담배 니코틴에 의한 사람 태아 성상세포에서 종양괴사인자(TNF-α)의 발현 억제작용

손일홍,이성익,양현덕,한선정,석승한,이재규,김재현,박주영,문형인,이성수,Son, Il-Hong,Lee, Sung-Ik,Yang, Hyun-Duk,Han, Sun-Jung,Suk, Seung-Han,Lee, Jai-Kyoo,Kim, Jae-Hyun,Park, Joo-Young,Moon, Hyung-In,Lee, Sung-Soo 대한화학회 2007 대한화학회지 Vol.51 No.3

니코틴은 사람 대식세포에서 interleukin 2 (IL-2)와 종양괴사인자 (tumor necrosis factor-alpha; TNF-α) 가 생성되는 것을 억제하는데, 이러한 억제작용은 cytokine 유전자 발현 중 전사단계에서 전사인자의 활성을 억제함으로써 일어난다. 이러한 니코틴의 면역반응 억제작용은 아프타성궤양 및 궤양성대장염, 알레르기성폐 포염, 건초열 등에서도 보고되고 있다. 만일 중추신경계에서도 위와 같은 니코틴의 면역억제 작용이 일어난 다면 다발성경화증과 같은 면역반응 매개질환의 치료에 새로운 전기가 마련될 수 있을 것이다. 본 연구에서 는 중추신경계의 여러 면역반응 매개질환의 병태생리에 대한 이해를 넓히고자, 이미 알려진 니코틴의 cytokine 생성억제가 사람 중추신경계의 성상세포에서도 일어남을 확인하고 그 억제기전을 밝히고자 하였다. 이를 위 하여 사람 태아 성상세포에 다양한 농도의 니코틴과 IL-1β를 처리한 다음 TNF-α mRNA의 발현 정도와 NF- κB의 활성을 비교, 분석하여 다음과 같은 결과를 얻었다. 1. 사람 태아 성상세포를 0.1-20 μg/ml의 니코틴으로 처리해 본 결과 10 μg/ml 이상의 농도에서 세포독성능이 나타나기 시작하였다. 2. 사람 태아 성상세포에 IL- 1β를 처리하면 2시간만에 TNF-α mRNA가 최대로 발현되었으며 그 이후로는 점진적으로 감소하였다. 3. 사 람 태아 성상세포를 1 및 0.1 μg/ml의 니코틴으로 전처리한 후 IL-1β로 자극한 군에서는 IL-1β 단독 처리군에 비해 TNF-α mRNA의 발현이 감소하는 양상을 보였다. 1 μg/ml의 니코틴을 처리한 경우에는 8시간 이후부터 TNF-α mRNA의 발현이 현저하게 감소하여 12시간에 최대로 감소하였다. 또한 0.1 μg/ml의 니코틴을 처리한 군에서는 24시간에 가장 현저하게 감소하였다. 4. 성상세포에 IL-1β로 처리한 군에서는 강력한 NF-κB의 활성 을 확인할 수 있었으며, 니코틴을 전처리하고 IL-1β 자극한 군에서는 NF-B의 활성이 감소하였다. 결론적으로 일정농도 이상의 니코틴은 세포독성효과를 나타내나 적정한 농도와 시간 경과후 니코틴은 사람 태아 성상세포에서 IL-1β에 의해 유도되는 TNF-α의 발현 감소를 유도하며, 이는 NF-κB의 활성을 감소시킴으로써 나타난다고 생각된다. The Tumor necrosis factor-α, (TNF-α), is involved in the pathogenesis of multiple sclerosis and contributes to the degeneration of oligodendrocytes as well as neurons. Nicotine has been found to have immunosuppressive and inflammation-suppressing effects. Astrocytes, the major glial cells in the CNS, are capable of producing TNF-α at both the mRNA and protein levels in response to interleukin-1 (IL-1) or TNF-α. Nicotine has been shown to influence glial cell functions. To order to explore the role of astrocytes in the production of TNF-α, astrocytes were pretreated with nicotine and are stimulated with IL-1β to determine their effects on TNF-α production. The results are as follows. Cytotoxic effects of nicotine on human fetal astrocytes were noted above 10 μg/ml of nicotine. The effect of IL-1β on TNF-α mRNA expression in primary cultured human fetal astrocytes was maximal at 2 h after IL- 1β(100 pg/ml) treatment. Human fetal astrocytes were pretreated with 0.1, 1, and 10 μg/ml of nicotine and then stimulated with IL-1β (100 pg/ml) for 2 h. The inhibitory effect of nicotine on expressions of TNF-α mRNA in human fetal astrocytes with pretreated 0.1 μg/ml of nicotine is first noted at 8 hr, and the inhibitory effect is maximal at 12 h. The inhibitory effect at 1 μg/ml of nicotine is inhibited maximal at 24 h. Nicotine at 0.1, 1 and 10 μg/ml concentrations significantly inhibits IL-1β-induced NF-κB activation. Collectively, this study indicates that nicotine might inhibit the expression of TNF-α in activated human fetal astrocytes.

