인체 치은섬유모세포에 대한 니켈의 세포독성과 Ferulic Acid 및 Vitamin E의 독성경감효과

정윤상,한두석 원광대학교 치의학연구소 2004 圓光齒醫學 Vol.13 No.1

Nickel is major metal used in the nickel-chromium alloys of most orthodontic appliances and partial denture. But this metal is known to cause hypersensitivity, dermatitis and asthma. In addition, significant carcinogenic and mutagenic potentials has been demonstrated for compounds containing nickel. The purpose of this study was to develop the compounds repairing on the cytotoxicity of nickel in cultured human gingival fibroblasts. The cytotoxicity of nickel sulfide and nickel oxide in human gingival fibrobalsts treated with dose dependent nickel was measured by MTT assay for cell viability and XTT assay for cell adhesion activity. The cytotoxicity of ferulic acid itself also was investigated by the same cells and methods. The procedure of repairment in human gingival fibroblasts injuried by NiS_50 (nickel sulfide IC_50) and Ni0_50 (nickel oxide IC_50) was carried out to observe after treatment of ferulic acid by dose dependent manner. The 5×10⁴ cells/㎖ of human gingival fibroblasts in each well of 24 multidish were cultured. After 24 hrs, the cells were treated with solution of all groups. After the human gingival fibroblasts were cultured in same condition for 48 hrs, the colormetric methods were performed to evaluate the cytotoxicity. The light microscopic study was carried out to observe morphological changes of cultured human gingival fibroblasts. The results were as follows : 1. According to the MTT absorbance, nickel sulfide and nickel oxide were significantly decreased in 25μM and 50μM, respectively. IC_50 of nickel sulfide and nickel oxide were 138.1μM and 311.7μM, respectively. 2. According to the XlT absorbance, nickel sulfide was significantly decreased in 50 μM and nickel oxide was significantly decreased in 100μM, IC_50 of nickel sulfide and nickel oxide were 104.4μlM and 367.8μM, respectively. 3. MTT_50 and XTT_50 of ferulic acid were 2,130.3μM and 1,773.7μM, respectively. These results were determind to nontoxic by Borenfreund at., al. 4. The inhibitory effects of ferulic acid against nickel-induced cytotoxicity were significantly increased from 25μM ferulic acid, but inhibitory effects of 100μM ferulic acid was more decreased than that of 50μM ferulic acid. 5. Treatment of ferulic acid and vitamin E combination was more assumed an upward curve than that of ferulic acid only. 6. The cell numbers and cell shapes were repaired from 1μM ferulic acid. However 100μM ferulic acid showed degeneration again. These results suggest that ferulic acid and vitamin E may be decrease the cytotoxicity of nickel sulfide and nickel oxide in cultured human gingival fibroblasts.

상지의 분절성 운동 마비 증상을 나타낸 대상포진 감염 1예

정두신,이은아 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Herpes zoster is a viral disease that produces inflammatory lesions in the posterior root ganglia with pain and skin eruptions of affected ganglia. It has been rarely associated with motor paralysis of extremity. But involvement of motor nerve is in a small percentage(1-5%) in the herpes zoster infection. A 61-year-old woman presented with painful grouped vesicular eruptions on the right posterior neck and shoulder area. And she complained of weakness of shoulder joint and arm with painful skin lesions. Neurologic examination showed weakness of right shoulder muscles. EMG/NCV studies suggest cervical radiculopathy(C5, 6, 7). The cervical spine MRI study and CSF examination were carried at seven days after symptoms. The CSF study showed pleocytosis(WBC=144), increased protein level(103mg%), and positive antibody of HZV-Ig G & M. The motor weakness finding cleared forty days later. We report a patient of herpes zoster infection with segmental motor paresis of right shoulder muscles and positive CSF HZV-Ig G & M.

