흡착제를 이용한 수중의 미량이취물질 분석법 개발에 관한 연구

심은기,김은호,곽철,황영기 경남대학교 신소재연구소 1999 論文集 Vol.11 No.-

본 연구에서는 다양한 흡착제들과 추출용매들을 사용하여 이취물질의 효율적인 분석기술을 개발코자 하였다. Geosmin의 흡착능 실험에서는 XAD 수지계통이 월등히 우수한 흡착능을 가진 것으로 나타났으며, 이중에 XAD-2 수지는 시료 100ml 당 300mg을 투여하였을 때 Geosmin 흡착능이 가장 뛰어났다. 추출용매별 Geosmin의 추출능 실험에서 액/액 추출법으로 실험하였을 때 MTBE와 Dichloromethane은 48%, 47%로 거의 비슷한 회수율을 나타내었고, XAD-2 수지를 이용한 고/액추출법으 경우에는 MTBE가 94% 정도로 회수율이 가장 높았고, Dichloromethane 79%, n-Hexane 39%, Diethylether 35% 순으로 나타났다. 염석제(NaCl)를 첨가하였을 경우에 Geosmin의 회수율 실험에서 MTBE를 이용하여 액/액 추출법으로 실험하였을 때 염석제 5g 첨가시 회수율이 75%로 가장 높았고, XAD-2 수지를 사용한 고/액추출법의 경우에는 추출용매 MTBE와 Dichloromethane에 대해 염석제의 투여에 의한 회수율의 증가는 없는 것으로 나타났다. XAD-2 수지의 진탕시간별 Geosmin의 흡착실험에서는 24시간 동안에 Geosmin에 대한 흡착량에는 거의 큰 변화를 나타내지 않았다. 최적의 흡착제는 XAD-2 수지이고 용매는 MTBE가 가장 안정적이고 우수한 것으로 평가되었으며, 액/액 추출법의 경우에 염석제를 첨가하였을 때가 첨가하지 않은경우 보다 회수율이 높게 나타났다. 고상 추출시 분석감도도 용매만 사용하여 추출한 결과보다 2배 가까이 높게 나타나 극미량 함유물질 분석시 전처리 기술로 응용 가능한 것으로 평가되었다. Even if odorous compounds remain very low concentration in water. it cause strong odor. Because Geosmin and most of odorous compound had very low vaporization. those were difficult to analyze with GC/MSD and Purge & Trap. So. we needed pre-treatment method for decreasing amounts of extracting solvents, improving recovery efficiencies and increasing analytical efficiencies. This study developed efficient technology for analyzing odorous compounds, using various adsorbents and extracting solvents. The optimum adsorbent was XAD resins. Especially, SAD-2. XAD-7 and XAD-2010 were superior. but XAD-2 of these and the optimum extraction solvent is MTBE. Other extraction solvents' efficiency is in order of MTBE〉Dichloromethane〉n-Hexane〉Diethylether. The optimum NaCl dosage for increasing efficiency is 5g in liquid-liquid extraction method. The shaking time(0-24hr) has no concern with adsorption efficiency. The optimum adsorbent is SAD-2 resin and extraction solvent is MTBE. Dosing NaCl. adsorption efficiency is increased in liquid-liquid extraction method, but NaCl has no effect on liquid-solid extraction method. In this experimental results. this method will apply to not only Geosmin but other well-known odorous compounds (2-MIB, 1BMP. IPMP, TCA) and algae toxins (Mycrocystin. Anatoxin etc.).

