다기능 미세 단백질 사이모신 베타 4 (Thymosin β4)

차희재 한국생화학회 2009 생화학분자생물학회 소식 Vol.29 No.2

Effect of irradiation on cytokine secretion and nitric oxide production by inflammatory macrophages

차희재,최영현,임상욱,고은지,강윤정,백경완,옥미선,송경섭,강혜주,금영삼,현진원,권택규,남선영 한국유전학회 2016 Genes & Genomics Vol.38 No.8

This study explored the effects of low-dose and high-dose irradiation on inflammatory macrophage cells, specifically inflammatory cytokine secretion and nitric oxide (NO) production after irradiation. To elucidate the effect of irradiation on active and inactive macrophages, we exposed LPS-treated or untreated murine monocyte/macrophage RAW 264.7 cell lines to low-dose to high-dose radiation (0.01–10 Gy).Weanalyzed the effects of irradiation onRAW 264.7 cell proliferation byMTT assays and analyzed cytokine secretion and NO production related to inflammation by ELISA assays. Low-to-high doses of radiation did not significantly affect the proliferation of LPS-treated or untreated RAW 264.7 cells. Pro-inflammatory cytokine IL-1ß was generally increased in RAW 264.7 cells at 3 days after radiation. Especially, IL-1ß was significantly increased in only high dose-irradiation (2 and 10 Gy irradiation) groups in LPSuntreated RAW264.7 cells but increased in both lowand high dose-irradiation groups (0.01–10 Gy) in LPS-treated RAW 264.7 cells at 3 days after irradiation.Whereas, the expression of IL-1ß was prolonged in high-dose irradiation group at 5 days after irradiation. The production of anti-inflammatory cytokine IL-10 did not change significantly at 3 days after radiation but was significantly reduced at 5 days after 10 Gy radiation. The effect of irradiation on the secretion of IL-1ß and IL-10 was not significantly different between RAW264.7 cells treated or not treated with LPS. The effect of irradiation on NO secretion by RAW 264.7 cells showed a specific pattern. NO was produced after low-dose irradiation but reduced in a high-dose irradiation group at 3 days after irradiation. However, NO production was not changed after low-dose irradiation and reduced at 5 days after high-dose irradiation. These results showed that irradiation affected the inflammatory systemand regulatedNOproduction in both activated and inactivated macrophages through different regulation mechanisms, depending on irradiation dose.

최신 Single Nucleotide Polymorphism (SNP) 분석법의 원리 및 유전자 연구 응용법

차희재 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.3

This mini review describes the principles, protocols, and applications of three commercially available Single Nucleotide Polymorphism (SNP) genotyping platforms, the TaqMan® SNP Genotyping Assay, SNPlexTM Genotyping System and Gene Chip Assay. These technologies meet the requirements of multiple SNP applications in genetics research including diseases based on genetic background. This article also describes a set of SNP selection guide and useful web sites for SNP informations. Overall, the TaqMan assay format is suitable for low- to mid-throughput applications in which a high assay conversion rate, simple assay workflow, and low cost of automation are desirable. The SNPlex Genotyping System, on the other hand, is well suited for SNP applications in which throughput and cost-efficiency are essential, e.g., applications requiring either the testing of large numbers of SNPs and samples, or the flexibility to select various SNP subsets. Gene Chip Assay is powerful tool to analyze whole SNPs and has benefit to find new SNPs involved diseases or phenotype but has a defect that it is costly. In the conclusion, this article suggests the appropriate methods for the desired experiments by comparing with these three SNPs technologies.

Preventive and Therapeutic Effects of Anisakis simplex Larval Protein in a Mouse Model of Crohn’s Disease

