CLOCK and BMAL1 stabilize and activate RHOA to promote F-actin formation in cancer cells

Teng-jiao Ma,Zhi-wei Zhang,Yilu Lu,Ying-ying Zhang,Da-chang Tao,Yun-qiang Liu,Yong-xin Ma 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Circadian genes control most of the physiological functions in cancer cells, including cell proliferation, migration, and invasion. The CLOCK and BMAL1 complex plays a central role in circadian rhythms. Previous studies have shown that circadian genes may act as oncogenes or tumor-suppressor genes. In addition, F-actin, regulated by RHOA, has been shown to participate in tumor progression. However, the roles of the CLOCK and BMAL1 genes in the regulation of tumor progression via the RHOA-ROCK-CFL pathway remain largely unclear. Here we first indicate that the rearrangement of F-actin is regulated by CLOCK and BMAL1. We found that CLOCK and BMAL1 can upregulate RHOA expression by inhibiting CUL3-mediated ubiquitination and activate RHOA by reducing the interaction between RHOA and RhoGDI. Consequently, CLOCK and BMAL1 control the expression of the components of the RHOA-ROCK-CFL pathway, which alters the dynamics of F-actin/G-actin turnover and promotes cancer cell proliferation, migration,

Synthesis of SrTiO3 Fibers and Their Effects on the Thermoelectric Properties of La0.1Dy0.1Sr0.75TiO3 Ceramics

Jiao Han,YiMing Zeng,Ying Song,HongQuan Liu 대한금속·재료학회 2019 ELECTRONIC MATERIALS LETTERS Vol.15 No.3

SrTiO 3 fi bers were fabricated by an in situ hydrothermal method using hydrated TiO 2 fi bers as both template and reactant. La 0.1 Dy 0.1 Sr 0.75 TiO 3 powders containing x wt.% SrTiO 3 fi bers ( x = 0, 1, 3, 5) were prepared by the sol–gel method andthen sintered at 1450 °C under a reducing atmosphere (N 2 /H 2 = 95/5). XRD analysis showed that the samples were mainlycomposed of SrTiO 3 phase and a few Dy 2 Ti 2 O 7 phase. TiO 2 phase was detected in the samples with x = 3 and x = 5, and itspeak intensity clearly reinforced with increasing x . With the addition of SrTiO 3 fi bers, the electrical conductivity increasedsignifi cantly and the Seebeck coeffi cient kept almost unchanged, resulting in a high power factor of 1015 μW m −1 K −2 at200 °C with a loading of 3 wt.% SrTiO 3 fi bers. Meanwhile, combined with low thermal conductivity, the sample with 3 wt.%SrTiO 3 fi bers showed the peak ZT value of 0.19 at 500 °C, which was 127% higher than that of La 0.1 Dy 0.1 Sr 0.75 TiO 3 .

Changpingibacter yushuensis gen. nov., sp. nov., isolated from fluvial sediment in Qinghai Tibet Plateau of China

Jiao Yifan,Zhang Sihui,Yang Jing,Lai Xin-He,Dong Kui,Cheng Yanpeng,Xu Mingchao,Zhu Wentao,Lu Shan,Jin Dong,Pu Ji,Huang Ying,Liu Liyun,Wang Suping,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2

Two facultatively anaerobic, short rod-shaped, non-motile, Gram-stain-positive, unknown bacterial strains (JY-X040T and JY-X174) were isolated from fluvial sediments of Tongtian River in Yushu Tibetan Autonomous Prefecture, Qinghai province, China. Cells formed translucent, gray, round and convex colonies, with a diameter of less than 0.5 mm after 5 days of incubation at 30°C on brain heart infusion-5% sheep blood agar. The 16S rRNA gene sequence similarity between strain JY-X040T and Fudania jinshanensis 313T is 93.87%. In the four phylogenetic trees constructed based on the 16S rRNA gene and 423 core genes, the two isolates form an independent branch, phylogenetically closest to F. jinshanensis 313T, but could not be classified as a member of the genus Fudania or any other genus of the family Arcanobacteriaceae. The DNA G + C content of strain JY-X040T was 57.8%. Calculation results of average nucleotide identity, digital DNADNA hybridization value and amino acid identity between strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%, and 64.1%. The major cellular fatty acids were C16:0 (23%) and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α (L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and four unidentified components. The whole-cell sugars contained rhamnose and ribose. MK-10(H4) was the sole respiratory quinone. The minimum inhibitory concentration of streptomycin was 32 μg/ml. All physiological, biochemical, chemotaxonomic and genomic characteristics support that strains JY-X040T and JY-X174 represent members of a novel species in a new genus, Changpingibacter yushuensis gen. nov., sp. nov. The type strain is JY-X040T (GDMCC 1.1996T = KCTC 49514T).

