의 차이여부를 알아보고자 PCNA와 Ki-67표지지수를 분석하였다. 방 법 : 본원에서 뇌수막종으로 수술을 시행하여 얻은, 재발을 보여 재수술을 시행한 2례를 포함하여, 파라핀에 포매시킨 27개의 조직을 대상으로 병리학적인 증식능을 분석하기 위해, PCNA에 대한 단일항체 및 MIB-1 단일항체를 이용한 면역조직화학적 염색을 시행하였다. 조직학적 분류상 meningothelial type이 8례, transitional type이 9례, fibroblastic type이 5례였으며, 이형성 수막종이 5례였다. 결 과 : PCNA표지지수의 평균값은 양성 수막종에서 meningothelial type이 $4.82{\pm}5.10%$, transitional type이 $9.01{\pm}4.25%$, fibroblastic type이 $5.66{\pm}5.32%$를 보였으나 이형성 수막종에서는 $27.62{\pm}19.67%$의 높은 지수를 나타냈고, Ki-67 표지지수의 평균값은 양성 수막종의 아형에서 각각 $0.43{\pm}0.85%$, $0.44{\pm}1.08%$, $0.24{\pm}0.18%$를 보이고, 역시 이형성 수막종에서는 $0.84{\pm}0.59%$의 높은 지수를 보였다. 즉, 양성 수막종에서 각각의 아형에 따른 PCNA 및 Ki-67 표지지수는 통계학적으로 의미있는 차이는 없었으나(p>0.05), 이형성 수막종에서는 의미있는 높은 표지지수를 보여(p<0.05) 양성 수막종에서 보다 높은 증식능을 보임을 알 수 있었다. 결 론 : PCNA 및 Ki-67 표지지수를 이용한 증식능의 비교결과, 양성 뇌수막종에서는 각각의 아형에 따른 생물학적 양상이나 예후는 차이가 없을것으로 생각되나, 이형성 수막종에서는 높은 증식능을 보여 이에 대한 예후를 예상할 수 있을것으로 생각되며, 또한 이러한 표지지수가 병리조직학적으로 양성과 이형성의 감별에 많은 도움이 될것으로 사료된다. Objective : The clinical prognosis and biological behavior of atypical and especially malignant meningiomas are well known to be worse than benign meningioma, but the degree of biological aggressiveness in each classical subtypes of benign meningioma is controversy. This study was performed to see whether there is a difference in the proliferative activity between each different histological subtypes of benign meningioma as well as atypical meningioma. Methods : Paraffin-embedded surgical specimens of 27 meningiomas, including two recurrent tumors, were studied to evaluate proliferative activity by immunohistochemical method with monoclonal antibodies to proliferating cell nuclear antigen(PCNA) and MIB-1. The specimens consisted of 8 cases of meningothelial, 9 cases of transitional, 5 cases of fibroblastic subtypes and 5 cases of atypical meningiomas. Results : Mean PCNA labeling indices of meningothelial, transitional and fibroblastic meningiomas were $4.82{\pm}5.10%$, $9.01{\pm}4.25%$ and $5.66{\pm}5.32%$, but that of atypical meningiomas was $27.62{\pm}19.67%$, noting a higher value compared to all three subtypes of benign meningiomas. Mean Ki-67 labeling indices of the above 3 subtypes were $0.43{\pm}0.85%$, $0.44{\pm}1.08%$ and $0.24{\pm}0.18%$, and that of atypical meningiomas was also revealed to be of higher value ($0.84{\pm}0.59%$). PCNA and Ki-67 labeling indices were not statistically different between histological subtypes of benign meningioma(p>0.05), but the differences of both immunolabeling between benign and atypical meningiomas were statistically significant(p<0.05). Conclusion : Immunolabeling of PCNA and Ki-67 in intracranial meningiomas reveals no prognostic difference between meningothelial, transitional and fibroblastic subtypes in classical benign meningiomas by measuring expression of PCNA and Ki-67, but it seems to be helpful in differentiating benign and atypical meningioma, later showing more proliferative activity and biological aggressiveness.

