한국인 아동에서의 IL-1 유전자 다변성 연구

윤성식,정현주,김옥수,양규호,Yoon, Seong-Sik,Chung, Hyun-Ju,Kim, Ok-Su,Yang, Ku-Ho 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.2

The severe form of chronic periodontitis(CP) has been reported to be strongly associated with the presence of allele 2 of composite IL-1B(+3954) and IL-1A(+4845) genetic polymorphisms(genotype positive). However, other studies have reported conflicting findings. These might have resulted from differences in ethnic background and disease entities. The aim of this study was to determine the distribution of IL-1A(+4845), IL-1B(+3954), IL-1B(-511), and IL-1 RN(VNTR) genetic polymorphisms in children as a future Korean population. The study population consisted of 92 children from the Dept. of Pediatric Dentistry, Chonnam National University Hospital. Genomic DNA was obtained from buccal swab. The IL-1A(+4845), IL-1B(+3954), and IL-1B(-511) genes were genotyped by amplifying the polymorphic region using multiplex polymerase chain reaction(PCR), followed by restriction enzyme digestion and gel electrophoresis. IL-1 RN(VNTR) polymorphism were then evaluated by PCR amplification and fragment size analysis in agarose gel. The allele 2 frequency was 41.3%, 4.3%, 47.8%, and 9.9% for IL-1A(+4845), IL-1B(+3954), IL-1B(-511), and IL-1 RN respectively. The frequency of genotype with allele 2 carriage for IL-1A(+4845), IL-1B(+3954), IL-1B(-511), and IL-1 RN was 77.1%, 7.6%, 63.0%, and 15.2% respectively. The allele 2 frequency in IL-1B(+3954) was significantly higher in female than in male population(p<0.05). The negative association was shown between the presence of allele 2 in IL-1B(-511) and in IL-1B(+3954), and the carriage rate of IL-1B(+3954) allele 2 tended to lower in IL-1B(-511) allele 2(P=0.056). Only 7.3% of children carried the composite genotype of IL-1A(+4845) and IL-1B(+3954). These results suggest that the polymorphism of IL-1B(+3954) and the positive composite genotype was relatively rare in Korean population.

웅담약침액이 다종의 각막염 유발균에 미치는 항균력에 대한 실험

윤성식,서형식,Yoon, Seong-Sik,Seo, Hyung-Sik 대한약침학회 2010 Journal of pharmacopuncture Vol.13 No.1

Objectives : This experimental study was performed to investigate the effectiveness of Fel Ursi Pharmacopunture solution(FUPS) manufactured by using alcohol/water extraction method. To identify the use of it as eyedrops, antibacterial test on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, and Candida albicans was performed. Methods : After treatment FUPS on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) which cause Keratitis, we investigated anti-bacterial effects of FUPS on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans in terms of measuring MIC and size of inhibition zone respectively. Results : After FUPS was treated, significant changes of MIC on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum and Candida albicans were not observed at all. Conclusions : The present study suggests that FUPS doesn't have anti-bacterial effects on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum and Candida albicans which cause Keratitis. Perhap These results recommend that FUPS doesn't have anti-bacterial effects but have other mechanism which suppress inflammation.

Programmable Color Space Converter 설계에 관한 연구 ( A Study on the Design of Programmable color Space converter )

윤성식,배준석,고학림,박노경,문대철 한국통신학회 1996 한국통신학회 학술대회논문집 Vol.15 No.3

고분자 표면 개질을 통한 Soft Lithography 미세패턴에 관한 연구

윤성식,김동욱,조민호,윤상화,남재도 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.4

가속화 진동내구시험 최적화

윤성식 대한기계학회 2009 기계저널 Vol.49 No.12

제주특별자치도의 성공을 위한 제언

윤성식 한국지방자치학회 2007 한국지방자치학회 학술대회 Vol.2007 No.2

해공간의 매개변수화와 알고리즘을 이용한 BSB 신경망의 설계

윤성식,박주영,박대희 대한전자공학회 1996 전자공학회논문지-B Vol.b33 No.2

This paper proposes a new design technique that can be used for BSB (brain-state-in-a-box) neural networks to realize autoassociative memories. The proposed method is based on the parametrization of solution space and optimization using genetic algorithm. The applicability of the established technique is demonstrated by means of a simulation example, which illustrates its strengths.

