소수성 촉매를 이용한 수소동위원소 교환 반응 측정을 위한 순환 반응기

최희주,이한수,안도희,강희석,김광락 한국공업화학회 2002 응용화학 Vol.6 No.2

The catalytic rate constants of hydrophobic catalysts developed for the removal of tritium mainly generated in the pressurized heavy water reactors should be measured. A recycle reactor was designed and built for characterzing the long-term behavior of the catalyst performance at the various temperatures and gas velocities.The catalytic rate constants were measured at the apparent gas velocity of 0.5 m/s and the temperature of 60℃. The initial catalytic constant was 8.44×10^-4mol/g.sec. This value is much greater than that considered in the design of Wolsong Tritium Removal Facility.

인슐린 비의존형 당뇨병 환자에서 혈장 Endothelin-1농도의 변화

신양수,조희충,김원식,국기용,김용화,정종훈,문철웅,배학연,양성훈 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

Endothelin(ET) is a 21-residue peptide originally isolated from the cultured porcine endothelial cells. There are at least three genes for endothelin:endothelin-1(ET-1), endothelin-2(ET-2), and endothelin-3(ET-3). Endothelins are present in various human biological fluids including plasma, urine, breast milk, and saliva and have been found elevated plasma ET concentrations in patients with diabetes mellitus, Patients undergoing maintenance hemodialysis due to chronic renal failure, patients with acute myocardial infarction, and patients with subarachnoid hemorrhage. Endothelial cell damage is suspected to occur in diabetic patients and may be one important cause of angiopathy, a major complication in diabetes mellitus. The elevation of ET in diabetic patients may be a marker of, and further exacerbate, their vascular disease. We measured the levels of ET-1 in plasma of 50 patients with non-insulin dependent diabetes mellitus(NIDDM) and 25 normal subjects by radioimmunoassay. The plasma ET-1 concentration (mean±S.D.) in NIDDM was 6.461 A2.510 f㏖/ ㎖, and was significantly higher than in normal subjects (4.567±1.155f㏖/㎖) (P<0.05). The plasma ET-1 concentration (mean±S.D.) in diabetic retinopathy group( 7.15±2.454 f㏖/ml) was significantly elevated than those in otherwise uncomplicated groups (5.348±2.390 f㏖/㎖)(P<0.01). The correlation between any clinical parameters and plasma ET-1 levels in NIDDM was not significant, In conclusion, this study suggest that the elevated levels of ET-1 in diabetic patients may be play a important role in the pathogenesis of diabetic complications.