거습복합방(祛濕複合方)이 류마토이드 관절염에 미치는 영향

한주원 ( Joo Won Han ),송영일 ( Young Il Song ),윤일지 ( Il Ji Yoon ),오민석 ( Min Suck Oh ) 한방재활의학과학회 2006 한방재활의학과학회지 Vol.16 No.3

Objectives : This study was carried out to know the inhibitory effect of Kuseupbokhap-bang(Qush.fuhe-f.ng; KC) on rheumatoid arthritis in human fibroblast-like synoviocytes(hFLSs). Methods : For this purpose, experiments were performed to measure the cytotoxicity against hFCs and the production of pro-inflammatory cytokines in hFLSs and the production of NO, ROS. Results : KC had no cytotoxicity against hFCs and reduced the production of pro-inflammatory cytokines TNF-α, IL-6, IL-8, IL-1β in hFLSs, increased the production of TIMP-1. As well as KC reduced the production of MMP-3, NOS-II, ICAM-1, the production of Nitric oxide and ROS, and the proliferation of hFLSs in proportion to the concentration of KC significantly. Conclusions : Comparison of the results for this study showed that KC had immunomodulatory effects of suppressing or enhancing.

Effect of Cytokines and bFGF on the Osteoclast Differentiation Induced by 1α,25-(OH)<SUB>2</SUB>D<SUB>3</SUB> in Primary Murine Bone Marrow Cultures

Han-Jung Chae,Jang-Sook Kang,Byung-Gwan Bang,Seoung-Bum Cho,Jo-IL Han,Joo-Young Choi,Hyung-Min Kim,Soo-Wan Chae,Hyung Ryong Kim 대한생리학회-대한약리학회 1999 The Korean Journal of Physiology & Pharmacology Vol.3 No.6

<P> Bone is a complex tissue in which resorption and formation continue throughout life. The bone tissue contains various types of cells, of which the bone forming osteoblasts and bone resorbing osteoclasts are mainly responsible for bone remodeling. Periodontal disease represents example of abnormal bone remodeling. Osteoclasts are multinucleated cells present only in bone. It is believed that osteoclast progenitors are hematopoietic origin, and they are recruited from hematopoietic tissues such as bone marrow and circulating blood to bone. Cells present in the osteoclast microenvironment include marrow stromal cells, osteoblasts, macrophages, T-lymphocytes, and marrow cells. These cells produce cytokines that can affect osteoclast formation. In vitro model systems using bone marrow cultures have demonstrated that IL-1β, IL-3, TNF-α, bFGF can stimulate the formation of osteoclasts. In contrast, IL-4 inhibits osteoclast formation. Knowledge of cytokines and bFGF that affect osteoclast formation and their capacity to modulate the bone-resorbing process should provide critical insights into normal calcium homeostasis and disorders of bone turnover such as periodontal disease, osteoporosis and Paget s disease.

Nicotine Suppresses TNF-${\alpha}$ Expression in Human Fetal Astrocyte through the Modulation of Nuclear Factor-${\kappa}B$ Activation

Son, Il-Hong,Park, Yong-Hoon,Yang, Hyun-Duk,Lee, Sung-Ik,Han, Sun-Jung,Lee, Jai-Kyoo,Ha, Dae-Ho,Kang, Hyung-Won,Park, Joo-Young,Lee, Sung-Soo The Korean Society of Toxicogenomics and Toxicopro 2008 Molecular & cellular toxicology Vol.4 No.2

