황련의 증량에 따른 전탕액이 Staphylococcus aureus에 미치는 항균효과에 대한 실험정 연구

서형식 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.3

Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus aureus that induce keratitis. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to 50㎕ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume.

전도산(顚倒散)의 추출용매에 따른 항염 및 항산화 비교 연구

서형식,Seo, Hyung-Sik 대한한방안이비인후피부과학회 2010 한방안이비인후피부과학회지 Vol.23 No.2

Objective : The purpose of this study was to compare anti-Inflammation and anti-oxidation of Jeondo-San(JDS) extracted with two kinds of solvents, ethanol and water. Methods : Two kinds of JDS extractions were prepared 20, 50, $100\;{\mu}g/mg$. The Cytotoxicity was measured by MTT assay in Raw 264.7 cell. The anti-inflammation effects were measured by inhibitory efficacy on $PGE_2$, NO, TNF-$\alpha$, COX-2 and iNOS in Raw 264.7 cell. The anti-oxidation effects were measured by ROS inhibitory efficacy, intracellular GSH synthesis and DPPH Radical scavenging in HaCaT cell. Results : 1. All of JDS extraction groups had no cytotoxicity in Raw 264.7 cell. 2. All of JDS extraction groups showed significantly inhibitory effect on production of $PGE_2$. Inhibitory efficacy increased in accodance with concentration. 3. All of JDS extraction groups showed significantly inhibitory effect on production of NO. Inhibitory efficacy increased in accodance with concentration. 4. All of JDS extraction groups did not show significantly inhibitory effect on production of TNF-$\alpha$. 5. $100\;{\mu}g/ml$ JDS extracted with ethanol and $50\;{\mu}g/ml$, $100\;{\mu}g/ml$ JDS extracted with water showed inhibitory effect on iNOS expression. 6. All of JDS extraction groups showed significantly inhibitory effect on production of ROS. Inhibitory efficacy increased in accodance with concentration. Ethanol extractions were better than water extractions. 7. $100\;{\mu}g/ml$ JDS extracted with ethanol only produced GSH of $32{\pm}5.2%$. 8. All of JDS extraction groups showed significantly scavenging effect of DPPH radicals. Inhibitory efficacy increased in accodance with concentration. Ethanol extractions were better than water extractions. Conclusion : Two kinds of JDS extractions have not cytotoxicity and inhibit production of NO. JDS extracted with water was effective in anti-inflammation, JDS extracted with ethanol was effective in anti-oxidation.

보골지(補骨脂)의 고량주(高梁酒), 소주(燒酒) 추출물이 B16 melanoma 세포주의 멜라닌 합성에 미치는 영향

서형식,Seo, Hyeong-Sik 대한한방안이비인후피부과학회 2008 한방안이비인후피부과학회지 Vol.21 No.1

Objective : This research was carried out to compare the effect of Gorynju and a Soju extract Psoraleae fructus on melanin synthesis of B16 melanoma cells. Methods : To investigate melanin synthesis of B16 melanoma cells, this research was measured cell survival, tyrosinase activity, melanin synthesis, western blot. Results : Both Gorynju and Soju extract Psoraleae fructus, cell toxicity depended on the density. Tyrosinase activity depended on the density of Gorynju extract Psoraleae fructus and statistic was showed significant(0.5, 1, 2, 3 ${\mu}g/ml$), in a Soju extract Psoraleae fructus, 1 ${\mu}g/ml$ were showed significant. Melanin synthesis was showed significant in a Soju extract Psoraleae fructus(3, 4 ${\mu}g/ml$). Western blot was showed to depend on the density of Gorynju and a Soju extract Psoraleae fructus. Conclusions : In a tyrosinase activity and a melanin synthesis, the intermediate alcohol of Gorynju and a Soju may be suitable to use.

소염 약침액, 황련해독탕, 황련이 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구

서형식,Seo, Hyung-Sik 대한한방안이비인후피부과학회 2006 한방안이비인후피부과학회지 Vol.19 No.2

Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma on Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50{\mu}l$ according to density Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus epidermidis in Soyum Herbal-acupuncture dippear all sample. 2. MIC on Staphylococcus epidermidis in Hwangryunhaedoktang disappear all sample. 3. MIC on Staphylococcus epidermidis in Coptidis rhizoma was 100%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The present author think that Soyum Herbal-acupuncture must be extracted a new method for anti-bacterial potency and Coptidis rhizoma was made Herbal-acupuncture for eye drops.

소풍도적탕(消風導赤湯)과 사물소풍산(四物消風散)의 항염 및 항산화 효과에 대한 실험적 연구

서형식,Seo, Hyung-Sik 대한약침학회 2010 Journal of pharmacopuncture Vol.13 No.1

Objective: This experimental study was performed to investigate the anti-inflammation and anti-oxidant effects of Sopungdojeok-tang(SPDJT) and Samulsopung-san(SMSPS) which were used to treat patient with atopic dermatitis Methods: Anti-inflammation and anti-oxidant effects of SPDJT and SMSPS were measured by the inhibitory ability of Nitric oxide(NO) production and the scavenging for 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical. Results: 1. SPDJT and SMSPS were not showed cell toxicity. 2. In inhibitory effects against NO production, all groups of SPDJT were showed anti-inflammation, but all groups of SMSPS were not showed anti-inflammation. There were statistical significances between $20{\mu}g/m\ell$ and 4, $10{\mu}g/m\ell$ in SPDJT groups. 3. In DPPH radical scavenging activity, all groups of SPDJT and all groups of SMSPS were showed anti-oxidant effects. There were statistical significances between $20{\mu}g/m\ell$ SPDJT group and 4, $10{\mu}g/m\ell$ SMSPS groups. Conclusion: These results suggest that the SPDJT groups are better than the SMSPS groups in antiinflammation and anti-oxidant effects.

