Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

Effects of Phenytoin and Diazepam on the Seizure Activity in the Cortical Dysplasia Animal Models

Kim, Si-Hyung,Choi, In-Sun,Cho, Jin-Hwa,Park, Eun-Ju,Jang, Il-Sung,Choi, Byung-Ju,Kim, Hyun-Jung,Kim, Young-Jin,Nam, Soon-Hyeun The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Dysplasia-associated seizure disorders are markedly resistant to pharmacological intervention. Relatively little research has been conducted studying the effects of antiepileptic drugs(AEDs) on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate(MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of AEDs in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injucted control animals. Field potential recordings were used to monitor amplitude and numbers population spikes, and paired pulse inhibition in response to stimulation of commissural pathway. Two commonly used AEDs were tested: diazepam 5, 2.5mg/kg;phenytoin 40, 60mg/kg. Diazepam(DZP) and phenytoin(PHT) reduced the amplitude of population spike in control and MAM-exposed rats. However, the amplitude of population spike was nearly eliminated in control rats as compared to the MAM-exposed rats. Pharmaco-resistance was tested by measuring seizure latencies in awake rats after pilocarpine administration(320mg/kg, i.p.) with and without pretreatment with AEDs. Pre-treatment with PHT 60 mg prolonged seizure latency in control rats, but not in MAM-exposed animals. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard AEDs assessed in vivo. These data suggest that animal model with cortical dysplasia can be used to screen the effects of potential AEDs.

(±)-Camphor가 ICR 마우스 수컷의 간 cytochrome P450 효소 활성에 미치는 영향

오은경,박형건,배기현,최옥진,최은경,최창근,한진희,정태천 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.4

Effects of (±)-camphor on liver cytochrome P450 enzymes were investigated in male ICR mice. Mice were treated intraperitoneally with 0, 200, 400 and 800 mg/kg of (±) -camphor in corn oil for 3 consecutive days. Twenty four hr after the final treatment, the animals were subjected to necropsy. The activities of serum aspartate aminotransferase and serum alanine aminotransferase were slightly changed by the treatment with (±)-camphor at the doses used. Administration o(±)-camphor to mice significantly induced the hepatic activities of pentoxyresorufin O-depentylase and benzyloxyresorufin O-debenzylase and weakly induced ethoxyresorufin O-deethylase in dose-dependent manners. The present results suggested that (±)-camphor might act as a relatively specific inducer of hepatic cytochrome P450 213 in male ICR mice.

小兒銀杏中毒症 1例

崔衡鎭,金榮昌 순천향대학교 1983 논문집 Vol.6 No.4

Three year-old male child was admitted with chief complains of vomiting and tonic & clonic convulsive attack after three hours taking fresh fruits of Ginkgo biloba L.. He was treated with anticonvulsive drugs and 'fluid infusion, recovered completely and discharged in fine condition two days later. Brief review of related literatures were presented.

SOM 신경망을 이용한 심전도 검출에 관한 연구

崔塡亨,柳宗賢,白承和 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - In This study, proposes a self-organizing QRS-wave detection algorithm for ECG using Kohonen Neural Networks and a new preprocessing algorithm for R-point detection. The implemented ECG reading system consists of a preprocessor, a SOM and a QRS-detector. The preprocessor detect R-point in the ECG. The SOM organizes training vectors into a pattern book, and the QRS-detector executes Q and S-point detection with the pattern book. Without an averaging process permits a faster operation and applying a nonlinear peak detector to a preprocessor. The self-organizing ability of SOM has proven to be a good solution for recognizing and organizing QRS-wave patterns. Moreover, The SOM prevents an loss of information such as signal distortion due to a mathematical transform. The QRS-detector based on the pattern book from the SOM executes an efficient ECG reading compared to the other methods.

韓國産 植物凝集素 Falcata japonica K. 의 法醫學的 利用에 關한 硏究

崔衡鎭 大韓法醫學會 1988 대한법의학회지 Vol.12 No.2

In order to find our whether the extract of Korean native F.japonica which is known as A₁-lectin could be used in typing of blood stain and various body secretion and its stain for the subject of the judgement in, the field of legal-medicine, agglutinating inhibition test by human saliva, semen and vaginal secretion was performed. The results could be summarized as follows: 1. Agglutinating activity of F.japonica extract was specifically inhibited by saliva, semen, vaginal secretion of A₁ and A₁B type secretor. 2. Agglutinating activity of F.japonica extract was specifically inhibited by blood stain of A₁ and A₁B type and also by saliva stain, semen stain of secretor type. 3. Bloods of rats were classified into two groups, such as agglutinated and nonagglutinated groups by use of F.japonica extract. 4. Agglutinating activity of F.japonica extract was also specifically inhibited by use of agglutinated group of rat blood and blood stain.

