Analysis of Genetic Diversity of Korean Wheat Cultivars Using Microsatellite DNA Polymorphisms

Park,Yong-Jin,Cho,Gyu-Taek,Ma,Kyung-Ho,Lee,Sok-Young,Lee,Jung-Ro,Kim,Young-Chang,Cho,Eun-Gi,Kim Chang-Yung,Nam,Jung-Hyun,Rao,V,Ramanatha,Kang,Hee-Kyoung 한국자원식물학회 2004 Plant Resources Vol.7 No.2

Genetic background and phylogenetic relationships among 20 Korean wheat cultivars were assessed using microsatellites after amplifying with 13 SSR primer pairs. Average allele number per primer pair was 3.36. Genetic similarities for every pair of cultivars ranged from 0.42 to 0.97, with 0.69 of overall average. Korean cultivars were divided into two major groups based on microsatellite DNA polymorphisms. Group I consisted of relatively old cultivars developed until 1970s, and group II contained the recent cultivars developed during 1980s and 1990s. Amongst old elite cultivars/lines, ‘Yukseung 3’, ‘Norin 12’ and ‘Norin 72’ contributed most to the genetic background of cultivars belonging to group I, and ‘Norin 4’, ‘Norin 12’, ‘Norin 43’ and ‘Norin 72’ to group II, respectively. The phylogenetic relationship of Korean wheat cultivars was in accordance with the genealogical data of each cultivar. The genetic background of each cultivar was assessed from the point of breeding and germplasm management such as variety identification and duplicated accessions for assisting in developing a system for the registration of new variety based on the molecular characterization in future.

금호강 유역 산림의 경관생태적 패턴분석

박경훈,정성관,이현택,오정학,김경태 한국지리정보학회 2004 한국지리정보학회지 Vol.7 No.3

산림의 단편화와 같은 인위적 교란은 유역생태계의 지속성에 영향을 미치기 때문에, 본 연구는 각종 개발사업에 따른 산림의 단편화를 사전에 예방하기 위해서 경관지수와 GIS를 이용하여 금호강 유역에 분포하는 산림구조를 정략화하였다. 소유역 단위의 경관지수값은 인자분석에 의해 전체분산을 또% 정도 설명하는 3개의 공통인자를 단순화하였다. 산림단편화 정도는 소유역별 인자점수를 토대로 계산하였고, 이는 도시지역 면적률(r=0.827, p<0.01, R2=0.685), 고도(r=-0.637, p<0.01)그리고 경사도(r=-0.593, p<0.01)와 상호관련성이 있는 것으로 나타났다. 이상과 같이 본 연구는 금호강 유역관리를 위한 산림경관의 건전성 및 구조적 패턴을 평가하기 위한 방법론과 경관지수값을 제시하였다. 향후에는 경관패턴 및 그 변화가 유역환경의 건전성에 미치는 영향을 규명하기 위한연구가 필요할 것이다. An artificial disturbance like fragmentation in watershed forest has impacted a sustainability of watershed ecosystem, therefore this research quantified the landscape structure in the Geumho river watershed using landscape indices and GIS. Landscape indices were calculated from the forest distributed maps for 24 subwatersheds. Three common factors, which explained about 85% of the variation in the original data, were extracted by a factor analysis. The fragmentation gradient in forest landscape, which was calculated from the factor scores, was correlated with proportion of urban land (rte. 827, p<0.01, R²=0.685). elevation (r=-0.637, p<0. 01) and slope gradient (r=-0.593, p<0. 01) . The result of the study presented that the methodology and the values of landscape indices to assess the structural patterns of forest landscape for the Geumho river watershed management. Future research will be directed towards the detection of impacts of landscape patterns and their changes on the integrity of watershed environments.

사상체질과 신체형태지수와의 연관성

박경아,김희진,허경석,안수경,이성호,이종은,이원택 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.4

음향방사로 인한 주기구조물에의 모우드 해석

박상기,임경택,이동하,김택현,김종태 朝鮮大學校 機械技術硏究所 1999 機械技術硏究 Vol.2 No.2

In this study, the mode shape of infinite fluid loaded plate is examined. The stiffeners are assumed to be equally spaced and exert only force on the Plate. The response of a fluid loaded ㄷ-type stiffened plate to a convected harmonic pressure and the far field radiation Produced by a point force excitation have been found by using Fourier transforms, a method which is particularly useful when fluid loading is included. At low and high frequencies the far field Pressure for a point excited stiffened plate is dominated by the fluid properties and the properties of the plate at the point of excitation. Mode in the far field pressure is found at the given direction of scattering frequencies, and in these frequencies the stationary phase wave frequencies are found to coincide with the free wave propagation constants for the stiffened plate. Also, the variation in the modes of the sound pressure level is increased with the increases of the bay space and in the bay area

타리비드 정(오플록사신 100㎎)에 대한 파비드 정의 생물학적동등성

박완수,조성희,이헌우,임호택,홍성제,서성훈,류재환,이경태 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.1

The purpose of the present study was designed to evaluate the bioequivalence of two ofloxacin tablets, Tarivid (Jell Pharm. Co., Ltd.) and Favid (ILHWA Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). Twenty-four normal male volunteers, 23.67±3.12 year in age and 68.50±7.23 kg in body weight, were divided into two groups and a randomized 2x2 cross-over study was employed. After four tablets containing 100 mg of ofloxacin were orally administered, blood was taken at predetermined time intervals and concentrations of ofloxacin in plasma were deter mined using HPLC. Pharmacokinetic parameters such as AUC_(t) and T_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t), and C_(max) and untransformed T_(max). There were no sequence effects between two formulations in these parameters. The 90% confidence intervals for the log transformed data were acceptance range of log 0.8 to log 1.25 (e.g., log 0.94-log 1.04 and log 0.90-log 1.07 for AUC_(t) and C_(max) respectively). The major parameters. AUC_(t) and C_(max) met the criteria of KDFA for bioequivalence indicating that Favid tablet is bioequivalent to Tarivid tablet.

