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Detection of Mycoplasma felis from the kenneled cats with pneumonia
Hong, Sunhwa,Lee, Hak-Yong,Kim, Tae-Wan,Kim, Okjin The Korean Society of Veterinary Service 2015 韓國家畜衛生學會誌 Vol.38 No.1
Two cats were obtained from a cat kennel. Over the previous 7 days, the cats had shown cough, anorexia, depression and nasal discharge. In this study, the consensus PCR was able to detect successfully Mycoplasma species in nasal swab samples of the cats. To identify feline mycoplasma species from the lung tissue of the cats with pneumonia, Mycoplasma species-specific PCR reactions were conducted. As the results, we could identify M. felis by the positive amplified DNAs. On the other hand, we could not detect any positive reactions with the PCR reaction for M. arginini, M. canis, M. edwardii, M. cynos, M. gateae, M. maculosum, M. molared, M. opalescens, M. spumans and Mycoplasma HRC-689. In conclusion, we detected M. felis from the kenneled cats with pneumonia. We suggested that this consensus PCR would be useful and effective for monitoring Mycoplasma species in various kinds of animals including cats. The application of preceding consensus PCR before the species-specific PCRs may be the most recommended strategy for the identification of Mycoplasma spp.
Spontaneous Ovarian Mucinous Adenocarcinama in a Mongolian Gerbil (Meriones unguiculatus)
Sunhwa Hong,Hyun-A Lee,Okjin Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.4
An ovarian mucinous adenocarcinoma was found in a 5-month-old female Mongolian gerbil (Meriones unguiculatus). The animal was evaluated because of slowly progressive abdominal enlargement. Postmortem examination revealed clear, pale, straw-colored fluid in the peritoneal cavity. The left ovary was very huge size as 2x5x3 cm and nodular shaped. Histologically, the tumor was composed of various sizes of multilocular cystic glands lined by a single layer of mucin-filled epithelium. Each of these cystic glands was surrounded by a large amount of solid fibrous stroma resembling smooth muscle. Also, undifferentiated tumor cells were found frequently. The ovarian surface epithelium showed partial invasiveness into the ovarian cortex, and a transition was observed between the surface epithelium and the mucinous cyst-forming epithelium. On the basis of histopathological findings, this case was diagnosed as an ovarian mucinous adenocarcinoma. To our knowledge, this report may be the first case of mucinous adenocarcinoma in Mongolian gerbil.