Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway

Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.

[논문]ZCS를 이용한 간단한 long pulse CO2 레이저 전원

김태균,이임근,김희제 釜山大學校生産技術硏究所 2004 生産技術硏究所論文集 Vol.63 No.-

30Torr 이하 저압 공진기에서 ms order의 long pulse duration을 가진 간단한 펄스 CO2 레이저를 구현하였다. 레이저 시스템의 새로운 전원은 직접 상용전원을 스위칭한다. 이 새로운 전원의 방전 회로에는 정류기, 축전용 커패시터, 또는 전류 제한용 저항이 필요하지 않다. 레이저 출력을 제어하기 위해 펄스 반복률을 최대 60Hz까지 조절했고,SCR의 스위칭각은 30°에서 150°까지 변화시켰다. ZCS circuit과 PIC one-chip microprocessor는 SCR 게이트 신호를 제어하기 위해서 사용되었다. 18 Torr 압력,60Hz 의 펄스 반복률,SCR 스위칭각 90°에서 최대 레이저 출력 35 W를 얻었다. 또한 다른 CO2 레이저와 비교해서 long 펄스를 얻을 수 있었고 이는 대략 3ms(FWHM) 였다.

BAM(Brewster Angle Microscope)으로 관측한 Langmuir막의 상전이에 따른 분자 배향에 관한 연구

조환제,강도원,박태곤 창원대학교 산업기술연구소 2001 産技硏論文集 Vol.15 No.-

In this study, we used Brewster-Angle Microscope(BAM) to study on the molecular orientation of monolayer on the water surface. The domains by behaviors of molecules on three different subphase have been observed. Reproducible π-A isotherm have been obtained only on information about phase transition by molecular area. BAM facilitates the observation of morphology by optical anisotropy and thickness in monolayer and multilayers as BAM is shown to be sensitive to anisotropy of film. Also, the coalescence of domains leads to large regions of uniform tilt-azimuthal direction. Every transition was found by BAM technique, either as a dramatic change in degree of contrast or as a sudden alteration of molecular action and π-A isotherm.

발모광(拔毛狂,Trichotillomania)1례

김제헌,유태열 大韓神經精神醫學會 1988 신경정신의학 Vol.27 No.5

Trichotillomania is defined as a compulsion to pull-out strands of hair from the head, sometimes from the pubic area. This symptom arises as a part of symptom complexes in a variety of psychiatric disorders such as obsessive compulsive disorder, depression, borderline personality, mental retardation and schizophrenic disorder. The authors experienced a case of trichotillomania, an isolated symptom as a part of obsessive compulsive disorder in a 8 year old boy. Several literatures were reviewed and psychiatric aspects of trichotillomania were discussed. For the treatment of this patient, haloperidol and supportive psychotherapy had been tried and been proven effective.

Sucrose density gradient에 의해 분리된 C형 간염 바이러스의 특징 분석(Ⅰ)

황태욱,유병제 대구대학교 기초과학연구소 2000 基礎科學硏究 Vol.16 No.2

To study the buoyant density of HCV, the human patient sera was tested with both anti-HCV ELISA and RT-PCR for the detection of HCV. The patient sera, positive at two tests, were used for further experiments. After ultracentrifugation of 20-60% sucrose linear density gradient, the centrifugal medium containing HCV was fractionated by 280 ul from the bottom. The buoyant densities of all fractions were determined, and the HCV in all fractions were checked by the RT-PCR. There was the room to separate the fractions to two major parts containing HCV. One was the high density part (1.161 g/ml - 1.192 g/ml), and the other was the low density part (1.085 g/ml - 1.117 g/ml).

