Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

1,1-Diphenyl-2-methyl-1,2-propanediol의 산촉매 자리옮김

백영숙 慶熙大學校 1994 論文集 Vol.23 No.-

1,1-Diphenyl-2-methyl-1,2-propanediol 1 was synthesized to study the effect of solvents on the acid-catalyzed rearrangement reaction. Compound 1 was treated with concentrated sulfuric acid, acetic anhydride with trace amounts of sulfuric acid, trifluoroacetic acid, and various concentrations of trifluoroacetic acid/acetic acid. The products of compound 1 in these solvents system were examined by NMR spectra. The product ratios of 3,3-diphenylbutanone 2 and α,α-dimethylpropiophenone 3 were dramatically changed in different acidic media or even in different ratios of trifluoroacetic acid/acetic acid solution; the ratio of methyl migration to phenyl migration was 97:3 in trifluoroacetic acid and 59:41 in 30/70 trifluoroacetic acid/acetic acid. It was concluded that the effect of solvent is very important factor for the study of pinacol rearrangement.

유아기 그림 유우머 설명정도 및 감지정도와 창의성과의 관계

백영숙,손혜숙 경인여자대학 1997 경인논집 Vol.- No.6

The aim of this study is an attempt to understand children more effectively through the relationship between comprehension of pictorial humor and creativity in early childhood. Children's explanation of pictorial humor was analysed to ascertain : (1) the effect of age on the children's explanation of pictorial humor stimuli, (2) the effect of age on the children's appreciation of pictorial humor stimuli, (3) the rating of creativity on children's explanation of pictorial humor and (4) the rating of creativity on children's appreciation of pictorial humor. For this purpose, the explanation of pictorial humor and the appreciation of pictorial humor and integrated creativity were tested. Three major themes were used by Kang(1982). They consisted of themes such as taboo, aggression and sophistication. These themes were used in the current study to construct three pairs of drawings as humor stimuli. The children's explanations of pictorial humor were classified into four categories originally developed by Brown (1993). To measure creativity, the Integrated Creativity Test for four to six-year-old Korean Children developed by Chun (1995) was used. The subjects of this experiment were 90 four to six-year-old children who attended Kindergartens in Seoul and Pundang. The children were individually given the Humor Stimuli and Creativity Test. The analysis of data employed a oneway ANOVA and the Duncan test significance through SPSS/PC+. The results and conclusions of this study are as follows. 1) There were partly significant age differences in the rating of children's explanation of pictorial humor. That is, the six-year-old group showed higher explanation levels than the four-year-old and the five-year-old groups. 2)There were no significant age differences in the rating of children's appreciation of pictorial humor. That is, the higher old age group didn't show higher appreciation levels than lower old age groups. 3) There were significant differences in the explanation levels of pictorial humor in the rating of creativity. That is, the higher explanation levels of pictorial humor group ?? showed higher creativity scores than the lower explanation levels of pictorial humor group children. 4) There were no significant differences in the appreciation levels of pictorial humor in the rating of creativity. That is, the higher appreciation levels of pictorial humor group children didn't show higher creativity scores than the lower appreciation levels of pictorial humor group children. The above-mentioned findings were interpreted and discussed in terms of theoretical background and previous research findings. Some suggestions were presented for further study.

^13C-enriched Tunichrome Mm-2의 합성

백영숙 慶熙大學校 1992 論文集 Vol.21 No.-

^13C-enriched blood pigment of tunicate, Mm-2, was synthesized in which hydroxyl groups and amino group were protected with t-butyldimethylisilyl groups and t-butoxycarbonyl group, respectively. Vanillin-carbonyl-^13C was demethylated and the hydroxyl groups in the resulting 3,4-dihydroxybenzaldehyde-carbonyl-^13C were protected with t-butyldimethylsilyl chloride. Horner-Emmons Wittig reaction, in which bis-O-TBDMS-protocatechualdehyde-carbonyl-^13C was reacted with phosphonate in the presence of base, was employed to produce ^13C-enriched E-isomer as a major product. ^13C-enriched Z-isomer, the desired product, was obtained by photoisomerization of ^13C-enriched E-isomer using Rayonet RPR-3000 A˚fluorescent lamp.

