Experimental study on air-water countercurrent flow limitation in a vertical tube based on measurement of film thickness behavior

Wan, Jie,Sun, Wan,Deng, Jian,Pan, Liang-ming,Ding, Shu-hua Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.6

The gas-liquid counter-current flow limitation (CCFL) is closely related to efficient and safety operation of many equipment in industrial cycle. Air-water countercurrent flow experiments were performed in a tube with diameter of 25 mm to understand the triggering mechanism of CCFL. A parallel electrode probe was utilized to measure film thickness whereby the time domain and frequency domain characteristics of liquid film was obtained. The amplitude of the interface wave is small at low liquid flow rate while it becomes large at high liquid flow rate after being disturbed by the airflow. The spectral characteristic curve shows a peak-shaped distribution. The crest exists between 0 and 10 Hz and the amplitude decreases with the frequency increase. The analysis of visual observation and characteristic of film thickness indicate that two flooding mechanisms were identified at low and high liquid flow rate, respectively. At low liquid flow rate, the interfacial waves upward propagation is responsible for the formation of CCFL onset. While flooding at high liquid flow rate takes place as a direct consequence of the liquid bridging in tube due to the turbulent flow pattern. Moreover, it is believed that there is a transition region between the low and high liquid flow rate.

Acetoaminophen-induced accumulation of 8-oxodeoxyguanosine through reduction of Ogg1 DNA repair enzyme in C6 glioma cells

Jie Wan,배명애,송병준 생화학분자생물학회 2004 Experimental and molecular medicine Vol.36 No.1

Large doses of acetaminophen (APAP) could cause oxidative stress and tissue damage through production of reactive oxygen/nitrogen (ROS/RNS) though ROS/RNS are known to modify DNA, the efect of APAP on DNA modifications has not ben studied systematically. In this study, we investi-gate whether large doses of APAP can modify the nuclear DNA in C6 glioma cels used as a model system, because these cells contain cytochrome P450-related enzymes responsible for APAP me-tabolism and subsequent toxicity (Geng and Stro-bel, 1995). Our results revealed that APAP pro-duced ROS and significantly elevated the 8-oxo- C6 glioma cells in a time and concentration depen-dent maner. APAP significantly reduced the 8- oxodG incision activity in the nucleus by de-creasing the activity and content of a DNA repair enzyme, Og1. These results indicate that APAP in large doses can increase the 8-oxodG level partly through significant reduction of Ogg1 DNA repair enzyme.

Predicting Non Performing Loan of Business Bank with Data Mining Techniques

Wan Jie,Yue Zeng-lei,Yang Dong-hui,ZhangYu,Liu Jiao,Liu Zhi,Liu Jinfu 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.12

The non-performing loans (NPL) prediction plays an important role in business bank. However, there is still a large gap between the requirement of prediction performance and current techniques. In this paper data mining approaches is used to predict the NPL. Both macroeconomic and bank-specific variables are collected to form the feature set firstly. Based on selected features, the study firstly applies single basic classifiers such as decision tree, k nearest neighbors and support vector machine (SVM) to model the problem of NPL. Bagging and AdaBoost are described in this paper as two different method of multiple classifier fusion, to build prediction models. In this experiment, non-performing loans data with 96 features and 10415 instances of a business bank is collected. F-mean and The Area under the ROC Curve (AUC) are considered as metrics of classification. The results illustrate that multiple classifier fusion algorithms outperform single basic classifier. The model built by multiple classifiers fusion can produce better prediction results. Furthermore, the AdaBoost method performs much better than bagging method in processing NPL.

A Critical Role of OsMADS1 in the Development of the Body of the Palea in Rice

Jianmin Wan,Wenwei Zhang,Jie Zhang,Yue Cai,Haigang Yan,Jie Jin,Xiaoman You,Liang Wang,Fei Kong,Ming Zheng,Guoxiang Wang,Ling Jiang 한국식물학회 2018 Journal of Plant Biology Vol.61 No.1

OsMADS1 plays crucial roles in the specification of floral organ identity and the determinacy of floral meristem in rice (Oryza sativa). Here we isolated a rice mutant cy15 with defective spikelets. Map-based cloning identified cy15 as a novel mutation in the C terminus of OsMADS1. Severe cy15 spikelets contained normal inner whorl organs but defective paleas, opposite to four abnormal whorls of Osmads1 flowers reported previously. In the cy15 spikelets, the size of the body was remarkably reduced in paleas, indicating a critical role of OsMADS1 in its differentiation. Moreover, the development of palea was impaired in the early stage of the cy15 spikelets. The sub-subcellular location of the mutant OsMADS1 was also affected in cy15. On the other hand, the truncated C terminus of the mutant OsMADS1 from cy15 still showed a significant transcriptional activator function. Meanwhile, though the mutant OsMADS1 retained intact M, I and K domains, its interactions with two highly homologous A-class proteins, OsMADS15 or OsMADS14, were weakened or abolished. Our data suggested that the C-terminal sequence of OsMADS1 might be required for maintaining normal morphology of spikelets in rice.

