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비 방사선 in situ hybridization과 confocal scanning microscope를 이용한 HCV에 감염된 human liver cell에서의 mRNA의 연구
정선우,김덕훈 國立昌原大學校 基礎科學硏究所 1994 基礎科學硏究所論文集 Vol.6 No.-
'In situ hybridization' has been used in searching the specific nucleic acid of the mRNA and viral DNA in the cell and tissue. For in situ hybridization, the use of a non-radioactive probe was considered as a good method. Because, the method provided excellent resolution of probe stability and personal safety. This study was performed by using the T-T dimered DNA as the non-radioactive probe in the human liver tissue infected by hepatitis C virus (HCV). The results were as follows. 1. The positive reaction of specific mRNA was detected from the hepatocyte. 2. Using the confocal scanning microscope and IBAS, the positive reaction wite was very evident. But, the negative reaction was not appeared in several cells. 3. Among Kupffer's cells, endothelial cells, and other cells, some were positively stained. 4. The sinusoids, central veins, hepatic arteries, bile ducts and connective tissues presented the negative reaction. 5. In the hepatocyte, the positive reaction was appeared to heterogenous in the nucleus. ‘In situ hybridization' has been used in searching the specific nucleic acid of the mRNA and viral DNA in the cell and tissue. For in situ hybridization, the use of a non-radioactive probe was considered as a good method. Because, the method provided excellent resolution of probe stability and personal safety. This study was performed by using the T-T dimered DNA as the non-radioactive probe in the human liver tissue infected by hepatitis C virus (HCV). The results were as follows. 1. The positive reaction of specific mRNA was detected from the hepatocyte. 2. Using the confocal scanning microscope and IBAS, the positive reaction site was very evident. But, the negative reaction was not appeared in several cells. 3. Among Kupffer's cells, endothelial cells, and other cells, some were positively stained. 4. The sinusoids, central veins, hepatic arteries, bile ducts and connective tissues presented the negative reaction. 5. In the hepatocyte, the positive reaction was appeared to heterogenous in the nucleus.
Woo, Jae Hoon,Ha, Tae-Woo,Kang, Jae-Seon,Hong, Jin Tae,Oh, Ki-Wan The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.1
Angelicae Gigantis Radix (AGR, Angelica gigas) has been used for a long time as a traditional folk medicine in Korea and oriental countries. Decursinol angelate (DCA) is structurally isomeric decursin, one of the major components of AGR. This study was performed to confirm whether DCA augments pentobarbital-induced sleeping behaviors via the activation of $GABA_A$-ergic systems in animals. Oral administration of DCA (10, 25 and 50 mg/kg) markedly suppressed spontaneous locomotor activity. DCA also prolonged sleeping time, and decreased the sleep latency by pentobarbital (42 mg/kg), in a dose-dependent manner, similar to muscimol, both at the hypnotic (42 mg/kg) and sub-hypnotic (28 mg/kg) dosages. Especially, DCA increased the number of sleeping animals in the sub-hypnotic dosage. DCA (50 mg/kg, p.o.) itself modulated sleep architectures; DCA reduced the counts of sleep/wake cycles. At the same time, DCA increased total sleep time, but not non-rapid eye movement (NREM) and rapid eye movement (REM) sleep. In the molecular experiments. DCA (0.001, 0.01 and $0.1{\mu}g/ml$) increased intracellular Cl- influx level in hypothalamic primary cultured neuronal cells of rats. In addition, DCA increased the protein expression of glutamic acid decarboxylase ($GAD_{65/67}$) and $GABA_A$ receptors subtypes. Taken together, these results suggest that DCA potentiates pentobarbital-induced sleeping behaviors through the activation of $GABA_A$-ergic systems, and can be useful in the treatment of insomnia.
Woo, Jung-Hoon,Yang, Song-Ju,Yim, Seon-Hee,Hu, Hae-Jin,Shin, Myung-Ju,Oh, Eun-Hee,Kang, Hyun-Woong,Park, Seon-Yang,Chung, Yeun-Jun Korea Genome Organization 2010 Genomics & informatics Vol.8 No.1
To discover genetic markers for autism spectrum disorder (ASD), we previously applied genome-wide BAC array comparative genomic hybridization (array-CGH) to 28 autistic patients and 62 normal controls in Korean population, and identified that chromosomal losses on 8p23.1 and on 17p11.2 are significantly associated with autism. In this study, we developed an 8.5K ASD-specific BAC array covering 27 previously reported ASD-associated CNV loci including ours and examined whether the associations would be replicated in 8 ASD patient cell lines of four different ethnic groups and 10 Korean normal controls. As a result, a CNV-loss on 8p23.1 was found to be significantly more frequent in patients regardless of ethnicity (p<0.0001). This CNV region contains two coding genes, DEFA1 and DEFA3, which are members of DEFENSIN gene family. Two other CNVs on 17p11.2 and Xp22.31 were also distributed differently between ASDs and controls, but not significant (p=0.069 and 0.092, respectively). All the other loci did not show significant association. When these evidences are considered, the association between ASD and CNV of DEFENSIN gene seems worthy of further exploration to elucidate the pathogenesis of ASD. Validation studies with a larger sample size will be required to verify its biological implication.