석회석을 이용한 폐광산폐수처리

심은기,김은호,박인수,황영기 경남대학교 신소재연구소 1999 論文集 Vol.11 No.-

석회석을 이용하여 혐기성 처리를 위한 폐광산폐수의 화학적 전처리 특성을 평가하였다. 반응시간에 따른 석회석에 의한 처리시 반응종료 2시간에 pH 3.67이였고 SO42-4.7%, Fe 26%, Al 22%, Pb 18% 및 Mn 8%의 제거효율로서 소석회에 비하여 낮은 처리특성을 보여주고 있다. 석회석의 반응속도는 온도가 증가함에 따라 감소하는 경향을 보였다. 혐기성 석회석 충진층의 경우에 실험기간내 pH는 평균 4.51이였고 SO42-4.5%, Fe 15.3%, Al 20.1%, Pb 23.7% 및 Mn 5.87%의 평균 제거효율을 나타내었다. 혐기성 석회석 충진층은 pH 상승효과가 그다지 없었으며 SO42- 및 중금속제거율 또한 아주 낮아 석회석만으로 폐광산폐수의 처리에는 한계점이 있는 것으로 평가되었다. 그러나, 폐광산폐수를 SRB에 의한 처리시의 전처리 공정으로 활용한다면 강산성인 폐광산폐수를 초기에 pH를 상승시켜 처리계에 높은 알칼리도를 제공함으로서 시스템의 안정화에 기여할 수 있을 것으로 여겨진다. This research was carried out to investigate chemical pretreatment using limestone in treat in g abandoned mine drainage with anaerobic treatment. If treating limestone with abandoned mine drainage. after 2day. pH was increased to 3.67, and So₄^{2-}, Fe. Al. Pb and Mn were removed 4.7%. 26%. 22%. 18% and 8%. respectively. It could be showed that limestone did slowly react with temperature increasing. If treating anaerobic limestone packing column with abandoned mine drainage. for experimental period. average pH was 4.51, and average So₄^{2-}, Fe. Al, Pb and Mn were removed 4.5%. 15.3%. 20.1%. 23.7% and 5.87%. respectively. So, it would not be suitable for abandoned mine drainage. But if utilizing limestone as pre- treatment process for treating abandoned mine drainage with SRB, becaus it did initally neutralize abandoned mine drainage, it could forward to stabilize system.

오존을 이용한 조류제거에 관한 연구

김은호,심은기,박인수,황영기 경남대학교 신소재연구소 1999 論文集 Vol.11 No.-

본 연구에서는 이취를 유발하는 조류가 정수공정에 유입되었을 경우, 대표적인 고도종수처리 공정으로 알려진 오존처리공정에서 오존접촉시 pH, 총유기탄소(TOC), 자외부 발현성물질(UV-254) 및 NH4+-N 등의 변화특성을 조사하였다. pH가 초기에 7.4에서 오존접촉 10분까지는 급격한 pH 저하현상을 보인 후에 pH는 서서히 감소하는 현상을 보이며 오존접촉 100분에는 2.1까지 저하되었다. TOC와 UV-254는 오존과의 접촉시간에 따라 지속적으로 감소하는 경향을 나타내었다. NH4+-N와 NO2--N의 농도에 비해 NO3--N은 비교할 수 없을 정도의 높은 농도 차이를 보이고 있다. 오존접촉시간이 길어질수록 전기전도도 값도 높아지는 것을 알 수 있었다. The objectives of this research were to estimate variation characteristics of pH. TOC. UV_254 and NH₄^{+}-N. etc. if odorous generated algaes flowed into water treatment plant and they contacted with ozone known as typical advanced treatment. Initial pH 7.4 did suddenly decline to early l0min. and then pH drop did almost occur. and pH decreased to 2.1 in ozone contact 100min.. TOC and UV-254 continued to decrease with passed time of ozone contact. NO₃^{-}-N cocentration was much higher than NH₄^{+}-N and NO₂^{-}-N for whole test period. Conductivity showed high as passed time of ozone contact.

