Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

응급실에 내원한 환자에서 어지럼증의 원인과 임상양상의 분석

문원식,정경운,위준선,문정미,전병조,김용권,소정일,류진호,허탁,민용일 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Background: Recently, The number of patients who have been complaining of the vertigo or dizziness has been increasing due to rapid growth of elderly population and senile disease. The differentiation of dizzy patients is not familiar to most emergency physicians. This study was designed to differentiate true vertigo and to investigate the clinical difference among central vertigo, peripheral vertigo, and other causes of dizziness. Methods: The authors analyzed the cases of 237 dizzy patients who visited the emergency department of Chonnam University Hospital during the recent 2 years. For the base of dizziness, associated past illnesses, severity, nystagmus type, and the causes of central and peripheral vertigo. Results: Female patients were 142(59.9%) and male patients were 95(40.1%). The most common age group was the 6th decade. The origin of dizziness was classified as peripheral cause(25.3%), central cause(32.9%), and others(41.8%). As to nature of the dizziness, the rotatory sense was dominant in peripheral vertigo and the floating sense and blurred vision in central vertigo. Peripheral vertigo was triggered by position change of head and body, and central vertigo by the eye movement. Audiograms I showed that most patients with central vertigo had normal hearing, but 46.7% of thoswith peripheral vertigo had an abnormal hearing disturbance. Nystagmus was more prevalent in peripheral vertigo Conclusion: History taking and physical examination played an important role in the diagnosis of dizzy patients. An important part of the diagnosis of a dizzy patient could be to evaluate the peripheral origin, the central origin and others causes. Because central vertigo may be associated with a significant neurological pathology, spectral radiological studies, including MRI and CT, are mandatory to rule out a devastating brain lesion.

Bromelain과 Zn-Methlonnine 혼합 급여가 젖소의 산유량 및 체세포수에 미치는 영향

정유진,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

Holstein cows(n=8) were assigned to control diet(n=4) and treatment diet(n=4) containing products of Bromelain(50g/kg) and Zn-methionine (133g/kg). Basal diet was mixed as total mixed rations with 60% concentrate and 40% roughage(rice straw) and fed for 8 weeks. The milk production, somatic cell counts in milk were measured and determined. The results were summarized as follow. Average milk production was higher for cows fed treatment diet(30.2kg/d) than cows fed control diet(29.6kg/d) (P<0.05). The somatic cell counts was significantly lower for cows fed treatment diet(179.8×10³/ml) than cows fed control diet (260.8x10³/ml)(P<0.05). In conclusion, supplementation of both Bromelain and Zn-methionine increased milk production and reduced somatic cell counts in milk. 본 실험은 사료 첨가제로써 Bromelain과 Zn-methionine의 급여가 착유우의 산유량 및 체세포 수 함량에 미치는 영향을 규명하고자 실시하였다. 홀스타인 착유우 8두(대조구 4두 및 처리구 4두)를 공시하여 대조구는 기본 사료를 급여하고 처리구에서는 Zn-methionine 133g/kg(Zn 20 및 methionine 46.7g)과 bromelain 50g/kg(br 30g/kg)을 경구투여하였다. 그리고 시험은 착유우의 비유초기에 8주간 실시하였다. 본 시험에서 얻은 결과는 다음과 같다. 두당 평균 유량은 대조구(29.6kg)에 비하여 처리구(30.2kg)에서 높게 나타났다(P<0.05). 우유내 체세포수는 대조구(260.8Xl0³ml)에 비하여 처리구(179.8X10³ml)에서 현저하게 낮게 나타났다(P<0.05). 따라서 이들 첨가물의 급여는 착유우의 산유량을 증가시키고 우유의 위생상 지표가 될 수 있는 체세포수의 감소를 가져오는 결과를 얻었다.