Anticonvulsant Effect of Artemisia capillaris Herba in Mice
( Tae Seon Woo ),( Seo Young Yoon ),( Ike Campomayor Dela Pena ),( Ji Young Choi ),( Hye Lim Lee ),( Yoon Jung Choi ),( Yong Soo Lee ),( Jong Hoon Ryu ),( Jae Sue Choi ),( Jae Hoon Cheong ) 한국응용약물학회 2011 Biomolecules & Therapeutics(구 응용약물학회지) Vol.19 No.3
In the present study, the anticonvulsant effects of Artemisia capillaris Herba (AC) and its major constituent, esculetin (ECT), were tested and the mechanism studied. Locomotion, Myorelaxation, motor coordination and electroshock seizure experiment were conducted in mice. To identify the anticonvulsant mechanism effect of this drug, chemical-induced seizure in mice and the ionic movement in neuroblastoma cells were also observed. The ethanol extract of AC was orally administered to mice 30 min. prior to testing and ECT was intraperitoneally injected. AC and ECT treatment did not change locomotor activities as well as activities on the rota-rod, which indicates that they did not cause a sedative and myorelaxation effect. AC and ECT treatment increased threshold of convulsion induced by electroshock. AC treatment also inhibited convulsion induced by pentylenetetrazole. In the case of strychnine however, only high dose of AC treatment inhibited convulsion. AC and ECT treatment increased the Cl- influx into the intracellular area in a dose-dependent manner. On the other hand, bicuculline, a GABA antagonist, inhibited the Cl- influx induced by AC and ECT. These results indicate that ECT induces the anticonvulsive effect of AC extract through the GABAergic neuron.
Jae Hoon Woo,Tae-Woo Ha,Jae-Seon Kang,Jin Tae Hong,Ki-Wan Oh 대한생리학회-대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.1
Angelicae Gigantis Radix (AGR, <i>Angelica gigas </i>) has been used for a long time as a traditional folk medicine in Korea and oriental countries. Decursinol angelate (DCA) is structurally isomeric decursin, one of the major components of AGR. This study was performed to confirm whether DCA augments pentobarbital-induced sleeping behaviors via the activation of GABA<sub>A</sub>-ergic systems in animals. Oral administration of DCA (10, 25 and 50 mg/kg) markedly suppressed spontaneous locomotor activity. DCA also prolonged sleeping time, and decreased the sleep latency by pentobarbital (42 mg/kg), in a dose-dependent manner, similar to muscimol, both at the hypnotic (42 mg/kg) and sub-hypnotic (28 mg/kg) dosages. Especially, DCA increased the number of sleeping animals in the sub-hypnotic dosage. DCA (50 mg/kg, p.o.) itself modulated sleep architectures; DCA reduced the counts of sleep/wake cycles. At the same time, DCA increased total sleep time, but not non-rapid eye movement (NREM) and rapid eye movement (REM) sleep. In the molecular experiments. DCA (0.001, 0.01 and 0.1 μg/ml) increased intracellular Cl- influx level in hypothalamic primary cultured neuronal cells of rats. In addition, DCA increased the protein expression of glutamic acid decarboxylase (GAD<sub>65/67</sub>) and GABA<sub>A</sub> receptors subtypes. Taken together, these results suggest that DCA potentiates pentobarbital-induced sleeping behaviors through the activation of GABA<sub>A</sub>-ergic systems, and can be useful in the treatment of insomnia.
조직학적으로 확진한 결핵성 복막염 환자의 복수 Adenosine deaminase ( ADA ) 활성도에 관한 연구
배선우(Seon Woo Bae),진춘조(Choon Jo Jin),심영학(Young Hak Shim),신계철(Kye Chul Shin),권상옥(Sang Ok Kwon),김남동(Nam Dong Kim),차신웅(Shin Woong Cha),이광훈(Kwang Hoon Lee),장우익(Woo Ick Jang) 대한내과학회 1989 대한내과학회지 Vol.36 No.1
N/A Recent investigations have suggested that the measurement of ADA activities in various body fluids is useful in the diagnosis of tuberculous effusion. In this study, ascitic fluid ADA activities were measured in 18 cases of tuberculous peritonitis, 18 cases of malignant ascites and 33 cases of liver cirrhosis with ascites. The results obtained were as fallows: 1) The mean ADA activity in tuberculous peritonitis was 184.96±27.55U/L, in malignant ascites 22.35±3.10U/L and in liver cirrhosis 7.37±0.61U/L. 2) The ADA activity in tuberculous effusion was significantly higher than in the rest of the groups(p<0.001). 3) The malignant effusion show no significant difference with transudative effusion(p>0.05). 4) As a screening test for tuberculous peritonitis, the ADA activities above 50-70U/L had a sensitivity and specificity of 100%. In conclusion, the measurement of ascitic fluid ADA activity may be a simple and reliable screening test for the diagnosis of tuberculous peritonitis.