청소년 정신분열증 환자의 어머니의 단기집단 정신치료의 연구

곽영숙,백기청,고기형 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.1

To evaluate schizophrenic mothers' conflict, depression, and attitude toward their schizophrenic children and how they perceive psychiatric illness, we studied group process of nine mothers in twelve sessions. The results were as follows : 1) There was significant difference in only atmosphere of family in PBR. 3) There was partial changes in cause, treatment and perspective of disease. 4) Drop-out rate was 10.1%. 5) Late attendance rate was 2.8 persons per session and abscence rate was 0.8 person per session. 6) Group silence was 0.9 frequency per session and there was great difference to authority and style of therapist. 7) Laughter rate and crying rate were 0.6 and 0.4 frequency, respectively. 8) Familial attendance was 0.3 person per session except mothers. 9) There was overt depression during group psychotherapy. 10) Uncooperation of therapist made group process to involve only therapist. 11) Goup cohesion and universality were major group dynamics, which dependency and helplessness appeared.

일산화탄소 폭로후 고압산소 재폭로시 흰쥐 신장 및 심장의 SOD 활성 변동에 관한 연구

전영희,백두진,황세진,박준숙,정호삼 한양대학교 의과대학 1997 한양의대 학술지 Vol.17 No.1

Carbon monoxide is generated from the incomplete combustion of carbon-containing materials. Carbon monoxide cause muscle necrosis leading to myoglobinuria and acute renal failure. The hyperbaric oxygen exposure is increased production of active free radicals. Free radicals which promoted peroxidation of unsaturated fatty acids may destroy essential cellular components and itself cytotoxic materials. Superoxide dismutase, one of the protective mechanisms against tissue damage, catalyzed the dismutation of superoxide anion to less toxic product. The author performs the present study to investigate on Mn-SOD and Cu, Zn-SOD immunoreactivities in kidney and heart carbon monoxide (CO) and hyperbaric oxygen (HBO) exposure. The animals were divided CO exposure group, air release after CO exposure group, HBO exposure group and, CO and HBO exposure group. The specimen were sectioned in 16㎛ with cryostate. The Mn-SOD and CuZn-SOD immunohistochemistry was performed in renal corpuscle, proximal and distal convoluted tubules of kidney and, in sarcoplasm and sarcolemma of heart muscle. The results obtained were as follows: 1. The immunoreactivity of Mn-SOD were increased in renal corpuscles and distal convoluted tubules, were similar to control group in proximal convoluted tubules. 2. The immunoreactivity of Cu, Zn-SOD were increased than that of control group in renal corpuscles and distal convoluted tubules, were same that of the control group in proximal convouted tubules. 3. The immunoreactivity of Mn-SOD were increased at all experimental group of sarcoplasms of heart muscle. 4. The immunoreactivity of Cu,Zn-SOD were increased than that of control group in sarcolemma of heart muscle. These results suggest that the immunoreactivity of SOD after CO or HBO exposure is greatly increased in kidney and heart.

