Variable Research on Engineering Characteristics of Soybean Urease Reinforced Sand

Meng Cui,Huihui Xiong,Jun-jie Zheng,Suying Lv,Mingjuan Cui,Xiao Fu,Shangyu Han 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8

In this study, based on soybean urease and standard sand, the unconfined compressive strength test and permeability test of four variables of urease concentration, urea concentration, calcium chloride concentration and sodium alginate content under different combinations of values were carried out, and the range analysis and variance analysis of test data were carried out. On this basis, the coupling model of unconfined compressive strength and permeability coefficient of soybean urease reinforced sand was established based on four test variables. It was found that the engineering characteristics of soybean urease reinforced sand were significantly affected by the combination of variable values. The maximum value of unconfined compressive strength under the test combination condition was 6.5 times of the minimum value, and the minimum value of permeability coefficient was only 1/4 of the maximum value. The primary and secondary order of influence variables of unconfined compressive strength was: urea concentration > calcium chloride concentration > urease concentration > sodium alginate content, and the primary and secondary order of influence variables of permeability coefficient was: urea concentration > calcium chloride concentration > sodium alginate content > urease concentration. The influence of urea concentration on unconfined compressive strength and permeability coefficient was obvious, and the influence of the other three variables was not obvious. In addition, it was confirmed that the established coupling model of unconfined compressive strength and permeability coefficient of soybean urease reinforced sand had high correlation coefficient and significant linear regression, which can be used for subsequent research and engineering application.

Bacterial and Fungal Diversity in the Starter Production Process of Fen Liquor, a Traditional Chinese Liquor

Xiao-Ran Li,En-Bo Ma,Liang-Zhen Yan,Han Meng,Xiao-Wei Du,Zhe-Xue Quan 한국미생물학회 2013 The journal of microbiology Vol.51 No.4

Fermented foods and beverages are important parts of human diet. Fen liquor, a Chinese liquor is a fermented beverage that uses a traditional fermentation process. Starters are the main microbial source and also provide nutrients for microorganisms during fermentation. In this study, starters of Fen liquor were produced through a complex traditional fermentation process. To investigate the community structure and the composition of microorganisms in the starter production process, bacterial 16S rRNA and fungal internal transcribed spacer (ITS) regions were sequenced using clone libraries and pyrosequencing, respectively. There was much higher diversity among the bacteria than among the fungi in the starter production process. Bacteria on the surface of the starters belonged mostly to the Lactobacillaceae family,while members of the Bacillacae family were dominant in the interior of the samples that lacked access to air and water. In the fungi population, diversity was high only in the raw material. In all other samples, nearly all of the fungal sequences were from Pichia kudriavzevii, a member of the Saccharomycetaceae family. Nearly all samples showed similar fungal community structures, indicating that there was little change in the fungal community. To the best of our knowledge, this is the first report to reveal the whole process of the starter production of Chinese traditional liquor. The findings obtained in this study provide new insights into understanding the composition of the microbial community during the traditional Chinese liquor starter production process and information about the production process control and monitoring.

Lack of Effects of HER-2/neu on Prognosis in Colorectal Cancer: a Meta-analysis

Han, Jun,Meng, Qing-Yang,Liu, Xiao,Xi, Qiu-Lei,Zhuang, Qiu-Lin,Wu, Guo-Hao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: The prognostic value of human epidermal growth factor receptor-2 (HER-2/neu) for survival of patients with colorectal cancer (CRC) is still ambiguous. We therefore performed a meta-analysis to evaluate its prognostic significance. Materials and Methods: We searched the MEDLINE and EMBASE databases for published literature investigating associations between HER-2/neu status and overall survival of patients with CRC. A meta-analysis was performed using a DerSimonian-Laird model and publication bias was investigated by Begg's and Egger's tests. Subgroup analysis was also conducted according to the study design type, study quality score, cut-off value for HER-2/neu overexpression, publication region, patient number and publication year. Results: A total of 17 eligible studies involving 2,347 patients were identified for this meta-analysis. The combined hazard ratio (HR) was 1.31 (95% confidence interval (CI): 0.96-1.79), suggesting that HER-2/neu overexpression was not significantly associated with overall survival of patients with CRC. However, subgroup analysis revealed that HER-2/neu overexpression had an unfavorable impact on survival when the analysis was restricted to subgroups of study quality score ${\leq}5 $(HR=1.56, 95%CI: 1.17-2.10), Asian patients (HR=1.74, 95%CI: 1.22-2.49), patient number ${\leq}106$ (HR=1.57, 95%CI: 1.01-2.44), publication year before 2003 (HR=1.59, 95%CI: 1.02-2.49), and prospectively designed study (HR=3.62, 95%CI: 1.42-9.24). The effect disappeared in subgroups of study quality scores > 5 (HR=0.69, 95%CI: 0.33-1.44), non Asian patients (HR=1.14, 95%CI: 0.77-1.70), patients' number > 106 (HR=1.07, 95%CI: 0.67-1.72), publication year after 2003 (HR=1.13, 95%CI: 0.76-1.69), and retrospectively designed study (HR=1.22, 95%CI: 0.89-1.67). Conclusions: Our meta-analysis suggests that HER-2/neu overexpression might not be a significantly prognostic indicator for patients with CRC. Further studies are required to confirm these results.