역행적 골수정 삽입술을 이용한 대퇴골 과상부 골절의 치료

권진우,신승호,조원호,성기호,김기환,손경태,이상훈,박세원,배종문,권태우 대한골절학회 2003 대한골절학회지 Vol.16 No.4

Biosimilar Development And Case Study in Celltrion

Ki?Sung Kwon 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

1930년대 야담잡지 소재 연성흠의 야담 기술과 의미

권기성(Kwon, Ki-Sung) 우리문학회 2023 우리文學硏究 Vol.- No.79

1920년대부터 야담은 동화와 긴밀히 접촉하고 있었다. 다만 그 방법은 동화가 야담을 활용하는 정도였으며, 연구자의 관심도 여기에서 벗어나지 않았다. 그런데 1930년대부터 야담 잡지의 기획자는 동화작가들을 소환하여 그들이 지닌 소재의 유사성 등을 활용하여 야담을 작성하도록 한다. 그 차이를 살피기 위해 본고에서는 연성흠이 『월간야담』과 『야담』 잡지에 남긴 작품에 주목했다. 연성흠의 야담은 대개 전시기 야담집에 수록된 작품을 준용하는 특징을 보이지만, 필요한 경우가 있다면 적극적 변개도 주저하지 않았다. 그리고 이런 편찬과 서술의 특징은, 한결같이 선한 인물의 시혜와 보은이라는 지점을 강조하면서 ‘공동체 윤리’를 강조한 주제적 지향을 견인했다. 이는 그의 동화작법과 퍽 유사한 것이었다. 한편 야사를 토대로 새로운 이야기를 만들어낼 때는 통속과 비극이라는 당대의 ‘애사’ 글쓰기를 답습하는 경향도 보였다. 곧 야담과 야사의 글쓰기 방식이 미세하게 달랐던 셈이다. 논문에 제시된 연성흠의 작품을 통해 두 잡지의 기획이 다양한 형태로 이루어졌으며, 독자를 유인하는 기획의 소산이었음을 짐작해 볼 수 있었다. 이는 곧 야담 집필진 저변의 다양화를 통해 활로를 모색한 가운데, 아동문학과의 접속이 주요한 역할을 했던 것이다. 그 저변에는 지면의 확대에 따른 다양한 글쓰기의 가능, 개인의 경제적 사정, 친분 등 여러 이유가 있었다. 결과적으로 30년대 동화작가들이 야담 잡지에 참여하고 다양한 야담 기법을 시도하면서, 당시 야담의 흥행을 견인하는 일익을 담당하고 있었던 것이라 할 수 있다. Since the 1920s, Yadam has been in close contact with fairy tales. However, the method was to the extent that fairy tales used night stories, and the researcher’s interest did not deviate from this. However, from the 1930s, the organizers of Yadam magazine summoned fairy tale writers to write Yadam by utilizing the similarity of their materials. In order to examine the difference, this paper focused on the works left by Yeon Sung-heum in 『Wolgan-Yadam』 and 『Yadam』 magazines. Yeon Sung-heum’s Yadams usually show the characteristics of applying mutatis mutandis to the works contained in the exhibition’s Yadams, but he did not hesitate to actively transform himself if necessary. And these characteristics of compilation and description drove the thematic orientation that emphasized “community ethics” while emphasizing the point of the benefit and complement of good characters. This was very similar to his fairy tale writing. On the other hand, when creating a new story based on wild events, there was also a tendency to follow the writing of the “Sad-history” of the time of popularity and tragedy. In other words, the writing methods of Yadam and Yasa were slightly different. Through the work of Yeon Seong-heum presented in the paper, it was possible to guess that the planning of the two magazines was carried out in various forms, and that it was the product of the planning that attracted readers. In other words, the connection with children’s literature played a major role while seeking a way out through the diversification of the base of the Yadam writers. There were various reasons at the base, such as the possibility of various writing due to the expansion of the space, individual economic circumstances, and friendship. As a result, it can be said that in the 30s, fairy tale writers participated in Yadam magazines and tried various Yadam techniques, playing a role in driving the success of Yadam at that time.

中風病辨證診斷標準에 의한 臨床的 硏究

任長信,文祥官,高昌男,權正男,曺基湖,金永錫,裵亨燮,李京燮 경희대학교 1997 慶熙醫學 Vol.13 No.3

불면을 호소하는 뇌실질내출혈 환자 치험 1례

김경묵,김세원,서유나,정우상,문상관,진철,조기호,권승원,Kim, Gyeong-muk,Kim, Se-won,Seo, Yu-na,Jung, Woo-sang,Moon, Sang-Kwan,Jin, Chul,Cho, Ki-ho,Kwon, Seungwon 대한중풍순환신경학회 2019 대한중풍.순환신경학회지 Vol.20 No.1

A case of a 46-year-old Korean male with post-stroke insomnia that induced by intracranial hemorrhage was presented. He was treated with Transcutaneous electric acupoint stimulation (TEAS) at BL62 and KI6 once per a day for 17 days. We recorded the patient's sleep time and time of asleep, and used PSQI-K to assess severity of patient's insomnia symptom. After using TEAS, there was improvement in patient's insomnia severity and the time of sleep. From the result of this case, electrical stimulation at acupoint can may be effective in treating post-stroke insomnia.

