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Lim, Da-Rae,Kim, Hye-Ryung,Park, Min-Ji,Chae, Ha-Gyeong,Ku, Bok-Kyung,Nah, Jin-Ju,Ryoo, So-Yoon,Wee, Sung-Hwan,Park, Yu-Ri,Jeon, Hyo-Sung,Kim, Ji-Jeong,Jeon, Bo-Young,Lee, Hyeong-Woo,Yeo, Sang-Geon,Pa ELSEVIER SCIENCE DIVISION 2018 JOURNAL OF VIROLOGICAL METHODS Vol.260 No.-
<P><B>Abstract</B></P> <P>A sensitive and specific swarm primer-based reverse transcription loop-mediated isothermal amplification (sRT-LAMP) assay for the detection of serotype O foot-and-mouth disease virus (FMDV) was developed and evaluated. The assay specifically amplified the <I>VP3</I> gene of serotype O FMDV, but did not amplify the <I>VP3</I> gene of other serotype FMDVs or any other viruses. The limit of detection of the assay was 10<SUP>2</SUP> TCID<SUB>50</SUB>/mL or 10<SUP>3</SUP> RNA copies/μL, which is 100 times lower than that of the RT-LAMP assay without swarm primers. The new assay is 10 times more sensitive than reverse transcription-polymerase chain reaction (RT-PCR) and is comparable to the sensitivity of real time RT-PCR (qRT-PCR). Evaluation of the assay using different serotypes of FMDV strains showed 100% agreement with the RT-PCR results. The previously reported serotype O FMDV-specific RT-LAMP assay did not detect 20 out of 22 strains of serotype O FMDVs, probably due to multiple mismatches between the primer and template sequences, showing that it is not suitable for detecting the serotype O FMDVs circulating in Pool 1 region countries, including Korea. In contrast, the developed sRT-LAMP assay with improved primers can rapidly and accurately diagnose serotype O FMDVs circulating in Pool 1 region countries and will be a useful alternative to RT-PCR and qRT-PCR.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A swarm primer-applied RT-LAMP assay was developed for the detection of serotype O FMDV. </LI> <LI> The assay was 10 times more sensitive than RT-PCR and comparable to the sensitivity of qRT-PCR. </LI> <LI> The swarm primer RT-LAMP assay showed 100% agreement with conventional RT-PCR. </LI> </UL> </P>
생활계 기원 유가자원의 분리배출 및 수거 품목 확대에 대한 검토
전다래 ( Da Rae Jeon ),홍수열 ( Soo Yeol Hong ),배재근 ( Chae Gun Phae ) 한국환경정책학회 2011 環境政策 Vol.19 No.4
In 1992, the separate collection of recycling resources started, and the law on resource saving and recycling promotion to encourage it was issued. Also, Extended Producer Responsibility(EPR) has been practiced from 2003, and separate collection items increased and recycling systemwas revved up focused on cooperatives. In recent times, issues such as high oil price and resource circulation become serious and recycling of scrapped materials, etc also becomes important to implement a low-carbon resource recycling community, and thus the necessity of expanding separation and disposal items is strongly emphasized. So it is necessary to re-sort household waste and examine its possibility of separation and disposal by re-evaluating environmental and economic feasibility. Therefore, this study lists allwaste bymaterial or by item, applies quantified evaluation criteria, and selects target items to be further separated and disposed in order to disinter waste resources which are necessary to be added to target items of separation and disposal, examine if they can be targets of separation, disposal, and recycling in the domestic waste. This study recommends some ways to build recycling system that is available to reduce economic and environmental loads in the current disposal system with respect to the selected target items. In addition, thiswork seeks to find a subject of business and operation scheme ranging fromtarget-itemcollection and transport stage to recycling stage.
