Generation of Transgenic Pigs Carrying a Construct for Endothelial Specific Expression of CD73

Keon Bong Oh,Bella Kim,Nayoung Ko,Dae-Jin Kwon,Dong-Hoon Kim,Gi-Sun Im,Jin-Ki Park,Seongsoo Hwang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Despite of the absence of hyperacute rejection and acute humoral xenograft rejection, the organ graft of the a1,3-galactosyltransferase (GalT) gene knockouted (KO) and complement regulatory protein (CRP) expressing pig into a nonhuman primate is rejected by development of a thrombotic microangiopathy and/or a consumptive coagulopathy. Thus further introduction of genes to overcome the coagulation incompatibilities between pig and primate under GalT KO/CRP genetic background has been strongly suggested. CD73 (ecto-5'-nucelotidase) is an enzyme attached via a glycosyl phosphoinositol anchor to the extracellular membrane of endothelial cells, which catalyses the hydrolysis of adenosine triphosphate to adenosine. Loss of activity of CD73 results in activation and aggregation of platelets by a reduced capacity to convert nucleotides to adenosine. In previous study, we reported generation of GalT KO fibroblasts concurrently expressing membrane cofactor protein and produced cloned pigs by nuclear transfer of the fibroblast cells (1). In this study, we constructed a vector for expression of human CD73 under control of promoter of pig Icam2 gene expressed specifically at endothelial cells. This vector was introduced into porcine fibroblasts using the nucleofection technology, by which we had forty three fibroblasts clones carrying pIcam2- CD73 vector. Somatic cell nuclear transfer resulted in generation of two transgenic piglets survived.

<見者>의 詩的 旅程 : 鄭鎭圭의 詩世界

高亨鎭 상명대학교 어문학연구소 1995 語文學硏究 Vol.3 No.-

Jeong Jin-Kuy is a leading poet who has published 9 collection of poems and a faithful poet to the classical features of poetry. In his early works he tried to search invisible world rather than visible real world. But he could not the identity of onthology about such a invisible world. The more he passioned for a search for the invisible world, the more he sunk into a abyss of despair and doubt. [Identity], [The most beautiful dust of classic] are clearly showed that point. Therefore the poet turned to a daily world entering 1970's. But it dosen't mean a whole change to the daily world giving up the mentalism of invisible world. In his poem of forming a daily world, the mentalism of search for eternity and immortality was contained. The poet find out a paradoxical truth in daily world. The truth is that to make empty is to fill up and to reach to the world of eternity. [A writting with a pencil], [About a bone] are the good works expressed such a his poetic world. His mentalism has been deeply related to Taoism. Such a poetic attitude come near falling into a philsophical and aphoristical saying. But he shows the real and concrete world of his experience expressing that such a truth lies in just our 'body'. He is a uncompleted poet preparing for more writting works, we don't know how his poem is going for. But on the our study by this time, we can expect that his poem is still going to show the intensive mentalism in spite of making a new poetic world. His mentalistic poem until now has a important meaning of our modern poetic history. During his working period from 60's to early 90's, A sociaty-concerning poetry and A break-form poetry-are widely spread in our modern poetry. On this poetic mood he is evaluated to pursuit of original value of poetry on the ground of mentalism and to maintain a poetic dignity.

인진쑥의 scopoletin함량 분석 및 뇌혈류량에 미치는 영향

고진식 한국전통의학연구소 2006 한국전통의학지 Vol.15 No.1

In this study, Artemisia capillaris, which has been used as oriental medicine area and folks remedy, was investigated for effective substance (scopoletin) analysis. As an attempt to develop new functional beverage by using medicinal herb, Artemisia capillaris. Therefore we investigate the effect of scopoletin in Artemisia capillaris on vessel and regional cerebral blood flow (rCBF) of rats. Artemisia capillaris extract was significantly decreased vessel constraction and significantly increased rCBF. Artemisia capillaris has often been cited in medical literature for its medicinal effects. Although the Korean Food and Drug Administration indicated Artemisia capillaris as the In-Jin-Sook which was allowed as a food stuff. From the view point of the subjects who eat In-Jin-Sook products, most of them ingested it for a certain pharmacological efficacy rather than as a beverage or a food. According to this facts, we need develop functional foods using In-Jin-Sook. Also scopoletin, get known to smooth muscle relaxation content was 326.13 ug/g. These results demonstrate that Artemisia capillaris has pharmaceutical effects and balanced nutritional contents. In according with Artemisia capillaris has a sufficiency of the terms use of food stuff in functional foods.

