폐암환자 말초혈액의 자연살해세포 표현형(Natural Killer Cell Phenotypes)과 T임파구 아형 (T-Cell Subsets)의 의의

남재만,서지원,김남재,김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

The authors studied the NK cell phenotype and T cell subsets in 36 patients with bronchogenic carcinoma and 11 healthy controls. The results were summarized as follows. 1. The percentage of lymphocyte and total lymphocyte counts significantly decreased in cancer patients compared to normal control(p<0.05). 2. The OKT4^+(%) was significantly decreased in patients according to celltype (especially squamous cell cancer, adenocarcinoma and large cell carcinoma) and extended stage(stageⅢ) (p<0.05), but no change in limited stage(stage Ⅰ&Ⅱ). 3. The OKT8^+(%) and OKT4^+ ratio were not significantly changed in cancer patient. 4. According to cell type, there was no significant difference of NK cell phenotype between cancer patients and controls. 5. According to stage, there was no significant difference of NK cell phenotype between cancer patients and controls. 6. There was no significant difference of NK cell phenotype in cancer patients compared to normal controls. We concluded that NK cell phenotype had no significant difference between cancer patients and healthy controls. Further studies (including NK cell activity or other function tests of lymphocyte) are needed to analyze the roles of immunologic responses in patients with lung cancer.

호흡기감염증에 대한 Cefuroxime Axetil(Zinnat�)의 임상효과

김주옥,홍석철,김남재,서지원,김선영,노흥규 中央醫學社 1991 中央醫學 Vol.56 No.2

To evaluate the efficacy and safety of Zinnat in patients with respiratory tract infections, 20 patients were treated with Zinnat (250 mg b.i.d). Among 20 cases, 5 cases had pneumonia, 5 cases exacerbation of chronic bronchitis, 4 cases acute bronchitis, 3 cases bronchiectasis with infection, and acute tonsilitis, bronchial asthma with infection and emphysema with infection 1 case, respectively. The response was cure in 12 cases, partial response in 6 cases and no response in 2 cases. There were no significant clinical and laboratory side effects except dyspepsia and nausea (1 case) and diarrhea (1 case). Zinnat was considered to be useful agent against bacterial respiratory tract infections.

호흡기 감염증에 대한 Roxithromycin(Rulid^(�))의 임상 효과

노흥규,김남재,서지원,김주옥,김선영 최신의학사 1990 最新醫學 Vol.33 No.11

항결핵 6개월 단기요법의 성과 고찰

서지원,정연채,김남재,홍석철,김주옥,김선영,노흥규 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1

To evaluate the effect of 6-month short term antituberculosis chemotherapy with INH, Rifampin, Ethambutol, and Pyrazinamide(2HREZ/4HRE) in the patients with pulmonary and/or extrapulmonary tuberculosis, the authors prescribed 2HREZ/4HRE regimen in 79 tuberculosis patients for 6 months with measuring the sputum staining for AFB, chest X-ray findings, recurrence rates and possible side reactions of the treatment. The result were as follow; 1. Pulmonary Tuberculosis 1) Among the 56 pulmonary tuberculosis patients who had taken 2HREZ/4HRE regimen. 32 patients showed initial positivity in sputum AFB smear stain(57.2%). Negative conversion occurred usually within 2 months after initiation of chemotherapy and the mean period of negative conversion was 1.4 months. 2) Among the 56 pulmonary tuberculosis patients, chest X-ray finding changed in 41 patients (37.21%). From these 41 patients 39 patients showed continuous improvements in chest X-ray finding, though 3 patients showed initial aggravation in spite of continuous medication. The remainder 2 cases aggravated due to the failure of treatment. 3) Treatment failure occurred in 2 patients (3.57%) during the chemotherapy among 56 patients of pulmonary tuberculosis and they were infected with secondary drug-resistant strains of M. tuberculosis. 2. Extrapulmonary tuberculosis. Among 8 patients with tuberculous pleurisy and 15 patients with superficial tuberculous lymphadenitis, there were no evidence of treatment failure after completion of antituberculosis chemotherapy for 6 months with 2HREZ/4HRE regimen. 3. Follow-up study was performed from 6 months to 50 months after completion of antituberculosis chemotherapy and the relapse was not noted in both pulmonary and extrapulmonary tuberculosis patients group during this period. 4. Serum AST/ALT elevated in 9 patients(11.4%) during the treatment and this occurred usually within 3 months after the initiation of antituberculosis chemotherapy. However treatment interruption occurred in 2 patients (2.5%) due to the development of hepatitisone due to drug-induced hapatitis and the other due to type B viral hepatitis. In conclusion, we could find this 2HREZ/4HRE 6-months short-term antituberculosis regimen is effective and could be recommanded as a promising regimen for the treatment of tuberculosis.