우리나라 파프리카 수출단지 및 선과장의 병해충 조사

김기돈,이시원,강은하,신용길,전재용,허노열,이흥식 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2

The disease and insect were surveyed locally in greenhouse, fruit packing house and store house of 51 farms in 13 towns having purpose of paprika exportation. By analysis, various disease and insect were not only founded locally but more ones detected in farms having old facilities and no natural enemy. We found 15 pathogens such as Fusarium spp., Alternaria solani, Leveilluila taurica, PepMV (Pepino mosaic virus) and TMV (Tobacco mosaic virus) in greenhouse, Fusarium spp. in fruit packing house and Penicillium spp. in store house. We found 15 insects in greenhouse such as Bemisia tabaci, rialeurodes vaporariorum and Myzus persicae in greenhouse, Hylobitelus haroldi in fruit packing house. However, the problem quarantine disease and insect for importation and exportation were not detected in inspection time.

딸기 와인 개발을 위한 최적 발효 조건

김민기, 박미성, 엄은경, 조은혜, 이종수 배재대학교 자연과학연구소 2016 自然科學論文集 Vol.27 No.1

Optimal fermentation condition for development of new strawberry wine were investigated. Commercial yeast, Fermivin was good for brewing of strawberry wine and its optimal addition concentration was also 1%. Maximal ethanol content of strawberry wine was obtained when raw strawberry juice(24 brix) was fermented by 1% Fermivin at 25℃ for 7 days and its antioxidant activity was 78.5%. 새로운 딸기 와인을 개발하기 위한 최적 발효조건을 연구하였다. 시판효모인 페미빈이 딸기와인 제조에 제일 좋았고 1%가 최적 첨가 농도이었다. 딸기와인의 최대 에탄올 생성량은 생딸기주스 (24brix)를 1% 페미빈으로 25℃에서 7일 발효시켰을 때 얻어졌고 이 발효액의 항산화활성은 78.5% 이었다.

구강환경에 따른 Streptococcus salivarius 의 요소분해활성의 변화

목지은,박상진,최기운,최호영 대한치과보존학회 2000 Restorative Dentistry & Endodontics Vol.25 No.4

Urea in the oral cavity is hydrolyzed mainly by bacterial ureases to ammonia, which in turn, raises pH of the oral environment, maintaining oral pH homeostasis, thereby inhibiting dental caries. Streptococcus salivarius has been shown to be a major contribution to oral ureolysis. Synthesis of urease by S. salivarius appears to be constitutive, but can be greatly enhanced in the acidic environment. It has been presumed that ureolytic activity of S. salivarius strains isolated from caries-active site is greater than that of strains from caries-free site. However, no in vivo study has supported the presumption. The present study was performed to observe the ureolytic activity of S. salivarius strains isolated from different environments in the same individual, finding out whether the ureolytic activity is related to dental caries. For the purpose, S. salivarius strains were isolated from caries-active site (>C2), a caries-free site of the tooth, and the dorsum of the tongue of each of 50 patients having decayed teeth. The strains isolated from the patients who harbored S. salivarius in more than two sites were selected and then their ureolytic activities were measured. In order to examine clonal diversity of the strains , their ureC genes were amplified by polymerase chain reaction (PCR) and then restricted with EcoRV, and the protein profiles of the strains were compared by SDS-PAGE. The results were as follows : 1.Of 50 patients, 13 patients harbored S. salivarius in more than two sites; a total of 61 S. salivarius strain were isolated from the patients and selected for the study. 2.Of 17 isolates from the caries-active site of 9 patients harboring S. salivarius in more than two sites including carious lesion, 10 (58.8%) showed a high ureolytic activity (> 200㎛ol/min/mg). While, 19 out of 44 isolates (43.2% ) from the caries-free site of the teeth and the dorsum of the tongues of 13 patients were the strains with a high ureolytic activity. 3.Of 9 patients harboring S. salivarius in more than two sites including caries-active site, 6 patients were found to have the strains in the caries-active site showing a lower ureolytic activity than the strains in the other sites.