황련의 증량에 따른 전탕액이 Pseudomonas aeruginosa에 미치는 항균효과에 대한 실험적 연구

서형식,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.1

Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Pseudomonas aeruginosa that induce keratitis. Methods : MIC was measured by dropping to 50 ${\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 120g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : MIC on Pseudomonas aeruginosa in Coptidis rhizoma extract compared with quantity(40g, 80g, 120g, 160g) was not showed all samples(100%, 10%, 1%, 0.1%). Conclusions : Coptidis rhizoma extract compared with quantity was not showed anti-bacterial potency on Pseudomonas aeruginosa. The results differ that Coptidis rhizoma extract was showed anti-bacterial potency in 1999 year. Anti-bacterial Potency of Coptidis rhizoma extract compared with quantity on Pseudomonas aeruginosa must be studied.

An Experimental Study of the Anti-oxidant and the Anti-inflammatory Effects of Alum and Burnt Alum

서형식 대한약침학회 2012 Journal of pharmacopuncture Vol.15 No.2

Objectives: The purpose of this study was to compare the antioxidant and anti-inflammatory effects of Alum (AL) and Burnt Alum (BAL), which are commonly used as external ointments. Methods: Extracts of AL and BAL were classified into three groups: 20, 50, and 100 ㎎/㎕. The cytotoxicity was measured by using MTT assays in human keratinocyte cell line (HaCaT). The anti-oxidant effect was measured by using the DPPH (1,1-diphenyl-2-picryl-hydrazyl-hydrate) radical scavenger. The anti-inflammatory effect was measured by using the inhibitory efficacy for the amount of nitric-oxide (NO) produced in mouse macrophage cell line (RAW 264.7). Results: BAL showed a higher level of cytotoxicity than AL. The AL groups showed a concentration-dependent scavenging effect on DPPH radicals, but no significant relevance was found. The BAL groups showed a concentration-dependent scavenging effect on DPPH radicals. The scavenging effects of the BAL groups were almost insignificant, but the values for the 20, 50, and 100 ㎍/㎖ trials were different. The BAL groups showed significant concentration-dependent inhibitory effects on NO production, but the AL groups did not. Conclusions: AL showed an anti-oxidant effect more efficiently than BAL did, which demonstrated a superior anti-inflammatory effect. Therefore, for external usage, AL must be distinguished from BAL.

황련의 증량에 따른 전탕액이 Staphylococcus aureus에 미치는 항균효과에 대한 실험적 연구

서형식,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.3

Objective This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus aureus that induce keratitis. Methods Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was showed anti-bacterial potency compared with quantity and density in 100% and 10% of all samples(40g, 80g, 160g). 2. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. 3. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples ($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. Anti-bacterial potency of 80g Coptidis rhizoma extract decreased compared with 40g. Anti-bacterial potency of 160g Coptidis rhizoma extract decreased compared with 40g in $20{\mu}l,\;30{\mu}l$. Conclusions Coptidis rhizoma extract was showed anti-bacterial potency compare with quantity and density. In herbal drug, antibacterial potency compare with quantity and density must be studied.

탕포산(湯泡散)이 Staphylococcus species(S. aureus, S. epidermidis)의 배양일에 따른 항균효과의 지속성에 관한 연구

서형식,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.2

Objectives : This experimental study was performed to investigate the continuous anti-bacterial potency of Tangpo-san on cultivation of Staphylococcus species(S. aureus, S. epidermidis)that induce eye disease. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to 50 ${\mu}$l diluted Tangpo-san(100%, 10%, 1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. Anti-bacterial potency was measured by the size of inhibition zone with change of volume(20 ${\mu}$l,30 ${\mu}$l,40 ${\mu}$l,50 ${\mu}$l). Results : 1. Anti-bacterial potency of Tanpo-san on S. aureus was not appeared all samples. Anti-bacterial potency with change of volume was increased in propotion to increase volume, and the Anti-bacterial potency of 2 days was equal to 6 days. Anti-bacterial potency of Tangpo-san on S. aureus was appeared continuous. 2. Anti-bacterial potency of Tangpo-san on S. epidermidis was appeared in 100%, 10% on 2 and 6 days, and the Anti-bacterial potency of 6 days was decreased. In 2 days, Anti-bacterial potency was appeared 40 and 50u1, in 6 days, Anti-bacterial potency was appeared all samples. Anti-bacterial potency with change of volume was increased in propotion to increase volume and increased on 6 days, but bacteria was increased. Anti-bacterial potency of Tangpo-san on S. epidermidis wasn't appeared continuous. Conclusions : Anti-bacterial potency of Tangpo-san on cultivation of S. aureus showed continuous, but on cultivation of S. epidermidis was not showed continuous.

점안용 웅담약침액의 안점막자극실험

서형식,Seo, Hyeong-Sik 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.3

Objective: This study was done to investigate the safety of Fel Ursi pharmacopuncture solution manufactured with freezing dryness method to use eye drop. Methods: The eye irritation test of this material was performed according to the Regulation of Korea Food & Drug Administration(2005. 10. 21, KFDA 2005-60). After Fel Ursi pharmacopuncture solution was medicated in the left eye of the rabbits, the author observed eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Results: 1. After Fel Ursi pharmacopuncture solution was medicated in the left eye of the rabbits, there wasn't physical problem at 9 rabbits. 2. After Fel Ursi pharmacopuncture solution was medicated in the left eye of the rabbits, there wasn't eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Conclusions: I suggested that Fel Ursi pharmacopuncture solution didn't induced eye irritation in rabbits.