이현우(Hyun-Woo Lee),양진송(Jin-Song Yang),이재훈(Jae-Hoon Lee),박준형(Jun-Hyong Park),김선엽(Seon-Yeop Kim),최선우(Seon-Woo Choi),이준형(Jun-Hyung Lee),한재현(Jae-Hyeon Han),박찬만(Chan-Marn Park),박정수(Jung-Soo Park) 한국철도학회 2018 한국철도학회 학술발표대회논문집 Vol.2018 No.10
증기의 힘을 동력으로 사용한 이래 영구자석형 동기전동기의 상용화 노력에 이르기까지 철도운전제어기술은 내연기관 및 전기기기와 전력전자 기술의 발달을 힘입어 그 발걸음을 계속 하고 있다. 특히 초창기 철도차량에 적용된 견인전동기는 직류직권전동기를 장착한 철도차량을 운행했지만, 전력전자 기술과 고속 대용량 스위칭 소자의 발달로 개발된 3상농형유도전동기를 장착한 철도차량을 주로 운행하고 있으며 새로운 방식의 철도차량 동력장치 개발 및 기존에 사용 중인 동력장치의 고효율, 경량화, 소형화에 계속해서 힘쓰고 있다. 본 논문에서는 한국철도 역사에 따른 철도차량용 동력장치의 발전과정과 제어방식의 개발동향을 중점적으로 전개·분석하며 향후 발전 가능성과 방향을 함 께 제시하고자 한다.
( Ki Taek Lim ),( Seon Woo Hoon ),( Hyun Mok Son ),( Soo Jung Baik ),( Jang Ho Kim ),( Soung Hoo Jeon ),( Joo Young Park ),( Yun Hoon Choung ),( Chong Su Cho ),( Pill Hoon Choung ),( Jong Hoon Chung ) 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.14
In the design of perfusion bioreactor system for tissue engineering, design parameters including fluid dynamics and mass transport as well as scaffolds should be optimized to maximize cell growth. Critical elements in bioreactor systems based on cells and scaffolds include cell seeding, the nutrient and gas supply to cells, and mechanical stimuli. In particular, perfusion flow systems have been shown to enhance cell growth, differentiation, and tissue formation as well as provide for the appropriate and necessary mass transfer of nutrients, gases, metabolites, and regulatory molecules. In this paper, we reviewed the biomechanical models in relation with fluid dynamics, mass transport, and shear stress with scaffolds. Therefore, obtaining a proper oxygen supply, high cell density, and an uniform cell distribution in a three-dimensional growth support are important. Both experiments and quantitative mathematical models of cell culture in perfusion bioreactors are needed to better understand physical, mechanical, and biochemical conditions and for the design of a suitable bioreactor system the mathematical describes the oxygen mass transfer and cell density within a three-dimensional scaffold. This review focuses on biomechanical models in relation with fluid dynamics, mass transport, and shear stress to cells cultured, and cell growth in perfusion bioreactors for tissue engineering.
Feature Article : Novel Perfusion Bioreactor Systems for Tissue Engineering
( Ki Taek Lim ),( Chong Su Cho ),( Yun Hoon Choung ),( Jang Ho Kim ),( Hyun Mok Son ),( Hoon Seon Woo ),( Soo Jung Baik ),( Soung Hoo Jeon ),( Joo Young Park ),( Pill Hoon Choung ),( Jong Hoon Chung ) 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.1
Bioreactor systems that use a new aeration concept have recently been introduced. Bioreactors can assist in the development of new tissues in vitro by providing appropriate stimuli to the cell cultures. The design of bioreactors for tissue engineering is very complex and is often tailored to individual cell- engineered-tissue products. Critical elements in bioreactor systems based on cells and scaffolds include cell seeding, the nutrient and gas supply to cells, and mechanical stimuli. Flow perfusion systems have been shown to enhance cell growth, differentiation, and tissue formation as well as provide for the appropriate and necessary mass transfer of nutrients, gases, metabolites, and regulatory molecules. The beneficial effects of fluid flow induced convective transport and shear stress have been exploited in various types of bioreactors because fluid flow-through cell and tissue engineered constructs increase nutrient transport. These systems are called flow-perfusion bioreactors. In this paper, we review the characteristics of bioreactor systems used for cell culture in tissue engineering, and provide a schematic design for a novel bioreactor system that incorporates the elements we deem critical for such systems.