Static behavior of high strength friction-grip bolt shear connectors in composite beams

Ying Xing,Yanbin Liu,Caijun Shi,Zhipeng Wang,Qi Guo,Jin-feng Jiao 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.42 No.3

Superior to traditional welded studs, high strength friction-grip bolted shear connectors facilitate the assembling and demounting of the composite members, which maximizes the potential for efficiency in the construction and retrofitting of new and old structures respectively. Hence, it is necessary to investigate the structural properties of high strength friction-grip bolts used in steel concrete composite beams. By means of push-out tests, an experimental study was conducted on post-installed high strength friction-grip bolts, considering the effects of different bolt size, concrete strength, bolt tensile strength and bolt pretension. The test results showed that bolt shear fracture was the dominant failure mode of all specimens. Based on the loadslip curves, uplifting curves and bolt tensile force curves between the precast concrete slab and steel beam obtained by push-out tests, the anti-slip performance of steel-concrete interface and shear behavior of bolt shank were studied, including the quantitative analysis of anti-slip load, and anti-slip stiffness, frictional coefficient, shear stiffness of bolt shank and ultimate shear capacity. Meanwhile, the interfacial anti-slip stiffness and shear stiffness of bolt shank were defined reasonably. In addition, a total of 56 push-out finite element models verified by the experimental results were also developed, and used to conduct parametric analyses for investigating the shear behavior of high-strength bolted shear connectors in steel-concrete composite beams. Finally, on ground of the test results and finite element simulation analysis, a new design formula for predicting shear capacity was proposed by nonlinear fitting, considering the bolt diameter, concrete strength and bolt tensile strength. Comparison of the calculated value from proposed formula and test results given in the relevant references indicated that the proposed formulas can give a reasonable prediction.

Ontogenetic Expression of Lpin2 and Lpin3 Genes and Their Associations with Traits in Two Breeds of Chinese Fat-tailed Sheep

Jiao, Xiao-Li,Jing, Jiong-Jie,Qiao, Li-Ying,Liu, Jian-Hua,Li, Liu-An,Zhang, Jing,Jia, Xia-Li,Liu, Wen-Zhong Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.3

Lipins play dual function in lipid metabolism by serving as phosphatidate phosphatase and transcriptional co-regulators of gene expression. Mammalian lipin proteins consist of lipin1, lipin2, and lipin3 and are encoded by their respective genes Lpin1, Lpin2, and Lpin3. To date, most studies are concerned with Lpin1, only a few have addressed Lpin2 and Lpin3. Ontogenetic expression of Lpin2 and Lpin3 and their associations with traits would help to explore their molecular and physiological functions in sheep. In this study, 48 animals with an equal number of males and females each for both breeds of fat-tailed sheep such as Guangling Large Tailed (GLT) and Small Tailed Han (STH) were chosen to evaluate the ontogenetic expression of Lpin2 and Lpin3 from eight different tissues and months of age by quantitative real-time polymerase chain reaction (PCR). Associations between gene expression and slaughter and tail traits were also analyzed. The results showed that Lpin2 mRNA was highly expressed in perirenal and tail fats, and was also substantially expressed in liver, kidney, reproductive organs (testis and ovary), with the lowest levels in small intestine and femoral biceps. Lpin3 mRNA was prominently expressed in liver and small intestine, and was also expressed at high levels in kidney, perirenal and tail fats as well as reproductive organs (testis and ovary), with the lowest level in femoral biceps. Global expression of Lpin2 and Lpin3 in GLT both were significantly higher than those in STH. Spatiotemporal expression showed that the highest levels of Lpin2 expression occurred at 10 months of age in two breeds of sheep, with the lowest expression at 2 months of age in STH and at 8 months of age in GLT. The greatest levels of Lpin3 expression occurred at 4 months of age in STH and at 10 months of age in GLT, with the lowest expression at 12 months of age in STH and at 8 months of age in GLT. Breed and age significantly influenced the tissue expression patterns of Lpin2 and Lpin3, respectively, and sex significantly influenced the spatiotemporal expression patterns of Lpin3. Meanwhile, Lpin2 and Lpin3 mRNA expression both showed significant correlations with slaughter and tail traits, and the associations appear to be related with the ontogenetic expression as well as the potential functions of lipin2 and lipin3 in sheep.

The Determinants and their Time-Varying Spillovers on Liquefied Natural Gas Import Prices in China Based on TVP-FAVAR Model

( Ying Huang ),( Yusheng Jiao ) 한국정보처리학회 2024 Journal of information processing systems Vol.20 No.1

China is playing more predominant role in the liquefied natural gas (LNG) market worldwide and LNG import price is subject to various factors both at home and abroad. Nevertheless, previous studies rarely heed a multiple of factors. A time-varying parameter factor augmented vector auto-regression (TVP-FAVAR) model is adopted to discover the determinants of China’s LNG import price and their dynamic impacts from January 2012 to December 2021. According to the findings, market fundamentals have a greater impact on the import price of natural gas in China than overall economic demand, financial considerations, and world oil prices. The primary determinants include domestic gas consumption, consumer confidence and other demand-side information. Then, there are diverse and time-varying spillover effects of the four common determinants on the volatility of China's LNG import price at different intervals and time nodes. The price volatility is more sensitive and long-lasting to domestic natural gas pricing reform than other negative shocks such as the Sino-US trade war and the COVID-19 pandemic. The results in this study further proves the importance of domestic natural gas market liberalization. China ought to do more to support the further marketization of natural gas prices while working harder to guarantee natural gas supplies.