차희재,옥미선 고신대학교(의대) 고신대학교 의과대학 학술지 2013 고신대학교 의과대학 학술지 Vol.28 No.2

Objectives: Some helminths have been known to have a treatment effect in inflammatory bowel diseases, including Crohn’s disease (CD); however, live parasite therapy can cause unwanted side effects. To develop a safe therapeutic, we investigated the preventive or therapeutic potential of proteins from the third stage larva of A. simplex in a mouse model. We also analyzed the cytokine profile from splenic and mesenteric lymph node lymphocytes to elucidate the underlying immunological mechanism. Objectives: Some helminths have been known to have a treatment effect in inflammatory bowel diseases, including Crohn’s disease (CD); however, live parasite therapy can cause unwanted side effects. To develop a safe therapeutic, we investigated the preventive or therapeutic potential of proteins from the third stage larva of A. simplex in a mouse model. We also analyzed the cytokine profile from splenic and mesenteric lymph node lymphocytes to elucidate the underlying immunological mechanism. Methods: CD was induced in mice with DSS, and the effect of an A. simplex larval protein on CD was assessed. A change in body weight and DAI (disease activity index) were observed in mice. The expression levels of cytokines from mesenteric lymph nodes (MLN) compared to splenic lymphocytes were measured with ELISA. Results: Peritoneal administration of preventive and therapeutic A. simplex larval proteins attenuated DSS-induced CD by a reduction of the DAI and weight loss. A shortening of colon length was more definitely observed in the therapeutic group than in the preventive group. The cytokine expression levels were more obvious in lymphocytes from mesenteric lymph nodes than from splenic lymphocytes. Conclusions: Taken together, these results suggest that A. simplex proteins can change cytokine profiles and may have a preventive effect in DSS-induced CD mice.

Anisakidosis in Korea; Changes Over the Last Decade

차희재,Mee-SunOCK 고신대학교(의대) 고신대학교 의과대학 학술지 2012 고신대학교 의과대학 학술지 Vol.27 No.2

Anisakidosis is caused by Anisakis simplex and other anisakids larvae parasitizing marine fish and cephalopods. A lot of case reports about anisakidosis have been published in Korea because of raw fish eating habits. Recently seafood consumption has continued to increase due to health concerns and thus, it increases the risk for infectious diseases including anisakidosis. The aim of this review is to analyze the clinical and epidemiological characteristics of anisakidosis during the last 10 years in Korea, based on the case reports published from 2000 to 2010. The incidence age was changed from 30s and 40s to 50s. The young generation was considered to consume seafood in various ways, including raw fish as well. The most noticeable change was the appearance of Anisakis allergy patients over the last decade. The patients showed abdominal pain, urticaria after eating sea food. It reaffirmed that anisakid infection induces not only gastric and intestinal anisakidosis but also cause allergic reaction. Anisakid should be considered as a possible causative food allergen provoking allergic responses after eating raw fish.

The Roles of Human Endogenous Retroviruses (HERVs) in Inflammation

고은지,차희재 고신대학교(의대) 고신대학교 의과대학 학술지 2021 고신대학교 의과대학 학술지 Vol.36 No.2

Human endogenous retroviruses (HERVs) are ancient, currently inactive, and non-infectious due to recombination, deletions, and mutations in the host genome. However, HERV-derived elements are involved in physiological phenomena including inflammatory response. In recent studies, HERV-derived elements were involved directly in various inflammatory diseases including autoimmune diseases such as rheumatoid arthritis (RA), multiple sclerosis, amyotrophic lateral sclerosis (ALS), and Sjogren’s syndrome. Regarding the involvement of HERV-derived elements in inflammation, two possible mechanisms have been proposed. First, HERV-derived elements cause nonspecific innate immune processes. Second, HERV-derived RNA or proteins might stimulate selective signaling mechanisms. However, it is unknown how silent HERV elements are activated in the inflammatory response and what factors and signaling mechanisms are involved with HERV-derived elements. In this review, we introduce HERV-related autoimmune diseases and propose the possible action mechanisms of HERV-derived elements in the inflammatory response at the molecular level.

Interactions between human endogenous and exogenous retroviruses

김희수,차희재,옥미선 한국유전학회 2017 Genes & Genomics Vol.39 No.9

Retrovirus genes have become inserted into the human genome for more than one million years. These retroviruses are now inactivated due to mutation, such as deletions or nonsense mutations. After mutation, retroviruses eventually become fixed in the genome in the endogenous form and exist as traces of ancient viruses. These retroviruses are called human endogenous retroviruses (HERVs). HERVs cannot make fully active viruses, but a number of viral proteins (or even virus particles) are expressed under various conditions. By comparison with ERVs, some exogenous retroviruses are still infectious and cause serious diseases threatening human life. Recent studies have shown that some elements of HERVs are closely related to other exogenous retroviruses, including human immunodeficiency virus (HIV). This review will describe the regulation and interaction between HERVs and other active viral infections. In addition, we introduce the development of vaccines and therapeutic agents against these viral infections through the use of HERV elements.