FAS1 domain protein inhibits VEGF165-induced angiogenesis by targeting the interaction between VEGFR-2 and αvβ3 integrin.

Nam, Ju-Ock,Son, Hye-Nam,Jun, Eunsung,Cha, Kiweon,Lee, Byung-Heon,Park, Rang-Woon,Kim, In-San American Association for Cancer Research 2012 Molecular Cancer Research Vol.10 No.8

<P>It is known that VEGF receptors (VEGFR) and integrins interact with each other to regulate angiogenesis. We reported previously that the fasciclin 1 (FAS1) domain-containing protein, TGFBIp/beta ig-h3 (TGF-beta-induced protein) is an angiogenesis regulator that inhibits both endothelial cell migration and growth via alpha v beta 3 integrin. In an attempt to target the interaction between VEGFR-2 and alpha v beta 3 integrin, we determined whether the FAS1 domain region of TGFBIp/beta ig-h3 (FAS1 domain protein) can block the interaction between the two receptors, leading to the suppression of angiogenesis. In this study, we showed that FAS1 domain protein inhibits VEGF(165)-induced endothelial cell proliferation and migration via avb3 integrin, resulting in the inhibition of VEGF(165)-induced angiogenesis. We also defined a molecular mechanism by which FAS1 domain protein blocks the association between alpha v beta 3 integrin and VEGFR-2, showing that it binds to alpha v beta 3 integrin but not to VEGFR-2. Blocking the association of these major angiogenic receptors with FAS1 domain protein inhibits signaling pathways downstream of VEGFR-2. Collectively, our results indicate that FAS1 domain protein, in addition to its inhibitory effect on alpha v beta 3 integrin-mediated angiogenesis, also inhibits VEGF165-induced angiogenesis. Thus, FAS1 domain protein can be further developed into a potent anticancer drug that targets two principal angiogenic pathways. Mol Cancer Res; 10(8); 1010-20. (c) 2012 AACR.</P>

T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhancesanti-angiogenic and anti-tumor activity via αvβ3 and α5β1integrins

Ju-Ock Nam,Mi-Yeon Jung,Narendra Thapa,Byung-Heon Lee,Rang-Woon Park,In-San Kim 생화학분자생물학회 2008 Experimental and molecular medicine Vol.40 No.2

We made fusion protein of fastatin and FIII 9-10, termed simultaneously with αvβ3 and α5β1 integrins, both playing important roles in tumor angiogenesis. T-CAM can serve as a cel adhesion substrate mediating adhe-sion and migration of endothelial cells in αvβ3 and α5β1 integrin-dependent manner. T-CAM showed pro-nounced anti-angiogenic activities such as inhibition liferation, and induction of endothelial cell apoptosis. T-CAM also inhibited angiogenesis and tumor growth in mouse xenograft model. The anti-angiogenic and anti-tumoral activity of molecule like fastatin could be improved by fusing it with integrin-recognizing cell adhesion domain from other distinct proteins. The strategy of combining two distinct anti-angiogenic molecules or cell adhesion domains could facilitate designing improved anticancer agent of therapeutic value.

