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방병관,강정숙,채한정,김형룡 원광대학교 생체재료·매식연구소 1998 원광생체재료·매식 Vol.7 No.1
Nitric oxide(NO) is known to be implicated in bone metabolism, especially as a mediator of cytokine effects for remodeling of bone tissue. In this study we examine whether NO affects osteoblast activation of osteoblast or osteoclastic differentiation in primary mouse osteblast-like and osteosarcoma ROS 17/2.8 cells. Primary osteoblast and ROS 17/2.8 cells release NO upon stimulation of interleukin-1β, tumour necrosis factor-α, and interferon-γ. Sodium nitroprusside, a donor of NO, increases the activity of alkaline phosphatase in ROS 17/2.8 cells as well as the number of calcified nodule formations in primary mouse osteoblast-like cells. Sodium nitroprusside also completely also completely inhibites 1 α,25-(OH)_2D_3-induced osteoclast genration in a high concentration (100 μM). However, low concentrations of sodium nitroprusside (3∼30 μM) significantly increase the generation of osteoclasts. These results that NO appears to be an important regulatory molecule in the processes of bone formation and resorption. Hence, NO may be involved in the pathogenesis of bone loss in diseases associated with cytokine activation such as periodontal disease and rheumatoid arthritis.
Tyrosinase 저해제를 생성하는 방선균 F-97의 분리 및 동정
방병호 ( Byung Ho Bang ),이문수 ( Moon Soo Rhee ),김관필 ( Gwan Pil Kim ),김진오 ( Jin O Kim ),이동희 ( Dong Heui Yi ) 한국식품영양학회 2009 韓國食品營養學會誌 Vol.22 No.1
본 연구에서는 독성이 적고, 활성이 높으면서 안정한 새로운 tyrosinase 저해제를 미생물을 대상으로 얻고자 tyrosinase 저해제를 강하게 분비하는 방선균 F-97을 토양으로부터 분리하였다. 이 균의 기균사는 회색의 나뭇가지형(tree type)이며, 포자의 끝부분이 유선형으로 회전되어 있고, 포자 표면은 구형의 smooth형으로 분류되었다. 세포벽의 DAP 성분은 LL-DAP이었으며, 세포균체의 당 성분은 검출되지 않았다. 기타 배양학적 및 생리적 특성을 종합한 결과 Streptomyces aburaviensis 또는 근연균으로 추정된다. In order to obtain a non-toxic and more active and stable microorganism-produced tyrosinase inhibitor, we isolated actinomycetes F-97, a producer of tyrosinase inhibitor, from soil. The aerial hyphae of this strain were gray in color with tree types. Under the microscopic examination, the isolate formed a spiral aerial spore mass with a smooth surface. The analysis of cell wall acid hydrolysate of the isolate revealed the presence of LL-diaminopimelic acid(LL-DAP). No specific sugar was detected. From these results and the cultural and physiological characteristics described in the Bergey`s Manual, actinomycetes F-97 was identificated as, or best-matched to, Streptomyces aburaviensis.
Han-Jung Chae,Jang-Sook Kang,Byung-Gwan Bang,Seoung-Bum Cho,Jo-IL Han,Joo-Young Choi,Hyung-Min Kim,Soo-Wan Chae,Hyung Ryong Kim 대한생리학회-대한약리학회 1999 The Korean Journal of Physiology & Pharmacology Vol.3 No.6
<P> Bone is a complex tissue in which resorption and formation continue throughout life. The bone tissue contains various types of cells, of which the bone forming osteoblasts and bone resorbing osteoclasts are mainly responsible for bone remodeling. Periodontal disease represents example of abnormal bone remodeling. Osteoclasts are multinucleated cells present only in bone. It is believed that osteoclast progenitors are hematopoietic origin, and they are recruited from hematopoietic tissues such as bone marrow and circulating blood to bone. Cells present in the osteoclast microenvironment include marrow stromal cells, osteoblasts, macrophages, T-lymphocytes, and marrow cells. These cells produce cytokines that can affect osteoclast formation. In vitro model systems using bone marrow cultures have demonstrated that IL-1β, IL-3, TNF-α, bFGF can stimulate the formation of osteoclasts. In contrast, IL-4 inhibits osteoclast formation. Knowledge of cytokines and bFGF that affect osteoclast formation and their capacity to modulate the bone-resorbing process should provide critical insights into normal calcium homeostasis and disorders of bone turnover such as periodontal disease, osteoporosis and Paget s disease.