New Zealand White 토끼의 생식세포 및 체세포 분열에 의한 염색체 분석

신선희,김희수,최영현,이원호 부산대학교 유전공학연구소 2001 분자생물학 연구보 Vol.17 No.-

토끼(New Zealand White rabbit)의 감수분열 및 유사분령상을 통해 그 염색체적 특징을 조사하였다. 감수분열 염색체의 표본 작성은 공기건조법을 다소 변형하여 사용하였고, 유사분열 중기상의 핵형분석에는 G-분염법을 이용하여 다음과 같은 결과를 얻었다. 1. 제1감수분열의 접합기와 태사기의 염색소립과 sex vesicles 및 동원체가 뚜렷하게 구별되었다. 태사기의 중기에서부터 후기 과정이 진행되는 동안에 2가염색체에서 측면으로 돌출한 머리카락 모양의 돌기들이 관찰되어졌고, 이 돌기들은 염색체가 lampbrush 염색체 구조의 모양이 되도록 해주었다. 이동기의 염색체들은 키아즈마의 수와 위치에 따라 분류가 가능하였다. 제1 감수분열 전기의 후반부 과정과 중기Ⅰ 시기에는 2가염색체로 된 21개의 상염색체와 1개의 말단결합으로 연결된 X-Y 염색체를 관찰할 수 있었다. 2. New Zealand White rabbit의 생식세포에서 관찰된 2가 염색체의 형태는 1CH, 1TAl 및 2TA 2가염색체 유형이 대부분을 이루었다. New Zealand White rabbit에서의 평균 키아즈마 빈도는 약 30.2로 나타났으며, 키아즈마 빈도가 복사기, 이동기 및 중기Ⅰ로 분열이 진행됨에 따라 다소 감소하는 경향이 있었다. 3. New Zealand White rabbit의 핵형분석에 의하면 염색체 수는 44개(2n=44)로서, 8쌍의 중부염색체, 9쌍의 차중부염색체, 4쌍의 단부염색체로 된 21쌍의 상염색체와 중부염색체인 1개의 X 염색체 및 단부염색체인 1개의 Y 염색체로 구성되어 있음을 알 수 있었다. Chromosomal characteristics of New Zealand White rabbit was studied at meiosis and mitosis. The meiotic chromosomal preparations were made with the modified air-drying method and karyotype analysis was performed with the G-banding technique, using isolated mitotic metaphase chromosomes of the New Zealand White rabbit. Chromosomes, sex vesicles and centromeres could be could be classified in the zygotene and the pachytene of the meiosis Ⅰ. The hair-like processes projecting laterally from the axes of bivalent chromosomes at the mid-late pachytene were basis of the numbers and the locations of chiasma in the diakinesis. Twenty-one autosomal bivalents and a single unequal terminally associated X-Y bivalent were observed during the late prophase and the metaphase of the meiosis Ⅰ. Most of the bivalent types observed in the New Zealand White rabbit spermatocytes were 1CH, 1TAl, and 2TA bivalents. The mean chiasma frequency (CE) of the male New Zealand White rabbit was 30.2, and it was found that the CF value tended to decrease through the diakinesis and the metaphase Ⅰ. The karyotype of the New Zealand White rabbit was a male chromosome number of 44(2n=44), comprising 8 pairs of metacentric, 9 pairs of submetacentric, 4 pairs of acrocentric autosomes, metacentric X chromosome and acrocentric Y chromosome.

크래시된 이미지와 딥 클러스터링을 통한 크래시 분류 개선

김요한 ( Yo-han Kim ),이상준 ( Sang-jun Lee ) 한국정보처리학회 2019 한국정보처리학회 학술대회논문집 Vol.26 No.2

소프트웨어 크래시 분류를 개선하기 위해 호출 스택 정보를 기반한 많은 연구들이 있다. 본 연구에서는 크래시 직전 이미지를 수집하여, 기존 호출 스택 기반의 분류에서 발생하는 문제를 개선하고자 한다. 또한 이미지 자체의 직관성으로 개발자뿐만 아니라 개발 지식이 없는 실무자도 크래시 정보를 활용할 수 있고, 문제 해결을 위한 재현 루트 파악, 위변조 여부와 같은 추가 정보를 확인할 수 있을 것으로 기대한다. 비지도 학습 기반인 딥러닝 클러스터링 N2D 알고리즘을 통하여 이미지를 자동 분류하고 순위화하는 시스템을 구축하여, 특정 소프트웨어에 특화되지 않고 다양한 소프트웨어의 크래시 이미지 자동 분류에 기여할 수 있을 것으로 기대한다.

Effect of Salinity, Temperature, and Glucose on the Production of Vibrio vulnificus Hemolysin

Kim, Hyun-Soo,Shin, Sung-Heui,Park, Hae-Ryoung,Lee, Shee-Eun,Kim, Choon-Mee,Kim, Soo-Young,Kim, Young-Ran,Lee, Hyun-Chul,Chung, Sun-Sik,Rhee, Joon-Haeng The Korean Society for Microbiology 2002 Journal of Bacteriology and Virology Vol.32 No.4

