정신분열병과 N-Methyl-D-Aspartate 수용체 유전자와 연관된 VNTR 표지자인 D9S158 다형성의 연합

김종원,채영규,정은기,김길숙,박강규,이영호,최인근,양병환,한진희 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

정신분열병과 NMDA 수용체와 연관된 D9S158 유전적 표지자와의 연관성을 알아보기 위해 75명의 정신분열병 환자군과 87명의 정상대조군을 대상으로 중합효소연쇄반응을 이용하여 연합연구를 시행하여 다음과 같은 결론을 얻었다. D9S158 유전좌위에는 모두 8개의 대립유전자가 있었고, 각 대립유전자는 cytosine-adenine염기가 반복되는 217염기쌍에서 233염기쌍의 형태였다. 먼저 정신분열병 환자와 정상대조군의 대립유전자의 빈도를 비교해 본 결과 통계적으로 유의한 차이를 보이지 않았다(p〉.05). 환자군을 좀 더 동질적으로 만들기 위해 정신분열병의 가족력 유무, 음성 및 양성증상, 연성신경학적 징후, DSM-IV 임상아형에 따라 환자군을 나누어 대립유전자의 빈도를 비교해 본 결과, 특정 임상양상과 관련이 있는 대립유전자를 발견하지 못하였다. 결론적으로 본 연구에서는 D9S158 유전좌위와 정신분열병의 연관성을 입증하지 못하였으나 NMDA 수용체 유전자주위의 다른 표지자나 좀 더 유전적으로 동질한 환자군을 대상으로 한 추후연구가 필요할 것으로 생각된다. An association study with Korean schizophrenic patients(N=75) and normal controls(N=87) was performed to find the relationship between D9S158 polymorphism and schizophrenia using polymerase chain reaction. Eight different alleles of a dinucleotide polymorphism on D9S158 locus were observed in both group. When we compared the frequencies of alleles between schizophrenics and normal controls, there was no significant difference between two groups. To increase homogeneity of schizophrenic group, we divided schizophrenic group by clinical phenotypes such as family history, negative and positive symptoms(PANSS), soft neurologic signs(NES-K) and DSM-IV diagnostic subtypes. Then we compared the frequencies of alleles among subgroups of clinical phenotypes, and there were no significant differences between subgroups(p〉.05). Although our findings fail to provide an evidence of association between schizophrenia and D9S158 locus, further investigation of other loci that are linked to NMDA receptor gene may be needed in genetically homogeneous subgroups of schizophrenia.

CoMFA 법을 이용한 3-아릴이소퀴놀린 화합물들의 SK-OV-3 암세포에 대한 가상의 약물 작용 수용체 해석

김의기,민선영,정병호,천승훈,최보길,조원제 전남대학교 약품개발연구소 2002 약품개발연구지 Vol.11 No.-

We have performed a 3D-QSAR/ CoMFA analysis of the cytotoxiv activities of thirty-five 3-arylisoquinoline derivative against SK-OV-3 tumor cell line. The results suggested that the electrostatic, steric and hydrophobic factors of 3-arylisoquinoline were strongly correlated with the antitumor activity. Considerable predictive ability (cross-validated r2 as high as 0.841) was obtained through CoMFA.

우황청심원(牛黃淸心元)이 저산소증 유발 배양신경세포에 미치는 영향

조규선,신길조,문일수,이원철,정승현,Cho, Gyu-Seon,Shin, Gil-Cho,Moon, Il-Soo,Lee, Won-Chul,Chung, Sung-Hyun 대한한방내과학회 2001 大韓韓方內科學會誌 Vol.22 No.2

Objectives : The purpose of this investigation is to evaluate the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast. Methods : To evaluate the effect of Woohwangcheongsim-won on neuronal death caused by hypoxia, the survival rate of E18 cortical neuroblast was measured with MTT assay and the changes of several synaptic proteins and enzymes were investigated with the immunoblot assays. Results : The E18 cortical neuroblasts were added 50, 100, 500, 1,000, and $5,000{\mu}g/ml$ Woohwangcheongsim-won. They showed neurotoxicity, when the concentration of Woohwangcheongsim-won was above $1,000{\mu}g/ml$. The E18 cortical neuroblasts, which were added 50, 100, and $500{\mu}g/ml$ Woohwangcheongsim-won, were exposed 98% $N_2/5%\;CO_2$ for 3 hours to induce hypoxia, 3 days later, the survival rate of $50{\mu}g/ml$ Woohwangcheongsim-won was 141.5% when compared to the control group. On the immuneblot assays, the expressions of ${\alpha}$CaMKII, NR2A, NR28, PDE2, PSD-95, and eEF-$1{\alpha}$ were increased in normoxia, but those of NR2A, NR2B were decreased in hypoxia when compared to the control group. Conclusions : The data shows that the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast is a significant result.