The Influence of South Korea’s OFDI under the Effects of Multinational Enterprises’ Investment Motivations and Host Country Institutions

Jie Gao,Wanli Liu,Jianlin Li,Ke Yuan 한국무역학회 2022 Journal of Korea trade Vol.26 No.5

Purpose – This study aims to analyze the influence of South Korea’s outward foreign direct investment (OFDI) under the effect of both multinational enterprises’ (MNEs) investment motivation and host country institutions. Some suggestions are put forward with regard to South Korean MNEs participating in and integrating into the fierce and changeable world of international market competition. Design/methodology – The basic hypotheses are that MNEs’ investment motivations and the host country’s superior institutions both boost South Korea’s OFDI in those host countries. South Korea’s OFDI is divided into investment choice stage and investment scale stage. A Heckman two-stage selection model is established for empirical analysis, using the panel data of South Korea’s OFDI and related variables, from 2002 to 2019. Findings – (1) The influence on the investment scale of South Korea’s OFDI is more regular and noteworthy than the influence on investment choice. (2) In the investment scale stage, there are obvious motivations to seek markets, labor force and superior technology, but not natural resources. (3) In the investment scale stage, the South Korea’s OFDI is more obviously attracted by the host country’s superior political institutions, economic institutions and legal institutions, but not cultural institutions. Originality/value – The choices of variables and uses of model expand the theoretical basis and empirical method of OFDI research. The results of the empirical study also provide some reference for the transnational investment of South Korean MNEs and the investment policy formulation of the South Korean government.

Analysis of the Genome Sequence of Strain GiC-126 of Gloeostereum incarnatum with Genetic Linkage Map

( Wan-zhu Jiang ),( Fang-jie Yao ),( Ming Fang ),( Li-xin Lu ),( You-min Zhang ),( Peng Wang ),( Jing-jing Meng ),( Jia Lu ),( Xiao-xu Ma ),( Qi He ),( Kai-sheng Shao ),( Asif Ali Khan ),( Yun-hui Wei 한국균학회 2021 Mycobiology Vol.49 No.4

Gloeostereum incarnatum has edible and medicinal value and was first cultivated and domesticated in China. We sequenced the G. incarnatum monokaryotic strain GiC-126 on an Illumina HiSeq X Ten system and obtained a 34.52-Mb genome assembly sequence that encoded 16,895 predicted genes. We combined the GiC-126 genome with the published genome of G. incarnatum strain CCMJ2665 to construct a genetic linkage map (GiC-126 genome) that had 10 linkage groups (LGs), and the 15 assembly sequences of CCMJ2665 were integrated into 8 LGs. We identified 1912 simple sequence repeat (SSR) loci and detected 700 genes containing 768 SSRs in the genome; 65 and 100 of them were annotated with gene ontology (GO) terms and KEGG pathways, respectively. Carbohydrate-active enzymes (CAZymes) were identified in 20 fungal genomes and annotated; among them, 144 CAZymes were annotated in the GiC-126 genome. The A mating-type locus (MAT-A) of G. incarnatum was located on scaffold885 at 38.9 cM of LG1 and was flanked by two homeodomain (HD1) genes, mip and beta-fg. Fourteen segregation distortion markers were detected in the genetic linkage map, all of which were skewed toward the parent GiC-126. They formed three segregation distortion regions (SDR1-SDR3), and 22 predictive genes were found in scaffold1920 where three segregation distortion markers were located in SDR1. In this study, we corrected and updated the genomic information of G. incarnatum. Our results will provide a theoretical basis for fine gene mapping, functional gene cloning, and genetic breeding the follow-up of G. incarnatum.

Constructing Hierarchical Porous Bacterial Cellulose Outer Layer for Enhanced SMCs Infiltration in a Double-Layered Vascular Graft

Jie Wang,Weijuan Nie,Ming Wang,Chen Zhang,Jian Li,Honglin Luo,Quanchao Zhang,Zhiwei Yang,Yizao Wan 한국섬유공학회 2023 Fibers and polymers Vol.24 No.11

Natural blood vessels possess a multi-layered structure. Herein, we present a bio-inspired double-layered integrated tubular graft with a compact inner layer and a porous outer layer. The inner layer consists of nanofibrous bacterial cellulose (BC) and microfibrous cellulose acetate (CA), which favors the adhesion and proliferation of endothelial cells (ECs). The outer layer, with hierarchical pores including nano-sized pores from pristine BC, median-sized pores (dozens of microns) created by particle leaching, and a large pore (hundreds of microns) array created by laser ablation, is appropriate for the penetration of vascular smooth muscle cells (SMCs). The in-situ biosynthesis was employed to "suture" the two layers, aiming to achieve an integrated tubular structure. The morphology, mechanical properties, and cell behavior of the tubular grafts were characterized. The in vitro study demonstrates that the unique outer layer possesses enhanced SMC penetration compared to the counterpart without hierarchical pores. Overall, the findings of this study demonstrate the potential of such a biomimetic integrated vascular graft for vascular replacement and regeneration.