측모두부방사선 사진을 이용한 아동 위턱뼈굴 성장의 누년적 고찰

박영석,백기석,장미숙,이승표 대한해부학회 2004 Anatomy & Cell Biology Vol.37 No.2

치자 청색소의 빛 안정성 연구

이창민,백영숙 경희대학교 자연과학종합연구원 2000 자연과학논문집 Vol.6 No.-

Iridoid glycoside인 gemposide를 치자(Gardenia jasminoides) 열매로부터 분리, 정제한 후 β-glucosidase로 가수분해하여 genipin을 얻었다. 생성된 genipin을 phosphate 완충용액(pH 7.0)에 녹인 다음 glycine을 가하고 70℃에서 5시간동안 반응시켜 청색소를 얻었다. 치자 청색소의 빛에 대한 안정성을 알아보기 위하여, 청색소를 다양한 농도의 알코올 함유 수용액에 녹인 다음 5,000, 10,000, 그리고 20,000 lux의 강한 빛을 10 시간 동안 조사한 결과, 청색소의 잔존율이 우수함을 확인할 수 있었다. Geniposide obtained from fruits of Gardenia jasminoides was hydrolyzed with β-glucosidase to yield genipin. Genipin was transformed to blue pigments by the reaction with glycine. The stability of the blue pigments against light (5,000, 10,000, and 20,000 lux) was studied to examine gardenia blue dye as a possible alcoholic beverage colorant.

혈액투석 환자의 가족지지, 무력감과 자가간호와의 관계 연구

이강오,이미숙,백영주 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1

This study was designed to investigate the correlations between family support, Powerlessness. and self-care of hemodialysis patients. One hundred thirty seven hemodialysis patients with chronic renal failure from 2 university hospitals, 2 general hospitals, and 2 medical cilinics in Kwang-ju. were accidental sampled. The data were collected from September 16 to 30, 1995. by the questionnaire method. The instrment used for measuring family support was Kang's Family Support Scale, powerlessness was developed by the researcher from the literature review, and self-care was developed by Kim and Youm and was modified by the resecher. The data were analyzed by t-test, ANOVA, and Peatson's correlation coefficient. The results of the study were as follows: 1. Average family support score of subjects was 33.71. 2. Average powerlessness score of subjects was 70.03. 3. Average seof-care score of subjects was 63.28. 4. Relatinoships between general characteristics and variables were as follows 1) There was sighificant difference between family income and family support(F=6.90.p=.000). 2) Three were significant difference betweem present family income and family support(F=6.90, p=.000), 3) There was significant difference between medical insurance and self-care (t= 2.52, p=.013). 5. Correltions betweem family support, powerlessness, and self-care were as follows 1) The first hypothesis, "the higher the level of family support in patients on hemodialysis, the lower the level of powerlessness will be," was supported(r=-.233. p=.022). 2) the second hypothesis, "the higher the level of family support in patients on hemodialysis, the higher the level of self-cate will be." was supported(r=.235, p=.019). 3) The third hypothesis, "the lower the level of powerlessness in patients on hemodialysis, the higher the level of self-cate will be," was not supported(r=-.033. p=.740).

일반인과 정신과 환자 보호자들의 정신질환에 대한 인식과 태도에 관한 연구

박정우,곽영숙,백기청 大韓神經精神醫學會 1995 신경정신의학 Vol.34 No.4

This study was conducted to examine the knowledge and attitude toward mental illness in the lay people and the psychiatric patients' caretakers from December 1993 to October 1994. 242 lay people who were the parents of the students in an elementary school and 164 psychiatric patients' caretakers at Seoul National Mental Hospital were asked to answer a questionnaire about mental illness. In addition, parent Bonding Instrument(PBI) was used to determine the parental bonding style in 94 psychiatric patients' caretakers. And the results were as follows : 1) the primitive, supernatural concepts toward mental illness were significantly high in caretakers and in low educational group. 2) The psychological, environmental concepts toward mental illness were significantly high in female and in low educational group. 3) The negative attitudes toward the mentally ill were significantly high in female caretakers and in older age group and how educational group. 4) The caretakers' negative attitude toward the mentally ill were significantly associated with increased number of admissions. 5) The Mothers had more psychological, environmental concepts and more negative attitudes toward mental illness than the siblings in caretakers. 6) The negative attitudes toward the mentally ill were significantly associated with high level of parental overprotection and low level of parental care and the primitive, supernatural concepts toward mental illness were significantly associated with high level of paternal overprotection and low level of paternal care. We suggest that educational programs on the mental illness, especially for the primary caretakers, are necessary for early detection and treatment of illnesses, prevention of relapses- and rehabilitation.