Spermidine Protects against Oxidative Stress in Inflammation Models Using Macrophages and Zebrafish

정진우,차희재,한민호,황수정,이대성,유종수,최일환,김석만,김희수,김기영,홍수현,박철,이효종,최영현 한국응용약물학회 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.2

Spermidine is a naturally occurring polyamine compound that has recently emerged with anti-aging properties and suppresses inflammation and oxidation. However, its mechanisms of action on anti-inflammatory and antioxidant effects have not been fully elucidated. In this study, the potential of spermidine for reducing pro-inflammatory and oxidative effects in lipopolysaccharide (LPS)-stimulated macrophages and zebrafish was explored. Our data indicate that spermidine significantly inhibited the production of pro-inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2), and cytokines including tumor necrosis factor-α and interleukin-1β in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects of spermidine accompanied by a marked suppression in their regulatory gene expression at the transcription levels. Spermidine also attenuated the nuclear translocation of NF-κB p65 subunit and reduced LPS-induced intracellular accumulation of reactive oxygen species (ROS) in RAW 264.7 macrophages. Moreover, spermidine prevented the LPS-induced NO production and ROS accumulation in zebrafish larvae and was found to be associated with a diminished recruitment of neutrophils and macrophages. Although more work is needed to fully understand the critical role of spermidine on the inhibition of inflammation-associated migration of immune cells, our findings clearly demonstrate that spermidine may be a potential therapeutic intervention for the treatment of inflammatory and oxidative disorders.

Ginsenoside Rh_1_ 및 Rh_2_ 의 HT1080 세포 침윤억제 작용에 관한 연구 ( Anti-invasive Activity of Ginsenoside Rh_1_ and Rh_2_ in the HT1080 Cells )

박문택,차희재,정주원,이호영,김신일,백남인,김옥희,김규원 고려인삼학회 1998 Journal of Ginseng Research Vol.22 No.3

폐경기증상과 자궁적출술 및 양측 난소제거술이 골밀도에 미치는 영향

오영림,옥미선,차희재,김흥열 대한골다공증학회 2009 Osteoporosis and Sarcopenia Vol.7 No.2

목적: 폐경기 전후의 여성을 대상으로 폐경기 증상이 있는 여성과 없는 여성의 골밀도를 비교하고, 전자궁적출술 및 양측부속기제거술을 시행한 폐경기 여성과 자연적 폐경기 여성의 골밀도를 비교하였으며, 그 외 산과력, 체중 등이 골밀도에 미치는 영향을 조사하여 골다공증의 예방과 치료에 도움을 얻고자 이 연구를 시행하였다. 방법: 2008년 1월 1일부터 2008년 8월 31일까지 고신대학교 의과대학 부속병원 산부인과 외래를 방문한 170명의 환자 중 전자궁적출술 및 양측 난소제거술을 시행한 70명, 전자궁적출술만 시행한 25명, 자연적 폐경기 여성 75명을 대상으로 척추 및 대퇴골 경부, 전자 및 Ward씨 삼각부의 골밀도를 측정하여 다음과 같은 결과를 얻었다. 결과: 폐경기 증상이 있는 여성과 폐경기 증상이 없는 여성의 골밀도를 비교했을 때, 증상이 없는 여성이 증상이 있는 여성에서 보다 골밀도가 높았으며 대퇴골 경부 및 전자의 골밀도 측정치는 통계적 유의성이 있었으나(P<0.05), 척추 및 대퇴골의 Ward씨 삼각부에서의 골밀도는 유의성이 없었다. 자연적 폐경기 여성과 전자궁적출술을 시행한 여성의 골밀도를 측정한 결과 척추 및 대퇴골경부, 전자및 Ward씨 삼각부의 골밀도가 전자궁적출술을 시행한 여성에서 통계적으로 유의하게 낮았다(P<0.01) 단순 전자궁적출술을 시행한 여성과, 양측 난소를 제거한 여성의 골밀도를 비교한 결과 단순 전자궁적출술만을 시행한 경우가 전자궁적출술 및 양측난소를 제거한 여성보다 골밀도 측정치가 유의성 있게 낮았다(P<0.01). 결론: 수술로 인한 폐경기 여성에서 자연적 폐경기 여성에 비해 골밀도가 유의성있게 저하되어 골다공증으로 인한 골절의 위험성이 높으므로 조기 진단과 치료가 요망된다.