Induction of nucleolin translocation by acharan sulfate in A549 human lung adenocarcinoma

Joo, Eun Ji,Yang, Hui,Park, Youmie,Park, Nam Young,Toida, Toshihiko,Linhardt, Robert J.,Kim, Yeong Shik Wiley Subscription Services, Inc., A Wiley Company 2010 Journal of cellular biochemistry Vol.110 No.5

<P>Acharan sulfate (AS), isolated from the giant African snail Achatina fulica, is a novel glycosaminoglycan, consisting primarily of the repeating disaccharide structure α-D-N-acetylglucosaminyl (1 → 4) 2-sulfoiduronic acid. AS shows anti-tumor activity in vitro and in vivo. Despite this activity, AS is only weakly cytotoxic towards cancer cells. We examine the interactions between AS and cell-surface proteins in an effort to explain this anti-tumor activity. Using flow cytometry and affinity column chromatography, we confirm that AS has strong affinity to specific cell-surface proteins including nucleolin (NL) in A549 human lung adenocarcinomas. Surprisingly, we found the translocation of NL from nucleus to cytoplasm under the stimulation of AS (100 µg/ml) in vitro. Also, as NL exits the nucleus, the levels of growth factors such as bFGF and signaling cascade proteins, such as p38, p53, and pERK, are altered. These results suggest that the communication between AS and NL plays a critical role on signal transduction in tumor inhibition. J. Cell. Biochem. 110: 1272–1278, 2010. Published 2010 Wiley-Liss, Inc.</P>

운동신경원성 질환을 동반한 전두측두엽치매의 임상양상과 신경심리소견

박기정,정용,김은주,진주희,강수진,나덕렬 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.1

Backgrounds:Frontotemporal dementia (FTD) is rarely associated with motor neuron disease (MND). This comorbidity (FTD-MND), a subtype of FTD, results in progressive dementia and muscle weakness. Among the few available reports of series of patients, however, there have been controversies about the clinical course of FTD-MND. This study, the first report of a series of FTD-MND patients in Korea, investigated demographic and clinical features, clinical course, and neuropsychological findings of nine patients with FTD-MND. Methods:Nine FTD-MND patients (2 men and 7 women with mean age 55.6±7.2 years) were selected among 45 FTD patients who met the FTD criteria proposed by the Lund and Manchester Groups. Their clinical and neuropsychological findings were analyzed retrospectively. Results:Mean age of onset was 54.3±8.0 years. The time interval from onset to death was 27±11.3 months. Presenting symptoms were personality change, hyperphagia, nonfluent speech or motor weakness. In most cases (8/9), dementia preceded the muscle weakness;in only one patient the muscle weakness preceded the cognitive decline. Regarding the muscle weakness, bulbar symptoms occurred earlier than limb weakness in four patients;bulbar and limb weakness occurred simultaneously in another four patients;in the remaining one patient limb weakness preceded bulbar weakness. Neuropsychologic tests showed no specific patterns but diffuse dysfunction in all cognitive domains. Conclusions:Our study suggests that FTD-MND predominantly affects the bulbar muscles rather than the limb muscles in early stage and symptoms related to MND occurs later than those related to FTD. The time from diagnosis to death was 10.6±6.5 months, suggesting that FTD-MND is a rapidly progressive disease. This rapid clinical course may account for our neuropsychological findings that showed general cognitive deficits father than predominant frontal dysfunctions.

Cu-Al-Ni계 초탄성 합금의 변태특성 및 변형거동에 미치는 미세조직의 영향

박정수,김경훈,은상원,조무현,장우양,양권승,강조원 조선대학교 생산기술연구소 2004 生産技術硏究 Vol.26 No.2

The effect of microstructure on transformation characteristics and deformation behavior in Cu-Al-Ni based superelastic alloy has been studied in alloy by using metallography, calorimetry, compression test and XRD. When the alloy betatized at the elevated temperature above 600℃, the r₂ phase completely dissolved into the matrix. The β₁' or r₁' martensites from β₁ patent phase was transformed in as-cast state but the parent phase stabilized by betatizing at 600℃. With increasing betatizing temperatures above 600℃. both₁' and r₁' martensites again transformed from β₁ parent phase and the heat flow of r₁' martensite was higher than that of β₁' martensite for reverse transformation. The compressed specimen, showing pseudoelastic behavior, was completely recovered upon unloading. However, the stress-strain curves for compression test were different by betatizing temperature; the stress-strain curves of the specimen betatized at 600℃ and 700℃ were linear but those of the specimen betatized at 800℃ and 900℃ were non-linear. The r₁' martensite was retained after compression test and the volume fraction of that increased with increasing betatizing temperature and strain.

CNTF에 의한 신경세포의 재생에 관여하는 신호전달체계에 관한 연구

은수용,조철만,이지연,박정욱,강병학,엄의영,안상미 국립보건연구원 1998 국립보건원보 Vol.35 No.-

IMx Glycated Hemoglobin측정법에 관한 연구

황성준,박종성,이승관,이창규,류정록,변대훈,박은병 高麗大學校 倂設 保健大學 保健科學硏究所 1994 保健科學論集 Vol.20 No.1

Percent glycated hemoglobin(%GHb) is an indirect measure of the mean blood glucose level in an individual over the previous two to three months. A fully automated assay for quantitating %GHb in human whole blood has been developed using the Abbot IMx R analyzer. The assay is accomplished by specific solution phase tagging of glycated hemoglobin by a soluble polyanionic affinity reagent, and subsequent capture of the anionic complex by a cationic solid phase matrix. Both glycated hemoglobin(GHb) and total hemoglobin(Hb) are quantitated by measuring quenching of the fluorescence from an added dye by home. A correlation study was conducted accross IMx R instruments with fully automated HPLC (Hi-Auto A_(IC TM), Kyoto Daiiclii co.) as the reference assay. The correlation statistics obtained for 20 diabetic specimens were: IMx=0.84 HPLC+1.75, correlation coefficient= 0.969. Studies from linear relationship statistics had showed that there ere no evidence for difference between two methods. IMx glycated hemoglobin assay was don-sidered to be compatible with HPLC method.