A Highly Active Alpha Amylase from Bacillus Licheniformis: Directed Evolution, Enzyme Characterization and Structural Analysis

( Yi Han Liu ),( Shu Ai Fan ),( Xiao Guang Liu ),( Zhi Meng Zhang ),( Jian Ling Wang ),( Zheng Xiang Wang ),( Fu Ping Lu ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.7

The stability of Bacillus licheniformis alpha-amylase (BLA) under acid condition was enhanced through direct evolution using the error-prone polymerase chain reaction. One beneficial mutation site, H281I, was obtained in BLA. The specific activity of H281I was 161/352 U/mg, which was 62.6/27.5% higher than that of the wild-type (WT) (99/276 U/mg) at pH 4.5/6.5 and 95°C. The pH optimum for H281I was decreased about 1 unit, whereas no significant changes of optimum temperature and thermostability were observed compared with the wild type (WT). The kcat/Km value of H281I was 1.7-/1.4-fold higher at pH 4.5/6.5, respectively, than that of WT. The structure model analysis indicated that the H281I mutation altered the predicted interaction between the amino acid residues at 281 and 273, thus creating a conducive local environment for substrate binding, as reflected by its decreased Km, and consequently increased the specific activity.

Kinetics and computational docking studies on the inhibition of tyrosinase induced by oxymatrine.

Liu, Xiao-Xia,Sun, Shi-Qing,Wang, Yu-Jie,Xu, Wei,Wang, Yi-Fang,Park, Daeui,Zhou, Hai-Meng,Han, Hong-Yan Humana Press 2013 Applied biochemistry and biotechnology Vol.169 No.1

<P>A combination of enzymatic inhibition kinetics and computational prediction was employed to search for an effective inhibitor of tyrosinase. We found that oxymatrine significantly inhibited tyrosinase, and that this reaction was not accompanied by detectable conformational changes. Kinetic analysis showed that oxymatrine reversibly inhibited tyrosinase in a mixed-type manner. Measurements of intrinsic and ANS-binding fluorescences showed that oxymatrine did not induce any conspicuous changes in the tertiary structure. We also conducted a docking simulation between tyrosinase and oxymatrine using two docking programs, Dock6.3 and AutoDock4.2 (binding energy was -118.81 kcal/mol for Dock6 and -8.04 kcal/mol for AutoDock4). The results also suggested that oxymatrine interacts mostly with the residues of CYS83 and HIS263 in the active site of tyrosinase. This strategy of predicting tyrosinase inhibition by simulation of docking coupling with kinetics may prove useful in screening for potential tyrosinase inhibitors. Knowledge of tyrosinase inhibition can provide medical, cosmetic, and agricultural applications. Our study suggests that oxymatrine is an important agent for various applications related to pigment formation.</P>

Anti-HIV-1 Activity of Lignans from the Fruits of Schisandra rubriflora

Wei-Lie Xiao,Rui-Rui Wang,Wei Zhao,Ren-Rong Tian,Shan-Zhai Shang,Liu-Meng Yang,Jian-Hong Yang,Jian-Xin Pu,Yong-Tang Zheng,Han-Dong Sun 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.5

This study investigated the 70% aqueous acetone extract of the fruits of Schisandra rubriflora which led to the isolation of eight lignans, including a new isolate, rubrisandrin C (1), and seven known lignans (2-8) . The structure of 1 was established by extensive 1D and 2D NMR spectroscopy and its absolute stereochemistry was determined by CD spectrum. Compounds 1-5 and 7-8 were evaluated for their anti-HIV-1 activity that showed inhibitory activity on HIV-1IIIB induced syncytium formation with EC50 values in the range of 2.26~20.4 μg/mL. Compounds 1 and 7 exerted their obvious protection of HIV-1IIIB inducted MT-4 host cells lytic effects with a selectivity index of 15.4 and 24.6, respectively.