Static behavior of bolt connected steel-concrete composite beam without post-cast zone

Ying Xing,Yun Zhao,Qi Guo,Jin-feng Jiao,Qing-wei Chen,Ben-zhao Fu 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.38 No.4

Although traditional steel-concrete composite beams have excellent structural characteristics, it cannot meet the requirement of quick assembly and repair in the engineering. This paper presents a study on static behavior of bolt connected steel-concrete composite beam without post-cast zone. A three-dimensional finite element model was developed with its accuracy and reliability validated by available experimental results. The analysis results show that in the normal service stage, the bolt is basically in the state of unidirectional stress with the loss of pretightening can be ignored. Parametric studies are presented to quantify the effects of the post-cast zone, size and position of splicing gap on the behavior of the beam. Based on the studies, suggested size of gap and installation order were proposed. It is also confirmed that optimized concrete slab in mid-span can reduce the requirement of construction accuracy.

Nitrogen and Fluorine Dual-Doped Carbon Nanosheets for High-Performance Supercapacitors

Chen Jiao,Zhong Jie Zhang,Xiang Ying Chen 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.4

Doping carbon materials with heteroatoms such as N, F is an effective approach to elevating the capacitive performance of supercapacitors. In this paper, nitrogen and fluorine dual-doped two-dimensional (2D) porous carbon nanosheets (PCNSs) have been fabricated by a straightforward template carbonization method, using trisodium citrate as carbon source and self-template, and ammonium fluoride as N/F dopants. The N/F-doped carbon samples are well characterized by a series of techniques and measured in a three-electrode system and two-electrode system, respectively. As a result, N/F-doped carbon has delivered large capacitance of 110 F g -1 at 1 Ag -1 and high-energy density of 3.82 W h kg -1 at the power density of 0.5 kW kg -1. It is also revealed that semi-ionic C–F bonds in PCNSs have enhanced electrical conductivity, hence, facilitating electron transport in the electrode. For comparison, ammonium chloride is used as sole dopant for producing N-doped carbon materials, whose capacitive performances are much lower than the N/F-codoped one, indicating the synergistic effect of N/F for capacitive improvement.

Xanthoceraside Induces Apoptosis in Melanoma Cells Through the Activation of Caspases and the Suppression of the IGF-1R/Raf/MEK/ERK Signaling Pathway

Qing Jiao,Libo Zou,Peng Liu,Qian Xu,Yifei Zhang,Ying Yu,Lu Zou,Tianyan Chi,Xuefei Ji 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.10

Xanthoceraside, a saponin extracted from the husks of Xanthoceras sorbifolia Bunge, suppresses inflammation and oxidative stress. However, the antitumor properties of xanthoceraside as well as its mechanism of action remain unclear. Therefore, we proposed to investigate its potential anticancer property. In this study, the viability of cells was measured by the MTT assay. Cell cycle and mitochondrial membrane potential were measured by flow cytometry, and the expressions of procaspase-9, procaspase-3, Cyto.c, Apaf-1, Bcl-2, Bcl-xL, Bad, p53, and IGF-1R/Raf/MEK/ERK were tested by Western blotting. Xanthoceraside significantly inhibited the proliferation of human melanoma A375.S2 cells in a concentration- and time-dependent manner but did not impair the viability of normal cells (peripheral blood mononuclear cells). Further analysis revealed that xanthoceraside induced apoptosis by activating caspase-3 and caspase-9 in a time-dependent manner through the mitochondrial pathway but did not activate caspase-8 in the cells. In addition, xanthoceraside inhibited the expression of the insulin-like growth factor-1 receptor (IGF-1R), which is an important prosurvival, antiapoptotic signaling growth factor receptor that is frequently overexpressed in cancer cells and used as a therapeutic target for multiple cancers. Interestingly, xanthoceraside also decreased the expression of Raf, p-MEK, and p-ERK, the downstream effectors of IGF-1R. Taken together, these findings indicate that xanthoceraside induces apoptosis through a mitochondria-mediated apoptotic pathway, which is induced by the downregulation of IGF-1R/Raf/MEK/ERK cascades in A375.S2 cells.

Anisotropic In-plane Resistivity and Magnetoresistance of the Detwinned BaFe2As2

Lin Jiao,Zong Fa Weng,Xue Yan Tang,Lu Kai Guo,Tian Shang,Lin Yang,Hui Qiu Yuan,Yu Ying Wu,Zheng Cai Xia 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.3

We have measured the electrical resistivity and the magnetoresistance of detwinned BaFe2As2single crystals in fields up to 16 Tesla. The temperature dependence of the resistivity shows acounterintuitive anisotropic behavior in the ab-plane, likely arising from the effect of the nematicsusceptibility. Little magnetoresistance is observed at temperatures above the structural/magnetictransition, below which a huge in-plane magnetoresistance with an anisotropy is detected.