공황장애의 임상적 특성

고은정,최영희,박기환,이정흠 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.2

Objectives : To clarify clinical features of panic disorder and to compare the characteristics of cognitive patterns, avoidance behaviors, and coping strategies among patients who have panic disorder with or without agoraphobia. Methods : Family backgrounds, cognitive patterns in the first panic attack, coping strategies, and avoidance behaviors were compared among the three groups classified by panic patients with mild agoraphobia(mild PDA=66), severe agoraphobia(severe PDA=71), and without agoraphobia(PD=21) Results : 1) Severe PDA reported significantly high panic attack frequency and more dysfunctional level than PD. 2) Catastrophic thinking of 'dying' in PD was significantly frequent than the other groups. Other fears of 'going craze or loss of control' in severe PDA were the highest among the three groups, though it was not significant. Specific thinking process or interpretation in the first panic attack was not significantly different among the three groups. 3) As avoidance behaviors became worse, the frequency of avoidance behaviors and the patters of avoidance behaviors increased. Conclusions : The results suggested that as avoidance behaviors became worse, the frequency of panic, maladaptive functioning and the patterns of avoidance behaviors increase. This might be why we need to decrease avoidance behaviors through exposure as the treatment.

오존과 광촉매를 이용한 수중 Geosimn 제거

황영기,김은호 경남대학교 경남지역문제연구원 2003 경남지역연구 Vol.9 No.-

성인학습자의 박물관 성인교육 프로그램 참여 및 저해 요인

전기선,최은수 한국성인교육학회 2007 Andragogy Today : International Journal of Adult & Vol.10 No.2

이 연구는 성인학습자의 박물관 성인교육 프로그램 참여에 관한 인식을 고찰하는데 그 목적을 두었다. 연구문제는 성인학습자의 참여 동기, 참여결정에 미치는 요인, 장애 요인, 선호하는 성인교육 프로그램의 종류에 대하여 조사하였다. 연구방법으로서 설문조사를 실시하였다. 설문조사는 국립중앙박물관과 국립민속박물관 성인교육 프로그램 참여자 275명을 대상으로 이루어 졌다. 연구결과에 따르면 성인학습자의 박물관 성인교육 프로그램 참여 동기는 새로운 인식과 경험확대를 위함이고, 참여결정에 미치는 요인은 도전해볼 학습내용이 가장 중요하게 고려되었으며, 원거리 및 교통 불편이 가장 큰 장애요인으로 나타났으며, 연중강의를 통한 심층적 지식확대를 위한 프로그램 종류를 가장 선호하였다. 박물관은 성인학습자의 관심과 요구를 면밀히 파악하여 이들의 내적 참여 동기를 통한 자발적인 참여를 유도할 수 있는 다양한 교육 프로그램을 개발하여 제공할 필요가 있었다. This study is to examine the adult learner's attitude and non-participation toward continuing education programs run designed by museums. For this purpose, the subjects such as motive, decisive factors, barriers and program preference were focused and examined with the survey. Questionnaires in survey were answered by 275 people who have experience in the programs of the National Museum of Korean and the National Folk Museum of Korea. According to the results, the motive to participate in museum program was driven by the desire to expand their knowledge and experience. The subject of program that is inclined to be challenging for the participant was a main factor when it comes to choose a program. The most chosen obstacle was the commute difficulty. As for the program preference, the program run for year long was chosen the most. The results above tell us that museums should pay more attention to what the adult learner's want most. And, eventually, museums should provide a wide variety of the education programs drawing voluntary attention from the learners.

삼각법을 기반으로한 구조화된 격자 캘리브레이션

김은석,주기세,왕지남 한국경영과학회 2002 한국경영과학회 학술대회논문집 Vol.- No.1(1)

Many sensors such as a structured grid pattern generator, a laser, and CCD camera to information have been used, but most of algorithms for a calibration are inefficient memory and experiment data are required. In this paper, the calibration algorithm of a structured grid pattern based on triang is introduced to calculate 3D information in the real world. The beams generated from str pattern generator established horizontally with the CCD camera are projected on the calibr CCD camera observes the intersection plane of a light and an object plane. The 3D infor calculated using observed and calibration data. This proposed method in this paper has advantages such as a memory saving and an e experimental data since the 3D information are obtained simply triangulation method.