Effect of Extrinsic Factors on Differentiated Cardiomyocyte-like Cells from Human Embryonic Stem Cells

Gil, Chang-Hyun,Jang, Jae-Woo,Lee, Won-Young,Park, Ze-Won,Lee, Jae-Ho,Chung, Sun-Hwa,Chae, Jung-Il,Chung, Hyung-Min The Korean Society of Animal Reproduction 2009 Reproductive & developmental biology Vol.33 No.4

Cardiovascular diseases (CVDs) are one of the most cause of death around the world and fields of interest for cardiac stem cells. Also, current use of terminally differentiated adult cardiomyocytes for CVDs has limited regenerative capacity therefore any significant cell loss may result in the development of progressive heart failure. Human embryonic stem cells (hESCs) derived from blastocyst-stage embryos spontaneously have ability to differentiate via embryo-like aggregates (endoderm, ectoderm and mesoderm) in vitro into various cell types including cardiomyocyte. However, most effective molecule or optimized condition which can induce cardiac differentiation of hESCs is rarely studied. In this study, we developed both spontaneous and inductive cardiomyocyte-like cells differentiation from hESCs by treatment of induced-factors, 5-azacytidine, BMP-4 and cardiogenol C. On the one hand, spontaneous and inductive cardiomyocyte-like cells showed that cardiac markers are expressed for further analysis by RT-PCR and immunocytochemistry. Interestingly, BMP-4 greatly improved homogeneous population of the cardiomyocyte-like cells from hESCs CHA15 and H09. In conclusion, we verified that spontaneously differentiated cells showed cardiac specific markers which characterize cardiac cells, treated extrinsic factors can manage cellular signals and found that hESCs can undergo differentiation into cardiomyocytes better than spontaneous group. This finding offers an insight into the inductive factor of differentiated cardiomyocytes and provides some helpful information that may offer the potential of cardiomyocytes derived from hESCs using extrinsic factors.

Original Articles : Clinical impacts of hazardous alcohol use and obesity on the outcome of entecavir therapy in treatment-naive patients with chronic hepatitis Binfection

( Won Gil Chung ),( Hong Joo Kim ),( Young Gil Choe ),( Hyo Sun Seok ),( Chang Wook Chon ),( Yong Kyun Cho ),( Byung Ik Kim ),( Young Yool Koh ) 대한간학회 2012 Clinical and Molecular Hepatology(대한간학회지) Vol.18 No.2

Background/Aims: The aim of this study was to analyze the clinical impacts of obesity and hazardous alcohol use on the outcome of entecavir (ETV) therapy in chronic hepatitis B (CHB) patients. Methods: The medical records of 88 treatment-naive patients who were diagnosed with CHB and received ETV between March 2007 and September 2009 were analyzed retrospectively. Body mass index (BMI) values and Alcohol Use Disorders Identifi cation Test (AUDIT) scores were obtained at 6 months after the initiation of ETV (0.5 mg daily) treatment. Results: A BMI of 25 kg/m2 or more was recognized as an indicator of obesity, and a total AUDIT score of 8 or more was recognized as an indicator of hazardous alcohol use. Of the cohort, 24 patients (27.3%) were obese and 17 (19.3%) were hazardous alcohol users. The rate of seroconversion, alanine aminotransferase (ALT) normalization, and hepatitis B virus (HBV)-DNA negativity (<300 copies/mL) at 3, 6, and 12 months of treatment did not differ significantly between the normal-BMI and high-BMI groups. Moreover, the rate of seroconversion and HBV-DNA negativity at 3, 6, and 12 months of treatment did not diff er signifi cantly between the nonhazardous and hazardous alcohol users. However, the frequency of ALT normalization at 12 months was signifi cantly lower among hazardous alcohol users (91.5% vs. 70.6%; P=0.033). Conclusions: Obesity and hazardous alcohol drinking have no significant impact on the outcome of ETV treatment. However, the ALT normalization rate at 12 months after initiation of ETV treatment was signifi cantly lower among the hazardous alcohol users. (Clin Mol Hepatol 2012;18:195-202)