Among the exotoxins produced by V. vulnificus, hemolysin (HS) has been reported to be the most potent one. To investigate the factors up- or down-regulating HS production in the context of pathogenesis, we observed the effects of salinity or/and temperature shifting, glucose, and acidic pH on the production of HS by V. vulnificus C7184 strain in vitro. Significantly more HS was produced when V. vulnificus was cultured in 0.9% salinity and $37^{\circ}C$ than in 2.5% and $25^{\circ}C$. When the culture condition reflecting natural habitat of V. vulnificus (2.5% salinity and $25^{\circ}C$) was changed into that reflecting human body (0.9% salinity and $37^{\circ}C$), 2.5 fold or more HS was produced than in the V. vulnificus being cultured continuously in 0.9% NaCl at $37^{\circ}C$. This result suggests that V. vulnificus somehow recognizes the shifting in salinity and temperature and stimulate HS production. Glucose addition in the culture medium resulted in a dose-dependent decrease in the HS production. Glucose itself and acidic pH resulting from its metabolism both appeared to inhibit the HS production. Glucose in itself had more dominant role in suppressing the HS production than the lowered pH accompanying the metabolism of glucose. This result suggests that HS production is down-regulated in the presence of glucose and under environmental acidic pH.

Inhibitory effects of natural plants of Jeju Island on elastase and MMP-1 expression.

Kim, Young Heui,Kim, Ki Soo,Han, Chang Sung,Yang, Hong Chul,Park, Sun Hee,Ko, Kang Ii,Lee, Soo Hee,Kim, Ki Ho,Lee, Nam Ho,Kim, Jung Mi,Son, Kyung-Hun Society of Cosmetic Chemists 2007 Journal of cosmetic science Vol.58 No.1

<P>In order to search for new active cosmetic ingredients of natural origin, we screened about 60 plants collected from Jeju Island, which is located in the southernmost part of the Republic of Korea. We investigated their free radical scavenging activity, elastase inhibition activity, and reduction of MMP-1 mRNA expression for the development of anti-aging ingredients as raw materials for use in cosmetics. In the free radical scavenging capacity assay, 12 extracts, including Typha orientalis (seed) and Torreya nucifera (leaf), showed significant free radical scavenging activity (up to SC(50)<30 microg/ml). Among these extracts, Nymphaea tetragona (rhizome) extract showed the highest free radical scavenging activity (SC(50)=4.7 microg/ml). In the anti-elastase inhibition assay, seven extracts, including Typha orientalis (seed) and Persicaria hydropiper (whole plant), showed high inhibitory activity (>50% at 100 mug/ml). Among these extracts, Persicaria hydropiper (whole plant) extract showed the highest elastase inhibition activity (IC(50) = 46.7 mug/ml). In the MMP-1 expression assay using RT-PCR, Typha orientalis (seed), Pyrrosia hastata (root), and Capsicum annum (whole plant) showed slightly lower inhibition activity than EGCG, which was used as a control. Furthermore, four extracts, including Persicaria hydropiper (whole plant), Filipendula glaberrima (root), Nymphaea tetragona (root), and Camellia japonica (leaf), completely inhibited the expression of MMP-1 in human fibroblast cells. The results showed that four of the 60 plant extracts may hold potential for use as natural active ingredients for anti-aging cosmetics.</P>

Endogenous Retroviral Elements and Neuropsychiatric Diseases

Kim, Heui-Soo 가톨릭의과학연구원 2000 가톨릭 의과학연구원 국제학술대회 Vol.4 No.-

The hypothesis that the predisposing gene has the structure of a retrovirus or retrotransposon (Grow, 1984) explains some aspects of psychosis (eg episodicity), and is susceptible to investigation through molecular homologies. We have employed a cimbination of a PCR search based upon known sequences and phylogenetic analysis of these sequences in Home sapiens and hominoid primates to investigate this hypothesis. We have identidied three classes of element that have been subject to recent change in the course of priate evolution and that include members that are specific to the human genome: (1) the SINE-R. C2 element that has been associated with Fukuyama-type muscular dystrophy (Kim et al, 1999 a,b,c), and long terminal repeat (LTR) structures associated with the human endogenous retroviruses(HERVs) in the (2) HERV-K (kim et al, 1999 d)and (3) HERV-W classes (Kim et al,1999 e f). Each of these classes has the capacity to activity adjacent genes and includes members that have been subject to recent transposition in the human genome. We have investigated elements in these classes that are preset within the region of Xq21.3/Yp region of homology that was generated by a transposition from the X to the Y chromosome that occured after the separation of the chimpanzee and Home sapiens lineages. These elements are of interest in relation to the hypothsis that a gene for cerebral asymmetry is present within this region, and is associated with variation that is relevant to predisposition to neuropsychiatric diseases (crow, 1999).