Structural Conservation and Food Habit-related Liver Expression of Uncoupling Protein 2 Gene in Five Major Chinese Carps

( Wan Qin Liao ),( Xu Fang Liang ),( Lin Wang ),( Ling Fang ),( Xiao Tao Lin ),( Jun Jie Bai ),( Qing Jian ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.4

Prognostic Value of Serum Epstein–Barr Virus Antibodies and Their Correlation with TNM Classification in Patients with Locoregionally Advanced Nasopharyngeal Carcinoma

Wan-Ru Zhang,Yu-Yun Du,Chun-Yan Guo,Han-Xing Zhou,Jie-Yi Lin,Xiao-Han Meng,Hao-Yuan Mo,Dong-Hua Luo 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose This study assessed the correlation between Epstein-Barr virus (EBV) biomarkers and the eighth American Joint Committee on Cancer staging system and the prognostic values of IgG antibodies against replication and transcription activator (Rta-IgG), IgA antibodies against Epstein-Barr nuclear antigen 1, and BamH1 Z transactivator (Zta-IgA) in locoregionally advanced nasopharyngeal carcinoma (NPC) patients. Materials and Methods Serum EBV antibody levels were measured by enzyme-linked immunosorbent assay in 435 newly diagnosed stage III-IVA NPC patients administered intensity-modulated radiation therapy±chemotherapy. The primary endpoint was progression-free survival (PFS). Results Rta-IgG and Zta-IgA levels were positively correlated with the N category and clinical stage. Patients with high Rta-IgG levels (> 29.07 U/mL) showed a significantly inferior prognosis as indicated by PFS (77% vs. 89.8%, p=0.004), distant metastasis–free survival (DMFS) (88.3% vs. 95.8%, p=0.021), and local recurrence-free survival (LRFS) (91.2% vs. 98.3%, p=0.009). High Rta-IgG levels were also significantly associated with inferior PFS and LRFS in multivariable analyses. In the low-level EBV DNA group (≤ 1,500 copies/mL), patients with high Rta-IgG levels had significantly inferior PFS and DMFS (both p < 0.05). However, in the high-level EBV DNA group, Rta-IgG levels were not significantly associated with PFS, DMFS, and LRFS. In the advanced T category (T3-4) subgroup, high Rta-IgG levels were also significantly associated with inferior PFS, DMFS, and LRFS (both p < 0.05). Conclusion Rta-IgG and Zta-IgA levels were strongly correlated with the TNM classification. Rta-IgG level was a negative prognostic factor in locoregionally advanced NPC patients, especially those with advanced T category or low EBV DNA level.

Associations Between TLR9 Polymorphisms and Cancer Risk: Evidence from an Updated Meta-analysis of 25,685 Subjects

Wan, Guo-Xing,Cao, Yu-Wen,Li, Wen-Qin,Li, Yu-Cong,Zhang, Wen-Jie,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

A meta-analysis incorporating 34 case-control studies from 19 articles involving 12,197 cases and 13,488 controls was conducted to assess the effects of three genetic variants of Toll-like receptor 9 (TLR9): rs187084, rs352140, and rs5743836. Studies on associations between TLR9 polymorphisms and cancer risk were systematically searched in electronic databases. The reported odds ratios (OR) and 95% confidence intervals (CI) were pooled to assess the strength of any associations. The results showed that the rs187084 polymorphism was significantly associated with an increased risk of cancer (CC vs TC+TT: OR=1.14, 95% CI=1.02-1.28), specifically cervical cancer (C vs T: OR=1.19, 95% CI=1.05-1.34; TC vs TT: OR=1.32, 95% CI=1.10-1.58; CC vs TT: OR=1.31, 95% CI=1.03-1.68; CC+TC vs TT: OR=1.32, 95% CI=1.11-1.56), and that this association was significantly positive in Caucasians (CC vs. TC+TT: OR=1.18, 95% CI=1.01-1.38). The rs352140 polymorphism had a protective effect on breast cancer (GA vs GG: OR=0.77, 95% CI=0.66-0.89), whereas the rs5743836 polymorphism was likely protective for digestive system cancers (CC+TC vs TT: OR=0.81, 95% CI=0.66-0.98). In conclusion, our results suggest that the rs187084 polymorphism may be associated with an elevated cancer risk, whereas polymorphisms of rs352140 and rs5743836 may play protective roles in the development of breast and digestive system cancers, respectively. From the results of this meta-analysis further large-scale case-control studies are warranted to verify associations between TLR9 polymorphisms and cancer.