Thalidomide Combined with Chemotherapy in Treating Patients with Advanced Colorectal Cancer

Huang, Xin-En,Yan, Xiao-Chun,Wang, Lin,Ji, Zhu-Qing,Li, Li,Liu, Meng-Yan,Qian, Ting,Shen, Hui-Ling,Gu, Han-Gang,Liu, Yong,Gu, Ming,Deng, Li-Chun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17

Objective: To assess the safety and effectiveness of thalidomide (produced by CHANGZHOU PHARMACEUTICAL FACTORY CO.LTD) combined with chemotherapy in treating patients with advanced colorectal cancer. Method: A consecutive cohort of pretreated patients with advanced colorectal cancer were treated with thalidomide combined with chemotherapy. And chemotherapy for patients with advanced colorectal cancer were administered according to the condition of patients. Thalidomide was orally administered at a dosage of 50mg/day to 150mg/day before sleeping for at least 14 days. After at least 14 days of treatment, safety and side effects were evaluated. Results: There were 12 female and 3 male patients with advanced cancer recruited into this study, including 9 patients with colon, 6 patients with rectal cancer. The median age of patients was 57(41-82) years. Partial response was observed in 2 patients (2/15), and stable disease in 3 patients(3/15). Incidences of Grade 1 to 2 myelosuppression was observed in 1/15 patients, and Grade 1 to 2 elevation of hepatic enzyme was recorded in 1/15 patients. Adverse effects on the gastrointestinal tract were documented in 1/15 patients, and were Grade 1. No Grade 3-4 toxicities were diagnosed. No treatment related death was found. Conclusions: Thalidomide combined with chemotherapy was safe and mildly effective in treating patients with advanced colorectal cancer. However, further study should be conducted to clarify the effectiveness of this combination.

DEVELOPMENT OF CHINA LIGHT-DUTY VEHICLE TEST CYCLE

Yu Liu,Zhi Xin Wu,Hua Zhou,Han Zheng Nan Yu,Xiao Pan An,Jing Yuan Li,Meng Liang Li 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.5

Driving cycles provide a basis for vehicle development and calibration and also serves as the foundation for energy consumption and emissions certification of vehicles. This paper presents the China Light-Duty Vehicle Test Cycle (CLTC) developed by the China Automotive Technology & Research Center (CATARC). First, the important steps and technical routes toward the CLTC development process are summarized. Second, the specific CLTC development process is presented in detail, including the data acquisition and data analysis procedures, weighting factor development and driving cycle construction. Then, the main driving characteristics of the New European Driving Cycle (NEDC), the Worldwide Harmonized Light-Duty Vehicles Test Cycle (WLTC), the Federal Test Procedure (FTP-75), the CLTC and the actual collected data are compared. The CLTC has low average speed, a high idle speed ratio and more frequent acceleration and deceleration characteristics. Finally, 70 vehicles are t ested based on the NEDC, WLTC, and CLTC according to their legislative procedures in the vehicle emission laboratories of the CATARC and the manufacturers. The results show that the CLTC’s fuel consumption is much higher than that of the NEDC and WLTC, and CLTC can effectively reflect the actual fuel consumption of users.

In vitro Removal of Deoxynivalenol and T-2 Toxin by Lactic Acid Bacteria

Zhong-Yi Zou,Zhi-Fei He,Hong-Jun Li,Peng-Fei Han,Xiao Meng,Yu Zhang,Fang Zhou,Ke-Pei Ouyang,Xi-Yue Chen,Jun Tang 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6

Five strains of lactic acid bacteria were tested for their ability to remove deoxynivalenol (DON) and T-2toxin from MRS broth. The ability of Lactobacillus plantarum strain 102 (LP102) was the strongest among 5strains after incubation at 37oC for 72 h. The mode of removal was physical binding, rather than biotransformation. The abilities were not significantly different between when removing single toxin and when removing mixed toxins by viable cells of LP102. DON and T-2 toxin released from LP102 viable cell-toxin complexes were 28.22±1.55 and 35.42±2.02% of total bound toxins respectively after 3times of wash with posphate buffered saline, respectively,those were 4.59±0.86 and 5.59±1.47% after incubation with simulated gastric fluid (SGF) at 37oC for 4 h, and 6.86±0.81 and 9.04±1.13% after incubation with simulated intestinal fluid (SIF) at 37oC for 4 h, respectively.

Structure and bioactivity of triterpenoids from the stems of Schisandra sphenanthera

Cheng-Qin Liang,Rong-Hua Luo,Ju-Ming Yan,Yan Li,Xiao-Nian Li,Yi-Ming Shi,Shan-Zhai Shang,Zhong-Hua Gao,Liu-Meng Yang,Yong-Tang Zheng,Wei-Lie Xiao,Hong-Bin Zhang,Han Dong Sun 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.2

Two new triterpenoids, schisphendilactoneA and B (1 and 2), together with three known triterpenoids,were isolated from the stems of Schisandra sphenanthera. Their structures were elucidated by spectroscopic methods,and the absolute configuration of 1 was determined bysingle-crystal X-ray diffraction. Compound 2 showedmoderate inhibitory activity against SW480 cancer cellline, and compound 5 exhibited promising anti-HIV-1activity with EC50 value of 0.52 lg ml-1 and therapeuticindex value of 117.12.