난소암 환자 복수내의 면역복합체로부터 새로운 암관련항원 존재 확인

노만택,박장환,정용훈,김정목,김경태,조양자 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.2

Immune complexes were isolated from ascitic fluid of patients with ovarian carcinoma by using protein-A Sepharose CL-4B affinity chromatography, and antigen-antibody complexes then dissociated by ion exchange chromatography in the presence of 8M urea. Only one antigen fraction was obtained by the ion exchange chromatography, but three antigenic bands were demonstrated by SDS-PAGE. The molecular weights of the three protein bands were 70, 41, and 38 kDa, respectively. Of the three proteins the 38 kDa band has already been known, but 70 and 41 kDa bands seems to represent new ovarian tumorassociated antigens. This study may suggest the involvement of multiple antigens in ovarial tumor which can be included in producing tumor-directed monoclonal antibodies

熱環境의 理論的 背景에 관한 硏究

林晩澤,朴京甲,鄭圭福,鄭碩熙 조선대학교 국토개발연구소 1993 국토개발연구 Vol.13 No.1

The design ranges of human-environmental system can be divided into seven kinds, water, thermal, air, light, acoustic, color, psychology and environment. This study aims at clarifying the theoretical background of thermal environment is what point of view is historically. Thermal environment developed based on the thermal comfort index, standard and law establishment etc. To constitute the amenity of thermal environment and to control it, it needs to prediction and adaptability.

유색 보리 종실의 품종별 색소 특성

주완택,민경수,박노동 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

유색보리 4품종과 보통보리 4품종 종실의 종피에 함유되어 있는 색소의 특성을 분석하여 해당작물의 신품종 육성시 우수계통 선발에 유용한 자료를 제공코자 하였다. 유색(검정)쌀보리인 Ab2231을 표준시료로 하여 실험한 결과 에탄올의 농도가 80%일 때 색소추출 효율이 양호하였다. 80% 에탄올에 1.0% 염산을 첨가한 것이 추출효율이 가장 좋았다. 추출시간에 따른 추출효율성을 조사한 결과 1시간 추출이면 충분하였다. 추출된 색소의 안정성을 유지하기 위하여 첨가시키는 산의 적정농도를 알아본 결과 1.0% HCl을 첨가한 농도에서 가장 낮은 색소량 감소율이 나타났다. 각 보리 품종 시료에서 추출한 색소의 흡광스펙트럼을 조사하였던 바, Ab2231의 그것은 535㎚에서 최대흡광파장을 나타내어 안토시아닌계 색소로 판단되었으며, 호계4호의 그것은 안토시아닌계 이외의 색소를 주로 함유하는 것으로 보였다. 한편, 추출된 색소의 특성에 따라 유색보리의 품종은 Ab2231와 모찌무기로 대표되는 쌀보리 그룹과 Lion과 호계4호로 대표되는 겉보리 그룹으로 분류되었다. The characteristics of the pigments extracted from grains of the colored barley varieties Ab2231. Mozzimugi, Lion, and Hogye 4 were studied. Extraction of the pigments from powder (80 mesh) of the barley grains was most efficient in 80% ethanol containing 1.0% HCI for 1 hr at 60℃. The pigments were also stable in 80% ethanol containing 1.0% HCI. Depending on the absorption spectra, maximal peak wavelength, and the ratio of A_(440)/Anu_(max), the varieties were separated into two groups, colored naked barley (Ab2231 and Mozzimugi) and colored covered barley (Lion and Hogye 4).

조기 및 붕장어를 동결건조한 어육분말의 제조 및 저장안정성

양승택,박경희 慶星大學校 1998 論文集 Vol.19 No.2

This study was designed to make fish meat powder which retains the fish jelly forming ability of fresh fish meat from fish meat paste of yellow corvenia (Pseudoscinea manchuria) and conger eel (Astroconger myriaster). Fish meat powder put into 0.1 mm polyethylene bags and cans as package and checked the keeping qualities such as chemical composition, soluble nitrogen, relative viscosity, water holding capacity (WHC), emulsifying stability (ES) and fish jelly forming ability for 6 months storage at 5℃, 20℃ and 35℃. Crude proteins very predominant in yellow corvenia and conger eel meat powder showing 68.4% and 60.9%, respectively, The freeze-dried powder had good fish jelly forming ability. Dried powder gradually decreased protein solubility, relative viscosity, WHC, ES, and fish jelly forming ability for 6 months storage. Qualities retention of the powder of high temperature sorage was less than those of low temperature storage. Fish jelly forming abilities of the powder were maintained for 6 months and 5 months and 2 months at 5℃ and 20℃ in yellow corvenia, respectively, and those of the powder were 3 months and 2 months at 5℃ and 20℃ in conger eel, respctively. But both samples have been lost fish jully forming ability after 1 month at 35℃. Qualities of the powder packed in cans were a little better than those of polyethylene bags. In the conclusion, freeze-dried powder of yellow corvenia and conger eel neat was very useful for the proparation of fish jelly products.

세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.