양 족부 열감을 호소하는 하지불안증후군 환자 치험 1례

김경묵,서원주,김수빈,정우상,문상관,권승원,조기호,Kim, Gyeong-muk,Suh, Won-joo,Kim, Su-bin,Jung, Woo-sang,Mun, Sang-kwan,Kwon, Seung-won,Cho, Ki-ho 대한한방내과학회 2018 大韓韓方內科學會誌 Vol.39 No.2

Objectives: The purpose of this study is to evaluate the effectiveness of electroacupuncture on heat sensation of the feet of a 61-year-old female patient with restless leg syndrome. Methods: Electroacupuncture was performed at four acupoints (KI3 (太谿), KI6 (照海), LR3 (太衝), LR2 (行間)) selected to reduce heat sensation in both feet. The patient's symptoms were assessed using the VAS (Visual Analogue Scale), the SF-MPQ (short-form McGill Pain Questionnaire), and the IRLS (International Restless Legs Scale). The treatment was performed from April 20, 2010 to April 24, 2010. Results: During five sessions of electroacupuncture treatments, the VAS, SF-MPQ, and IRLS scores improved throughout the treatment period and afterwards. Conclusions: The results suggest that electroacupuncture at KI3(太谿), KI6(照海), LR3(太衝), LR2(行間) can be effective for reducing hot flush in both feet.

韓國産 大豆油의 抗酸化에 關한 硏究(第一報) : 大豆油에 있어서 tocopherol과 phospholipid의 抗酸化 相乘效果에 關하여 Synergistic Antioxidation Effect of Tocopherol and Phospholipid

權泰鳳,吳成基 慶熙大學校 1985 論文集 Vol.14 No.-

The synergistic antioxidation effect of α-tocopherol and soybean phospholipid was examined by measuring peroxide value at 100℃, 150℃ and 200℃ with time. Various concentrations of tocopherol and phospholipid were added to the edible soybean oil respectively. When phospholipid was not added the antioxidation effect of tocopherol was more found at 0.01% addition level than 0.05% or 0.1% additional level. In the case of phospholipid was added the antioxidation effect was more significantly found than phospholipid not added and, when the addition level of tocopherol was increased the antioxidation effect of phospholipid was found at higher phospholipid addition level. But at the low addition level of tocopherol, the high phospholipid addition level was decreased antioxidation effect. When the 0.05% tocopherol and 0.1% phospholipid was added to the edible sobean oil at 100℃, the antioxidation effect was exhibited to maximum and also, 0.05% tocopherol and 0.05% phospholipid at 150℃ too. The antioxidation effect was increased with an increase phospholipid addition level at 100℃ but at the low addition level of topherol, the antioxidation effect was decreased with an increase phospholipid concentrations at 150℃. The antioxidation effect was not significantly different from phospholipid and tocopherol at 200℃

Cinnamonitrile의 酸 加水分解 反應(Ⅰ)

成洛道,權寄星 圓光大學校 基礎自然科學硏究所 1982 基礎科學硏究誌 Vol.1 No.1

The rate constants for the hydrolysis of cinnamonitrile at pH 0.0~7.0 range are determined by UV spectrophotometry in 50% methanol at 25℃. Analysis of rate equation and the general base catalysis, in the hydrolysis of cinnamonitrile therefore it appears probable that the attack by a nucleophile, water molecule occurs at the increased positive charge α-carbone atom and the αC-βC bond scission follow subsequently in acidic and neutral media. Conformation of (E)-cinnamonitrile have been studied by MO theoretically using extended Hu¨ckel theory(EHT) and CNDO/2 molecular orbital calculation methods. The results show that the most preferable conformation of the phenyl ring rotamer is the coplaner forms with regard to the C_7(α)=C_8(β)plane.

Ethylcinnamate에 對한 Iso-propylmercaptan의 親核性 添加反應에 關한 反應速度論的 硏究

權奇星,李天培,成洛道 圓光大學校 1977 論文集 Vol.11 No.-

ethylcinnamate에 대한 iso-propylmercaptan의 친핵성 첨가반응 속도상수를 측정하여 넓은 pH 범위에서 잘맞는 반응속도식을 얻었다. 이 식에 의하면 pH 6.5 이하에서는 iso-propylmercaptan과 iso-propylmercaptide ion의 첨가가 경쟁적으로 일어난다. pH 10이상에서는 ethylcinnamate에 iso-propylmercaptide ion의 첨가만이 일어남을 알수 있다. 그러므로 본 실험에서 구한 반응속도식으로부터 실험측정값과 계산 값(이론 값)이 여러 pH를 통하여 잘 맞기 때문에 넓은 pH범위내에서 일어나는 친핵성 첨가반은 mechanism을 잘 설명할 수 있었다. The rate constants of nucleophilic addition reaction of iso-propylmercaptan to ethylcinnamate were determined at various pH and a rate equation which can be applied overwide pH range was obtained; ?? The rate equation reveals that below pH 6.5 the reaction is initiated by addition of iso-propylmercaptan molecule, from pH 6.5 to 10.0 iso-propylmercaptan and iso-propylmercaptide ion are competitively added. Above pH 10.0 the addition reaction of iso-propylmercaptide ion occurs. From these rate equation, we found that observed data accorded satisifactorily with theoretical value over various pH range. Hence, the mechanism of nucleophilic addition reaction over wide pH range can be fully explained.