폐주불사를 혼입한 콘크리트의 내구성에 관한 연구

김태경,박정호,이정호,이주형,박제선,김연경,윤경구 강원대학교 석재복합신소재제품연구센터 1998 석재연 논문집 Vol.3 No.-

콘크리트 구조물은 자연적인 혹은 인위적인 온도의 상승, 강하로 인하여 동결-융해 작용을 받게 되어 구조물의 성능저하를 야기시킨다. 현대에 들어서면서 콘크리트의 내구특성을 파악하기 위한 연구적 요구가 증가되고 있다. 따라서 폐주물사 콘크리트를 실제 구조물에 적용하기 위해서는 내구특성에 대한 연구가 반드시 이루어져야 한다. 폐주물사를 혼입한 콘크리트의 내구특성을 파악하기 위한 동결-융해 실험은 폐주물사의 잔골재 치환율, 물-시멘트비, AE제 사용 여부를 주요 변수로 하여 실시하였다. 원형공시체를 제작하여, 동결-융해 시험기에 넣어 -18∼4℃로 급속 동결-융해를 진행시키면서 매 23싸이클마다 동탄성계수를 측정하였다. 실험결과 AE제를 첨가했을 때 물-시멘트비가 적을수록, 폐주물사의 치환율이 클수록 전반적으로 강도가 증가하는 것으로 나타났으며, 물-시멘트비가 증가함에 따라, AE제를 사용한 콘크리트가 동결-융해 저항성이 증가하는 것으로 나타났다. 특히 폐주물사 치환율이 50%일 때가 동결-융해 저항성이 가장 좋은 것으로 나타났으며 그 다음으로 25, 0% 순으로 저항성이 좋은 것으로 나타났다. 따라서 폐주물사 콘크리트가 내구성이 우수한 것으로 나타나 폐주물사를 콘크리트에 재활용 할 수 있는 것으로 나타났다. Concrete structures has been deteriorated by the freezing and thawing due to temperature gap. This study was conducted to evaluate durability of concrete which are increasingly demanded recently. Therefore the research of durability must be executed for application of waste foundry sand concrete real structures. Concrete durability properties incorporating waste foundry sand was performed with the variable of W/C ratio, Sand/Waste foundry sand ratio and Air entrainment-Non air entrainment. Cylinder specimens were made and subjected to freezing and thawing cycle at -18℃ and 4℃. Dynamic modulus of elasticity were evaluated as F/T cycle increase. The results show that strength of concrete is increased the W/C ratio decrease, the Sand/Waste foundry sand ratio increase when the concrete contains AE agent and decreasing W/C ratio and AE concrete makes improved resistance of freezing and thawing improved. Especially, resistance of freezing and thawing is improved by Fine aggregate/Waste foundry sand ratio which is 50%, 25%, 0% in a row. Therefore it is turn out the waste foundry sand could be applied to concrete from the experiment.

직업적 디메틸아세트아미드(Dimethylacetamide) 노출에 의해 집단적으로 발생한 독성간염

최태성,우극현,김진석,박완섭,함정오,정상재,유재영 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

