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      • Genetic Diversity of Korean Rice Breeding Parents as Measured by DNA Fingerprinting with Simple Sequence Repeat (SSR) Markers

        Song,Moon-Tae,Lee,Jeom-Ho,Lee,Sang-Bok,Cho,Youn-Sang,Ku,Ja-hwan,Seo,Kyoung-In,Choi,Seong-ho,Hwang,Heung-Goo 한국자원식물학회 2003 Plant Resources Vol.6 No.1

        Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. Present study was conducted to evaluate the genetic diversity within a diverse collection of rice accessions used for Korean breeding programs. Two hundred eighty-seven rice cultivars, composed of temperate japonica, tropical japonica, indica, and Tongil-type of Korean crossing parents were evaluated by means of 15 simple sequence repeat (SSR) markers. A total of 99 alleles were detected, and the number of alleles per marker ranged from 4 to 11, with an average of 6.6 per locus. Polymorphism information content (PIC) for each of the SSR markers ranged from 0.2924 to 0.8102 with an average of 0.5785. These results, with the result that use of only 15 SSR markers made all rice cultivars examined could be uniquely distinguished, imply the efficiency of SSR markers for analysis of genetic diversity in rice. Cluster analysis was performed on similar coefficient matrics calculated from SSR markers to generate a dendogram in which two major groups corresponding to japonica (Group I) and indica and Tongil type rice (group II) with additional subclasses within both major groups. The narrowness of the Korean breeding germplasm was revealed by the fact that most of the Korean-bred and Japan-bred temperate japonica cultivars were concentrated into only 2 of the sub-group I-1 (143 cultivars) and I-2 (58 cultivars) among six sub-groups in major group of japonica. This is because of the japonica accessions used in this study was a very closely related ones because of frequent sharing of the crossing parents with similar genetic background with synergy effect of the inherited genetic difference between indica and japonica. A rice breeding strategy with the use of molecular markers was discussed for overcoming of genetic vulnerability owing to this genetic narrowness.

      • KCI등재

        알칼리염과 니켈염의 혼합물을 촉매로 한 역청탄 촤의 수증기 가스화 반응특성

        송병호,장용원,변윤섭 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.3

        호주산 역청탄 촤의 수증기 가스화반응에서 알칼리염과 니켈금속염의 혼합물(K_2So_4=Ni(NO_3)_2)이 나타내는 촉매활성을 상압하의 열천칭반응기를 사용하여 온도 650-850℃ 범위에서 조사하였다. 알칼리염 촉매에 니켈금속염을 부가하면 가스화반응속도의 상승효과를 얻을 수 있는데, 반응온도가 낮을수록 그 효과가 크게 나타나 650 ℃에서 약 2배의 반응속도를 얻었다. 본 혼합물은 가스화반응에서 뛰어난 활성을 보이는 K_2CO_3의 활성에는 미치지는 못하지만 경제적인 촉매물질로서의 가능성을 보였다. 가스화반응에서 뛰어난 활성을 보이는 K_2CO_3의 활성에는 미치지는 못하지만 경제적인 촉매물질로서의 가능성을 보였다. 가스화반응의 kineic인자를 도출하기 위하여 여러 가지 기-고체 반응모델을 적용한 결과 화학반응 율속인 수축핵모델과 수정된 체적모델이 가스화반응의 거동을 잘묘사하였다. 역청탄의 촤-수증기 가스화반응의 겉보기 활성화에너지는 99kJ/mol로 얻어졌으며 혼합물촉매를 사용한 촉매가스화반응에서는 84kJ/mol이 얻어졌다. 촤-제조시의 열처리 온도가 가스화반응속도 및 촉매의 활성에 미치는 영향도 고려하였다. The catalytic actibityof a salt mixture of potassium sulfate and nikel nitrate on the steam gasification of a bituminous char at 650-850 ℃ has been measured in an atmosheric themobalance reactor. The mixture of K_2SO_4 and Ni(NO_3)_2 exhibits a synergic effect on the activity and the effect is more pronounced at low temperatures. Double the reaction rate could carbonate which is known to be one of the best catalysts in coal gasification, the mixture does nor reach that of potassium carbonate which is known to be one of the best catalysts in coal grasification, the mixture does not reach that of potassium carbonate which is known to be one of the best catalysts in coal grasification, the mixture seems to be an economically kinetic parameters, the shrinking core model in chemical reaction controlled regime and the modified volumetric model well describe the gasification behavior. The apparent activation energy of non-catalytic gasification was found to be the catalytic activity was also cinsidered.

