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급속 응고된 Fe-Mn-Si계 합금 리본의 γ→ε 마르텐사이트변태 특성에 미치는 냉각속도 및 어닐링 온도의 영향
은상원,조무현,장우양 조선대학교 에너지.자원신기술연구소 2004 에너지·자원신기술연구소 논문지 Vol.26 No.2
Effects of colling rate and heat treatment on the microstructure and grain size have been investigated in Fe-Mn-Si based SMA Ribbons, and the correlationship between those intrinsic factors and martensitic transformation charactehstics has been discussed. When Fe-Mn-Si based SMA ribbons were fabricated by RSP, grain size of the ribbon was refined up to 4 ㎛ and the uniformity of grains were improved by increasing wheel speed i.e cooling rate. The volume fraction of thermal ε martensite was increased by increasing colling rate. Refined grains of the melt-spin ribbon were partially grown by annealing above 300℃×3min. By increasing annealing temperature, the volume fraction of thermal e martensite was increased and that of α' martensite were also highly increased in the resultant ribbons. Ms temperature of annealed ribbon was higher than that of as-spun ribbon and transformation temperature hysteresis(A_(f-)M_(s)) was decreased by increasing annealing temperature.
은상원,이재현 조선대학교 생산기술연구소 2005 生産技術硏究 Vol.27 No.2
By applying Eshelby's theory on the 'transformation' and 'inhomogeneity' problems of an ellipsoidal inclusion, a microscopic stress-strain is formulated for a composite material consisting of a matrix and a large number of aligned ellipsoidal inclusions. Some of the composites of practical interest, such as unidirectionally fiber-reinforced, particle dispersion strengthened and layered composites can be treated by changing the axial ratios of the ellipsoidal inclusion. The microscopic stress-strain relation obtained is applicable to elastic and elasto-plastic deformation of the composite in uniform loading.
스퍼터링법으로 TiN 및 ZrN 피막 코팅된 STD 61의 표면특성
은상원(Sang-Won Eun),최한철(Han-Cheol Choe) 한국표면공학회 2010 한국표면공학회지 Vol.43 No.6
STD 61 steel has been widely used for tools, metallic mold and die for press working because of its favorable mechanical properties such as high toughness, and creep strength as well as excellent oxidation resistance. The STD 61 tool steel coated with TiN and ZrN by sputtering results in improvement of wear and corrosion resistance. In this study, surface characteristics of TiN and ZrN film coated STD 61 by sputtering were studied by using FE-SEM, EDS, XRD, and XRR and nanoindentation tests. From the results of surface characteristics of coated specimen, the ZrN coated surface showed finer granular than that of TiN coated surface. The coated layer structures of ZrN and TiN were grown to (111) and (200) preferred orientation. From the results of XRR test for surface roughness, density and growth rate of coating film, surface roughness and growth rate of ZrN coated film revealed lower values those of TiN coated film, whereas density of ZrN coated film showed higher values than that of TiN coated film. From the nanohardness and elastic modulus test, nanohardness value and elastic modulus of ZrN coated film became higher than those of TiN coated film.
산화스트레스가 유도된 TM3 세포에서 통캇알리 추출물이 테스토스테론 생성에 미치는 영향
정재인,은상원,이재경,서영하,배문형,김룡,김은지 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.7
통캇알리 추출물은 선행 동물시험과 인체적용시험에서 테스토스테론을 증가시킴으로써 남성갱년기 증상 개선 가능성이 제시되었으나 테스토스테론 증가 기전에 대해 밝혀지지 않았다. 본 연구에서는 H2O2로 산화스트레스를 유도한 TM3 세포에 통캇알리 추출물이 테스토스테론 수준과 테스토스테론의 생성과 전환에 관여하는 효소의 발현을 조사함으로써 테스토스테론 증가 기전을 밝히고자 하였다. TM3 세포에 50 μM H2O2로 산화스트레스를 유도한 경우는 테스토스테론 수준이 유의적으로 감소하였고, 콜레스테롤 운반체로 작용하는 StAR 발현과 테스토스테론 생성에 관여하는 3β-HSD와 CYP17A1 발현이 유의적으로 감소하였다. 반면 테스토스테론 전환에 관여하는 5α-reductase와 aromatase의 발현은 50 μM H2O2 처리에 의해 유의적으로 증가하였다. H2O2로 산화스트레스를 유도한 TM3 세포에 통캇알리 추출물을 여러 농도(10, 25, 50 및 100 μg/mL)로 처리한 경우 테스토스테론 수준이 유의적으로 증가하였다. 통캇알리 추출물 처리에 의해 StAR, 3β-HSD 및 CYP17A1 발현이 유의적으로 증가하였으나 5α-reductase와 aromatase 발현이 유의적으로 감소하였다. 이는 통캇알리 추출물이 미토콘드리아 내로의 콜레스테롤 이동과 테스토스테론 생성을 증가하고 테스토스테론 전환을 감소함으로써 산화스트레스에 의한 테스토스테론 감소를 개선함을 나타내며, 테스토스테론 감소에 의한 남성갱년기 증상을 개선할 수 있는 가능성을 제시한다.