Clinical impacts of hazardous alcohol use and obesity on the outcome of entecavir therapy in treatment-naïve patients with chronic hepatitis B infection

Won Gil Chung,Hong Joo Kim,Young Gil Choe,Hyo Sun Seok,Chang Wook Chon,Yong Kyun Cho,Byung Ik Kim,Young Yool Koh 대한간학회 2012 Clinical and Molecular Hepatology(대한간학회지) Vol.18 No.2

Background/Aims: The aim of this study was to analyze the clinical impacts of obesity and hazardous alcohol use on the outcome of entecavir (ETV) therapy in chronic hepatitis B (CHB) patients. Methods: The medical records of 88 treatment-naïve patients who were diagnosed with CHB and received ETV between March 2007 and September 2009 were analyzed retrospectively. Body mass index (BMI) values and Alcohol Use Disorders Identifi cation Test (AUDIT) scores were obtained at 6 months after the initiation of ETV (0.5 mg daily) treatment. Results: A BMI of 25 kg/m2 or more was recognized as an indicator of obesity, and a total AUDIT score of 8 or more was recognized as an indicator of hazardous alcohol use. Of the cohort, 24 patients (27.3%) were obese and 17 (19.3%) were hazardous alcohol users. The rate of seroconversion, alanine aminotransferase (ALT) normalization, and hepatitis B virus (HBV)-DNA negativity (<300 copies/mL) at 3, 6, and 12 months of treatment did not differ significantly between the normal-BMI and high-BMI groups. Moreover, the rate of seroconversion and HBV-DNA negativity at 3, 6, and 12 months of treatment did not diff er signifi cantly between the nonhazardous and hazardous alcohol users. However, the frequency of ALT normalization at 12 months was signifi cantly lower among hazardous alcohol users (91.5% vs. 70.6%; P =0.033). Conclusions: Obesity and hazardous alcohol drinking have no significant impact on the outcome of ETV treatment. However, the ALT normalization rate at 12 months after initiation of ETV treatment was signifi cantly lower among the hazardous alcohol users. (Clin Mol Hepatol 2012;18:195-202) Background/Aims: The aim of this study was to analyze the clinical impacts of obesity and hazardous alcohol use on the outcome of entecavir (ETV) therapy in chronic hepatitis B (CHB) patients. Methods: The medical records of 88 treatment-naïve patients who were diagnosed with CHB and received ETV between March 2007 and September 2009 were analyzed retrospectively. Body mass index (BMI) values and Alcohol Use Disorders Identifi cation Test (AUDIT) scores were obtained at 6 months after the initiation of ETV (0.5 mg daily) treatment. Results: A BMI of 25 kg/m2 or more was recognized as an indicator of obesity, and a total AUDIT score of 8 or more was recognized as an indicator of hazardous alcohol use. Of the cohort, 24 patients (27.3%) were obese and 17 (19.3%) were hazardous alcohol users. The rate of seroconversion, alanine aminotransferase (ALT) normalization, and hepatitis B virus (HBV)-DNA negativity (<300 copies/mL) at 3, 6, and 12 months of treatment did not differ significantly between the normal-BMI and high-BMI groups. Moreover, the rate of seroconversion and HBV-DNA negativity at 3, 6, and 12 months of treatment did not diff er signifi cantly between the nonhazardous and hazardous alcohol users. However, the frequency of ALT normalization at 12 months was signifi cantly lower among hazardous alcohol users (91.5% vs. 70.6%; P =0.033). Conclusions: Obesity and hazardous alcohol drinking have no significant impact on the outcome of ETV treatment. However, the ALT normalization rate at 12 months after initiation of ETV treatment was signifi cantly lower among the hazardous alcohol users. (Clin Mol Hepatol 2012;18:195-202)

