수종의 광독성 약물투여 후 시간에 따른 피부농도 및 최소 광독량에 관한 연구

박윤기,조무연,한승경,임성빈 ( Yoon Kee Park,Moo Yon Cho,Seung Kyung Hann,Sung Bin Im ) 대한피부과학회 1991 大韓皮膚科學會誌 Vol.29 No.5

N/A The Skin Concentration and Minimal Phototoxic Dose Following Administration of Phototoxic Drugs as a Function of Time Yoon-Kee Park, M.D., Moo Yon Cho, M.D., Saung Kyung Hann, M.D., Sungbin im, M.D. Department of Dermatology Yonsei Univeraity College of Medicine, Seoul, Korea Psoralen photochemotherapy(PUVA) is defined as a treatment that depends on the interaction of a photosensitizing substance, psoralen, and long wave jltraviolet(UVA) radiation that results in therapeutically beneficial effects. The rationale of its use is to clear skin disease by repeated controlled phototoxic reactions. The efficacy of PUVA depends on the drug, the UVA dose and wave length, the individual sensitivity to the phototoxic reaction and on the disease to be treated. However, the major factor is the amount of psoralen at the site of photochemical reaction in skin. Daspite the extensive experiences which have now accumulated in the use of PUVA, the relationship between the therapeutic response and phototoxicity as it occurs in normal skin is not established. Comparative data on different psoralen molecules and their skin sensitizing ability are required. From studies on the skin concentration using HPLC and minimal phototoxic dose following oral administration, intraperitoneal administration, a bath of phototoxic drugs as a function of time in guinea pigs, the following results are obtained. 1. The skin concentration of phototoxic drugs after oral administration peaked at 1.5 hours, and the concentration of 8-methoxypsoralen(8-MOP) was 3.7 times greater than that of 5-methoxypsoralen(5-MOP). The skin concetration of 4,5,8-trimethylpsoralen(TMP) was not detected in this study(limit of sensitivity 5ng/g). 2. The skin conentration of phototoxic drugs after intraperitoneal administration peaked at 30 minutes, and the concentration of 8-MOP was slightly greater than that of 5-MOP. The skin concentration of TMP was not detected in this study(limit of sensitivity 5ng/g). 3. The skin concentration of phototoxic drugs after bathing peaked immediately after bathing, and the skin concentrations of phototoxic drugs after bathing decreased in the order of 5-MOP, TMP, and 8-MOP. 4. The minimal phototoxic dose of phototoxic drugs after oral administration was lowest at 1.5 hours, and the minimal phototoxic dose of 8-MOP was 8 times less than 5-MOP, 24 times less than TMP. The phototoxicity of 8-Mop after oral administration was strongest. The time of lowest phototoxic dose and highest skin concentration was the same. 5. The minimal phototoxic dose of phototoxic drugs after intraperitoneal administration was lowest at 30 minutes, and the minimal phototoxic dose of 8-MOP was 1.3 times less than 5-MOP, 5.7 times less than TMP. The phototoxicity of 8-MOP after intraperitoneal administration was strongest. The time of lowest phototoxic dose and highest skin concentration was same. 6. The minimal phototoxic dose of phototoxic drugs after bathing was lowest at 15 minutes. The minimal phototoxic dose of 8-MOP was not significantly different but was significantly greater than that of 5-MOP.

C3H 생쥐에서 자외선 B의 집중조사와 분할조사가 표피내 랑게르한스 세포의 회복에 미치는 영향

구상완,한승경,박윤기,조무연 ( Sang Wahn Koo,Seung Kyung Hann,Yoon Kee Park,Moo Yon Cho ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.4

N/A The Effect on the Recovery of Epidermal Langerhans Cells in C3H Mice after Single and Fractionated Exposure of Ultraviolet B Irradiation Sang-Wahn Koo, M.D., Seung Kyung Hann, M.D., Yoon-Kee Park, M.D., Moo Yon Cho, M.D. Department of Dermatology, Yonsei University College of Medicine Seoul, Korea The study was undertaken to see the recovery in epidermal Langerhans cells in relation to time after UVB irradiation through different amounts and ways of exposure in C3H mice. We irradiated the ears of C3H mice with UVB 200mJ/㎤ and 400mJ/㎤ in a single dose at one time and 5 fractionated doses for 5 days and 10 fractionated doses for 10 days and performed biopsies on the ears of the control and experimental groups after, 2, 7, 14, 21 days of irradiation and stained them by the immunoperoxidase method. The results are summarized as follows. 1. A more significant decrease in the number of Ia-positive epidermal Langerhans cells was observed in single-dose-exposed group than fractionated-dose-exposed groups on the second day of irradiation. 2. On the seventh day of exposure, the number of the Ia-positive epidermal cells in single-dose-exposed group returned to normal, but in fractionated-dose-exposed groups the number of Langerhans cells decreased most remarkably. 3. In fractionated-dose-exposed groups, the number of Langerhans cells returned to normal on the 14th or 21st day of expposure. (Kor J Dermatol 29(4) : 459-465, 1991)