반응표면분석에 의한 단감식초 제조조건의 모니터링

정용진,서권일,이기동,윤광섭,강미정,김광수 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.1

To utilize deteriorated sweet persimmon effectively, response surface methodology(RSM) was used to determine the optimal vinegar fermentation conditions and monitored by a divided two stage fermentation. The optimum conditions for maximum alcohol content were obtained when the first stage (alcohol fermentation) was carried out with an initial sugar concentration of 18.5°Brix, agitation rate of 140.8 rpm, fermentation time of 127.6 hr. When sugar concentration was 14°Brix, maximum alcohol content(7.1%) was predicted at fermentation conditions of 160 rpm in agitation rate, 140 hr in fermentation time. The optimum conditions for maximum acidity were obtained when second stage(vinegar fermentation) was carried out 249.5 rpm in agitation rate, 148.8 hr in fermentation time. Predicted values at the optimum conditions were similar to experimental values.

保險事故發生前의 保險給付請求權의 讓渡

鄭鎭玉,鄭容相 釜山 外國語 大學校 1998 外大論叢 Vol.18 No.2

Die vorliegende Arbeit befaβt sich mit den interessenkonflikten, die sich dann ergeben konnen, wenn der Versicherungsnehmer Rechte und Asnpruche aus einem Lebensversicherungsvertrag auf einen Dritten Ubertragt im Rahmen einer Sicherungszession oder Vollzession. Es soll hierbei nicht nur untersucht werden, weiche Voraussetzungen fur eine wirksame Abtretung vorliegen mussen, sondern vielmehr auch die Rechtsposition der Beteiligten nach erfolgter Abtretung dargestellt werden. Im Rahmen der jeweiligen Interessenkonflikte wird die Rechtslage bei der Versicherung mit unwiderruflicher und widerruflicher Begunstigung dargelegt. Der Versicherungsnehmer kann einen zu seinen Lebzeiten auftretenden Geldbedarf durch Ruckgriff auf den Zeitwert seiner Lebensversicherung nur befriedigen, wenn er sich deren Spar-bzw. Kreditsicherungsfunktion zunutze machen kann. Die Vorschrift des §731Abs. 2 Koreanischen Handelsgesetz setzt voraus. daβer imstande ist, die vertraglichen Gestaltungsrechte auszuuben bzw. uber die geldwerten Versicherungsanspruche durch Abtretung frei zu verfugen. Diesem interesse des Versicherungsnehmers an freier Negoziabilitat widerstreitet das interesse des Begunstigten, bei Eintritt des Versicherungdfalls in den Genuβder versicherungssumme zu gelangen. Die Durchsetzung dieses Versorgungsinteresses erfordert eine durch Abtretung des Versicherungsnechmers nicht zu beeintrachtigende Rechtsposition des Dritten. Im Arbeit wird erortert, ob und inwieweit die Rechtsposition des Begunstigten durch die Abtretung von Rechte und Anspruche aus einem Lebensversicherungsvertrag eingeschrankt wird. In Hinblick auf die Frage, welche Voraussetzungen fur eine wirksame Abtretung vorliegen mussen, eine Abtretung der Versicherungsanspuche ist dem versicherer gegenuber nur erst dann wirksam, wenn sie der Verfugungsberechtigte angezeigt hat. Die Rechtsfolgen einer Anzeige der Abtretung regelt die Vorschrift des §450 Koreanischen BGB. Keine Ubergabe des Versicherungsscheines verlangt fur die Wirksamkeit der Abtretung. Fur die Frage, wie die Abtretung des Versicherungsanspruches druch den Versicherungsnehmer auf die Rechtsstellung auswirkt, ist zwischen Vollzession und Sicherungsabvtretung, zu unterscheiden. Bei der Vollzession im Gegensatz zur Sicherungszession soll dem zessionar nach dem Willen der Parteien die eingeschankte Glaubigerstellung hinsichtlich der Versicherungsleistung zugewendet werden, weil die dem Versicherer anzuzeigende Abtretung regelmaβig den endgunstigung beinhaltet. Nach meiner Meinung geht dergegenuber davon aus, auch die Sicherungszession einen widerruf der Bezugsberechtigung bedeutet und daβdieser Widerruf mit Anzeige bei dem Versicherer wirksam wird, ohne daβ insoweit eine ausdruckliche Erklarung uber den Widerruf der Bezugsberechtigung abzugeben ist. Eine ganz andrere und noch weitergehende Frage ist es, ob die Sicherungszession einen endgultigen Widrruf der Bezugsberechtigung enthalt oder ob der Widerruf nur fur die Dauer der Abtretung wieder auflebt. Anders als bei der Vollzession ist mit der Sicherungszession regelmabig kein endgultige Widerruf der Bezugsberechtigung verbunden, vielmehr ist davon auszugehen, dab nach erfolgter Ruckabtretung das ursprunglich Bezugsrecht wieder auflebt. Sofern der Versicherungsnehmer fur die Leistung im Erlebens oder im Todefall unwiderruflich einen Bezugsberechtigten bezeichnet hat, erwirbt dieser bereits mit der Benennung ohne weiteres Zutun den Anspruch gegen den Versicherer.