류마티스관절염 환자로부터 분리한 윤활막섬유모세포의 TRALL에 대한 세포사멸 반응 및 TRAIL 수용체 발현 양상에 대한 조사

조원길(Won-Gil Cho),임현성(Hyun-Sung Leem),백두진(Doo-Jin Paik),정호삼(Ho-Sam Chung),김원규(Won-Kyu Kim),최충혁(Chung-Hyuk Choil),윤지희(Jeehee Youn) 대한체질인류학회 2001 해부·생물인류학 (Anat Biol Anthropol) Vol.14 No.3

류마티스관절염은 염증성 자가면역 질환의 일종으로 윤활막이 증식(hyperplasia)되는 소견을 나타내며, 이는 일부 형질전환된 특성을 지닌 윤활막세포에서 apoptosis의 장애가 일어나는 경과로 알려졌다. TNF-related apoptosis inducing ligand (TRAIL)은 TNF ligand superfamily의 일원으로, 두 수용체(TRAILR-l과 2)를 통해 세포사영 신호를 세포 내로 전달한다. 반면 두 decoy 수용체인 TRAILR-3와 4는 TRAIL 유도 세포사멸 경로를 방해한다. 류마티스관절염 판자의 윤활악서 1 포가 TRAIL 에 의하여 세포사영 반응이 일어나는지 조사하기 위하 여, 각각 두 명의 류마티스관절염 환자와 골관정염 환자로부터 분리 애양한 윤활약섬유오세포주들은 human TRAIL호 자극한 후 Mteassay를 실행하였다. 두 류마티스관정염 세포들은 TRAIL 에 반응하여 세포 생존융이 강소한 반면 , 골관정염 세포들은 TRAIL에 우반응을 보이거나 류마티스관정엽 세포에 비해 현저히 감소된 민강 성율 보였다 RT-PCT 과 연역조직화학적 분켜 결과 , TRAILR-I, -2 , -3의 발현 양상은 모든 세포주에서 유사했 으나 , TARILR-4 의 발현 정도는 류마티스 관절엽 세포에서 골판절엽 세포에 비해 강소한 경과를 보였다 그러므로, 이상의 결과들은 윤활막섬유모세포는 류마티스관절염 진행 과정 중 TRAIL을 통한 세포사멸에 대하여 민감성을 획득하며, 이런 세포사멸 신호는 TRAILR-4 발현의 조정에 일부 의존적일 것 2. 로 추정된다

전처리과정을 거친 ISS 추적데이터를 이용한 궤도결정

김원길(Won-Gil Kim),이준호(Junho Lee),이병선(Byoung-Sun Lee),정대원(Dae-Won Chung) 한국항공우주학회 2017 한국항공우주학회 학술발표회 논문집 Vol.2017 No.4

이중풍선 소장내시경으로 진단한 공장 게실 출혈: 증례 보고와 문헌 고찰

정원길 ( Won Gil Chung ),김홍주 ( Hong Joo Kim ),김석 ( Seok Kim ),최영길 ( Young Ghil Choi ),석효선 ( Hyo Seon Seok ),이도영 ( Do Young Lee ),박정호 ( Jung Ho Park ),김승권 ( Seong Gwun Kim ) 대한소화기학회 2007 대한소화기학회지 Vol.50 No.4

Jejunal diverticula is a rare disease and an unusual cause of obscure gastrointestinal hemorrhage. Obscure gastrointestinal bleeding is difficult to treat because the bleeding site cannot be identified by routine endoscopy and contrast studies. A wireless capsule endoscopy is not invasive and can visualize the entire small bowel. However, this method has limitations of incapability of taking biopsies and performing endoscopic interventions such as polypectomy or stent insertion. The double-balloon enteroscopy is being used frequently for the diagnosis and management of various small bowel diseases. We report a case of proximal jejunal diverticular bleeding diagnosed by double-balloon enteroscopy and treated with angiographic embolization. (Korean J Gastroenterol 2007;50:260-264)

Effect of Extrinsic Factors on Differentiated Cardiomyocyte-like Cells from Human Embryonic Stem Cells

Chang-Hyun Gil,Jae-Woo Jang,Won-Young Lee,Ze-Won Park,Jae-Ho Lee,Sun-Hwa Chung,Jung-Il Chae,Hyung-Min Chung 한국동물번식학회 2009 Reproductive & developmental biology Vol.33 No.4