목적 : DMAC에 노출된 후 집단적으로 발생한 독성 간염 7례보고. 방법 : 2000년 2월 신설된 스판덱스 섬유제조 공장에 근무하는 생산직 근로자 178명중 2000년 2월 부터 8월까지 7명이 간장질환이 발생하였다. 환례들의 나이는 23∼47세였고, 남자 5명, 여자 2명이었다. 이들에 대해 혈액검사, 간 초음파 검사 등의 임상검사와 작업장 조사를 실시하였다. 결과 : 근무 부서는 중합 1명, 방사 1명, 권취 4명, 그리고 포장 1명이며 평균근무기간은 10주였다. 주 호소 증상은 심한 피로감, 어지럼증 및 황달이었고, 검사실 소견상 독성간염의 소견을 보였다. A, B 및 C형 간염 표지자 검사는 모두 음성이었고, 간초음파 소견상 특이 소견이 없었다. 문진 상 알코올 성 간염을 일으킬 정도의 음주력이나 최근 약물 복용력을 가지고 있는 환례는 없었다. 입원 후 보존적인 치료로 모두 급속히 호전되었다. 초기에 발생했던 환례에서 퇴원후 DMAC에 재 노출되어 독성 간염이 재발되었다. 이후 모든 환례들은 퇴원 후 DMAC 비노출 부서로 전환하였고, 퇴원후 지속적인 추적검사에서 간 효소 수치가 완전히 정상화되었고, 정상화되는 기간은 1∼2개월 정도 소요되었다. 결론 : 환례들의 임상증상, 검사결파, 노출력 및 과거력상 DMAC에 의한 독성간염으로 추정된다. Dimethylacetamide is widely used in the production of plasics, resins, synthetic fibers, and gums and in purification and crystallization processes. Inhalation of the vapor or skin absorption of the liquid of dimethylacetamide (DMAC) can cause liver damage. Toxic hepatitis possibly attributable to DMAC exposure occurred in seven works among 178 employees who had worked on a new spandex-fiber production line. A large amount of DMAC is used as a spinning solvent for synthetic fibers in the factory. The patients were aged 23-47 years old and composed of five males and two females. They were involved in the process of polymerization(1 patient), spinning(1), take-up(4) and packaging(1). The mean duration of exposure was 10 weeks. They experienced fatigue, dizziness and jaundice. The patients showed elavated total bilirubln, alanlne aminotransferase (ALT), and aspartate aminotransferase (AST) levels. The serologic test for viral hepatitis A, B and C were negative, as were the abdominal ultrasonographic scans. Based on the presumptive diagnosis of DMAC-induced toxic hepatitis, they were removed from the workplaces. One or two months after removal from the work, the transaminase levels returned to normal. The patients had no history of significant alcohol use, blood transfusion, recent medication, and drug abuse. As a result, authors could not find any attributable cause of toxic hepatitis but the toxicity by DMAC exposure.

C형 간염 바이러스의 세포배양(Ⅰ)

황태욱,유병제 大邱大學校附設 基礎科學硏究所 2000 基礎科學硏究 Vol.17 No.2

In this research, It was investigated to increase the infectious efficacy of HCV to culture cells, and the efficacy of HCV cell culture system. To increase the infectious efficacy to human cells, The human hepatoma cell lines (Huh_(7),) was used as the culture cells to test the HCV infection. The whole patient serum, the high density portion, and the low density portion were added in the culture media of human hepatoma (Huh_(7)) cells as the infectious sources. It was showed that HCV partcles of the high density portion were not infectious to Huh_(7) cells, but HCV particles of the low density portion were infectious to Huh_(7) cells. Therefore, it was concluded that the antibody bound HCV was not infectious to Huh_(7) cells, but the antibody free HCV was infectious to Huh_(7) cells.

계층화분석법을 이용한 노후 공동주택의 재건축과 리모델링 의사결정방법

이태영,장한두,제해성 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(계획계)

In this study, it is tried to establish a integrated evaluation method composed of functional criteria, economical criteria and characteristic criteria of apartment housing, the latter is additional consideration. Functional and economical criteria is similarly effected in decision making to respectively apartment housings with different characteristics by functional evaluation and profit analysis. There is necessity for recognizing that decision making between reconstruction and remodeling of apartment housing must be performed as not single development project but consideration of residential environment to improve the quality of residents' environment in long-range insight. Therefore, meaning of this study is to find out the possibility of application about characteristics of apartment housing as criteria than to make a practical evaluation method.

C형 간염 바이러스의 세포배양(Ⅱ)

황태욱,유병제 대구대학교 기초과학연구소 2000 基礎科學硏究 Vol.17 No.2

In this research, It was investigated to increase the infectious efficacy of HCV to culture cells, and the efficacy of HCV cell culture system. To increase the infectious efficacy to human cells, The human hepatoma cell lines (Huh_(7)) was used as the culture cells to test the HCV infection. The whole patient serum, the high density portion, and the low density portion were added in the culture media of Huh_(7) cells as the infectious sources. In the high density portion, the HCV genomes were not detected in the media and cells of all days except in the 2 day culture medium, but in the low density portion, the HCV genomes were detected in the media and cells of all days. It was showed that HCV partcles of the high density portion were not infectious to Huh_(7) cells, but HCV particles of the low density portion were infectious to Huh_(7) cells. Therefore, it was concluded that the antibody bound HCV was not infectious to Huh_(7) cells, but the antibody free HCV was infectious to Huh_(7) cells.