      • KCI등재

        Glucose Oxidase가 척수지각신경절세포에 미치는 영향과 천마의 효과에 관한 연구

        송호준,이용석,손영우,이강창,정종길,신민교,홍기연 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

        Objectives : To clarify the cytotoxic effect of glucose oxidase(GO) and protective effect of gastrodiae Rhizoma (GR) on spinal sensory ganglion(DRG) neurons, neurotoxicity mediated by GO was measured by MTT assay and neurofilament enzymeimmuno assay(EIA). Methods : DRG neurons were cultured in the media containing various concentrations of GO for 3 hours. In addition, neuroprotective effects of GR, on GO-induced neurotoxicity in DRG neurons were examined after DRG neurons were preincubated with various concentrations of GR for 2 hours before 15mU/ml GO for 3 hours. Results : GO decreased remarkably cell viability in dependently in these cultures, and also RG increased cell viability and amount of neurofilament in DRG neurons damaged by GO. Conclusion : It is suggested that GO has toxic effect in cultured mouse DRG neurons, and also RG was effective in the protection of GO-induced neurotoxicity in these cultures.

      • 왜소음경증과 남성 성기능에 관한 연구

        송윤섭,김민의,이남규,박영호,전윤수,김응구 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

        Micropenis is defined as a normally formed penis with a size greater than 2 standard deviations below the mean. Twenty three patients with micropenis were managed at Soonchunhyang University Hospital from January 1988 to December 1995 We interviewed and examined them with micropenis to investigate the cause of micropenis, concomitant diseases, the efficacy of treatment and their sexual activity in postpubertal men. The patients were divided into 2 groups: 16 prepubertal and 7 postpubertal men. 9 patients had cryptorchidism, 3 of them were bilateral and 5 patients had hypospadias. Etiology was determined by hormonal assay including HCG stimulation test ; 8 as hypogonadotrophic hypogonadism, 4 as primary testicular dysfunction and 5 as idiopathic. However etiology was undetermined in 6 patients. All patients had endocrine therapy using HCG or depot testosterone. All except 4 cases responded satisfactorily. All postpubertal patients(group 2) were below the 10th percentile. Puberty was delayed in 3 patients in the older group. All patients were heterosexual and they have had erections and orgasms. Five patients have had ejaculations, 3 of them were sexually active and they have experienced vaginal penetration, Nevertheless, their sexual partners have had complaints and the patients themselves have felt ashamed and dissatisfied. Fortunately, they were mentally healthy. None of these patients were married or had a child.

      • Effect of Temperature Associated with Early Growth Stimulus on Shortening of Heading Dates in Rice

        Song,Moon-Tae,Lee,Jeom-Ho,Cho,Youn-Sang,Hwang,Hung-Goo 한국자원식물학회 2002 Plant Resources Vol.5 No.2

        The heading date is known to be controlled by two kinds of genetic constituent, photosensitivity and basic vegetable phase. For the latter, the effect of temperature in early growth period is critical to determine the shortness of vegetative growth periods in plant s life. A phytotron experiment on 55 rice cultivars, consisting of two ecotypes of rices, indica and japonica, was conducted at high and low temperature treatments at early growth stage to investigate the possible role of plant growth stimulus by high temperature to associate with shortening of heading date. The high temperature during the early growth stage stimulated the rice growth as measured by plant height with much difference of the growth response between indica and japonica. The conclusive finding that these growth stimulus in early growth stage was highly correlated with the acceleration of heading is, more or less, correlated with the heading of the late growth stage although we could not conclude the genes for early plant growth stimulus by high temperature is the same genes as the genes for accelerating of heading in the late growth stage of plants.

      • 합금철 분진의 재활용을 위한 기초 연구

        신강호,송영준,김윤채 三陟大學校 1998 論文集 Vol.31 No.1

        This study was performed to obtain the basic data for recycling of the dust in ferroalloy manufacture. The chemical composition, composition material, particle size and shapes of the bulk dust, sizing dust and magnetic separating dust were investigated. As the result, the dust from High Carbon Ferromanganese Manufacturing Process is not sufficient as source material of Mn, the dust from Bag Filter of AOD Process is a good source material of Mn, and the dust from Cooler of AOD Process can be a good source material of Mn through Sizing or Magnetic Separation.