화학물질에 의한 폐염 1예 보고

이성우,은충기,김재하,박수성 중앙대부설한국의과학연구소 1974 한국의과학 Vol.6 No.6

내시경적 점막절제술 후 발생한 위천공의 성공적인 보존적 치료 1예

양정채,박은하,이준행,이풍렬,김재준,백승운,이종철 대한내과학회 2004 대한내과학회지 Vol.66 No.5

위장관 천공은 내시경적 점막절제술 후 발생할 수 있는 중대한 합병증으로 현재까지 이에 대한 치료 방법은 정립되지 않은 상태이다. 본 증례는 점막층에 국한된 위선암에 대한 내시경적 점막절제술 후 위천공이 발생한 경우로서 아주 작은 미세천공(microperforation)이었고, 최근 시도되고 있는 내시경적 클립 시술의 적응이 되지 못했다. 하지만 임상적인 복막염 발생 및 진행의 징후가 보이지 않아 금식, 광범위 항생제 투여 등의 보존적 요법으로 치료를 시행하였고, 시술 일주일 후 복막염의 발생이나 진행 없이 호전되어 퇴원하였다. 본 증례는 내시경적 점막절제술 후 발생한 미세 천공의 제한적인 경우에 한하여 수술이나 내시경적 치료를 하지 않고 보존적 요법만으로 성공적인 치료를 시행한 예로 문헌고찰과 함께 보고하는 바이다. The serious complication after endoscopic mucosal resection (EMR) was bleeding and perforation. Most of bleedings could be controlled by endoscopic procedure. However, the strategy of treatment for perforation was not established. A 60- year-old man was admitted to our center for EMR of gastric adenomatous lesion. After EMR, perforation in stomach was detected by peumoperitoneum on plain upright chest X-ray. We treated with conservative management such as withholding of oral intake, adminstration of broad-spectrum antibiotics, parenteral nutrition and close monitoring. There were no worsening clinical sings of peritoneal inflammation except mild leukocytosis during the in-hospital course. On the third hospital day after EMR, the patient resumed oral intake. The seventh hospital day, he was discharged. Our experience showed that selected small microperforation in stomach after EMR could be treated by not endoscopic procedures or surgical interventions but conservative management.

전두측두엽 치매의 행동 및 심리적 증상

강수진,이병화,김은주,박기정,나덕렬 대한치매학회 2004 Dementia and Neurocognitive Disorders Vol.3 No.2

Background: Frontotemporal dementia (FTD) usually presents with behavioral and psycho-logical symptoms of dementia (BPSD) rather than cognitive deficits. Thus, understanding of BPSD associated with FTD is important not only from the management perspective but also from the diagnostic viewpoint. However, few studies have investigated BPSD in patients with FTD in Korea. The aim of the current study is to describe in detail BPSD associated with FTD. Methods: Firstly, a BPSD list was designed based on the symptoms reported in previous studies of FTD, items from the Neuropsychiatric Inventory (NPI), and the review of medical records of FTD patients from our clinic including FTD patients not selected in this study. Then an interviewer asked caregivers whether the BPSDs listed had been present from onset of disease to the time of interview. Subjects who participated in the interview consisted of 23 patients (9 men and 14 women) with a mean age of 65.6 (range, 41-75) years who met the FTD criteria proposed by the Lund and Manchester group. The BPSD list was completed by face-to-face interviews or by telephone interviews with the caregivers. Results: BPSDs as classified by subscales of the NPI in the order of frequency were aberrant motor behavior (95.6%), apathy (95.6%), disinhibition (91.3%), appetite/eating change (91.3%), agitation/aggression (87%), euphoria (65.2%), anxiety (60.9%), irritability/lability (52.1%), delusions (43.5%), night-time behavior (39.1%), depression/dysphoria (34.8%), hallucinations (0%). Other symptoms not listed in the NPI were stereo-typical behaviors as stereotypy of ordering, hoarding, reading signboards, wandering a fixed route, washing, and counting, utilization behaviors, and sundowning. Conclusion: Our detailed description of BPSD associated with Korean FTD patients would help understand symptoms of FTD and develop a Korean version of BPSD questionnaire for FTD in the future studies.

B747-400 항공기의 Missed Approach 비행자료 분석

신대원,박종혁,은희봉 한국항공운항학회 2003 한국항공운항학회지 Vol.11 No.2

This study is performed to secure the safety of civil aviation by establishing systematic analysis ability of Flight Data Recorder. Through this study, readouting UFDR(Universa1 Flight Data Recorder) to personal computer, flight data numerical analysis and regulations of Missed Approach. In the analysis, the flight data of B747-400 model aircraft with Missed Approach in San Francisco(KSFO) was selected.

楊楊格 單語와 有意單音節에 對한 統計學的 考察

蘇鎭明,朴聖俊,殷在嬉 대한이비인후과학회 1970 대한이비인후과학회지 두경부외과학 Vol.13 No.2