흰쥐에서 말초신경 손상후 뒤뿌리신경절세포의 신경영양인자와 그 수용체의 발현 양상

백선용,양만석,문총석,김봉선,김재봉,김진정 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.2

2단계 발효에 의한 포도식초와 재래식 포도식초의 품질 비교

정용진,이명희,서권일,김주남,이용수 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.4

Grape vinegar(A) and grape onion vinegar(B) added (3% of onion juice) were produced through two stages of fermentation(alcohol fermentation and acetic acid fermentation) to increase the grape's use. Grape wine which contained 5.6% alcohol was produced on the 3rd day of first stage. Then through the second stages, grape vinegar, of which total acidity was 5.37% was produced. The quality of (A) and (B) which was produced through the two stages of fermentation was compared with the traditional grape vinegar(C,D) in the market. The content of sugar in (D) was a little higher such as 5.4 °Brix than others. That of (A) and (B) was 5.13, 4.98 °Brix respectively. The content of remaining alcohol in (C) was high such as 0.23% comparatively, But there was no remaining alcohol in (A) and (B). The content of acetic acid was 4.3~5.3% as a major organic acid of vinegars. The content of tartaric acid was 340.0 in (A), 316.7 in (B), 322.6 in (C) and 391.7㎎% in (D). The content of lactic acid was distinctly high such as 277.4㎎% in (D). There were differences such as 9.2~15.5㎎% in the content of total free amino acids among grape vinegars. (D) contained 15.5㎎% of total free amino acid and (B) also highly such as 12.0㎎%. Potassium was high in grape vinegars. The content of potassium, sodium and copper was higher in (A) than (B).

메밀묵의 제조조건에 따른 텍스쳐 및 관능적 특성 변화

정용진,이명희,서지형,이기동 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.2

Response surface methodology (RSM) was used for optimizing preparation conditions and monitoring the quality of buckwheat mook prepared using buckwheat starch. The textures(hardness, adhesiveness cohesiveness and gumminess) of buckwheat mook were decreased in inverse proportion to the increase of water content. The L and b values of Hunter color parameters in buckwheat mook were increased in proportion to the increase of water content. However, The a value of Hunter color parameters of buckwheat mook were decreased in inverse proportion to the increase of water content. Organoleptic properties(color, form, taste and mouth-feel) of buckwheat mook showed a maximum score in 700㎖(water content), 14min(gelatinization time).

감과실 알코올 발효를 위한 효모의 분리

정용진,서권일,신승렬,서지형,강미정,김광수 동아시아식생활학회 1997 동아시아식생활학회지 Vol.7 No.4

To utilize abundant poor persimmon fruits effectively, it was studied on alcohol fermentation strains which were isolated and indentified from poor persimmon fruits. The strains which had exellent alcohol production ability were analysized morphogical, cultural characteristics and identified Saccharomyces cerevisiae YJK 20, Sacahroimyces kluveri DJ 97. Alcohol production ability between the isolated strains and industrial alcohol yeasts was compared in the YPD medium, persimmon extract medium. Isolated strains had high alcohol production ability in both of two medium, respectively. So it was expected that the strains, YKJ 20 and DJ 97 had good alcohol fermentation ability of persimmon fruits.