Cu-Al-Ni계 초탄성 합금의 변태특성 및 변형거동에 미치는 미세조직의 영향
박정수,김경훈,은상원,조무현,장우양,양권승,강조원 조선대학교 생산기술연구소 2004 生産技術硏究 Vol.26 No.2
The effect of microstructure on transformation characteristics and deformation behavior in Cu-Al-Ni based superelastic alloy has been studied in alloy by using metallography, calorimetry, compression test and XRD. When the alloy betatized at the elevated temperature above 600℃, the r₂ phase completely dissolved into the matrix. The β₁' or r₁' martensites from β₁ patent phase was transformed in as-cast state but the parent phase stabilized by betatizing at 600℃. With increasing betatizing temperatures above 600℃. both₁' and r₁' martensites again transformed from β₁ parent phase and the heat flow of r₁' martensite was higher than that of β₁' martensite for reverse transformation. The compressed specimen, showing pseudoelastic behavior, was completely recovered upon unloading. However, the stress-strain curves for compression test were different by betatizing temperature; the stress-strain curves of the specimen betatized at 600℃ and 700℃ were linear but those of the specimen betatized at 800℃ and 900℃ were non-linear. The r₁' martensite was retained after compression test and the volume fraction of that increased with increasing betatizing temperature and strain.
Cu-Al-Ni계 합금 선재의 의탄성 거동에 미치는 미세조직의 영향
장우양,김경훈,은상원,강조원 조선대학교 생산기술연구소 2005 生産技術硏究 Vol.27 No.spec
In order to realize the psuedoelastic behavior of polycrystalline Cu-Al-Ni based alloy, Cu-Al-Ni based alloy rod with unidirectional solidification structure was fabricated by heated-mold continuous casting process. When the cast rod was betatized at the elevated temperature above 700℃, the Y₂ phase is gradually dissolved into the matrix so that the volume fraction of the Y₂ phase was decreased. The compressed specimen was completely recovered upon unloading, resulting in pseudoelastic behavior. The pseudoelastic strain of 10 % could be obtained in cast rod betatized above 700 ℃. However, the stress-strain curves for compression test were not same with betatizing temperature: the stress-strain curves of the specimen betatized at 600 ℃ and 700 ℃ were linear but those of the specimen betatized at 800 ℃ and 900 ℃ were not linear.
Effects of Bonito Elastin HC on Skin Dryness, Wrinkles, and Pigmentation In Vitro and In Vivo
박수정,김다경,이민희,정재은,은상원,김옥경 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.1
We investigated the effects of bonito fish (Katsuwonus pelamis) elastin HC (KE) on skin dryness, wrinkles, and pigmentation in vitro and in vivo. In vitro, we evaluated the expression of mRNA genes and proteins related to skin dryness, wrinkles, and pigmentation. HaCaT and HS27 cells were exposed to ultraviolet B radiation (UVB) (50 mJ/cm2), and B16F10 cells were stimulated with 3-isobutyl-1-methylxanthine (IBMX, 250 μg/mL) for 72 h to induce melanin synthesis. All cells were treated with KE (50–400 μg/mL) for 24 h. We found that KE increased the expression of long-chain base 1, dihydroceramide desaturase 1, elastin, hyaluronan synthase 2, and ceramide synthase 4 mRNA or protein as well as hyaluronic acid and sphingomyelin levels in UVB-irradiated HaCaT cells. Moreover, KE regulated factors related to collagen production, wrinkles, and melanin production in UVB-irradiated HS27 cells and IBMX-stimulated B16F10 cells. In vivo, we evaluated skin hydration and the expression of mRNA genes and proteins in the skin, and conducted morphological observations in SKH-I hairless mice (5-week-old male). The mice were exposed stepwise to UVB and given KE (10, 20, and 30 mg/kg b.w.) for 8 weeks. We found that skin hydration and protein or mRNA expression related to skin moisturization were increased in the KE group. Moreover, KE intake increased factors related to collagen production, wrinkles, and melanin production in UVB-irradiated SKH-I hairless mice. These results suggest that KE may have efficacy for the development of treatments for improving skin health.
조원희,박정진,이민희,박성후,정재은,김진학,은상원,김진경 한국식품영양과학회 2023 Preventive Nutrition and Food Science Vol.28 No.1
This study aimed to investigate whether low molecular fish collagen peptide (FC) from Oreochromis niloticus had protective effects on skin of photoaging mimic models. We observed that FC supplementation improved antioxidant enzymes activities and regulated the pro-inflammatory cytokines [e.g., tumor necrosis factor-α, interleukin (IL)-1β, and IL-6] by reducing the protein expressions of pro-inflammatory factors IkBa, p65, and cyclooxygenase-2 in ultraviolet-B (UV-B) irradiated in vitro and in vivo. Furthermore, FC increased hyaluronic acid, sphingomyelin, and skin hydration by increasing the mRNA expression of hyaluronic acid synthases 1∼3, serine palmitoyltransferase 1, delta 4-desaturase, sphingolipid 1, and protein expressions of ceramide synthase 4, matrix metalloproteinase (MMP)-1, -2, and -9. In UV-B irradiated in vitro and in vivo, FC up-regulated the protein expression of the c-Jun N-terminal kinase, c-Fos, c-Jun, and MMP pathways and up-regulated that of the transforming growth factor-b receptor I, collagen type I, procollagen type I, and small mothers against decapentaplegic homolog pathways. Our results suggest that FC can be effective against UV-B induced skin photoaging by improving skin dryness and wrinkle formation through antioxidant and anti-inflammatory properties.