과채류 부산물의 발생 및 처리 현황 조사를 통한 자원 재활용량 산출
전다래 ( Da-rae Jeon ),배재근 ( Chae-gun Phae ),조준연 ( Jun-yeon Cho ),박성민 ( Sung-min Park ),김도완 ( Do-wan Kim ),이종수 ( Jong-soo Lee ),정혜윤 ( Hye-yoon Jeong ) 한국폐기물자원순환학회(구 한국폐기물학회) 2017 한국폐기물자원순환학회 추계학술발표논문집 Vol.2017 No.-
2005년부터 음식물류 폐기물 분리배출제도 도입을 비롯하여 음식물류 폐기물의 자원화 방안에 대해 여러 가지 방법이 강구되고 있다. 이러한 자원화 노력으로 되어왔지만, 조리 전 생 쓰레기 형태의 음식물류 폐기물에 대해 구체적인 재활용 방안이 없는 실정이다. 과채류의 유통경로에 따라 생산현장 및 가공현장에서 발생하는 세척, 다듬기 후 발생하는 부산물은 사료의 원료로 활용가능하나, 그 동안 적절한 유통체계 없이 사료로 활용되거나 퇴비화 또는 소각처리 하고 있다. 본 연구에서는 과채류 부산물의 정의를 세우고, 농림축산식품부의 농수축산물 표준코드 및 기타 관련 논문을 통해 과채류 부산물의 분류 기준을 마련하였다. 전국 공영도매시장의 과채류 부산물 처리 현황을 조사하고, 시장 유형에 따라 대표 지역을 선정하여 과채류 부산물의 유통구조와 과채류 부산물 발생량을 추정하였다. 전국 4개 시장과 N 대형마트의 물류센터를 대상으로 과채류 부산물을 구분하고, 삼성분, 발열량, 원소분석 등을 측정하였고, 시장 내 중도매인 180여명을 대상으로 과채류 종류별 부산물 발생량 설문 조사를 실시하였다.
LC-MS/MS를 이용한 엇갈이배추 중 Fluxapyroxad와 대사물 M700F002, M700F048의 분석법 연구
전다래 ( Da Rae Jeon ),최종우 ( Jong-woo Choi ),김정한 ( Jeong-han Kim ) 한국환경농학회 2019 한국환경농학회 학술대회집 Vol.2019 No.-
Fluxapyroxad는 carboxamide계 살충제로 참외, 가지, 참깨 등의 흰가루병, 잎마름병, 잿빛무늬병 등 광범위한 농작물에 이용되고 있으나 엇갈이배추에는 그 분석법이 미흡한 실정이다. 본 연구에서는 농약품목등록 자료에 활용 하고자 fluxapyroxad와 대사물 M700F002 및 M700F048의 엇갈이배추 중 잔류 분석법을 검토해 보았다. LC-MS/MS (Shimadzu LCMS-8040)에 표준물질을 주입하여 scheduled MRM (multiple reaction monitoring)조건을 설정하였으며 fluxapyroxad는 Kinetex C18 (2.1 × 100 mm, 5μm, Phenomenex)컬럼을, 대사물(M700F002, M700F048)은 Hypercarb (2.1 × 100 mm, 5μm, Thermo)컬럼을 이용하여 기기조건을 제시하였다. 시료전처리를 위하여 시료 10 g에 0.2 % formic acid가 첨가된 acetonitrile 10 mL를 넣고 1분간 격렬히 진탕한 후 4 gMgSO<sub>4</sub>, 1 g NaCl을 첨가하여 다시 1분간 진탕하였다. 3500 rpm에서 5분 동안 원심분리 후 상층액 1 mL를 취해 dispersive-SPE (MgSO<sub>4</sub> 150 mg, C18 25 mg)에 첨가하여 1분간 진탕한 다음 13,000 rpm에서 4분 동안 원심분리 하였다. 상등액 200 μL와 acetonitrile 200 μL를 혼합하여 matrix matched 시료를 제조하여 LC-MS/MS로 분석하였다. 위 분석법에 따른 Fluxapyroxad와 대사물의 분석정량한계(method limit of quantitation; MLOQ)는 각각 25ng/mL (fluxapyroxad), 50 ng/mL (M700F002, M700F048)이었다. Matrix matched 방법을 이용한 검정곡선의 범위는 fluxapyroxad에서 5 ~ 200 ng/mL, 대사물은 10 ~ 200 ng/mL이었으며, 검정곡선의 상관계수(r2)는 모두 0.99 이상을 만족하였다. 분석법의 검증을 위하여 무처리 시료에 fluxapyroxad와 대사물 M700F002와 M700F048를 MLOQ의 10배, 20배로 처리하여 회수율 시험을 진행 한 결과 모두 회수율범위 70 ~ 120 % (RSD < 10 %)를 만족하였다. 따라서 본 연구에서 제시한 분석법을 통하여 엇갈이배추에 대한 fluxapyroxad 및 대사물 M700F002와 M700F048의 잔류 모니터링에 활용이 충분하다고 본다.