Parkinson's disease (PD) progresses severely by a gradual loss of dopaminergic neurons in the substantia nigra (SN). Epidemiological studies showed that the incidences of PD were reduced by smoking of which the major component, nicotine might be neuroprotective. But the function of nicotine, which might suppress the incidences of PD, is still unknown. Fortunately, recently it was reported that a glial reaction and inflammatory processes might participate in a selective loss of dopaminergic neurons in the SN. The levels of tumour necrosis factor (TNF)-${\alpha}$ synthesised by astrocytes and microglia are elevated in striatum and cerebrospinal fluid (CSF) in PD. TNF-${\alpha}$ kills the cultured dopaminergic neurons through the apoptosis mechanism. TNF-${\alpha}$ release from glial cells may mediate progression of nigral degeneration in PD. Nicotine pretreatment considerably decreases microglial activation with significant reduction of TNF-${\alpha}$ mRNA expression and TNF-${\alpha}$ release induced by lipopholysaccharide (LPS) stimulation. Thus, this study was intended to explore the role of nicotine pretreatment to inhibit the expressions of TNF-${\alpha}$ mRNA in human fetal astrocytes (HFA) stimulated with IL-$1{\beta}$. The results are as follows: HFA were pretreated with 0.1, 1, and $10{\mu}g/mL$ of nicotine and then stimulated with IL-$1{\beta}$ (100 pg/mL) for 2h. The inhibitory effect of nicotine on expressions of TNF-${\alpha}$ mRNA in HFA with pretreated $0.1{\mu}g/mL$ of nicotine was first noted at 8hr, and the inhibitory effect was maximal at 12 h. The inhibitory effect at $1{\mu}g/mL$ of nicotine was inhibited maximal at 24 h. Cytotoxic effects of nicotine were noted above $10{\mu}g/mL$ of nicotine. Moreover, Nicotine at 0.1, 1 and $10{\mu}g/mL$concentrations significantly inhibited IL-$1{\beta}$-induced TF-${\kappa}B$ activation. Collectively, these results indicate that in activated HFA, nicotine may inhibit the expression of TNF-${\alpha}$ mRNA through the pathway which suppresses the NF-${\kappa}B$ activation. This study suggests that nicotine might be neuroprotective to dopaminergic neurons in the SN and reduce the incidences of PD.

單純보上에서 一定速度를 갖는 剛體系의 振動解析

朴日柱,陳鍾泰,尹漢翼 東義大學校産業技術開發硏究所 1992 産業技術硏究誌 Vol.6 No.-

The vibration problem of a two-dimensional rigid body system on a simple beam is studied. The rigid body system is supported by the suspension units with stiffness and damping, and it has constant velocity on a beam. The deformations of the beams is represented by their corresponding eigenfunction series. The Runge-Kutta method is used in numerical analysis. 1. Regardless of damping, as the speed reduces, the maximum deflection of rigid body system increases, and as the speed increases, the maximum deflection of rigid body system reduces. 2. As the speed reduces, the deflection of rigid body system decreaces at endpoint of the simple beam from C=0 to C=100 N·s/m. And in case of C=1700 N·s/m, the deflection of rigid body system at endpoint of the simple beam is smallest at midspeed 22.22 m/s. 3. In case of undamping(C=O), the maximum deflection of rigid body system is moved from midpoint to endpoint of the simple beam as the speed increases. 4. As the damping coefficient increases, the deflection of rigid body system increases very small at the initial portion of the simple beam, but the maximum deflection reduces. 5. As the damping coefficient increases, the maximum deflection of rigid body system is moved from endpoint to midpoint of the simple beam.