Jeong, Eun-Ja,Rhee, Moon-Soo,Kim, Gwan-Pil,Lim, Ki-Hwan,Yi, Dong-Heui,Bang, Byung-Ho The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.1
We isolated Bacillus sp. SH-517 from decomposed chicken feathers at a local poultry plant. This strain produced a keratinase that degrades poultry feathers and therefore will be very valuable for industrial use. Most feathers were degraded by the strain within 40 h at $40^{\circ}C$ by shaking culture (180 rpm). The keratinase from the culture medium of Bacillus sp. SH-517 was purified by centrifugation, 30-80% ammonium sulfate fractionation, twin-column DEAE-cellulose ion exchange chromatography and Sephadex G-150 gel filtration, to obtain a purified keratinase at 10.82% yield and 14-fold overall purification. The purified enzyme had a specific activity of 825 U/mg. A single protein band was shown on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE). The molecular weight of the keratinase from Bacillus sp. SH-517 was estimated as 51 kDa. The optimum pH and temperature for the enzyme reaction were 7.5 and $40^{\circ}C$, respectively. The enzyme remained stable over the pH range from 4.0 to 9.0 and at temperatures below $50^{\circ}C$. Proteins such as milk casein and chicken feathers were easily hydrolyzed by this enzyme. The enzyme activity was significantly inhibited by $Hg^{2+},\;Ag^{2+}$, ethylene diamine tetraacetic acid (EDTA) and ethylene glycol tetraacetic acid (EGTA), but slightly stimulated by $K^+$ and $Na^+$.
Eun Ja Jeong,Moon Soo Rhee,Gwan Pil Kim,Ki Hwan Lim,Dong Heui Yi,Byung Ho Bang 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.1
We isolated Bacillus sp. SH-517 from decomposed chicken feathers at a local poultry plant. This strain produced a keratinase that degrades poultry feathers and therefore will be very valuable for industrial use. Most feathers were degraded by the strain wi
다제내성 Acinetobacter baumannii에 유효한 방선균 B-51의 탐색 및 이 균주가 생산하는 항생물질 발효 최적 배양 조건
이문수 ( Moon Soo Rhee ),김관필 ( Gwan Pil Kim ),방병호 ( Byung Ho Bang ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.1
With the increase of the use of antibiotics and invasive procedures, infections caused by multidrug-resistant Acinetobacter baumannii(MRAB) are increasing. We screened the antibiotic producing strain B-51 for antibacterial activity against MRAB from the soils and studied the effects of culture medium on the antibiotic production of B-51. The medium conditions for maximum antibiotic productivity of B-51 was 2% glycerol, 0.5% soybean meal, 0.01% CaCl2, 0.01% MgSO4,7H2O and 0.01% KH2PO4 at an initial pH of 6.0, at 30℃ for 76 h.
Transition Metal Induces Apoptosis in MC3T3E1 Osteoblast Evidence of Free Radical Release
Han-Jung Chae,Soo-Wan Chae,Jang-Sook Kang,Dong-Hyeon Yun,Byung-Gwan Bang,Mi-Ra Kang,Hyung-Min Kim,Hyung-Ryong Kim 대한생리학회-대한약리학회 2000 The Korean Journal of Physiology & Pharmacology Vol.4 No.1
<P> Transition metal ions including Se<SUP>2</SUP>, Cd<SUP>2</SUP>, Hg<SUP>2</SUP> or Mn<SUP>2</SUP> have been thought to disturb the bone metabolism directly. However, the mechanism for the bone lesion is unknown. In this study, we demonstrated that MC3T3E1 osteoblasts, exposed to various transition metal ions; selenium, cadmium, mercury or manganese, generated massive amounts of reactive oxygen species (ROS). The released ROS were completely quenched by free radical scavengers-N-acetyl cysteine (NAC), reduced glutathione (GSH), or superoxide dismutase (SOD). First, we have observed that selenium (10μM), cadmium (100μM), mercury (100μM) or manganese (1 mM) treatment induced apoptotic phenomena like DNA fragmentation, chromatin condensation and caspase-3-like cysteine protease activation in MC3T3E1 osteoblasts. Concomitant treatment of antioxidant; N-acetyl-L-cysteine (NAC), reduced-form glutathione (GSH), or superoxide dismutase (SOD), prevented apoptosis induced by each of the transition metal ions. Catalase or dimethylsulfoxide (DMSO) has less potent inhibitory effect on the apoptosis, compared with NAC, GSH or SOD. In line with the results, nitroblue tetrazolium (NBT) stain shows that each of the transition metals is a potent source of free radicals in MC3T3E1 osteoblast. Our data show that oxidative damage is associated with the induction of apoptosis in MC3T3E1 osteoblasts following Se<SUP>2</SUP>, Cd<SUP>2</SUP>, Hg<SUP>2</SUP> or Mn<SUP>2</SUP> treatment.