강원도지역에서 발생한 발진열과 쯔쯔가무시병

민창홍,장우현,강재승,조수익,최문기,조민기,윤창순,김윤원 대한감염학회 1988 감염 Vol.20 No.2

In Korea, there has been an increasing concern on rickettsiosis as one of most prevailing acute febrile illness, since murine typhus and scrub typhus had not been reported for 2 decades until 1986 and thereafter a few studies were reported on these diseases. A microimmunofluorescence test was used to study antibodies to R. typhi and R. tsutsugamushi in urban and rural inhabitants students, and patiants suspected of leptospirosis or rickettsiosis from 1986 to 1987 in Kangwon-do Korea. 43% of inhabitatnts and 17% of students reacted to R. typhi. Only one inhabitant among inhabitants and students reacted to R. tsutsugamushi. Among 169 patients, 43(25%) reacted to R. typhi, 38(22%) to R. tsutsugamushi and 25(15%) to Leptospira interrogans. Sexual rate(male; female) in murine typhus was 1.7:1, that in scrub typhus was 1:2.2 and that in leptospirosis was 1.5;1. The age ditribution of patients with murine typhus was generally even over 20 years old. But 71% of patients with scrub typhus were over 40 years old. Scrub typhus was most prevailing in female over 40 years old. Murine typhus was relatively more prevailing in Choonchun city than rural area as compared with scrub typhus and leptospirosis. Most of scrub typhus occurred in rural area. Most of each disease occurred between September and November (murine typhus; 93%, scrub typhus; 95%, leptospirosis; 84%) with a peak in October. Scrub typhus considerably occurred not only October(58%) but also November(28%). All patients with scrub typhus were immediately diagnosed by antibody when they visited hospital. 53% of patients with murine typhus were diagnosed when they visited hospital within 7 days after onset of illness, and others were done by second sera obtained 8 days after. The patients in whom IgM was seronegative but antibodies of other class were seropsitive by 20 days after onset of illness, were 14% in murine typhus and 26% in scrub typhus. At least those patients were considered to be infected second. Murine typhus and scrub typhus were suggested to be endemic diseases in Kangwon-do as antibodies of inhabitants and characteristics of patients' sera were considered. By serologic finding of patients with scrub typhus in Kangwon-do, Gilliam was 59'% of etiologic strain of scrub typhus, Karp was 41q% and Kato was none. Most scrub typhus occurred in person to be related to agriculture, but murine typhus was not related to job and occurred more in small city than rural area. Rash was scarcely detected in murine typhus and scrub typhus, and eschar was in 50% of scrub typhus only. Serum transaminase elevated in many patients with scrub typhus and murine typhus. Leukocytosis and abnormal finding in chest X-ray were showed in some patients with scrub typhus but were scarcely in murine typhus.