알코올 금단 발작 환자에서 진전 섬망 예방에 대한 Nimodipine과 Chlordiazepoxide의 효과 비교

정두신,양광익,이보람,방차옥,이태경,성기범,안무영,박형국 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Background & objectives : An increase of calcium influx during alcohol withdrawal state may contribute to neuronal hyperexitability, which cause delirium tremens(DT). Some investigators have found calcium channel blockers effects in prevention of DT in experimental animals. Therefore we evaluated the preventive effects of calcium channel blocker(nimodipine) and benzodiazepine(chlordiazepoxide) on the development of DT in alcohol withdrawal seizure(AWS) patients. Method : A total 59 patients with AWS were divided into three groups according to the management nimodipine-treated, chlordiazepoxide-treated, and control(no specific medication) groups. We compared the incidence rates of DT in the three groups. Results : Total incidence rate of DT was 30.5%(18 of 59 patients). There were 6 DTs of 18 patients(33.5%) in nimodipine-treated group, 4 of 21 patients(22.5%) in chlordiazpoxide-treated group, and 8 of 20 patients(44.0%) in control. Conclusion : The control group(44.0%) showed the highest incidence rate of DT. And nimodipine-treated(33.5%) and chlordiazepoxide-treated group(22.5%) were followed. However, this result failed to demonstrated statistically significant differences due to small numbers size.

제 6번 뇌신경 마비 증상으로 발현된 비소세포성 폐암 : 이차성 사대 전이 골수 변화 Secondary to metastatic clivus bone marrow change

정두신 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Isolated abducens nerve palsy usually occurs in the vasculopathic age group. It is often associated with diabetes mellitus, hypertension, and atherosclerosis. But also it occurred in the head trauma and skull base tumor. A 71-year-old man with diplopia was admitted. He had suffered from right temporo-orbital sided mild headache without nausea and vomiting. Physical and neurological examination revealed a right lateral rectus paralysis. Simple X-ray skull series showed unusual opacitic lesion on right side(Fig 1.). Brain MRI and temporal bone CT showed diffuse bone marrow change in right clivus(Fig 2, 3). He was discharged and transferred the Korea Cancer Center Hospital(KCCH). He was evaluated in department of oncology, KCCH. Chest CT finding was ill-defined margined lung cancer. The pathologic findings were non-small cell lung cancer. Ten months after follow-up, he was expired.

목회자의 직무만족과 그 요인에 관한 연구 : 목포지역 교회를 중심으로 Focused on churches in the district of Mokpo

정두배 대불대학교 2004 大佛大學校大學院 硏究論文集 Vol.3 No.-

The purpose of this study is to review the factors of christian pastors'job satisfaction and to know whether they are quite equal to their endowed pastoral duties under these factors or not. As analysis data, objective group is restricted to the pastors of protestant churches under the same religious background in Mokpo district. Though many pastors don't satisfy several factors which they have been given, they are inclined to meet these dissatisfied factors in response to a royal summons, so to speak, due to the reason they have become pastors by the selection of God. It is found that they have been quite equal to their endowed pastoral duties. the most part of pastors except for some pastors in large churches don't take salaries which amount to common salary level, but as they overcome a lot of upcoming conflicts in! the midst of human relations with the pastors who have all sort of characters, they have the satisfaction doing their endowed pastoral duties. I intended to take much more results through this study but I didn't carry out this study completely due to insufficient preceding research and measuring instruments. Nevertheless, I'd like 0to emphasize that pastors living their pastoral life as a man havehad the satisfaction doing their endowed pastoral duties in the midst of in sufficient environments and also we can understand they shows a model of pastoral life.