Distinct function of miR-17-92 cluster in the dorsal and ventral adult hippocampal neurogenesis

Jin, Junghee,Ko, Heejae,Sun, Tao,Kim, Seung-Nam Elsevier 2018 Biochemical and biophysical research communication Vol.503 No.3

<P><B>Abstract</B></P> <P>It has been known that the dorsal and ventral areas of the dentate gyrus in the hippocampus have distinct roles in memory and mood behaviors. We previously reported that microRNA <I>miR-17-92</I> regulates adult hippocampal neurogenesis and mood disorders. Here, we suggest that the <I>miR-17-92</I> cluster is highly expressed in the ventral than the dorsal dentate gyrus in the adult mouse hippocampus. Deletion of <I>miR-17-92</I> in the adult hippocampus only affects development of neural progenitors in the ventral dentate gyrus, and <I>miR-17-92</I> knockout mice have no defects in memory functions. Our results suggest that regional expression of <I>miR-17-92</I> in the dentate gyrus is associated with their distinct functions in hippocampal neurogenesis and related behaviors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> miR-17-92 is highly related with hippocampal neurogenesis and mood behavior. </LI> <LI> The expression of miR-17-92 cluster is regionally different in hippocampus. </LI> <LI> miR-17-92 KO less affect development of neural progenitors in ventral hippocampus. </LI> <LI> Interestingly, memory function was not altered by miR-17-92 KO. </LI> <LI> These findings suggest the distinct function of regional expression of miR-17-92. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Identification of AFLP Marker Linked to a SCN Resistant Gene in Soybean

Ko,Mi-Suk,Kim,Myung-Sik,Han,Soung-Jin,Chung,Jong-Il,Kang,Jin-Ho 한국자원식물학회 2002 Plant Resources Vol.5 No.3

The soybean cyst nematode (Heterodera glycines Inchinoe; SCN) is a devastating pest of soybean and is responsible for significant losses in yield. The use of resistant cultivars is the effective method to reduce or eliminate SCN damage. The objective of this research is to identify AFLP markers linked to the SCN resistant genes. Bulked genomic DNA was made from resistant and susceptible genotypes to SCN and a total of 19 primer combinations were used. About 31 fragments were detected per primer combination. The banding patterns were readily distinguished in resistant and susceptible bulked genotypes. Polymorphic fragments were detected between resistant and susceptible bulked genotypes in the primer combination of CGT/GGC, CAG/GTG and CTC/GAG. In primer combinations of CGT/GGC and CAG/GTG, bulked resistant genotype produced a polymorphic bands. However, in primer of CTC/GAG, bulked susceptible genotype produced a polymorphic fragments. Three AFLP markers identified as a polymorphic fragments between bulked genomic DNA were mapped in 85 F2 population. Among them, only two markers, CGT/GGC and CTC/GAG, was linked and was mapped. Broad application of AFLP marker would be possible for improving resistant cultivars to SCN.

역번역을 활용한 한국 시조 이해 교육 연구 - 영어권 한국어 고급 학습자를 대상으로 -

김영 ( Jin Y Ing ),고정희 ( Ko Jeong-hee ) 연세대학교 언어연구교육원 한국어학당 2020 외국어로서의 한국어교육 Vol.59 No.-