貧血症과 赤血球過多症에 있어서 血淸 Erythropoietin 活性度에 關한 硏究

尹起英,李夏白,朴鍾茂 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.2

Erythropoietin (Ep) is a glycoprotein hormone of mostly renal origin that stimulates red cell production in response to tissue hypoxia. Serum Ep activities provide important clinical clues in making the differential diagnosis of some types of anemia and polycythemia. The study was performed in order to evaluate the diagnostic value of serum Ep activities in 29 normal subjects, 43 anemic patients and 15 secondary polycythemia patients and to find out the relationship between hemoglobin concentration and serum Ep activities in these patients using the Enzyme Immunoassay with Second International Preparation for Ep. The results obtained were as follows: 1. Ep activities in sera from 29 normal subjects among children ranged from 11 to 72 mU/ml, with a mean of 37.10±16.54 mU/ml. There was no significant difference between the serum Ep activities in male and female subjects (p>0.05). 2. Ep activities in sera from 22 patients with iron deficiency anemia ranged from 48 to 400 mU/ml, with a mean of 153.5 mU/ml which was about 4 times higher than normal value. Ep activities in sera from 18 anemic patients with acute infection ranged from 35 to 290 mU/ml, with a mean of 134.05 mU/ml which was about 3.5 times higher than normal value. Ep activities in sera from 3 patients with aplastic anemic ranged from 620 to 840 mU/ml, with a mean of 720 mU/ml which was about 20 times higher than normal value. 3. Ep activities in sera from 15 patients with secondary polycythemia ranged from 12 to 140 mU/ml. In 10 of 15 patients, the mean Ep activities was 87.50 ± 25.60 mU/ml, which was about 2 times higher than normal value. In 5 of 15 patients, Ep activities were within normal range as a mean of 35.80 ± 17.23 mU/ml. In summary, a significant negative correlation was found between the hemoglobin concentration and the logarithm of the serum Ep activities in 43 patients with anemia (r=0.73, Y=3.6766-0.1802x) and Ep activities in patients with aplastic anemia was higher than those in patients with iron deficiency anemia at comparable hemoglobin concentration. From the above results, it was concluded that serum Ep activities in anemic patients are not only inversely related to the hemoglobin concentration but also to the activity of the erythroid bone marrow. While no significant correlation was found between hemoglobin concentration, hematocrit percentages and Ep activities in 15 patients with secondary polycythemia (r=0.18, 0.39 respectively).

관상동맥 스텐트 내 재협착의 발생에 미치는 임상적 관동맥 조영술적 요인들

윤혁준,김기식,박형섭,이영수,현대우,한성욱,허승호,김윤년,김권배,박남희,원경숙 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.12

실측자료를 이용한 교각 세굴방정식의 비교

윤영호,김종섭,연기석 大田産業大學校 1997 한밭대학교 논문집 Vol.14 No.1

Over the past 30years, numerous studies have been conducted and equations developed to Predict bridge-pier scour. Most of these equations were developed using laboratory data and sometimes tested using limited field data. For a number of the equations, the conditions under which the laboratory experiments were conducted are unknown. In addition, the ranges of the various parameters over which the equations are valid are also typically unknown. Even if the range of data used in the laboratory study is known, it is quite uncertain as to flow those ranges, using small-scale models, correlate to prototype conditions. The purpose of this study is to compare seven bridge-pier-scour equations using field data. Data for live-bed scour will be examined; most floods the stream bed is live, there are cases. The limitaions of each of the equations will be examined for the purpose of providing guidance to hydraulic engineers concerned with scour at bridge piers.

개 대뇌겉질에서 Platelet-Derived Growth Factor α-Receptor의 출생 후 발달에 관한 면역조직화학적 연구

윤영,안병수,김인정,양경철,박선홍,김기훈,박도영,김장만,문정석,장인엽,조하영 조선대학교 부설 의학연구소 2002 The Medical Journal of Chosun University Vol.27 No.1

Background and Objectives : The localization of platelet-derived growth factor-α receptor (PDGF-α R) was commonly restricted to oligodendrocyte progenitors during late embryonic and postnatal development. However, several studies recently demonstrated that mature neurons could also synthesize PDGF-α, Materials and Methods : In the present study, to analyze the distributional pattern of PDGF-αR during postnatal development of the canine cerebral cortex, we used immunohistochemistry on sections of canine brain tissue. Results : We found that neurons of various regions of cerebral cortex exhibited the immunoreactivity to PDGF-αR as early as postnatal day 0, and slightly decreased after postnatal day 14. Whereas neuronal PDGF-αR were maintained at all ages, the oligodendroglia-like expression of PDGF-αR could not be confirmed. Conclusion : The localization of PDGF-αR in immature and mature neurons supports the several roles of PDGF during development, protection and survival of neurons.

혈장 중 theophyline 분석법 확립 및 약동학적 연구

김형기,권준택,염윤기 순천향대학교 의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

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三國遺事의 說話的 性格과 文藝樣式에 관한 연구

윤기홍 연세대학교 대학원원우회 1987 원우론집 Vol.14 No.2

골다공증 척추 골절에서 전방 감압술 및 기기 이용 전방 유합술

하기용,류승준,윤형문 대한척추외과학회 2003 대한척추외과학회지 Vol.10 No.3