Platelet-Rich Plasma (PRP)가 토끼 척골의 분절 결손의 치료로 이식된 동종 장관골의 골유합에 미치는 효과

송주현,정진영,강용구,이한용,손문익,서상일 대한골절학회 2003 대한골절학회지 Vol.16 No.3

목적: 골종양 환자나 외상, 골수염, 인공관절 치환술 등의 환자 등의 환자 치료에 많이 사용하는 장관골의 동종 이식에 대한 PRP (Platelet-Rich Plasma)의 효과를 토끼를 이용한 실험으로 알아보고자 하였다. 대상 및 방법: 20마리의 토끼를 대상으로 각각에 일련번호를 붙이고, 각 토끼의 양측 척골에 약 2㎝ 길이의 골편을 얻어 장관골 결손을 만들고 동시에 동종 장관골을 얻어, 홀수 토끼의 척골 골편을 짝수 토끼의 골결손 분위에 이식하고, 반대로 짝수 토끼의 척골 골편을 홀수 토끼의 골결손 분위에 이식하였다. 그 후 각 토끼의 좌측 척골 이식 부위에는 아무런 처치를 하지 않고 대조군으로 삼고, 우측 척골 이식부에는 PRP 0.7cc를 처치하여 실험군으로 삼았다. 술 후 0, 4, 8, 12주에 골이식부에 대한 방사선 촬영을 통하여 골유합 성적을 평가 비교하였다. 결과: 실험군의 척골 분절 결손부에서는 대조군에서보다 동종 분절골 이식부의 골유합이 방사선학적으로 향상된 것을 관찰 할 수 있었다. 결론: 동종 장관골 분절 이식시 PRP를 병용하여 사용할 경우 더욱 효과적인 신생조직 형성을 유도할 수 있으며, 임상에도 유용하게 적용할 수 있으리라 사료된다. Purpose: The purpose of this study was to evaluate the effect of Platelet-Rich Plasma(PRP) on the healing of the allograft for the treatment of the segmental bone defect of the ulna in Rabbits. Materials and Methods: About 2 ㎝-sized segmental bone defects were created on both ulna of twenty rabbits. The rabbits were divided into two groups, even and odd number groups after numbering them from 1 to 20. The segmental bone from the odd numbered animal was transplanted to the even numbered animal, and the even numbered to the odd numbered. The left side of the ulna was grafted with a segmental allograft only. The right side of the ulna was grafted with a segmental allograft and 0.7cc of PRP. Radiographs obtained at 0, 4, 8, and 12 weeks postoperatively were graded for radiologic union. Result: The use of the combination of PRP and segmental allograft demonstrated improved healing on radiographic study compared with that demonstrated after use of allograft alone. Conclusion: The results of the study suggests that the use of the combination of PRP and segmental allograft can be considered as an alternative method to manage the segmental defect of the long bone.

암센서용 TiO2/Au/CNT 나노복합물의 전기화학적 검출

홍성미, 김홍일, 김한주, Wang, Xuemei, 박수길 충북대학교 건설기술연구소 2008 충북대학교 건설기술연구소 논문집 Vol.27 No.1

Demand of rapid detection for cancer, it is necessary that high sensitivity, specificity, low cost, and simple method. CNT and metal nano materials are widely used to elecate efficient. CNT have various advantage, and can be composed with metal nano materials. Especially, Titanium oxide and gold nano particles are focused on sensor for detection of cancer, it can elecate electron transition feeiciency between electrode surface and target materials, and biological functionalization of CNT. It means that nano material is composed each other. In this study, electrodes are considered materialsd and their chracteristic for cancer sensor.

BMS 수리모형하에서 시스템의 수리효과 및 고장강도함수의 모수추정

윤원영,정석주,정일한,김종운,정상욱 한국경영과학회 2000 韓國經營科學會誌 Vol.25 No.4

Estimation problems of parameters of the failure process and the repair effect in repairable systems are considered. We propose estimation procedures in repairable systems without preventive maintenances. The fallure process is modeled by a proportional age reduction model (Brown. Mahoney, Sivazlian [5]) which is able to consider both aging and repair effects. Maximum likelihood method is used to estimate the repair effect and parameters of intensity function simultaneously. Simulations are performed to evaluate the accuracy of estimators. A numerical example is also presented.

NeX-Pert를 활용한 프로젝트 관리에 관한 연구

오기환,정한일,엄주태 대전대학교 산업기술연구소 1999 산업기술연구소 論文集 Vol.10 No.2

In recent, as the application domain of information system becomes large, the project management concept is applied to not only the large-scale construction projects but also the development of an information system. Most of the ERP vendors release methodologies and software for project management. The success stories of applying the project management concept to the large-scale construction projects and development of an information system have been reported. However a case study for applying the project management technology to small-scale projects was not reported yet. This paper is intended to show the case study for applying the project management technology to small-scale project using the project management software, NeX-Pert.

교류전해에칭용 에틸렌글리콜의 알루미늄박판의 부식억제 효과

박성민, 양정진, 김홍일, 김한주, 김성한, 신진식, 박수길 충북대학교 건설기술연구소 2008 충북대학교 건설기술연구소 논문집 Vol.27 No.1

In this study, additions of aluminium foil eching characteristics were compared with sulfuric acid for capacitor. sulfuric acid and ethylene glycol is used as addition, characteristics of funtional group from addition were guessed by comparing of aluminium foil eching characteristics. When sulfuric acid was added in HCI etching solution, corrosion of aluminum metal was inhibited by the chemical adsorption of sulfate ion, when ethylen glycol was added in HCI etching solution, corrosion inhibited by the chemical adsorption of OH­^ion.