This study aimed to present the availability and implications of back-translation learning methods in Korean sijo education as a foreign language. Results were based on the understanding of Korean sijo by advanced English-speaking Korean learners using back translation. In this study, the significance of back translation as a method of learning sijo is summarized as follows: First, learner-centered activities that encourage learners to participate exist; second, reading native texts can help obtain “advanced knowledge;” third, results are an exploratory process involving learners’ independent thinking; and fourth, awareness of diversity in interpretation encourages freedom to express views on texts. This study selected Hwang Jin-i’s sijo “A Long Night of Winter Solstice” as an experimental text and categorized the understanding of English-speaking advanced learners of the Korean language using back translation into “accepting the meaning of text through reading translations,” “interpretation of the meaning of text through back translations,” and “deeper understanding of the text by comparing back translations with source texts.” Advanced English-speaking Korean learners showed attitudes related to actively organizing the meaning of sijo while performing back translation; they continued to interpret the text by mobilizing their own experience and knowledge through the back translation process. The different order of words in the mother tongue and Korean also led to difficulty in switching to the target language. The following implications were derived based on the understanding of advanced English-speaking Korean learners using back translation. First, the teacher should effectively adjust the primary response the learner sees after reading the translation text. Second, proper educational measures from teachers are needed when learners spend a considerable amount of time on a single poem. Third, the cultural context of learners should be extended after comparing the back translated and source texts. (Seoul National University)

Localization of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal Antibody

Kang-seuk Choi,Jin-ju Nah,Young-joon Ko,Shien-young Kang,Yi-seol Joo 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal AntibodyKang-seuk Choi*, Jin-ju Nah, Young-joon Ko, Shien-young Kang1 and Yi-seok JooNational Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea1Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, 48 Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, KoreaReceived April 2, 2003 / Accept July 10, 2003J. Vet. Sci. (2003), 4(2), 167-173JOURNAL OFVeterinaryScience*Corresponding author: Kang-seuk Choi National Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea Tel: +82-31-467-1860, Fax: +82-31-449-5882 E-mail: choiks@nvrqs.go.kr

관상동맥협착 정도 예측을 위한 경동맥 초음파 검사의 유용성

고광일,박병현,오석규,김남호,조정구,정진원 대한노인병학회 2001 대한노인병학회지 Vol.5 No.4

음향이미지의 개선을 위한 디지틀이미지처리

고대식,최진탁,전계석 慶熙大學校 1988 論文集 Vol.17 No.-

In this paper, the use of versatile digital image storege and processing system for scanning acoustic microscope(SAM system) has been described. In addition to the data acquisition and storage functions, different types of image processing techniques of relevance to scanning acoustic microscope have been studied and analyzed. These include gray level-thresholding and edge enhancement, spatial filtering, and pseudo-color processing algorithms. In experiment, it has been shown that these techniques could enhance particular aspects of the acoustic image of filter out unwanted noise.

憂鬱症의 社會精神醫學的 硏究 : 特히 憂鬱症 發病前 狀況을 中心으로 Especially the Situations as the Precipitating Factor

高珍富 大韓神經精神醫學會 1978 신경정신의학 Vol.17 No.3

1. In this clinical study, it was intended to reveal the socio-environmental events occurring prior to the onset of severs depressions in 12"endogenous" depressives and 26 "nonendogenous" depressies who were hospitalized in the Department of Neuropsychiatry, Chosun University Hospital from April 1974 to August 1976. Among the 38 depressed patients, 11 cases were males and 27 were females. 2. Twenty stressful socio-environmental events were empirically selected according to the frequency of occurrence in the recorded material. Of these 20,14 events that occurred more frequently were then subjected to a more intensive analysis. The more frequent socio-environmental events were defined as follows: 1. Financial affairs 2. Change in family relationship 3. Failure of child ren to meet parent's expectation 4. Beieg forced to encounter negative reality 5. Failure in job performance and increased resgonsijbility 6. There to sexual identity 7. Damage to social status 8. Change in marital relationship 9. Death of important person 10. Moving residence 11. Unemployment and transfer of job 12. Physical illeness 13. Iatrogenic factor 14. Parturition 3. More than 90% of them reported one or more socioenvironmental events; most of them reported multiple stressful events prior to the onset. The male patients tended to report predominantly social factors in contrast to the female patients who reported familial and social events. Frequently reported social events were financial affairs, failure of children to meet parent's expectations, failure in job performance, increased job resporsibility encountering negative social realities and damage to the social status. Relatively infrequent were moving residence, house problems, unemployment and transfer of job. Frequent familial affairs were conflict among extended family members and threat to sexual identity. Disease, death or parting of the family members were infrequently associated with the onset. 4. Patients with repeated history of depression presented similar stressful events prior to each onset. The author noted in each patient a consistent interaction between external events and internal psychological reactions to these events which seemed to be determined by a core conflict and underlying psychopathology.