      • 망간 용해액의 정제를 위한 기초 연구

        신강호,송영준,김윤채 三陟大學校 1998 論文集 Vol.31 No.1

        This study was performed to obtain basic data for refining manganese nitrate solution which used as starting material for manufacturing Mn₃O₄, raw material of Mn-Zn soft ferrite. 1. Among the various kinds of manganese oxide, MnO is most appropriate for making manganese nitrate solution, for the MnO is easily dissolve in nitric acid and which reaction is exothermic. 2. The most appropriate condition for reduction roasting which reducing Mn₃O₄to MnO is temperature of 700~800℃, roasting time of 30~60min, carbon 5~10 wt.%. 3. The appropriate pH of solution and reaction time for removing iron and silicon from manganese nitrate solution by controlling the pH of solution are pH4~6, 1~2hour, respectively. 4. The appropriate condition for removing calcium and magnesium from manganese nitrate solution by precipitating in the form of oxalate are pH of 1~4, (NH₄)₂C₂O₄adding amount of 0.1M or less and as long reaction time as possible.

      • CALPUFF model과 ISCST3 model을 이용한 악취모델링 기법 연구

        구윤서,김성태,김용규,송선호 안양대학교 산업기술연구소 2002 自然科學硏究 Vol.9 No.-

        악취는 인간의 후각기관에 의해 감지되기 때문에, 대상 지역의 평균농도(Average Concentration) 보다는 악취의 특성상 순간적인 농도인 첨두농도(Peak Concentration)가 중요한 의미를 갖는다. 따라서 이것을 모델링에 적용하기에는 많은 어려움이 있다. 이를 해결하는 것이 이 연구의 주된 과제라 할 수 있다. 현재 국내에서 환경영향평가에 많이 사용되고 있는 ISCST3 Model (Industrial Source Complex Short Term 3 Model)과 CALPUFF Model (California Puff Model)을 사용하여 첨두농도(Peak Concentration)를 고려한 관능악취도(Odor Intensity)를 두 가지 방법으로 산정 하였다. 첫 번째로는 오염원에서 배출되는 물질의 농도를 이용하는 방법과 다른 하나는 종합악취도 개념으로 악취농도(Odor Concentration)를 이용하여 관능악취도를 산정하는 것이다. 위의 두 가지 방법을 ISCST3 Model과 CALPUFF Model에 적용하여 모델링을 수행한 후 나온 기여농도를 첨두농도로 환산한 후 관능악취도를 계산하였다. 이와 같은 이론 배경을 바탕으로 현장 적용성을 파악하기 위해서 수도권매립장의 한 지점에서 가상의 가스배제공이 있다고 가정하고, ISCST3 Model과 CALPUFF Model에 동일한 조건으로 모델링을 수행하여 매립장 주변에 미치는 관능악취도를 각각 산출해 비교 검토하였다. 그 결과 두 모델에서의 차이는 크지 않았으나 기여농도와 첨두농도 사이에는 상당한 차이를 보였다. 결과적으로 악취모델링을 수행함에 있어서 기여농도만을 고려하는 것은 관능악취도를 과소평가 할 우려가 있으므로 배출원 특성을 고려한 첨두농도로 관능악취도를 평가하는 것이 타당하다고 판단된다. In order to manage the odor related public complains effectively, the estimation of peak ground-level concentration which is capable of predicting the intermittent maximum response less than 10 seconds is very important. However, peak rather than 3 minute or 1 hour average concentrations are not easily predicted by current air dispersion models such as SCST3 (Industrial Source Complex Short Term - version 3) and CALPUFF (CALifornia Puff) models. According to the field and wind tunnel measurements, the intensity of the peak concentration depends on the emission source type, downwind distance and atmospheric stability. The odor modeling methods to predict the peak concentration were reviewed and the detailed procedure for the odor modeling was explained in this study. In order to compare the conventional air dispersion models with the oder models, the odor concentrations with various modeling techniques under the identical emission and meterological conditions were calculated. The peak concentration shows highest value than the 3 minutes or 1 hour average concentrations. The predicted odor concentrations by the CALPUFF model are higher than those predicted by the ISCST3 model since the CALPUFF model reflects the time-dependent movement of the plume. It is recommended that using the peak concentration with the CALPUFF model rather than 3 minutes or 1 hour average concentrations is more reasonable approaches to manage the odor problems.

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