BERT를 활용한 상표 의견제출통지서 거절이유 분류모델 개발

윤성식,전재헌,정상일,정찬식,이석준 대한경영정보학회 2021 경영과 정보연구 Vol.40 No.3

멀티미디어 콘텐츠의 증가와 스마트 기기의 보급으로 다양한 종류의 데이터가 폭발적으로 생산되고 있다. 특히, 텍스트 데이터는 오랜 시간 인류의 의사 표현수단이었으며, 텍스트 분석에 대한 수요 및 필요성은 다 양한 분야에서 지속적으로 증가하고 있다. 최근에는 다양한 분야에서 뛰어난 성능을 보이는 딥러닝과 텍스트 를 의미적으로 벡터화하는 워드 임베딩 (word embedding) 방식이 결합된 ELMo (embeddings from language model), GPT (generative pre-training of a language model), BERT (bidirectional encoder representations from transformers)와 같은 모델들이 개발되어왔다. 특히 구글이 개발한 BERT는 현재 자연 어처리 분야에서 가장 뛰어난 성능을 보이는 언어모델로 손꼽히고 있다. 하지만 ‘영어’나 소셜미디어 데이터 와 같은 ‘일반 텍스트’에 특화된 BERT는 ‘한국어’나 ‘R&D 문서, 지식재산권 문서 등’ 전문 분야에 특화된 텍 스트에서 최적의 성능이 구현되지 않기 때문에 전문 분야에 맞는 말뭉치(corpus)를 학습하는 등의 최적화 과 정을 통해 최적의 성능을 도출할 수 있다. 따라서 본 연구에서는 한글 상표 분야에 특화된 BERT를 개발하 기 위해 상표 의견제출통지서 내 거절이유 텍스트 말뭉치를 활용하여 상표 관련 전문 문서에 특화된 토크나 이저 (tokenizer) 모델을 학습하고 이를 BERT에 활용하였다. 제안 모델의 분류 정확도는 기존의 다국어 BERT 모델의 분류 정확도 성능보다 약 4.97%p 높은 96.89%를 기록하였다. 해당 결과를 통해 자연어 분야 에서 높은 성능을 보였던 BERT가 전문 용어로 구성된 말뭉치 데이터에서도 높은 분류 정확도를 보일 수 있 다는 것을 확인하였다. The increase of multimedia contents and the spread of smart devices have resulted in explosive production of various kinds of data. In particular, text data has been a means of expressing human opinion for a long time, and the demand and necessity for text analysis are continuously increasing in various fields. Recently, models such as the ELMo (embeddings from language model), GPT (generating pre-training of a language model), BERT (bidirectional encoder representations from transformation) have been developed that combine deep learning, which is showing excellent performance in various fields, and the word embedding method that semantically vectorizes text. In particular, BERT developed by Google is considered to be one of the most outstanding language models in the field of natural language processing. However, BERT specialized in 'English' and plain text, such as social media data, does not implement optimal performance in specialized texts such as 'Korean' or 'R&D documents, intellectual property documents', so it can achieve optimal performance through optimization such as learning specialized corpus. Therefore, In this study, in order to develop a BERT specialized in the field of Korean trademarks, a tokenizer model specialized in trademark-related professional documents was learned using a text corpus for rejection in the trademark opinion submission notice and used for BERT. The classification accuracy of the proposed model was 96.89%, which is 4.97% higher than the classification accuracy performance of the existing multilingual BERT model. The results showed that BERT, which showed high performance in the field of natural language, could show high classification accuracy even in corpus data composed of specialized terms.