Cobalt chloride에 의해 유도되는 상피-중간엽 이행에서의 국소부착 단백질의 인산화의 역할 규명

Ju-Ock Nam(남주옥) 한국생명과학회 2011 생명과학회지 Vol.21 No.2

본 연구는 인간 폐암세포의 저산소 상태를 재현하기 위한 CoCl2의 처리 조건을 최적화 하였고, 최적화 된 저산소 상태에서 인간 폐암세포의 암화 과정 및 기전을 규명하였다. 인간 폐암세포, A549와 H460에 500 μM CoCl₂를 24시간 처리하였을 때 저산소 상태의 대표적인 전사인자, HIF-1α의 발현이 증가함을 확인하였고 인간 폐암세포들의 성장에는 전혀 영향을 미치지 않음을 확인하였다. 또한 CoCl₂를 처리한 인간 폐암 세포에서 상피-중간엽 이행(epithelial-to-mesenchymal-like transition)의 대표적인 마커인 E-cadherin 발현의 감소와 α-SMA의 증가를 확인하였고, 세포-세포 간 junction 부위가 깨어짐을 E-cadherin 형광염색 실험을 통하여 확인하였다. 더 나아가 CoCl₂를 처리한 인간 폐암 세포에서 상피-중간엽 이행의 분자적 기전을 밝히기 위해 세포벽에 존재하는 인테그린(integrin)의 발현을 웨스턴 블랏팅과 FACS분석을 통하여 알아본 결과, CoCl2를 처리한 인간 폐암세포에서 인테그린 β3발현의 증가를 확인하였다. 뿐만 아니라, CoCl₂를 처리한 인간 폐암세포에서 인테그린 β3의 하부 신호전달 물질인 국소부착 카이네이즈(FAK)의 인산화가 증가함을 확인하였다. 상기의 결과로서, 국소부착 카이네이즈의 인산화를 저해함으로써 인간 폐암세포가 악성세포로 전이되는 것을 저해할 수 있을 것으로 기대 되어진다. Hypoxia is a common condition found in a wide range of solid tumors and is often associated with metastasis and poor clinical outcomes. In the present study, we found that HIF-1α was induced by cobalt chloride (500 μM) treatment on human lung cancer cells, A549 and H460, for 24 hr. However, cobalt chloride (500 μM) did not affect cell proliferation of A549 and H460 in 48 hr. Cobalt chloride (500 μM) additionally induced epithelial-to-mesenchymal-like transition (EMT) such as reduced E-cadherin expression and increased α-SMA expression. These results were confirmed by immunofluorecence experiment in H460 cells. E-cadherin was localized on the outer cell membrane. However, when the cells were treated with 500 μM cobalt chloride for 24 hr, diffuse E-cadherin staining was observed, characteristic of a migratory mesenchymal phenotype. We also found that cobalt chloride induced integrin β3 expression and FAK phosphorylation in human lung cancer cells using western blotting and FACS anlaysis. Our data suggest that integrin β3-induced FAK phosphorylation may be developed into target molecules for blocking tumor metastasis.

PP-28 : Expression and Amplification of S. chibaensis J-59 xylA Gene in E. coli

(Ju Ock Nam),(Oh Seuk Lee),(Gun Young Heo),(Jae Ho Shin),(Jun Ho Choi),(In Koo Rhee) 한국응용생명화학회(구 한국농화학회) 1999 한국응용생명화학회 학술발표회 Vol.1999 No.1

Poster Session 2 : Immunology Tumor Biology ; Regulation of tumor angiogenesis by fastatin, the 4th FAS1 Domain of βig-h3 through αvβ3 integrin

( Ju Ock Nam ),( Ha Won Jeong ),( In San Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2004 생화학분자생물학회 춘계학술발표논문집 Vol.2004 No.-

玄蔘의 Saponin 成分 檢索 : on the Saponin of the Radix

남인숙,배병숙,최보향,최인수,최태수,김동언,김언주,정미영,조규옥 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

Crude saponin(100g) were obtained by extracting the radix(5kg) of Scrophalariae koraiensis Nakai. Crude saponin were positive in the Liebermann-Burchard test. We observed 5 sports by TLC using BuOH saturated with H_2O : ethyl acetate : water(4:1:5, upper phase) as solvent and 1% Ce(SO_4)_2 in 10% H_2SO_4 as a color former. We divided into 3 fractions by common column chromatography using BuOH saturated with H_2O : ethylacetate : water(4:1:5, upper phase) as solvent.