Molecular Phylogeny of Endogenous Retrovirus HERV-F Family in Japanese and Rhesus Monkeys

Kim, Heui-Soo,Jeon, Seung Heui,Yi, Joo-Mi,Kim, Tae-Hyeong,Kim, Myung-Sook,Hyun, Byung-Hwa,Takenaka, Osamu 한국유전학회 2002 Genes & Genomics Vol.24 No.2

A new human endogenous retroviral family (HERV-F) has recently been identified from the human chromosome 7q31.1-q31.3 that was identical to the XA34 cDNA clone isolated from a human glioma cDNA library with an ERV-9 probe. The current study investigated pol fragments of the HERV-F family from Japanese and rhesus monkeys and compared them with those of the HERV-F (Hu-XA34) family. Fourteen pol fragments of the HERV-F family were detected from the monkeys, which showed a 78.7-95.4% sequence similarity with those of HERV-F (Hu-XA34). Clones FJM-1, FJM-7, FJM-14, and FJM-15 from the Japanese monkey and FRH-1 and FRH-4 from the rhesus monkey exhibited no disruption due to point mutation or insertions/deletions. The ratio of synonymous to non-synonymous substitutions indicated that negative selective pressure was acting on these clones. Therefore, the pol gene sequences could be associated with an active provirus in the monkey genomes. A phylogenetic analysis of the pol fragments from humans and monkeys using the neighbor joining and maximum parsimony methods showed six groups, indicating that either the HERV-F family was amplified at least six times after its original integration into the monkey genome or the occurrence of independent integration events during primate evolution.

Genome based quantification of VHSV in multiple organs of infected olive flounder (Paralichthys olivaceus) using real-time PCR

Hyunsu Kim,Kyung‑Wan Baek,고은지,Nguyen Thanh Luan,Yunjin Lim,Heyong Jin Roh,Nameun Kim,Do‑Hyung Kim,Ahran Kim,Yung Hyun Choi,Suhkmann Kim,Heui‑Soo Kim,Mee Sun Ock,차희재 한국유전학회 2020 Genes & Genomics Vol.42 No.7

Background Viral hemorrhagic septicemia (VHS) is a serious viral disease that infects the olive flounder in South Korea. The Korean aquaculture industry experienced an economic loss caused by the high infectivity and mortality. Objective This study aimed to evaluate the infection density of VHSV in various organs of the olive flounder including spleen, liver, kidney, stomach, esophagus, intestine, gill, muscle, heart, and brain. Olive flounders were collected from a local fish farm and injected subcutaneously with 106 PFU/fish. Methods Each 15 fish were sampled at 0, 3, and 7 days post challenge (dpc), respectively, to perform quantitative analysis of VHSV using SYBR-green based real-time PCR in various tissues including spleen, liver, head-kidney, body-kidney, muscle, esophagus, stomach, intestine, gill, and brain. Results Organs infected with VHSV were obtained after 3 and 7 days. Each organs were examined for viral infection using real-time PCR. The data obtained from this experiment revealed copy numbers higher than 10 copies per 100 ng cDNA in the spleen (15.26 ± 3.11 copies/100 ng of cDNA), muscle (11.24 ± 2.25 copies), and gill (14.23 ± 6.26 copies), but lower in liver, head-kidney, body-kidney, esophagus, brain and stomach. Conclusion The present study, together with previous data, demonstrated that the gill, spleen, and muscle are the major target organs of VHSV in olive flounder. Therefore, central monitoring of spleen, gill and muscle should be considered and might be necessary if anti-VHSV treatment is to be successful in infected olive flounder.

Effect of Salinity, Temperature, and Glucose on the Production of Vibrio vulnificus Hemolysin

Hyun-Soo Kim,Sung-Heui Shin,Hae-Ryoung Park,Shee-Eun Lee,Choon-Mee Kim,Soo-Young Kim,Young-Ran Kim,Hyun-Chul Lee,Sun-Sik Chung,Joon-Haeng Rhee 대한미생물학회 2002 Journal of Bacteriology and Virology Vol.32 No.4