전문대학 식품영양과의 실험실습 및 현장실습 운영실태에 관한 연구

윤성식,소명환,남궁석 한국식품영양학회 1989 韓國食品營養學會誌 Vol.2 No.1

This study was conducted to gather the baseline data on realities of experimental-practice and opinions toward spot-practice, and to examine how to cope with the problems raised at the Department of Food and Nutrition of Junior Technical College in Korea. Ouestionairs were gathered from 42 chairmans of the Department of Food and Nutrition and 140 present dieticians worked in Seoul, Bucheon and Seongnam area. The results are as follows, First, as a whole, each college had 2∼3 experimental laboratory (Lab), in which Cooking Science Lab, Chemistry Lab and Microbiology Lab were occured at higher frequency as Lab's name. Second, the numbers per experimental-practice class were more than 40 at most colleges. 85% of present dieticians have answered to feel too much numbers per the class during their college days, whereas professors' opinions toward the numbers per class were suitable at 20∼30 persons per class. Third, professors' opinion toward the adquate ratio of the theory subject classes to experimental subject classes was suitable at 60 : 40. Dieticians answered to take the theory subject classes partly or mostly on behalf of the experimental-practice classes. Fourth, the main reasons which inhibited normal experimental-practice class were the class for emphasis on examination, the shortage of experiment budget, the excess of class members, the shortage of experimental Lab. Also, this results showed same propensity to present dieticians' opinion toward the same question above. Fifth, among the experimental-practice subjects established at the Department of Food and Nutrition, Diet Therapy Lab was highest frequency class emphasized on theory followed by Nutrition Counselling Lab, Food Processing and Storage Lab, Food Hygiene Lab and Food Microbiology Lab in that order. Here, Basic Chemistry Lab, Biochemistry Lab, Food Microbiology Lab were pointed as subjects far from the present task of dieticians. Sixth, Department of Food and Nutrition, as a whole, has conferred with spot-practice arrangement. About 50% (all who want to join spot-practice) of second year students took part in spot-practice. In the other way, all colleges except for 2 colleges didn't give the credit for the spot-practice system. Seventh, according to the on analysis on spot-practice places, manufacturing company was at highest frequency followed by hospitals, elementary school having group feeding system in that order. Especially, 16.7% (5 colleges) of the total colleges sent the students to the research institute related to food industry for spot-practice experience. Eighth, Professors' opinions toward the spot-practice time and periord were preferable on summer vacation of second year and for 1∼2 weeks, respectively. On the contrary, 74 dieticians answered to the adquate period as for 4 weeks. Ninth, 86 dieticians of the total 140 answered to complete the spot-practice during their college days, which helps the present task of them. Lacks of spot-practice program, Lacks of comprehension of upper personnels and lacks of group feeding equipments ranks higher as difficulties in spot-practice management.

유산균 유전학 및 최근 연구 혁신: 프로바이오틱스와 면역

윤성식,박영서,최학종 한국유산균·프로바이오틱스학회 2013 Current Topic in Lactic Acid Bacteria and Probioti Vol.1 No.1

As for probiotics, that is generally defined as a live cell supplement which is able to bring health benefits to their hosts, even though the concept is still remains in controversy. Scientific works has given positive answers to the questions about if many LAB strains function as probiotic bacteria in human and animals. Currently, the interactions between human and LAB are actively investigated in human intestine. From a biodiversity perspective, this particular subject in human intestine is also extensively explored by a number of researchers worldwide. So far, it has been found that new strains and new genes were discovered in gut microbiota which is reported unique in individual. Complete analysis on gut microbiota is necessary in order to understand the possible beneficial roles of LAB in human intestinal track. When it comes to qualification, a probiotic strain is proved to be safe to consume and healthful to human after it is orally administered. On the other hand, LAB is present as a member of gut microbiota, plays an important role in innate immunity and acquired immunity. Their immune-modulation activity is occurred not only in intestine but also systemic in human body. It is generally accepted that immune function by LAB is not genus-specific but strain-specific. More recently, new mechanism accounting for interactions between host and LAB has been understood after a soluble factor from LAB was found to be directly associated with the epidermal growth factor receptor (EGFR) on intestinal epithelial cells. The soluble factor could be applied in the development of therapeutic agents. In conclusion, future research focus should be placed on the studies on the new LAB factors affecting human immune responses and its desirable effects because many different kinds of fermented foods representing kimchi have survived for a long time and those are popularly consumed in this country.