폐모세포종 : 1례보고 A Case Report

최영호,서홍주,임영혁,김정중,박성강,이석기,임진수,김은규 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Pulmonary blastoma is a very rare primary neoplasm of the lung. We experienced a case of pulmonary blastoma in a 40 years old man. The mediobasal segmentectomy of the right lower lobe was performed. The patient was discharge on the postoperative days 6. There is no evidence of recurrence or metastasis on followup study during 7 months period after operation.

Lesch-Nyhan syndrome 환아의 oral self-mutilation 에 대한 증례보고

최병재,최형준,이제호,전진용 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.1

1. 하순에 심한 self-mutilation wound를 야기한 Lesch-Nyhan Syndrome 환아에서 상하악 유전치에 치관부 절단 및 치수 절세술을 시행하여 치아에 의한 자해를 방지하였다. 2. 장치를 이용한 치료가 어려운 경우 발치가 고려되기도 하나 본 증례에서는 발치보다 보존적인 술식으로 만족할 만한 결과를 얻었다. Self-injurous behavior is defined as deliberate harm to one's own body without suicidal intent. It usually occurs as head banging or hitting, skin cutting, or finger biting and includes ocular, genital and oral self-mutilation. Lesch-Nyhan syndrome is a rate X-linked recessively inherited disorder, caused by complete absence of hypoxanthin-guanine phosphoribosyl transferase(HPRT) activity. Clinical presentation is characterized by mental retardation, chorea, athetosis, hyperuricemia, uricosuria and self-mutilating behavior. In these patients, the most typical feature is loss of tissue from biting themselves, even though they are not insensitive to pain. The dental management of self-mutilation includes treatment with appliances such as soft mouthgrard or lip bumper, extraction of all the teeth, and orthognathic surgery. We report a 25-month-old boy who was a known case of Lesch-Nyhan syndrome and presented with severe self-mutilation wound on his lower lip. Vital pulpectomy and coronal resection was done as a more conservative approach than extracting all primary anterior teeth. Due to maintaining the root portion of the teeth in the bone, it is expected that the normal growth of the alveolar bone will be achieved.

온도; pH, 염분 내성 및 충격이 개불, Urechis unicinctus 유생에 미치는 영향

최상덕,김호진,라성주,정형택,윤호섭,신종암,이원교 國立 麗水大學校 環境問題硏究所 1999 環境硏究論文集 Vol.2 No.-

The effect of different temperature, salinity and pH on survival rate of Urechis unicinctus's swimming embryo was examined to develop the method for the efficient seed production. In addition to, this study was designed to investigate the possibility of pollutant marker diagnosis of marine pollutions by the change in different temperature, salinity and pH. Treatment was carried out whit different temperature (5˚C, 10˚C, 17˚C, 20˚C, 25˚C,), salinity(0‰, 10‰, 20‰, 30‰, 34‰, 40‰) and pH(4, 6, 7.2, 8.2, 9.2, 10). All the swimming embryo of the temperature experiment was survived within all experiment section, but the others experiment were not. At the temperature-shock and tolerance experiment ranging from 5˚C to 25˚C the survival rate was occurred: 75%, 95%, 100%, 100%, 50%, at the ph-Shock experiment ranging from 4 to 10, the survival rate was occurred: 0%, 50%, 85%, 100%, 60%, 0%, at the pH-tolerance experiment ranging 4 to 10 the survival rate was occurred : 0%, 75% 90%, 100%, 80%, 0%, and at the salinity-shock experiment ranging from 0% to 40% the survival rate was occurred : 0% 10% 90% 95% 100% 0% , at the salinity-tolerance experiment ranging from 0% to 40% , the survival rate was occurred : 0% 0% 90% 100% 100% 0%. These results suggest, that the survival rate of larvae of Urechis unicinctus was the highest at 17˚C, 34%. pH8.2 and this Urechis unicinctus could be live at the zone of euryceious and zone of euryhalines