Jeon, Yoo Sang,Shin, Hyun Mu,Kim, Yu Jin,Nam, Da Yeon,Park, Bum Chul,Yoo, Eunmin,Kim, Hang-Rae,Kim, Young Keun American Chemical Society 2019 ACS APPLIED MATERIALS & INTERFACES Vol.11 No.27
<P>Barcode nanowires (BNWs) composed of multiple layered segments of different materials are attractive to bioengineering field due to their characteristics that allow the adjustment of physicochemical properties and conjugation with two or more types of biomolecules to facilitate multiple tasks. Here, we report a metallic Fe (iron)-Au (gold) BNW-based platform for capturing CD8 T cells and the interferon-γ (γ) they secrete, both of which play key roles in controlling infectious diseases such as tuberculosis, at the single-cell level. We also describe an efficient approach for conjugating distinct antibodies, which recognize different epitopes to appropriate materials. The platform achieved detection even with 4.45-35.6 μg mL<SUP>-1</SUP> of BNWs. The T cell capture efficiency was close to 100% and the detection limit for interferon-γ was 460 pg mL<SUP>-1</SUP>. This work presents a potential guideline for the design of single-cell immunoassay platforms for eliminating diagnostic errors by unambiguously identifying disease-relevant immune mediators.</P> [FIG OMISSION]</BR>
연구논문 : 지역기반 폐기물 자원순환 네트워크 기본모형 구상 ; 서울시 노원구의 생활계 폐기물을 중심으로
정회성 ( Hoi Seong Jeong ),김태용 ( Tae Yong Kim ),김철민 ( Chul Min Kim ),배재근 ( Chae Gun Phae ),전다래 ( Da Rae Jeon ) 한국환경정책학회 2013 環境政策 Vol.21 No.1
The objective of this study is to develop a ``RegionalWaste CirculationNetworkModel`` targeting the household waste of Nowon-gu, Seoul Metropolitan City as a case region. The current policies based on the ``Top-downWaste CirculationNetworkModel`` do not reflect the regional characteristics, and thus don`t work efficiently for the household waste management of the regional government. Considering this situation, this research is meant to establish a ``Bottom-upWaste Circulation NetworkModel`` for the target region. For this purpose, we first tried to figure out what the region`s household wastes consist of who the stakeholders are andwhatmanagement route thewaste goes through. Depending on these data, we then established the ``Waste Management Code System``. Second, using the ``Waste Management Code System`` we developed a ``Regional Waste Circulation Network Model`` reflecting regional situations. Then, we applied this model to the household waste management system of Nowon-gu. The Nowon-gu system is composed of two types of modules. One is a household waste management system for the administration. The other is a network system to link the waste stakeholders in the region. In addition, this model is to provide relayed stakeholderswith a statistical information on householdwaste in the region. It can also be utilized as one of the alternatives to save energy and resources at the local level in its efforts to overcome environmental problems.