부천시 한 종합 병원의 응급실 진료 내용 분석

정두신 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Background & objectives : In recent, with the increasing need for medical treatment and changing life styles for personal health care, the need of emergency utilization for the patient seems to be growing. But there are few studies related with emergency room services in the secondary local general hospital. This study was conducted to obtain the basic clinical data of clinical contents of the emergency department utilization at a 250-bed local general hospital for systemic, efficient management of emergency medical services and the better medical planning. Method : This study is an analysis of 11,884 patients in the emergency department at a 250-bed local general hospital in Bucheon city Kyungki province from Mar. 1 1999 to Feb. 28 2000. The patient charts were reviewed and analyzed. Results : The results are follows : 1) Distributions of age and sex in the patients were frequent in 20-29(22.1%), 30-39(18.9%), 40-49(17.5%), under 9(16.6%) and males(60%) were more than females(40%). 2)Distributions of visiting hour in the patients were the most frequent in 8:00(PM)-0:00(AM). 3)Distributions of day of week in the patients were frequent Sunday, Saturday, and Friday in order but admission rates were frequent in Thursday(29.8%), Friday(27.7%), and Wednesday(27.4%). 4)In classifying disease and chief complaints by system, musculoskeletal, digestive, neurologic, and respiratory system were ranked. 5)The results of the patients were classified simple care and medication(75.07%), general ward admission(20.25%), ICU admission(3.89%), and emergency operation(0.29%). 6)The department distributions of the patients were internal medicine, orthopedic surgery, neurosurgery, pediatrics, and general surgery. Conclusion : These findings suggest that emergency room visit in a secondary general hospital was more frequent non-urgent with the sole purpose of a medication or simple care.

漢江流域 豪雨의 空間特性

鄭斗永,李在炯 全北大學校 1987 論文集 Vol.29 No.-

By the spectral analysis of many heavy rains' recorded data, at Han river basin, an emprical spectral density function model is presented for the spatial distribution of storm. Using the storm data which was augmented by the Stochastic correlation with it's neighbors, the multiquadric random ndom surface of total storm depth is constructed. And to separate the local components from it's regionals and find the regional charcteristics, a double Fourier analysis was applied to the total depths. The local components, storm residuals of each storm was assumed to be an homogeneous random field and investigated with it's autocorrelation function. For the practical application, isotropic was assumed which was identifyed with emprical data. Coefficients of normalized autocorrelation for all storms were shown similar appearance. Using this emprical result, an example of the radial spectral density function is presented which represents the spatial characteristics of heavyrains over Han-river basin during 1975~1983.

뇌경색 환자의 혈중 호모시스틴치 (예비 연구)

정두신,송인욱,조상걸,성기범,박형국,신현길,장성근 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

우울과 관련된 장애를 가진 환자들의 PAI 프로파일

두정훈,강종구,노승호,오상우 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.2

흰쥐의 감각신경절세포에 대한 Methylmercuric chloride의 독성효과

鄭榮吉,白承和,韓斗錫,柳道坤,朴承澤 한국전통의학연구소 1998 한국전통의학지 Vol.8 No.1

흰쥐의 배양 척수감각신경절세포에 대한 methylmercuric chloride(MMC)의 세포독성을 조사하기 위하여 여러 농도의 MMC가 포함된 배앙액에서 24시간 동안 배양한 후 MTI assay법에 의하여 세포의 생존율을 조사하였다. MMC는 농도에 비례하여 세포의 생존율을 감소시켰다. 또한 여러 농도의 MMC가 포함된 배앙액에서 흰쥐의 척수 감각신경절(dorsal root ganglion, DRG) 세포를 24시간 동안 처리한 결과 MTI_(50) 값이 25μM MMC에서 나타났다. MMC는 Borenfreund등(1988)의 독성판정기준에 의하여 흰쥐의 배앙 DRG 세포에 고독성인 것으로 나타났다. 이상의 결과는 MMC가 흰쥐의 배양 척수감각신경절세포에 강한 신경독성 효과가 있음을 제시하였으며 또한 배양신경세포는 약제의 효과를 검색하는데 효과적이었다. To examine the cytotoxic effect of methyl mercuric chloride(MMC) on the cultured rat spinal dorsal root ganglion(ORG) neurons, cell viability was measured by MTT assay after rat spinal ORG neurons were incubated with media containing various concentrations of MMC for 24 hours The results were as follows ; 1. MMC decresed cell viability of rat spinal ORG neurons in a dose-dependent manner. 2. MIT_(950) value was a 25uM MMC after spinal ORG neurons were cultured for 24 hours at various concentrations of MMC. 3. MMC was heighly toxic on the cultured ORG neurons by the toxic criteria of Borenfreund et al.(1988). From above the results, it is suggested that methylmercury involves in neurotoxicity by the decrease of cell viability in cultured rat spinal DRG neurons, and cultured neurons are effective in screening the putative agents.